Abstract：
:This article describes the use of biofilm reactors for the production of various chemicals by fermentation and wastewater treatment. Biofilm formation is a natural process where microbial cells attach to the support (adsorbent) or form flocs/aggregates (also called granules) without use of chemicals and form thick layers of cells known as "biofilms." As a result of biofilm formation, cell densities in the reactor increase and cell concentrations as high as 74 gL(-1) can be achieved. The reactor configurations can be as simple as a batch reactor, continuous stirred tank reactor (CSTR), packed bed reactor (PBR), fluidized bed reactor (FBR), airlift reactor (ALR), upflow anaerobic sludge blanket (UASB) reactor, or any other suitable configuration. In UASB granular biofilm particles are used. This article demonstrates that reactor productivities in these reactors have been superior to any other reactor types. This article describes production of ethanol, butanol, lactic acid, acetic acid/vinegar, succinic acid, and fumaric acid in addition to wastewater treatment in the biofilm reactors. As the title suggests, biofilm reactors have high potential to be employed in biotechnology/bioconversion industry for viable economic reasons. In this article, various reactor types have been compared for the above bioconversion processes.
journal_name
Microb Cell Factjournal_title
Microbial cell factoriesauthors
Qureshi N,Annous BA,Ezeji TC,Karcher P,Maddox ISdoi
10.1186/1475-2859-4-24keywords:
subject
Has Abstractpub_date
2005-08-25 00:00:00pages
24issn
1475-2859pii
1475-2859-4-24journal_volume
4pub_type
杂志文章abstract：BACKGROUND:Recombinant antibodies can be produced in different formats and different expression systems. Single chain variable fragments (scFvs) represent an attractive alternative to full-length antibodies and they can be easily produced in bacteria or yeast. However, the scFvs exhibit monovalent antigen-binding prope...
journal_title：Microbial cell factories
pub_type： 杂志文章
doi：10.1186/1475-2859-10-109
更新日期：2011-12-15 00:00:00
abstract：BACKGROUND:Heterologous expression of biosynthetic gene clusters of natural microbial products has become an essential strategy for titer improvement and pathway engineering of various potentially-valuable natural products. A Streptomyces artificial chromosomal conjugation vector, pSBAC, was previously successfully app...
journal_title：Microbial cell factories
pub_type： 杂志文章
doi：10.1186/s12934-017-0708-7
更新日期：2017-05-31 00:00:00
abstract：:The biotechnology industry has become a key element in modern societies. Within this industry, the production of recombinant enzymes and biopharmaceutical proteins is of major importance. The global markets for such recombinant proteins are growing rapidly and, accordingly, there is a continuous need for new productio...
journal_title：Microbial cell factories
pub_type： 社论
doi：10.1186/1475-2859-12-3
更新日期：2013-01-14 00:00:00
abstract：BACKGROUND:Recent interest has been focused on the production of platform chemicals from renewable biomass due to increasing concerns on global warming and depletion of fossil fuel reserves. Microbial production of platform chemicals in biorefineries has been suggested to be a promising solution for these problems. Gam...
journal_title：Microbial cell factories
pub_type： 杂志文章
doi：10.1186/s12934-018-0977-9
更新日期：2018-08-21 00:00:00
abstract：BACKGROUND:The selection of bacterial recombinants that harbour a desired insert, has been a key factor in molecular cloning and a series of screening procedures need to be performed for selection of clones carrying the genes of interest. The conventional cloning techniques are reported to have problems such as screeni...
journal_title：Microbial cell factories
pub_type： 杂志文章
doi：10.1186/1475-2859-9-30
更新日期：2010-05-11 00:00:00
abstract：BACKGROUND:Cephalosporin C (CPC) produced by Acremonium chrysogenum is one of the most important drugs for treatment of bacterial infectious diseases. As the major stimulant, methionine is widely used in the industrial production of CPC. In this study, we found methionine stimulated CPC production through enhancing the...
journal_title：Microbial cell factories
pub_type： 杂志文章
doi：10.1186/s12934-018-0936-5
更新日期：2018-06-07 00:00:00
abstract：BACKGROUND:High-throughput methods are widely-used for strain screening effectively resulting in binary information regarding high or low productivity. Nevertheless achieving quantitative and scalable parameters for fast bioprocess development is much more challenging, especially for heterologous protein production. He...
journal_title：Microbial cell factories
pub_type： 杂志文章
doi：10.1186/1475-2859-11-144
更新日期：2012-10-31 00:00:00
abstract：BACKGROUND:A key advantage of recombinant antibody technology is the ability to optimize and tailor reagents. Single domain antibodies (sdAbs), the recombinantly produced variable domains derived from camelid and shark heavy chain antibodies, provide advantages of stability and solubility and can be further engineered ...
journal_title：Microbial cell factories
pub_type： 杂志文章
doi：10.1186/s12934-017-0837-z
更新日期：2017-12-12 00:00:00
abstract：BACKGROUND:Bioethanol is one of the most representative eco-friendly fuels developed to replace the non-renewable fossil fuels and is the most successful commercially available bio-conversion technology till date. With the availability of inexpensive carbon sources, such as cellulosic biomass, bioethanol production has...
journal_title：Microbial cell factories
pub_type： 杂志文章
doi：10.1186/s12934-019-1213-y
更新日期：2019-10-10 00:00:00
abstract：:Dunaliella salina is a unicellular green alga with a high α-linolenic acid (ALA) level, but a low eicosapentaenoic acid (EPA) level. In a previous analysis of the catalytic activity of delta 6 fatty acid desaturase (FADS6) from various species, FADS6 from Thalassiosira pseudonana (TpFADS6), a marine diatom, showed the...
journal_title：Microbial cell factories
pub_type： 杂志文章
doi：10.1186/s12934-018-0857-3
更新日期：2018-01-13 00:00:00
abstract：: Microbes utilize enzymes to perform a variety of functions. Enzymes are biocatalysts working as highly efficient machines at the molecular level. In the past, enzymes have been viewed as static entities and their function has been explained on the basis of direct structural interactions between the enzyme and the sub...
journal_title：Microbial cell factories
pub_type： 杂志文章
doi：10.1186/1475-2859-5-2
更新日期：2006-01-12 00:00:00
abstract：BACKGROUND:The unicellular cyanobacterium Synechocystis sp. PCC 6803 has been widely used as a photoautotrophic host for synthetic biology studies. However, as a green chassis to capture CO2 for biotechnological applications, the genetic toolbox for Synechocystis 6803 is still a limited factor. RESULTS:We systematical...
journal_title：Microbial cell factories
pub_type： 杂志文章
doi：10.1186/s12934-018-0897-8
更新日期：2018-03-26 00:00:00
abstract：BACKGROUND:Molecular mechanisms generating genetic variation provide the basis for evolution and long-term survival of a population in a changing environment. In stable, laboratory conditions, the variation-generating mechanisms are dispensable, as there is limited need for the cell to adapt to adverse conditions. In f...
journal_title：Microbial cell factories
pub_type： 杂志文章
doi：10.1186/1475-2859-11-11
更新日期：2012-01-20 00:00:00
abstract：:Controlling harmful algae blooms (HABs) using microbial algicides is cheap, efficient and environmental-friendly. However, obtaining high yield of algicidal microbes to meet the need of field test is still a big challenge since qualitative and quantitative analysis of algicidal compounds is difficult. In this study, w...
journal_title：Microbial cell factories
pub_type： 杂志文章
doi：10.1186/1475-2859-13-75
更新日期：2014-05-24 00:00:00
abstract：BACKGROUND:Nowadays, microbial infections have caused increasing economic losses in aquaculture industry and deteriorated worldwide environments. Many of these infections are caused by opportunistic pathogens through cell-density mediated quorum sensing (QS). The disruption of QS, known as quorum quenching (QQ), is an ...
journal_title：Microbial cell factories
pub_type： 杂志文章
doi：10.1186/s12934-020-01448-4
更新日期：2020-10-07 00:00:00
abstract：BACKGROUND:Production of heterologous proteins in the E. coli periplasm, or into the extracellular fluid has many advantages; therefore naturally occurring signal peptides are selected for proteins translocation. The aim of this study was the production in high yields of a recombinant pectin lyase that is efficiently s...
journal_title：Microbial cell factories
pub_type： 杂志文章
doi：10.1186/1475-2859-4-35
更新日期：2005-12-14 00:00:00
abstract：BACKGROUND:Streptomyces venezuelae ATCC 10712 produces antibiotics chloramphenicol (Cml) and jadomycin (Jad) in response to nutrient limitation and ethanol shock (ES), respectively. Biosynthesis of Cml and Jad was shown to be reciprocally regulated via the action of regulatory proteins JadR1 and JadR2 encoded by the ja...
journal_title：Microbial cell factories
pub_type： 杂志文章
doi：10.1186/s12934-016-0484-9
更新日期：2016-05-20 00:00:00
abstract：BACKGROUND:The deep-sea environment harbors a vast pool of novel enzymes. Owing to the limitations of cultivation, cultivation-independent has become an effective method for mining novel enzymes from the environment. Based on a deep-sea sediment metagenomics library, lipolytic-positive clones were obtained by activity-...
journal_title：Microbial cell factories
pub_type： 杂志文章
doi：10.1186/s12934-018-0864-4
更新日期：2018-01-30 00:00:00
abstract：BACKGROUND:In recent years, it has been gradually realized that bacterial inclusion bodies (IBs) could be biologically active. In particular, several proteins including green fluorescent protein, β-galactosidase, β-lactamase, alkaline phosphatase, D-amino acid oxidase, polyphosphate kinase 3, maltodextrin phosphorylase...
journal_title：Microbial cell factories
pub_type： 杂志文章
doi：10.1186/1475-2859-10-9
更新日期：2011-02-15 00:00:00
abstract：BACKGROUND:The industrially relevant filamentous fungus Aspergillus niger is widely used in industry for its secretion capabilities of enzymes and organic acids. Biotechnologically produced organic acids promise to be an attractive alternative for the chemical industry to replace petrochemicals. Itaconic acid (IA) has ...
journal_title：Microbial cell factories
pub_type： 杂志文章
doi：10.1186/s12934-016-0527-2
更新日期：2016-07-28 00:00:00
abstract：BACKGROUND:Colorectal cancer (CRC) is a major clinical challenge, and the gut microbiome plays important roles in the occurrence and metastasis of CRC. Lactobacillus and their metabolites are thought to be able to suppress the growth of CRC cells. However, the antimetastatic mechanism of Lactobacillus or their metaboli...
journal_title：Microbial cell factories
pub_type： 杂志文章
doi：10.1186/s12934-020-01466-2
更新日期：2020-11-23 00:00:00
abstract：BACKGROUND:Bacillus subtilis is an important industrial workhorse applied in the production of many different commercially relevant proteins, especially enzymes. Virtually all of these proteins are secreted via the general secretion (Sec) pathway. Studies from different laboratories have demonstrated essential or non-e...
journal_title：Microbial cell factories
pub_type： 杂志文章
doi：10.1186/s12934-020-01315-2
更新日期：2020-02-28 00:00:00
abstract：BACKGROUND:The sesquiterpenoid abscisic acid (ABA) is mostly known for regulating developmental processes and abiotic stress responses in higher plants. Recent studies show that ABA also exhibits a variety of pharmacological activities. Affordable and sustainable production will be required to utilize the compound in a...
journal_title：Microbial cell factories
pub_type： 杂志文章
doi：10.1186/s12934-019-1257-z
更新日期：2019-11-25 00:00:00
abstract：BACKGROUND:cis, cis-Muconic acid is an important chemical that can be biosynthesized from simple substrates in engineered microorganisms. Recently, it has been shown that engineering microbial cocultures is an emerging and promising approach for biochemical production. In this study, we aim to explore the potential of ...
journal_title：Microbial cell factories
pub_type： 杂志文章
doi：10.1186/s12934-015-0319-0
更新日期：2015-09-15 00:00:00
abstract：BACKGROUND:Recombinant protein production is universally employed as a solution to obtain the milligram to gram quantities of a given protein required for applications as diverse as structural genomics and biopharmaceutical manufacture. Yeast is a well-established recombinant host cell for these purposes. In this study...
journal_title：Microbial cell factories
pub_type： 杂志文章
doi：10.1186/1475-2859-9-47
更新日期：2010-06-17 00:00:00
abstract：BACKGROUND:Succinic acid is well established as bio-based platform chemical with production quantities expecting to increase exponentially within the next decade. Actinobacillus succinogenes is by far the most studied wild organism for producing succinic acid and is known for high yield and titre during production on v...
journal_title：Microbial cell factories
pub_type： 杂志文章
doi：10.1186/s12934-014-0111-6
更新日期：2014-08-19 00:00:00
abstract：BACKGROUND:The methylotrophic yeast Pichia pastoris is a well-studied host organism for recombinant protein production, which is usually regulated either by a constitutive promoter (e.g. promoter of glyceraldehyde-3-phosphate dehydrogenase; PGAP) or an inducible promoter (e.g. promoter of alcohol oxidase 1; PAOX1). Bot...
journal_title：Microbial cell factories
pub_type： 杂志文章
doi：10.1186/s12934-017-0768-8
更新日期：2017-09-13 00:00:00
abstract：BACKGROUND:The solubility of recombinant proteins expressed in bacteria is often disappointingly low. Several strategies have been developed to improve the yield and one of the most common strategies is the fusion of the target protein with a suitable partner. Despite several reports on the successful use of each of th...
journal_title：Microbial cell factories
pub_type： 杂志文章
doi：10.1186/1475-2859-4-34
更新日期：2005-12-13 00:00:00
abstract：:A growing number of insights on the biology of bacterial inclusion bodies (IBs) have revealed intriguing utilities of these protein particles. Since they combine mechanical stability and protein functionality, IBs have been already exploited in biocatalysis and explored for bottom-up topographical modification in tiss...
journal_title：Microbial cell factories
pub_type： 杂志文章
doi：10.1186/1475-2859-11-76
更新日期：2012-06-11 00:00:00
abstract：BACKGROUND:The expression of heterologous proteins in Escherichia coli is strongly affected by codon bias. This phenomenon occurs when the codon usage of the mRNA coding for the foreign protein differs from that of the bacterium. The ribosome pauses upon encountering a rare codon and may detach from the mRNA, thereby t...
journal_title：Microbial cell factories
pub_type： 杂志文章
doi：10.1186/1475-2859-8-41
更新日期：2009-07-24 00:00:00
