Abstract:
:The cholinergic system plays a major anti-inflammatory role in many diseases through acetylcholine (Ach) release after vagus nerve stimulation. Osteoarthritis (OA) is associated with local low-grade inflammation, but the regulatory mechanisms are unclear. Local Ach release could have anti-inflammatory activity since articular cells express Ach receptors involved in inflammatory responses. Using the 3DISCO clearing protocol that allows whole-sample 3-dimensional (3D) analysis, we cleared human OA cartilage-subchondral bone samples to search for cholinergic nerve fibres able to produce Ach locally. We analysed 3 plugs of knee cartilage and subchondral bone from 3 OA patients undergoing arthroplasty. We found no nerves in the superficial and intermediate articular cartilage layers, as evidenced by the lack of Peripherin staining (a peripheral nerves marker). Conversely, peripheral nerves were found in the deepest layer of cartilage and in subchondral bone. Some nerves in the subchondral bone samples were cholinergic because they coexpressed peripherin and choline acetyltransferase (ChAT), a specific marker of cholinergic nerves. However, no cholinergic nerves were found in the cartilage layers. It is therefore feasible to clear human bone to perform 3D immunofluorescence. Human OA subchondral bone is innervated by cholinergic fibres, which may regulate local inflammation through local Ach release.
journal_name
Sci Repjournal_title
Scientific reportsauthors
Courties A,Belle M,Senay S,Cambon-Binder A,Sautet A,Chédotal A,Berenbaum F,Sellam Jdoi
10.1038/s41598-020-65873-6subject
Has Abstractpub_date
2020-06-01 00:00:00pages
8852issue
1issn
2045-2322pii
10.1038/s41598-020-65873-6journal_volume
10pub_type
杂志文章abstract::Effective promotion of air filtration applications proposed for fibers requires their real nanoscale diameter, optimized pore structure, and high service strength; however, creating such filter medium has proved to be a tremendous challenge. This study first establishes a strategy to design and fabricate novel poly(m-...
journal_title:Scientific reports
pub_type: 杂志文章
doi:10.1038/srep40550
更新日期:2017-01-11 00:00:00
abstract::Mammals typically keep their body temperature (Tb) within a narrow limit with changing environmental conditions. There are indications that some wild ungulates can exhibit certain forms of energy saving mechanisms when ambient temperatures are low and/or food is scarce. Therefore, the aim of the study was to determine...
journal_title:Scientific reports
pub_type: 杂志文章
doi:10.1038/s41598-017-07946-7
更新日期:2017-08-08 00:00:00
abstract::It is a significant challenge to regenerate full-thickness skin defects with sweat glands. Various skin substitutes have been developed to resolve this issue with minimal success. In this study, to yield a novel construct for in situ regeneration of sweat glands, the collagen-chitosan porous scaffold was combined with...
journal_title:Scientific reports
pub_type: 杂志文章
doi:10.1038/s41598-017-17967-x
更新日期:2017-12-15 00:00:00
abstract::Why lightning sometimes has multiple discharges to ground is an unanswered question. Recently, the observation of small plasma structures on positive leaders re-ignited the search. These small plasma structures were observed as pulsing radio sources along the positive leader length and were named "needles". Needles ma...
journal_title:Scientific reports
pub_type: 杂志文章
doi:10.1038/s41598-020-74597-6
更新日期:2020-10-15 00:00:00
abstract::Staphylococcus aureus strains carrying enterotoxin A gene (sea) causes food poisoning and cannot be distinguished from non-pathogenic strains by the culture method. Here, we developed a rapid, specific and sensitive visual detection of sea using loop-mediated isothermal amplification (LAMP) combined with nanogold prob...
journal_title:Scientific reports
pub_type: 杂志文章
doi:10.1038/s41598-020-64710-0
更新日期:2020-05-08 00:00:00
abstract::Silver nanoparticles (AgNPs) demonstrating good antimicrobial activity are widely used in many fields. However, the impact of AgNPs on the community structures of marine biofilms that drive biogeochemical cycling processes and the recruitment of marine invertebrate larvae remains unknown. Here, we employed MiSeq seque...
journal_title:Scientific reports
pub_type: 杂志文章
doi:10.1038/srep37406
更新日期:2016-11-21 00:00:00
abstract::Nitric oxide (NO) is an important signalling molecule involved in haemostasis. NO, present as endogenous S-nitrosothiols, is released by cysteine through a transnitrosation reaction. To exploit this mechanism, cysteine was immobilised onto the different carboxylated polyethylene terephthalate (PET) surfaces using 1-st...
journal_title:Scientific reports
pub_type: 杂志文章
doi:10.1038/s41598-019-53108-2
更新日期:2019-11-13 00:00:00
abstract::An amendment to this paper has been published and can be accessed via a link at the top of the paper. ...
journal_title:Scientific reports
pub_type: 已发布勘误
doi:10.1038/s41598-020-60642-x
更新日期:2020-02-21 00:00:00
abstract::A correction to this article has been published and is linked from the HTML and PDF versions of this paper. The error has been fixed in the paper. ...
journal_title:Scientific reports
pub_type: 已发布勘误
doi:10.1038/s41598-018-22377-8
更新日期:2018-03-06 00:00:00
abstract::Spiny mice of the genus Acomys display several unique physiological traits, including menstruation and scar-free wound healing; characteristics that are exceedingly rare in mammals, and of considerable interest to the scientific community. These unique attributes, and the potential for spiny mice to accurately model h...
journal_title:Scientific reports
pub_type: 杂志文章
doi:10.1038/s41598-017-09334-7
更新日期:2017-08-21 00:00:00
abstract::Thermoelectrics is a challenging issue for modern and future energy conversion and recovery technology. Carbon nanotubes are promising active thermoelectic materials owing to their narrow bandgap energy and high charge carrier mobility, and they can be integrated into flexible thermoelectrics that can recover any wast...
journal_title:Scientific reports
pub_type: 杂志文章
doi:10.1038/srep03344
更新日期:2013-11-26 00:00:00
abstract::The psychrophilic (cold-loving) fungus Pseudogymnoascus destructans was discovered more than a decade ago to be the pathogen responsible for white-nose syndrome, an emerging disease of North American bats causing unprecedented population declines. The same species of fungus is found in Europe but without associated mo...
journal_title:Scientific reports
pub_type: 杂志文章
doi:10.1038/s41598-020-70375-6
更新日期:2020-08-17 00:00:00
abstract::We have quantitatively evaluated the macular and peripapillary microvascular changes in eyes with chiasmal compression caused by brain tumors compared with healthy control eyes using optical coherence tomography angiography (OCT-A) and correlated them with other ocular parameters. This cross-sectional study involved t...
journal_title:Scientific reports
pub_type: 杂志文章
doi:10.1038/s41598-020-59158-1
更新日期:2020-02-07 00:00:00
abstract::Moyamoya disease is an idiopathic human cerebrovascular disorder that is characterized by progressive stenosis and abnormal collateral vessels. We recently identified mysterin/RNF213 as its first susceptibility gene, which encodes a 591-kDa protein containing enzymatically active P-loop ATPase and ubiquitin ligase dom...
journal_title:Scientific reports
pub_type: 杂志文章
doi:10.1038/srep04442
更新日期:2014-03-24 00:00:00
abstract::Lytic transglycosylases (LTs) catalyze the non-hydrolytic cleavage of the bacterial cell wall by an intramolecular transacetalization reaction. This reaction is critically and broadly important in modifications of the bacterial cell wall in the course of its biosynthesis, recycling, manifestation of virulence, inserti...
journal_title:Scientific reports
pub_type: 杂志文章
doi:10.1038/s41598-018-22527-y
更新日期:2018-03-07 00:00:00
abstract::Skeletal muscle represents the largest pool of body zinc, however, little is known about muscle zinc homeostasis or muscle-specific zinc functions. Zip14 (Slc39a14) was the most highly expressed zinc transporter in skeletal muscle of mice in response to LPS-induced inflammation. We compared metabolic parameters of ske...
journal_title:Scientific reports
pub_type: 杂志文章
doi:10.1038/s41598-020-61059-2
更新日期:2020-03-04 00:00:00
abstract::Production of reactive nitrogen species (NO) is a key step in the immune response following infections. NO induces lesions to bacterial DNA, thus limiting bacterial growth within hosts. Using two pathogenic bacteria, Bacillus cereus and Shigella flexneri, we show that the DNA-repair protein Mfd (Mutation-Frequency-Dec...
journal_title:Scientific reports
pub_type: 杂志文章
doi:10.1038/srep29349
更新日期:2016-07-20 00:00:00
abstract::Traditionally, dynamic atomic force microscopy (AFM) techniques are based on the analysis of the quasi-steady state response of the cantilever deflection in terms of Fourier analysis. Here we describe a technique that instead exploits the often disregarded transient response of the cantilever through a relatively mode...
journal_title:Scientific reports
pub_type: 杂志文章
doi:10.1038/s41598-019-49104-1
更新日期:2019-09-03 00:00:00
abstract::In the event of a nuclear attack or large-scale radiation event, there would be an urgent need for assessing the dose to which hundreds or thousands of individuals were exposed. Biodosimetry approaches are being developed to address this need, including transcriptomics. Studies have identified many genes with potentia...
journal_title:Scientific reports
pub_type: 杂志文章
doi:10.1038/s41598-019-54967-5
更新日期:2019-12-05 00:00:00
abstract::Present study aimed to estimate the incidence of recurrent SARS-CoV-2 RNA positivity after recovery from COVID-19 and to determine the factors associated with recurrent positivity. We searched the PubMed, MedRxiv, BioRxiv, the Cochrane Library, ClinicalTrials.gov, and the World Health Organization International Clinic...
journal_title:Scientific reports
pub_type: 杂志文章,meta分析
doi:10.1038/s41598-020-77739-y
更新日期:2020-11-26 00:00:00
abstract::Increased filaggrin expression was found to be correlated with severity scores in chronic spontaneous urticaria (CSU); however, the role of filaggrin breakdown products (FBPs) in CSU has not been studied. We collected stratum corneum (SC) specimens from the volar forearms of 10 CSU patients, 10 AD patients, and 10 hea...
journal_title:Scientific reports
pub_type: 杂志文章
doi:10.1038/s41598-017-01487-9
更新日期:2017-05-02 00:00:00
abstract::Recently, the discovery of room temperature magnetoelectricity in organic charge transfer complexes has reignited interest in the multiferroic field. The solution processed, large-area and low cost organic semiconductor materials offer new possibilities for the functional all organic multiferroic devices. Here we repo...
journal_title:Scientific reports
pub_type: 杂志文章
doi:10.1038/srep28656
更新日期:2016-06-23 00:00:00
abstract::An amendment to this paper has been published and can be accessed via a link at the top of the paper. ...
journal_title:Scientific reports
pub_type: 已发布勘误
doi:10.1038/s41598-020-68522-0
更新日期:2020-07-10 00:00:00
abstract::The jerantinine family of Aspidosperma indole alkaloids from Tabernaemontana corymbosa are potent microtubule-targeting agents with broad spectrum anticancer activity. The natural supply of these precious metabolites has been significantly disrupted due to the inclusion of T. corymbosa on the endangered list of threat...
journal_title:Scientific reports
pub_type: 杂志文章
doi:10.1038/s41598-018-28880-2
更新日期:2018-07-13 00:00:00
abstract::Ulcerative colitis (UC) is a chronic relapsing-remitting form of inflammatory bowel disease (IBD) that increases the risk of colorectal cancer, the third most common malignancy in humans. Oxidative stress is a risk factor for the development of UC. The Keap1-Nrf2-ARE pathway is one of the most important defensive mech...
journal_title:Scientific reports
pub_type: 杂志文章
doi:10.1038/srep26585
更新日期:2016-05-24 00:00:00
abstract::Although microbes influence plant growth, little is known about the impact of microbial diversity on plant fitness trade-offs, intraspecific-interactions, and soil nutrient dynamics in the context of biodiversity-ecosystem functioning (BEF) research. The BEF theory states that higher species richness can enhance ecosy...
journal_title:Scientific reports
pub_type: 杂志文章
doi:10.1038/s41598-020-72516-3
更新日期:2020-09-22 00:00:00
abstract::An amendment to this paper has been published and can be accessed via a link at the top of the paper. ...
journal_title:Scientific reports
pub_type: 已发布勘误
doi:10.1038/s41598-020-67913-7
更新日期:2020-06-30 00:00:00
abstract::Silver has long been employed as an electrically conductive component, and morphology-dependent properties have been actively investigated. Here we present a novel scalable synthesis method of flower-shaped silver nanoparticles (silver nanoflowers, Ag NFs). The preferential affinity of citrate molecules on (111) surfa...
journal_title:Scientific reports
pub_type: 杂志文章
doi:10.1038/srep34894
更新日期:2016-10-07 00:00:00
abstract::Upper mantle viscosity plays a key role in understanding plate tectonics and is usually extrapolated from laboratory-based creep measurements of upper mantle conditions or constrained by modeling geodetic and post-seismic observations. At present, an effective method to obtain a high-resolution viscosity structure is ...
journal_title:Scientific reports
pub_type: 杂志文章
doi:10.1038/s41598-018-20250-2
更新日期:2018-01-29 00:00:00
abstract::The performance and emission affected by piston catalytic coating have been analyzed in this study. The main contributing factors for improving performance such as load, fuel and speed have been preferred to reduce the emission and improve the performance of an IC engine. These specific standard parameters have been m...
journal_title:Scientific reports
pub_type: 杂志文章
doi:10.1038/s41598-019-57129-9
更新日期:2020-02-07 00:00:00