Abstract:
BACKGROUND:Mucosal delivery of therapeutic proteins using genetically modified strains of lactic acid bacteria (gmLAB) is being investigated as a new therapeutic strategy. METHODS:We developed a strain of gmLAB, Lactococcus lactis NZ9000 (NZ-HO), which secretes the anti-inflammatory molecule recombinant mouse heme oxygenase-1 (rmHO-1). The effects of short-term continuous oral dosing with NZ-HO were evaluated in mice with dextran sulfate sodium (DSS)-induced acute colitis as a model of inflammatory bowel diseases (IBD). RESULTS:We identified the secretion of rmHO-1 by NZ-HO. rmHO-1 was biologically active as determined with spectroscopy. Viable NZ-HO was directly delivered to the colon via oral administration, and rmHO-1 was secreted onto the colonic mucosa in mice. Acute colitis in mice was induced by free drinking of 3 % DSS in water and was accompanied by an increase in the disease activity index score and histopathological changes. Daily oral administration of NZ-HO significantly improved these colitis-associated symptoms. In addition, NZ-HO significantly increased production of the anti-inflammatory cytokine interleukin (IL)-10 and decreased the expression of pro-inflammatory cytokines such as IL-1α and IL-6 in the colon compared to a vector control strain. CONCLUSIONS:Oral administration of NZ-HO alleviates DSS-induced acute colitis in mice. Our results suggest that NZ-HO may be a useful mucosal therapeutic agent for treating IBD.
journal_name
Microb Cell Factjournal_title
Microbial cell factoriesauthors
Shigemori S,Watanabe T,Kudoh K,Ihara M,Nigar S,Yamamoto Y,Suda Y,Sato T,Kitazawa H,Shimosato Tdoi
10.1186/s12934-015-0378-2subject
Has Abstractpub_date
2015-11-25 00:00:00pages
189issn
1475-2859pii
10.1186/s12934-015-0378-2journal_volume
14pub_type
杂志文章abstract:BACKGROUND:Streptomycetes produce a plethora of natural products including antibiotics and anticancer drugs, as well as many industrial enzymes. Their mycelial life style is a major bottleneck for industrial exploitation and over decades strain improvement programs have selected production strains with better growth pr...
journal_title:Microbial cell factories
pub_type: 杂志文章
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更新日期:2015-04-01 00:00:00
abstract:BACKGROUND:High-throughput screening methods assume that the output measured is representative of changes in metabolic flux toward the desired product and is not affected by secondary phenotypes. However, metabolic engineering can result in unintended phenotypes that may go unnoticed in initial screening. The red pigme...
journal_title:Microbial cell factories
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abstract:BACKGROUND:Yeast viability and vitality are essential for different industrial processes where the yeast Saccharomyces cerevisiae is used as a biotechnological tool. Therefore, the decline of yeast biological functions during aging may compromise their successful biotechnological use. Life span is controlled by a varie...
journal_title:Microbial cell factories
pub_type: 杂志文章
doi:10.1186/1475-2859-12-1
更新日期:2013-01-02 00:00:00
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journal_title:Microbial cell factories
pub_type: 杂志文章
doi:10.1186/s12934-016-0466-y
更新日期:2016-04-25 00:00:00
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journal_title:Microbial cell factories
pub_type: 杂志文章
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journal_title:Microbial cell factories
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journal_title:Microbial cell factories
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journal_title:Microbial cell factories
pub_type: 杂志文章
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更新日期:2010-11-13 00:00:00
abstract:BACKGROUND:Fumaric acid is a commercially important component of foodstuffs, pharmaceuticals and industrial materials, yet the current methods of production are unsustainable and ecologically destructive. RESULTS:In this study, the fumarate biosynthetic pathway involving reductive reactions of the tricarboxylic acid c...
journal_title:Microbial cell factories
pub_type: 杂志文章
doi:10.1186/1475-2859-11-24
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journal_title:Microbial cell factories
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journal_title:Microbial cell factories
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更新日期:2019-03-28 00:00:00
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journal_title:Microbial cell factories
pub_type: 杂志文章
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journal_title:Microbial cell factories
pub_type: 社论
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更新日期:2010-07-05 00:00:00
abstract:BACKGROUND:Aspergillus niger is widely used for enzyme production and achievement of high enzyme production depends on the comprehensive understanding of cell's metabolic regulation mechanisms. RESULTS:In this paper, we investigate the metabolic differences and regulation mechanisms between a high glucoamylase-produci...
journal_title:Microbial cell factories
pub_type: 杂志文章
doi:10.1186/s12934-015-0329-y
更新日期:2015-09-17 00:00:00
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journal_title:Microbial cell factories
pub_type: 杂志文章
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更新日期:2016-08-11 00:00:00
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journal_title:Microbial cell factories
pub_type: 杂志文章
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更新日期:2019-09-21 00:00:00
abstract:BACKGROUND:Raspberry ketone is the primary aroma compound found in raspberries and naturally derived raspberry ketone is a valuable flavoring agent. The economic incentives for the production of raspberry ketone, combined with the very poor yields from plant tissue, therefore make this compound an excellent target for ...
journal_title:Microbial cell factories
pub_type: 杂志文章
doi:10.1186/s12934-016-0446-2
更新日期:2016-03-04 00:00:00
abstract:BACKGROUND:Microbial genome editing is a powerful tool to modify chromosome in way of deletion, insertion or replacement, which is one of the most important techniques in metabolic engineering research. The emergence of CRISPR/Cas9 technique inspires various genomic editing methods. RESULTS:In this research, the goal ...
journal_title:Microbial cell factories
pub_type: 杂志文章
doi:10.1186/s12934-016-0605-5
更新日期:2016-12-01 00:00:00
abstract:BACKGROUND:Catalase (EC 1.11.1.6) is one of the important industrial enzyme employed in diagnostic and analytical methods in the form of biomarkers and biosensors in addition to their enormous applications in textile, paper, food and pharmaceutical sectors. The present study demonstrates the utility of a newly isolated...
journal_title:Microbial cell factories
pub_type: 杂志文章
doi:10.1186/s12934-016-0410-1
更新日期:2016-01-14 00:00:00
abstract:BACKGROUND:Activation of silent biosynthetic gene clusters (BGCs) in marine-derived actinomycete strains is a feasible strategy to discover bioactive natural products. Actinoalloteichus sp. AHMU CJ021, isolated from the seashore, was shown to contain an intact but silent caerulomycin A (CRM A) BGC-cam in its genome. Th...
journal_title:Microbial cell factories
pub_type: 杂志文章
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更新日期:2020-08-06 00:00:00
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pub_type: 杂志文章
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abstract:BACKGROUND:Strains with increased alkali tolerance have a broad application in industrial, especially for bioremediation, biodegradation, biocontrol and production of bio-based chemicals. A novel synthetic chromosome recombination and modification by LoxP-mediated evolution (SCRaMbLE) system has been introduced in the ...
journal_title:Microbial cell factories
pub_type: 杂志文章
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journal_title:Microbial cell factories
pub_type: 杂志文章
doi:10.1186/s12934-017-0788-4
更新日期:2017-10-10 00:00:00
abstract:BACKGROUND:Bifidobacterium spp. are representative probiotics that play an important role in the health of their hosts. Among various Bifidobacterium spp., B. bifidum BGN4 exhibits relatively high cell adhesion to colonic cells and has been reported to have various in vivo and in vitro bio functionalities (e.g., anti-a...
journal_title:Microbial cell factories
pub_type: 杂志文章
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更新日期:2021-01-19 00:00:00
abstract:BACKGROUND:Fatty aldehydes are industrially relevant compounds, which also represent a common metabolic intermediate in the microbial synthesis of various oleochemicals, including alkanes, fatty alcohols and wax esters. The key enzymes in biological fatty aldehyde production are the fatty acyl-CoA/ACP reductases (FARs)...
journal_title:Microbial cell factories
pub_type: 杂志文章
doi:10.1186/s12934-018-0869-z
更新日期:2018-02-08 00:00:00
abstract:BACKGROUND:Phasin (PhaP), a kind of polyhydroxyalkanoate (PHA) granule-associated proteins, has a role in controlling the properties of PHA granules surface, and is thought to have influence on PHA biosynthesis in PHA-producing bacteria. This study focused on the phaP1(Re) locus in Ralstonia eutropha as a site of chrom...
journal_title:Microbial cell factories
pub_type: 杂志文章
doi:10.1186/s12934-015-0380-8
更新日期:2015-11-23 00:00:00
abstract:BACKGROUND:The oleaginous yeast Yarrowia lipolytica is an organism of choice for the tailored production of various compounds such as biofuels or biopolymers. When properly engineered, it is capable of producing medium-chain-length polyhydroxyalkanoate (mcl-PHA), a biobased and biodegradable polymer that can be used as...
journal_title:Microbial cell factories
pub_type: 杂志文章
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更新日期:2019-05-31 00:00:00
abstract:BACKGROUND:Bacillus subtilis is widely used for the industrial production of recombinant proteins, mainly due to its high secretion capacity, but higher production yields can be achieved only if bottlenecks are removed. To this end, a crucial process is translation initiation which takes place at the ribosome binding s...
journal_title:Microbial cell factories
pub_type: 杂志文章
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更新日期:2020-07-29 00:00:00
abstract:BACKGROUND:Thymosin α1 (Tα1), a 28-amino acid Nα-acetylated peptide, has a powerful general immunostimulating activity. Although biosynthesis is an attractive means of large-scale manufacture, to date, Tα1 can only be chemosynthesized because of two obstacles to its biosynthesis: the difficulties in expressing small pe...
journal_title:Microbial cell factories
pub_type: 杂志文章
doi:10.1186/1475-2859-10-26
更新日期:2011-04-22 00:00:00