Abstract:
BACKGROUND:Bifidobacterium spp. are representative probiotics that play an important role in the health of their hosts. Among various Bifidobacterium spp., B. bifidum BGN4 exhibits relatively high cell adhesion to colonic cells and has been reported to have various in vivo and in vitro bio functionalities (e.g., anti-allergic effect, anti-cancer effect, and modulatory effects on immune cells). Interleukin-10 (IL-10) has emerged as a major suppressor of immune response in macrophages and other antigen presenting cells and plays an essential role in the regulation and resolution of inflammation. In this study, recombinant B. bifidum BGN4 [pBESIL10] was developed to deliver human IL-10 effectively to the intestines. RESULTS:The vector pBESIL10 was constructed by cloning the human IL-10 gene under a gap promoter and signal peptide from Bifidobacterium spp. into the E. coli-Bifidobacterium shuttle vector pBES2. The secreted human IL-10 from B. bifidum BGN4 [pBESIL10] was analyzed by sodium dodecyl sulphate-polyacrylamide gel electrophoresis (SDS-PAGE), Western Blotting, and enzyme-linked immunosorbent assay (ELISA). More than 1,473 ± 300 ng/mL (n = 4) of human IL-10 was obtained in the cell free culture supernatant of B. bifidum BGN4 [pBESIL10]. This productivity is significantly higher than other previously reported human IL-10 level from food grade bacteria. In vitro functional evaluation of the cell free culture supernatant of B. bifidum BGN4 [pBESIL10] revealed significantly inhibited interleukin-6 (IL-6) production in lipopolysaccharide (LPS)-induced Raw 264.7 cells (n = 6, p < 0.0001) and interleukin-8 (IL-8) production in LPS-induced HT-29 cells (n = 6, p < 0.01) or TNFα-induced HT-29 cells (n = 6, p < 0.001). CONCLUSION:B. bifidum BGN4 [pBESIL10] efficiently produces and secretes significant amounts of biologically active human IL-10. The human IL-10 production level in this study is the highest of all human IL-10 production reported to date. Further research should be pursued to evaluate B. bifidum BGN4 [pBESIL10] producing IL-10 as a treatment for various inflammation-related diseases, including inflammatory bowel disease, rheumatoid arthritis, allergic asthma, and cancer immunotherapy.
journal_name
Microb Cell Factjournal_title
Microbial cell factoriesauthors
Hong N,Ku S,Yuk K,Johnston TV,Ji GE,Park MSdoi
10.1186/s12934-020-01505-ysubject
Has Abstractpub_date
2021-01-19 00:00:00pages
16issue
1issn
1475-2859pii
10.1186/s12934-020-01505-yjournal_volume
20pub_type
杂志文章abstract:BACKGROUND:Anaerobic digesters become unstable when operated at a high organi c loading rate (OLR). Investigating the microbial community response to OLR disturbance is helpful for achieving efficient and stable process operation. However, previous studies have only focused on community succession during different proc...
journal_title:Microbial cell factories
pub_type: 杂志文章
doi:10.1186/s12934-016-0466-y
更新日期:2016-04-25 00:00:00
abstract:BACKGROUND:Aspergillus terreus is a natural producer of itaconic acid and is currently used to produce itaconic acid on an industrial scale. The metabolic process for itaconic acid biosynthesis is very similar to the production of citric acid in Aspergillus niger. However, a key enzyme in A. niger, cis-aconitate decarb...
journal_title:Microbial cell factories
pub_type: 杂志文章
doi:10.1186/1475-2859-13-11
更新日期:2014-01-17 00:00:00
abstract:BACKGROUND:The methylotrophic yeast Pichia pastoris is a common host for the production of recombinant proteins. However, hypermannosylation hinders the use of recombinant proteins from yeast in most biopharmaceutical applications. Glyco-engineered yeast strains produce more homogeneously glycosylated proteins, but can...
journal_title:Microbial cell factories
pub_type: 杂志文章
doi:10.1186/s12934-018-1032-6
更新日期:2018-11-24 00:00:00
abstract:BACKGROUND:The application of chemical dispersants as a response to marine oil spills is raising concerns related to their potential toxicity also towards microbes involved in oil biodegradation. Hence, oil spills occurring under marine environments necessitate the application of biodispersants that are highly active, ...
journal_title:Microbial cell factories
pub_type: 杂志文章
doi:10.1186/s12934-017-0797-3
更新日期:2017-11-02 00:00:00
abstract:BACKGROUND:The methylotrophic yeast Pichia pastoris is a well-studied host organism for recombinant protein production, which is usually regulated either by a constitutive promoter (e.g. promoter of glyceraldehyde-3-phosphate dehydrogenase; PGAP) or an inducible promoter (e.g. promoter of alcohol oxidase 1; PAOX1). Bot...
journal_title:Microbial cell factories
pub_type: 杂志文章
doi:10.1186/s12934-017-0768-8
更新日期:2017-09-13 00:00:00
abstract:BACKGROUND:The expression of heterologous proteins in Escherichia coli is strongly affected by codon bias. This phenomenon occurs when the codon usage of the mRNA coding for the foreign protein differs from that of the bacterium. The ribosome pauses upon encountering a rare codon and may detach from the mRNA, thereby t...
journal_title:Microbial cell factories
pub_type: 杂志文章
doi:10.1186/1475-2859-8-41
更新日期:2009-07-24 00:00:00
abstract:INTRODUCTION:Chemical industries are constantly in search of an expeditious and environmentally benign method for producing chiral synthons. Ketoreductases have been used as catalysts for enantioselective conversion of desired prochiral ketones to their corresponding alcohol. We chose reported promiscuous ketoreductase...
journal_title:Microbial cell factories
pub_type: 杂志文章
doi:10.1186/s12934-018-1036-2
更新日期:2018-12-03 00:00:00
abstract::Pseudomonas sp. JHN decolourized and biotransformed 4-chloro-2-nitrophenol (4C2NP) in the presence of additional carbon source. The effect of the various concentrations of the 4C2NP was studied on the decolourization of 4C2NP by Pseudomonas sp. JHN. It was observed that strain JHN decolourized and biotransformed 4C2NP...
journal_title:Microbial cell factories
pub_type: 杂志文章
doi:10.1186/s12934-014-0110-7
更新日期:2014-08-12 00:00:00
abstract::This article describes the use of biofilm reactors for the production of various chemicals by fermentation and wastewater treatment. Biofilm formation is a natural process where microbial cells attach to the support (adsorbent) or form flocs/aggregates (also called granules) without use of chemicals and form thick lay...
journal_title:Microbial cell factories
pub_type: 杂志文章
doi:10.1186/1475-2859-4-24
更新日期:2005-08-25 00:00:00
abstract:BACKGROUND:Probiotic bacteria are increasingly used as immunomodulatory agents. Yet detailed molecular knowledge on the immunomodulatory molecules of these bacteria is lagging behind. Lipoteichoic acid (LTA) is considered a major microbe-associated molecular pattern (MAMP) of Gram-positive bacteria. However, many detai...
journal_title:Microbial cell factories
pub_type: 杂志文章
doi:10.1186/1475-2859-11-161
更新日期:2012-12-15 00:00:00
abstract:BACKGROUND:In the biopharmaceutical industry, Escherichia coli (E. coli) strains are among the most frequently used bacterial hosts for producing recombinant proteins because they allow a simple process set-up and they are Food and Drug Administration (FDA)-approved for human applications. Widespread use of E. coli in ...
journal_title:Microbial cell factories
pub_type: 杂志文章
doi:10.1186/1475-2859-12-58
更新日期:2013-06-11 00:00:00
abstract:BACKGROUND:The industrially relevant filamentous fungus Aspergillus niger is widely used in industry for its secretion capabilities of enzymes and organic acids. Biotechnologically produced organic acids promise to be an attractive alternative for the chemical industry to replace petrochemicals. Itaconic acid (IA) has ...
journal_title:Microbial cell factories
pub_type: 杂志文章
doi:10.1186/s12934-016-0527-2
更新日期:2016-07-28 00:00:00
abstract:BACKGROUND:C4-dicarboxylic acids, including malic acid, fumaric acid and succinic acid, are valuable organic acids that can be produced and secreted by a number of microorganisms. Previous studies on organic acid production by Aspergillus carbonarius, which is capable of producing high amounts of citric acid from varie...
journal_title:Microbial cell factories
pub_type: 杂志文章
doi:10.1186/s12934-017-0660-6
更新日期:2017-03-14 00:00:00
abstract:BACKGROUND:Raspberry ketone is the primary aroma compound found in raspberries and naturally derived raspberry ketone is a valuable flavoring agent. The economic incentives for the production of raspberry ketone, combined with the very poor yields from plant tissue, therefore make this compound an excellent target for ...
journal_title:Microbial cell factories
pub_type: 杂志文章
doi:10.1186/s12934-016-0446-2
更新日期:2016-03-04 00:00:00
abstract:BACKGROUND:Purine nucleotides exhibit various functions in cellular metabolism. Besides serving as building blocks for nucleic acid synthesis, they participate in signaling pathways and energy metabolism. Further, IMP and GMP represent industrially relevant biotechnological products used as flavor enhancing additives i...
journal_title:Microbial cell factories
pub_type: 杂志文章
doi:10.1186/1475-2859-11-138
更新日期:2012-10-24 00:00:00
abstract:BACKGROUND:It is quite important to understand how the central metabolism is regulated under nitrogen (N)- limitation as well as carbon (C)- limitation. In particular, the effect of C/N ratio on the metabolism is of practical interest for the heterologous protein production, PHB production, etc. Although the carbon and...
journal_title:Microbial cell factories
pub_type: 杂志文章
doi:10.1186/1475-2859-9-8
更新日期:2010-01-27 00:00:00
abstract:BACKGROUND:The filamentous fungus T. reesei effectively degrades cellulose and is known to produce various cellulolytic enzymes such as beta-glucosidase, endoglucanase, and cellobiohydrolase. The expression levels of each cellulase are controlled simultaneously, and their ratios and synergetic effects are important for...
journal_title:Microbial cell factories
pub_type: 杂志文章
doi:10.1186/1475-2859-9-32
更新日期:2010-05-14 00:00:00
abstract:BACKGROUND:Animal venoms are complex molecular cocktails containing a wide range of biologically active disulphide-reticulated peptides that target, with high selectivity and efficacy, a variety of membrane receptors. Disulphide-reticulated peptides have evolved to display improved specificity, low immunogenicity and t...
journal_title:Microbial cell factories
pub_type: 杂志文章
doi:10.1186/s12934-016-0617-1
更新日期:2017-01-17 00:00:00
abstract:BACKGROUND:Oxidized glutathione (GSSG) is the preferred form for industrial mass production of glutathione due to its high stability compared with reduced glutathione (GSH). In our previous study, over-expression of the mitochondrial thiol oxidase ERV1 gene was the most effective for high GSSG production in Saccharomyc...
journal_title:Microbial cell factories
pub_type: 杂志文章
doi:10.1186/s12934-017-0658-0
更新日期:2017-03-15 00:00:00
abstract:BACKGROUND:Methane-utilizing bacteria (methanotrophs) are capable of growth on methane and are attractive systems for bio-catalysis. However, the application of natural methanotrophic strains to large-scale production of value-added chemicals/biofuels requires a number of physiological and genetic alterations. An accur...
journal_title:Microbial cell factories
pub_type: 杂志文章
doi:10.1186/s12934-015-0377-3
更新日期:2015-11-25 00:00:00
abstract:BACKGROUND:We evaluated the functional capacity of plantaricin-producing Lactobacillus plantarum SF9C and S-layer-carrying Lactobacillus brevis SF9B to withstand gastrointestinal transit and to compete among the gut microbiota in vivo. Considering the probiotic potential of Lb. brevis SF9B, this study aims to investiga...
journal_title:Microbial cell factories
pub_type: 杂志文章
doi:10.1186/s12934-020-01365-6
更新日期:2020-05-19 00:00:00
abstract:BACKGROUND:The budding yeast Pichia pastoris is widely used for protein production. To determine the best suitable strategy for strain improvement, especially for high secretion, quantitative data of intracellular fluxes of recombinant protein are very important. Especially the balance between intracellular protein for...
journal_title:Microbial cell factories
pub_type: 杂志文章
doi:10.1186/1475-2859-10-47
更新日期:2011-06-26 00:00:00
abstract:BACKGROUND:Heterologous expression of biosynthetic gene clusters of natural microbial products has become an essential strategy for titer improvement and pathway engineering of various potentially-valuable natural products. A Streptomyces artificial chromosomal conjugation vector, pSBAC, was previously successfully app...
journal_title:Microbial cell factories
pub_type: 杂志文章
doi:10.1186/s12934-017-0708-7
更新日期:2017-05-31 00:00:00
abstract:BACKGROUND:Bacillus subtilis has been widely used as a host for heterologous protein expression in food industry. B. subtilis ATCC6051 is an alternative expression host for the production of industrial enzymes, and exhibits favorable growth properties compared to B. subtilis 168. Extracellular expression of pullulanase...
journal_title:Microbial cell factories
pub_type: 杂志文章
doi:10.1186/s12934-018-1011-y
更新日期:2018-10-22 00:00:00
abstract:BACKGROUND:Variations in codon usage between species are one of the major causes affecting recombinant protein expression levels, with a significant impact on the economy of industrial enzyme production processes. The use of codon-optimized genes may overcome this problem. However, designing a gene for optimal expressi...
journal_title:Microbial cell factories
pub_type: 杂志文章
doi:10.1186/1475-2859-10-15
更新日期:2011-03-03 00:00:00
abstract:BACKGROUND:The sesquiterpenoid abscisic acid (ABA) is mostly known for regulating developmental processes and abiotic stress responses in higher plants. Recent studies show that ABA also exhibits a variety of pharmacological activities. Affordable and sustainable production will be required to utilize the compound in a...
journal_title:Microbial cell factories
pub_type: 杂志文章
doi:10.1186/s12934-019-1257-z
更新日期:2019-11-25 00:00:00
abstract::Phytases are widely utilized in feed industry to increase the utilization of phosphorus, minerals, and amino acids for improvement of animal and human nutrition. At present, all known β-propeller phytases (BPP) have been generated from bacteria, particularly Bacillus. In this work we report for the first time a new fu...
journal_title:Microbial cell factories
pub_type: 杂志文章
doi:10.1186/s12934-020-01346-9
更新日期:2020-04-06 00:00:00
abstract:BACKGROUND:Genetic factors and a dysregulated immune response towards commensal bacteria contribute to the pathogenesis of Inflammatory Bowel Disease (IBD). Animal models demonstrated that the normal intestinal flora is crucial for the development of intestinal inflammation. However, due to the complexity of the intest...
journal_title:Microbial cell factories
pub_type: 杂志文章
doi:10.1186/1475-2859-6-18
更新日期:2007-06-06 00:00:00
abstract:BACKGROUND:In past years research has focused on the development of alternative Gram positive bacterial expression systems to produce industrially relevant proteins. Brevibacillus choshinensis is an easy to handle non-sporulating bacterium, lacking extracellular proteases, that has been already shown to provide a high ...
journal_title:Microbial cell factories
pub_type: 杂志文章
doi:10.1186/1475-2859-12-12
更新日期:2013-02-01 00:00:00
abstract:BACKGROUND:Corynebacterium glutamicum is a well-known producer of various L-amino acids in industry. During the fermenting process, C. glutamicum unavoidably encounters oxidative stress due to a specific reactive oxygen species (ROS) produced by consistent adverse conditions. To combat the ROS, C. glutamicum has develo...
journal_title:Microbial cell factories
pub_type: 杂志文章
doi:10.1186/s12934-018-1048-y
更新日期:2018-12-26 00:00:00