Abstract：
:We developed a new technique to observe macroautophagy in the brain in vivo, and examined whether fasting induced macroautophagy in neurons and how the induction was different between Alzheimer's disease (AD) model and control mice. Lentivirus for EGFP-LC3 injected into the brain successfully visualized autophagosome in living neurons by two-photon microscopy. The time-lapse imaging revealed that fasting increased the number, size and signal intensity of autophagosome in neurons. In AD model mice, these parameters of autophagosome were higher at the basal levels before starvation, and increased more rapidly by fasting than in control mice. However, metabolism of exogenous labeled Aβ evaluated by the new technique suggested that the activated macroautophagy was insufficient to degrade the intracellular Aβ increased by enhanced uptake from extracellular space after fasting. Ordinary immunohistochemistry also revealed that fasting increased intracellular accumulation of endogenous Aβ, triggered cell dysfunction but did not mostly decrease extracellular Aβ accumulation. Moreover, we unexpectedly discovered a circadian rhythm of basal level of macroautophagy. These results revealed new aspects of neuronal autophagy in normal/AD states and indicated usefulness of our method for evaluating autophagy functions in vivo.
journal_name
Sci Repjournal_title
Scientific reportsauthors
Chen X,Kondo K,Motoki K,Homma H,Okazawa Hdoi
10.1038/srep12115subject
Has Abstractpub_date
2015-07-14 00:00:00pages
12115issn
2045-2322pii
srep12115journal_volume
5pub_type
杂志文章abstract：:Novel activated boron nitride (BN) as an effective adsorbent for pollutants in water and air has been reported in the present work. The activated BN was synthesized by a simple structure-directed method that enabled us to control the surface area, pore volume, crystal defects and surface groups. The obtained BN exhibi...
journal_title：Scientific reports
pub_type： 杂志文章
doi：10.1038/srep03208
更新日期：2013-11-13 00:00:00
abstract：:Caspase-1, also known as interleukin-1β (IL-1β)-converting enzyme (ICE), regulates antimicrobial host defense, tissue repair, tumorigenesis, metabolism and membrane biogenesis. On activation within an inflammasome complex, caspase-1 induces pyroptosis and converts pro-IL-1β and pro-IL-18 into their biologically active...
journal_title：Scientific reports
pub_type： 杂志文章
doi：10.1038/srep45126
更新日期：2017-03-27 00:00:00
abstract：:Surface plasmon resonators can drastically redistribute incident light over different output wave vectors and polarizations. This can lead for instance to sub-diffraction sized nanoapertures in metal films that beam and to nanoparticle antennas that enable efficient conversion of photons between spatial modes, or heli...
journal_title：Scientific reports
pub_type： 杂志文章
doi：10.1038/srep09966
更新日期：2015-04-30 00:00:00
abstract：:Smad1, Smad5 and Smad9 (also known as Smad8) are activated by phosphorylation by bone morphogenetic protein (BMP)-bound type I receptor kinases. We examined the role of Smad1, Smad5, and Smad9 by creating constitutively active forms (Smad(DVD)). Transcriptional activity of Smad9(DVD) was lower than that of Smad1(DVD) ...
journal_title：Scientific reports
pub_type： 杂志文章
doi：10.1038/srep07596
更新日期：2014-12-23 00:00:00
abstract：:Incomplete or partial observations of network structures pose a serious challenge to theoretical and engineering studies of real networks. To remedy the missing links in real datasets, topology-based link prediction is introduced into the studies of various networks. Due to the complexity of network structures, the ac...
journal_title：Scientific reports
pub_type： 杂志文章
doi：10.1038/s41598-018-31254-3
更新日期：2018-09-06 00:00:00
abstract：:The cholinergic system plays a major anti-inflammatory role in many diseases through acetylcholine (Ach) release after vagus nerve stimulation. Osteoarthritis (OA) is associated with local low-grade inflammation, but the regulatory mechanisms are unclear. Local Ach release could have anti-inflammatory activity since a...
journal_title：Scientific reports
pub_type： 杂志文章
doi：10.1038/s41598-020-65873-6
更新日期：2020-06-01 00:00:00
abstract：:We present a proposal to realize the quantum Zeno effect (QZE) and quantum Zeno-like effect (QZLE) in a proximal (13)C nuclear spin by controlling a proximal electron spin of a nitrogen vacancy (NV) center. The measurement is performed by applying a microwave pulse to induce the transition between different electronic...
journal_title：Scientific reports
pub_type： 杂志文章
doi：10.1038/srep17615
更新日期：2015-12-01 00:00:00
abstract：:The present study evaluates the potential of Sar gassum muticum (Sar) and Jan ia rubens (Jan) seaweeds for enhancing growth and mitigating soil-salinity in chickpea (Cicer arietinum L.). Under control conditions, Sar and Jan extracts improved chickpea growth which was attributed to their potential for increasing photo...
journal_title：Scientific reports
pub_type： 杂志文章
doi：10.1038/s41598-017-07692-w
更新日期：2017-09-05 00:00:00
abstract：:A forest of the black coral Antipathella subpinnata was found from 52 to 80 m depth in three different sites at Tremiti Islands Marine Protected Area (MPA; Mediterranean Sea), with two of them hosting a monospecific forest on horizontal and vertical substrates. Colonies of A. subpinnata showed a mean density between 0...
journal_title：Scientific reports
pub_type： 杂志文章
doi：10.1038/s41598-020-65266-9
更新日期：2020-05-22 00:00:00
abstract：:Diagnostic test accuracy of the loop-mediated isothermal amplification (LAMP) assay for culture proven tuberculosis is unclear. We searched electronic databases for both cohort and case-control studies that provided data to calculate sensitivity and specificity. The index test was any LAMP assay including both commerc...
journal_title：Scientific reports
pub_type： 杂志文章,meta分析,评审
doi：10.1038/srep39090
更新日期：2016-12-13 00:00:00
abstract：:Fungal endophytes isolated from mountain-cultivated ginseng (MCG, Panax ginseng Meyer) were explored for their diversity and biocontrol activity against ginseng pathogens (Alternaria panax, Botrytis cinerea, Cylindrocarpon destructans, Pythium sp. and Rhizoctonia solani). A total of 1,300 isolates were isolated from t...
journal_title：Scientific reports
pub_type： 杂志文章
doi：10.1038/s41598-017-16181-z
更新日期：2017-11-24 00:00:00
abstract：:Climate warming is likely to interact with other stressors to challenge the physiological capacities and survival of phenotypes within populations. This may be especially true for the billions of fishes per year that undergo vigorous exercise prior to escaping or being intentionally released from fishing gear. Using a...
journal_title：Scientific reports
pub_type： 杂志文章
doi：10.1038/srep40571
更新日期：2017-01-17 00:00:00
abstract：:We investigate the impact of borders on the topology of spatially embedded networks. Indeed territorial subdivisions and geographical borders significantly hamper the geographical span of networks thus playing a key role in the formation of network communities. This is especially important in scientific and technologi...
journal_title：Scientific reports
pub_type： 杂志文章
doi：10.1038/srep04546
更新日期：2014-04-01 00:00:00
abstract：:To gain the fundamental understanding of deformation mechanisms in an aluminum-containing austenitic high-Mn steel (Fe-32Mn-8.9Al-0.78 C (wt.%)), in-situ straining transmission electron microscopy (TEM) analysis is conducted. The in-situ observation during the deformation demonstrates that the plastic deformation is a...
journal_title：Scientific reports
pub_type： 杂志文章
doi：10.1038/s41598-019-51586-y
更新日期：2019-10-23 00:00:00
abstract：:Ionotropic activation of NMDA receptors (NMDARs) requires agonist glutamate and co-agonist glycine. Here we show that glycine enhances the activation of cell survival-promoting kinase Akt in cultured cortical neurons in which both the channel activity of NMDARs and the glycine receptors are pre-inhibited. The effect o...
journal_title：Scientific reports
pub_type： 杂志文章
doi：10.1038/srep34459
更新日期：2016-10-03 00:00:00
abstract：:Dirt and microorganisms are the major problems in textiles which can generate unpleasant odor during their growth. Here, zinc oxide (ZnO) nanoparticles prepared by sol-gel method were loaded on the cotton fabrics using spin coating technique to enhance their antimicrobial properties and water repellency. The effects o...
journal_title：Scientific reports
pub_type： 杂志文章
doi：10.1038/s41598-018-22324-7
更新日期：2018-03-02 00:00:00
abstract：:Propagation of coherent light through a disordered network is accompanied by randomization and possible conversion into thermal light. Here, we show that network topology plays a decisive role in determining the statistics of the emerging field if the underlying lattice is endowed with chiral symmetry. In such lattice...
journal_title：Scientific reports
pub_type： 杂志文章
doi：10.1038/s41598-017-09236-8
更新日期：2017-08-21 00:00:00
abstract：:Type 2 Diabetes (T2D) is increasing but the effectiveness of large-scale diabetes screening programmes is debated. We assessed associations between coverage of a national cardiovascular and diabetes risk assessment programme in England (NHS Health Check) and detection and management of incident cases of non-diabetic h...
journal_title：Scientific reports
pub_type： 杂志文章
doi：10.1038/s41598-020-58033-3
更新日期：2020-01-27 00:00:00
abstract：:L-Ascorbic acid (vitamin C, AA) exhibits anti-cancer effects with high-dose treatment through the generation of reactive oxygen species (ROS) and selective damage to cancer cells. The anti-cancer effects of L-ascorbic acid are determined by sodium-dependent vitamin C transporter 2 (SVCT-2), a transporter of L-ascorbic...
journal_title：Scientific reports
pub_type： 杂志文章
doi：10.1038/s41598-018-29386-7
更新日期：2018-07-27 00:00:00
abstract：:cAMP responsive element binding protein 3-like 3 (CREB3L3), a transcription factor expressed in the liver and small intestine, governs fasting-response energy homeostasis. Tissue-specific CREB3L3 knockout mice have not been generated till date. To our knowledge, this is the first study using the one-step CRISPR/Cas9 s...
journal_title：Scientific reports
pub_type： 杂志文章
doi：10.1038/srep27857
更新日期：2016-06-13 00:00:00
abstract：:Cell motility requires the precise coordination of cell polarization, lamellipodia formation, adhesion, and force generation. LKB1 is a multi-functional serine/threonine kinase that associates with actin at the cellular leading edge of motile cells and suppresses FAK. We sought to understand how LKB1 coordinates these...
journal_title：Scientific reports
pub_type： 杂志文章
doi：10.1038/srep40929
更新日期：2017-01-19 00:00:00
abstract：:Work stress is a risk factor for cardio-metabolic diseases, but few large-scale studies have examined the clinical profile of individuals with work stress. To address this limitation, we conducted a cross-sectional study including 43,593 working adults from a French population-based sample aged 18-72 years (the CONSTA...
journal_title：Scientific reports
pub_type： 杂志文章
doi：10.1038/s41598-017-07508-x
更新日期：2017-08-24 00:00:00
abstract：:Lytic bacteriophages are valuable therapeutic agents against bacterial infections. There is continual effort to obtain new phages to increase the effectivity of phage preparations against emerging phage-resistant strains. Here we described the genomic diversity of spontaneous host-range mutants of kayvirus 812. Five m...
journal_title：Scientific reports
pub_type： 杂志文章
doi：10.1038/s41598-019-41868-w
更新日期：2019-04-02 00:00:00
abstract：:We report fabrication of large area Ag nanowire (NW) film coated using a continuous roll-to-roll (RTR) slot die coater as a viable alternative to conventional ITO electrodes for cost-effective and large-area flexible touch screen panels (TSPs). By controlling the flow rate of shear-thinning Ag NW ink in the slot die, ...
journal_title：Scientific reports
pub_type： 杂志文章
doi：10.1038/srep34322
更新日期：2016-09-28 00:00:00
abstract：:We report on a combined photoluminescence imaging and atomic force microscopy study of single, isolated self-assembled InAs quantum dots. The motivation of this work is to determine an approach that allows to assess single quantum dots as candidates for quantum nanophotonic devices. By combining optical and scanning p...
journal_title：Scientific reports
pub_type： 杂志文章
doi：10.1038/s41598-017-06566-5
更新日期：2017-07-24 00:00:00
abstract：:We investigated whether maternal metabolic environment affects mesenchymal stromal/stem cells (MSCs) from umbilical cord's Wharton's Jelly (WJ) on a molecular level, and potentially render them unsuitable for clinical use in multiple recipients. In this pilot study on umbilical cords post partum from healthy non-obese...
journal_title：Scientific reports
pub_type： 杂志文章
doi：10.1038/s41598-017-18034-1
更新日期：2017-12-14 00:00:00
abstract：:Phototherapy was introduced in the early 1950's, and is the primary treatment of severe neonatal jaundice or Crigler-Najjar syndrome. Nevertheless, the potential biological effects of the products generated from the photodegradation of bilirubin during phototherapy remain unknown. This is very relevant in light of rec...
journal_title：Scientific reports
pub_type： 杂志文章
doi：10.1038/s41598-018-25684-2
更新日期：2018-05-10 00:00:00
abstract：:Water-saving and drought-resistantce rice (WDR) breeding practices have greatly increased grain yield and drought resistance. To study the genetic basis of adaptation to drought, transcriptome sequences from the WDR maintainer line HuHan2B and the recurrent parent HanFengB were analyzed for alternative splicing (AS) c...
journal_title：Scientific reports
pub_type： 杂志文章
doi：10.1038/s41598-017-12020-3
更新日期：2017-09-15 00:00:00
abstract：:The honeybee's drinking process has generally been simplified because of its high speed and small scale. In this study, we clearly observed the drinking cycle of the Italian honeybee using a specially designed high-speed camera system. We analysed the pattern of glossal hair erection and the movement kinematics of the...
journal_title：Scientific reports
pub_type： 杂志文章
doi：10.1038/srep15002
更新日期：2015-10-08 00:00:00
abstract：:Layered Sn-based chalcogenides and heterostructures are widely used in batteries and photocatalysis, but its utilizations in a supercapacitor is limited by its structural instability and low conductivity. Here, SnSx thin films are directly and conformally deposited on a three-dimensional (3D) Ni-foam (NF) substrate by...
journal_title：Scientific reports
pub_type： 杂志文章
doi：10.1038/s41598-019-46679-7
更新日期：2019-07-15 00:00:00
