Abstract:
BACKGROUND:The extracellular domain of matrix protein 2 (M2e) of influenza A virus is a promising target for the development of a universal vaccine against influenza because M2e sequences are highly conserved among human influenza A strains. However, native M2e is poorly immunogenic, but its immunogenicity can be increased by delivery in combination with adjuvants or carrier particles. It was previously shown that fusion of M2e to bacterial flagellin, the ligand for Toll-like receptor (TLR) 5 and powerful mucosal adjuvant, significantly increases the immunogenicity and protective capacity of M2e. RESULTS:In this study, we report for the first time the transient expression in plants of a recombinant protein Flg-4M comprising flagellin of Salmonella typhimurium fused to four tandem copies of the M2e peptide. The chimeric construct was expressed in Nicotiana benthamiana plants using either the self-replicating potato virus X (PVX) based vector, pA7248AMV-GFP, or the cowpea mosaic virus (CPMV)-derived expression vector, pEAQ-HT. The highest expression level up to 30% of total soluble protein (about 1 mg/g of fresh leaf tissue) was achieved with the PVX-based expression system. Intranasal immunization of mice with purified Flg-4M protein induced high levels of M2e-specific serum antibodies and provided protection against lethal challenge with influenza virus. CONCLUSIONS:This study confirms the usefulness of flagellin as a carrier of M2e and its relevance for the production of M2e-based candidate influenza vaccines in plants.
journal_name
BMC Biotechnoljournal_title
BMC biotechnologyauthors
Mardanova ES,Kotlyarov RY,Kuprianov VV,Stepanova LA,Tsybalova LM,Lomonosoff GP,Ravin NVdoi
10.1186/s12896-015-0164-6subject
Has Abstractpub_date
2015-05-29 00:00:00pages
42issn
1472-6750pii
10.1186/s12896-015-0164-6journal_volume
15pub_type
杂志文章abstract:BACKGROUND:Xenoantigens are a major source of concern with regard to the success of interspecific xenografts. GGTA1 encodes α1,3-galactosyltransferase, which is essential for the biosynthesis of galactosyl-alpha 1,3-galactose, the major xenoantigen causing hyperacute rejection. GGTA1-modified pigs, therefore, are promi...
journal_title:BMC biotechnology
pub_type: 杂志文章
doi:10.1186/s12896-020-00638-7
更新日期:2020-08-18 00:00:00
abstract:BACKGROUND:The insect cell line is a critical component in the production of recombinant proteins in the baculovirus expression system and new cell lines hold the promise of increasing both quantity and quality of protein production. RESULTS:Seventy cell lines were established by single-cell cloning from a primary cul...
journal_title:BMC biotechnology
pub_type: 杂志文章
doi:10.1186/1472-6750-10-50
更新日期:2010-07-05 00:00:00
abstract:BACKGROUND:Construction of high quality cDNA libraries from the usually low amounts of eukaryotic mRNA extracted from environmental samples is essential in functional metatranscriptomics for the selection of functional, full-length genes encoding proteins of interest. Many of the inserts in libraries constructed by sta...
journal_title:BMC biotechnology
pub_type: 杂志文章
doi:10.1186/1472-6750-14-80
更新日期:2014-09-03 00:00:00
abstract:BACKGROUND:Gene therapy continues to hold great potential for treating many different types of disease and dysfunction. Safe and efficient techniques for gene transfer and expression in vivo are needed to enable gene therapeutic strategies to be effective in patients. Currently, the most commonly used methods employ re...
journal_title:BMC biotechnology
pub_type: 杂志文章
doi:10.1186/1472-6750-11-62
更新日期:2011-06-02 00:00:00
abstract:BACKGROUND:CRISPR/Cas9 is widely used for precise genetic editing in various organisms. CRISPR/Cas9 editing may in many plants be hampered by the presence of complex and high ploidy genomes and inefficient or poorly controlled delivery of the CRISPR/Cas9 components to gamete cells or cells with regenerative potential. ...
journal_title:BMC biotechnology
pub_type: 杂志文章
doi:10.1186/s12896-019-0530-x
更新日期:2019-06-17 00:00:00
abstract:BACKGROUND:Over-expressed native or recombinant proteins are commonly used for industrial and pharmaceutical purposes, as well as for research. Proteins of interest need to be purified in sufficient quantity, quality and specific activity to justify their commercial price and eventual medical use. Proteome quality was ...
journal_title:BMC biotechnology
pub_type: 杂志文章
doi:10.1186/s12896-016-0259-8
更新日期:2016-03-11 00:00:00
abstract:BACKGROUND:Aldehyde-deformylating oxygenase (ADO) is a key enzyme involved in the biosynthetic pathway of fatty alk(a/e)nes in cyanobacteria. However, cADO (cyanobacterial ADO) showed extreme low activity with the k cat value below 1 min-1, which would limit its application in biofuel production. To identify the activi...
journal_title:BMC biotechnology
pub_type: 杂志文章
doi:10.1186/s12896-017-0351-8
更新日期:2017-03-16 00:00:00
abstract:BACKGROUND:Proteins in inclusion bodies (IBs) present native-like secondary structures. However, chaotropic agents at denaturing concentrations, which are widely used for IB solubilization and subsequent refolding, unfold these secondary structures. Removal of the chaotropes frequently causes reaggregation and poor rec...
journal_title:BMC biotechnology
pub_type: 杂志文章
doi:10.1186/s12896-018-0486-2
更新日期:2018-12-12 00:00:00
abstract:BACKGROUND:Fungal laccases are multicopper oxidases with huge applicability in different sectors. Here, we describe the development of a set of high-throughput colorimetric assays for screening laccase libraries in directed evolution studies. RESULTS:Firstly, we designed three colorimetric assays based on the oxidatio...
journal_title:BMC biotechnology
pub_type: 杂志文章
doi:10.1186/1472-6750-13-90
更新日期:2013-10-26 00:00:00
abstract:BACKGROUND:Dengue is a public health problem of global significance for which there is neither an effective antiviral therapy nor a preventive vaccine. It is a mosquito-borne viral disease, caused by dengue (DEN) viruses, which are members of the Flaviviridae family. There are four closely related serotypes, DEN-1, DEN...
journal_title:BMC biotechnology
pub_type: 杂志文章
doi:10.1186/1472-6750-7-10
更新日期:2007-02-15 00:00:00
abstract:BACKGROUND:Nanoparticles appear to be promising devices for application in the agriculture and food industries, but information regarding the response of plants to contact with nano-devices is scarce. Toxic effects may be imposed depending on the type and concentration of nanoparticle as well as time of exposure. A num...
journal_title:BMC biotechnology
pub_type: 杂志文章
doi:10.1186/1472-6750-13-111
更新日期:2013-12-20 00:00:00
abstract:BACKGROUND:Tetanus neurotoxin (TeNT) is taken up at nerve terminals and undergoes retrograde migration. The toxic properties of TeNT reside in the toxin light chain (L), but like complete TeNT, the TeNT heavy chain (TTH) and the C-terminal domain (TTC) alone can bind and enter into neurons. Here, we explored whether at...
journal_title:BMC biotechnology
pub_type: 杂志文章
doi:10.1186/s12896-018-0452-z
更新日期:2018-06-11 00:00:00
abstract:BACKGROUND:In the previous study, the cellulolytic Escherichia coli ZH-4 isolated from bovine rumen was found to show extracellular cellulase activity and could degrade cellulose in the culture. The goal of this work was to identify and characterize the secreted cellulase of E. coli ZH-4. It will be helpful to re-under...
journal_title:BMC biotechnology
pub_type: 杂志文章
doi:10.1186/s12896-019-0556-0
更新日期:2019-08-27 00:00:00
abstract:BACKGROUND:Hydrophobic polystyrene is the most common material for solid phase immunoassay. Proteins are immobilized on polystyrene by passive adsorption, which often causes considerable denaturation. Biological macromolecules were found to better retain their functional activity when immobilized on hydrophilic materia...
journal_title:BMC biotechnology
pub_type: 杂志文章
doi:10.1186/1472-6750-1-4
更新日期:2001-01-01 00:00:00
abstract:BACKGROUND:In a previous study, anthocyanin levels in potato plants were increased by manipulating genes connected with the flavonoid biosynthesis pathway. However, starch content and tuber yield were dramatically reduced in the transgenic plants, which over-expressed dihydroflavonol reductase (DFR). RESULTS:Transgeni...
journal_title:BMC biotechnology
pub_type: 杂志文章
doi:10.1186/1472-6750-8-25
更新日期:2008-03-05 00:00:00
abstract:BACKGROUND:The ketoisovalerate reductase (EC 1.2.7.7 ) is required for the formation of beauvericin via the nonribosomal peptide synthetase biosynthetic pathway. It catalyzes the NADPH-specific reduction of ketoisovaleric acid to hydroxyisovalerate. However, little is known about the bioinformatics' data about the 2-Ki...
journal_title:BMC biotechnology
pub_type: 杂志文章
doi:10.1186/1472-6750-12-55
更新日期:2012-08-23 00:00:00
abstract:BACKGROUND:Plasminogen activator inhibitor type-2 (PAI-2, SERPINB2) is an irreversible, specific inhibitor of the urokinase plasminogen activator (uPA). Since overexpression of uPA at the surface of cancer cells is linked to malignancy, targeting of uPA by exogenous recombinant PAI-2 has been proposed as the basis of p...
journal_title:BMC biotechnology
pub_type: 杂志文章
doi:10.1186/1472-6750-9-43
更新日期:2009-05-14 00:00:00
abstract:BACKGROUND:Urease B is an important virulence factor that is required for Helicobacter pylori to colonise the gastric mucosa. Mouse monoclonal antibodies (mAbs) that inhibit urease B enzymatic activity will be useful as vaccines for the prevention and treatment of H. pylori infection. Here, we produced murine mAbs agai...
journal_title:BMC biotechnology
pub_type: 杂志文章
doi:10.1186/1472-6750-10-84
更新日期:2010-11-30 00:00:00
abstract:BACKGROUND:Microarray technology has been used extensively over the past 10 years for assessing gene expression, and has facilitated precise genetic profiling of everything from tumors to small molecule drugs. By contrast, arraying cell membranes in a manner which preserves their ability to mediate biochemical processe...
journal_title:BMC biotechnology
pub_type: 杂志文章
doi:10.1186/1472-6750-5-18
更新日期:2005-06-16 00:00:00
abstract:BACKGROUND:Cost-effective production of industrially important enzymes is a key for their successful exploitation on industrial scale. Keeping in view the extensive industrial applications of lignin peroxidase (LiP), this study was performed to purify and characterize the LiP from an indigenous strain of Trametes versi...
journal_title:BMC biotechnology
pub_type: 杂志文章
doi:10.1186/1472-6750-12-46
更新日期:2012-08-03 00:00:00
abstract:BACKGROUND:The ubiquitin protein is present in all eukaryotic cells and promoters from ubiquitin genes are good candidates to regulate the constitutive expression of transgenes in plants. Therefore, two switchgrass (Panicum virgatum L.) ubiquitin genes (PvUbi1 and PvUbi2) were cloned and characterized. Reporter constru...
journal_title:BMC biotechnology
pub_type: 杂志文章
doi:10.1186/1472-6750-11-74
更新日期:2011-07-11 00:00:00
abstract:BACKGROUND:Microarrays are powerful tools for DNA-based molecular diagnostics and identification of pathogens. Most target a limited range of organisms and are based on only one or a very few genes for specific identification. Such microarrays are limited to organisms for which specific probes are available, and often ...
journal_title:BMC biotechnology
pub_type: 杂志文章
doi:10.1186/1472-6750-10-13
更新日期:2010-02-17 00:00:00
abstract:BACKGROUND:The proteomics literature has seen a proliferation of publications that seek to apply the rapidly improving technology of 2D gels to study various biological systems. However, there is a dearth of systematic studies that have investigated appropriate statistical approaches to analyse the data from these expe...
journal_title:BMC biotechnology
pub_type: 杂志文章
doi:10.1186/1472-6750-5-7
更新日期:2005-02-11 00:00:00
abstract:BACKGROUND:Cytotoxic drugs are non-selective between normal and pathological tissue, and this poses a challenge regarding the strategy for treatment of tumors. To achieve sufficient antitumor activity for colorectal carcinoma, optimization of the therapeutic regimen is of great importance. We investigated the ability o...
journal_title:BMC biotechnology
pub_type: 杂志文章
doi:10.1186/1472-6750-11-21
更新日期:2011-03-15 00:00:00
abstract:BACKGROUND:There is an imperative necessity for alternative sources of energy able to reduce the world dependence of fossil oil. One of the most successful options is ethanol obtained mainly from sugarcane and corn fermentation. The foremost residue from sugarcane industry is the bagasse, a rich lignocellulosic raw mat...
journal_title:BMC biotechnology
pub_type: 杂志文章
doi:10.1186/1472-6750-13-94
更新日期:2013-10-31 00:00:00
abstract:BACKGROUND:Cell-penetrating peptides (CPPs) have been widely used as carriers to transport different molecules into living cells, whereas messenger RNAs (mRNAs) have been utilized as target molecules for the prevention and treatment of various diseases. However, the instability of CPPs and mRNAs has limited their appli...
journal_title:BMC biotechnology
pub_type: 杂志文章
doi:10.1186/s12896-016-0274-9
更新日期:2016-05-28 00:00:00
abstract:BACKGROUND:Interest in using plants for production of recombinant proteins such as monoclonal antibodies is growing, but proteolytic degradation, leading to a loss of functionality and complications in downstream purification, is still a serious problem. RESULTS:In this study, we investigated the dynamics of the assem...
journal_title:BMC biotechnology
pub_type: 杂志文章
doi:10.1186/1472-6750-11-128
更新日期:2011-12-30 00:00:00
abstract:BACKGROUND:Serine hydroxymethyltransferase (SHMT) is the key enzyme in L-serine enzymatic production, suggesting the importance of obtaining a SHMT with high activity. RESULTS:Here, a novel SHMT gene, glyA, was obtained through degenerate oligonucleotide-primed PCR and encoded a novel SHMT with 54.3% similarity to the...
journal_title:BMC biotechnology
pub_type: 杂志文章
doi:10.1186/s12896-014-0093-9
更新日期:2014-11-14 00:00:00
abstract:BACKGROUND:Chinese hamster ovary (CHO) cells have become the host of choice for the production of recombinant proteins, due to their capacity for correct protein folding, assembly, and posttranslational modifications. The most widely used system for recombinant proteins is the gene amplification procedure that uses the...
journal_title:BMC biotechnology
pub_type: 杂志文章
doi:10.1186/s12896-015-0218-9
更新日期:2015-10-23 00:00:00
abstract:BACKGROUND:Rapid, robust and reversible induction of transgene expression would significantly facilitate cancer gene therapy as well as allow the in vivo functional study of newly discovered genes in tumor formation and progression. The popularity of the ecdysone inducible gene switch system has led us to investigate w...
journal_title:BMC biotechnology
pub_type: 杂志文章
doi:10.1186/1472-6750-1-11
更新日期:2001-01-01 00:00:00