Transcriptome study and identification of potential marker genes related to the stable expression of recombinant proteins in CHO clones.

abstract：BACKGROUND:Aldehyde-deformylating oxygenase (ADO) is a key enzyme involved in the biosynthetic pathway of fatty alk(a/e)nes in cyanobacteria. However, cADO (cyanobacterial ADO) showed extreme low activity with the k cat value below 1 min-1, which would limit its application in biofuel production. To identify the activi...

journal_title：BMC biotechnology

authors： Wang Q,Bao L,Jia C,Li M,Li JJ,Lu X

更新日期：2017-03-16 00:00:00

abstract：BACKGROUND:The therapeutic use of α-amidated peptides (e.g. calcitonin, glucagon-like peptide) has increased dramatically, but there are major impediments to wider use of such peptides. Larger peptides are expensive to synthesize, and short plasma half-lives frequently limit the clinical circumstances in which the pept...

journal_title：BMC biotechnology

authors： Carlson KR,Pomerantz SC,Li J,Vafa O,Naso M,Strohl W,Mains RE,Eipper BA

更新日期：2015-06-27 00:00:00

abstract：BACKGROUND:Fungal laccases are multicopper oxidases with huge applicability in different sectors. Here, we describe the development of a set of high-throughput colorimetric assays for screening laccase libraries in directed evolution studies. RESULTS:Firstly, we designed three colorimetric assays based on the oxidatio...

journal_title：BMC biotechnology

authors： Pardo I,Chanagá X,Vicente AI,Alcalde M,Camarero S

更新日期：2013-10-26 00:00:00

abstract：BACKGROUND:Microarrays are powerful tools for DNA-based molecular diagnostics and identification of pathogens. Most target a limited range of organisms and are based on only one or a very few genes for specific identification. Such microarrays are limited to organisms for which specific probes are available, and often ...

journal_title：BMC biotechnology

authors： Pasquer F,Pelludat C,Duffy B,Frey JE

更新日期：2010-02-17 00:00:00

abstract：BACKGROUND:CRISPR/Cas9 is widely used for precise genetic editing in various organisms. CRISPR/Cas9 editing may in many plants be hampered by the presence of complex and high ploidy genomes and inefficient or poorly controlled delivery of the CRISPR/Cas9 components to gamete cells or cells with regenerative potential. ...

journal_title：BMC biotechnology

authors： Petersen BL,Möller SR,Mravec J,Jørgensen B,Christensen M,Liu Y,Wandall HH,Bennett EP,Yang Z

更新日期：2019-06-17 00:00:00

abstract：BACKGROUND:There is an imperative necessity for alternative sources of energy able to reduce the world dependence of fossil oil. One of the most successful options is ethanol obtained mainly from sugarcane and corn fermentation. The foremost residue from sugarcane industry is the bagasse, a rich lignocellulosic raw mat...

journal_title：BMC biotechnology

authors： Robl D,Delabona Pda S,Mergel CM,Rojas JD,Costa Pdos S,Pimentel IC,Vicente VA,da Cruz Pradella JG,Padilla G

更新日期：2013-10-31 00:00:00

abstract：BACKGROUND:Alkaline phosphatase (AP) catalyzes the hydrolytic cleavage of phosphate monoesters under alkaline conditions and plays important roles in microbial ecology and molecular biology applications. Here, we report on the first isolation and biochemical characterization of a thermolabile AP from a metagenome. RES...

journal_title：BMC biotechnology

authors： Lee DH,Choi SL,Rha E,Kim SJ,Yeom SJ,Moon JH,Lee SG

更新日期：2015-01-31 00:00:00

abstract：BACKGROUND:Methylated resveratrol analogs show similar biological activities that are comparable with those of the resveratrol. However, the methylated resveratrol analogs exhibit better bioavailability as they are more easily transported into the cell and more resistant to degradation. Although these compounds are wid...

journal_title：BMC biotechnology

authors： Kang SY,Lee JK,Choi O,Kim CY,Jang JH,Hwang BY,Hong YS

更新日期：2014-07-17 00:00:00

abstract：BACKGROUND:Serine hydroxymethyltransferase (SHMT) is the key enzyme in L-serine enzymatic production, suggesting the importance of obtaining a SHMT with high activity. RESULTS:Here, a novel SHMT gene, glyA, was obtained through degenerate oligonucleotide-primed PCR and encoded a novel SHMT with 54.3% similarity to the...

journal_title：BMC biotechnology

authors： Jiang W,Chen L,Hu N,Yuan S,Li B,Liu Z

更新日期：2014-11-14 00:00:00

abstract：BACKGROUND:MicroRNAs are important regulators of gene expression at the post-transcriptional level and play an important role in many biological processes. Due to the important biological role it is of great interest to quantitatively determine their expression level in different biological settings. RESULTS:We descri...

journal_title：BMC biotechnology

authors： Balcells I,Cirera S,Busk PK

更新日期：2011-06-25 00:00:00

abstract：BACKGROUND:Smoking and tobacco use continue to be the largest preventable causes of death globally. A novel therapeutic approach has recently been proposed: administration of an enzyme that degrades nicotine, the main addictive component of tobacco, minimizing brain exposure and reducing its reinforcing effects. Pre-cl...

journal_title：BMC biotechnology

authors： Thisted T,Biesova Z,Walmacq C,Stone E,Rodnick-Smith M,Ahmed SS,Horrigan SK,Van Engelen B,Reed C,Kalnik MW

更新日期：2019-08-02 00:00:00

abstract：BACKGROUND:Acetyl-xylan esterase (AXE, EC 3.1.1.72) hydrolyses acetate group from the linear chain of xylopyranose residues bound by β-1,4-linkage. The enzyme finds commercial applications in bio-bleaching of wood pulp, treating animal feed to increase digestibility, processing food to increase clarification and conver...

journal_title：BMC biotechnology

authors： Atta S,Ali S,Akhtar MN,Haq I

更新日期：2011-05-16 00:00:00

abstract：BACKGROUND:The ability to produce the same recombinant protein in both prokaryotic and eukaryotic cells offers many experimental opportunities. However, the cloning of the same gene into multiple plasmids is required, which is time consuming, laborious and still may not produce soluble, stable protein in sufficient qua...

journal_title：BMC biotechnology

authors： Sinah N,Williams CA,Piper RC,Shields SB

更新日期：2012-08-23 00:00:00

abstract：BACKGROUND:Interest in using plants for production of recombinant proteins such as monoclonal antibodies is growing, but proteolytic degradation, leading to a loss of functionality and complications in downstream purification, is still a serious problem. RESULTS:In this study, we investigated the dynamics of the assem...

journal_title：BMC biotechnology

authors： Hehle VK,Paul MJ,Drake PM,Ma JK,van Dolleweerd CJ

更新日期：2011-12-30 00:00:00

abstract：BACKGROUND:Plants are increasingly being examined as alternative recombinant protein expression systems. Recombinant protein expression levels in plants from Tobacco mosaic virus (TMV)-based vectors are much higher than those possible from plant promoters. However the common TMV expression vectors are costly, and at ti...

journal_title：BMC biotechnology

authors： Lindbo JA

更新日期：2007-08-27 00:00:00

abstract：BACKGROUND:The extracellular domain of matrix protein 2 (M2e) of influenza A virus is a promising target for the development of a universal vaccine against influenza because M2e sequences are highly conserved among human influenza A strains. However, native M2e is poorly immunogenic, but its immunogenicity can be incre...

journal_title：BMC biotechnology

authors： Mardanova ES,Kotlyarov RY,Kuprianov VV,Stepanova LA,Tsybalova LM,Lomonosoff GP,Ravin NV

更新日期：2015-05-29 00:00:00

abstract：BACKGROUND:Asian rust (Phakopsora pachyrhizi) is a common disease in Brazilian soybean fields and it is difficult to control. To identify a biochemical candidate with potential to combat this disease, a new chitinase-like xylanase inhibitor protein (XIP) from coffee (Coffea arabica) (CaclXIP) leaves was cloned into the...

journal_title：BMC biotechnology

authors： Vasconcelos EA,Santana CG,Godoy CV,Seixas CD,Silva MS,Moreira LR,Oliveira-Neto OB,Price D,Fitches E,Filho EX,Mehta A,Gatehouse JA,Grossi-De-Sa MF

更新日期：2011-02-07 00:00:00

abstract：BACKGROUND:Polysialic acid (polySia) is a carbohydrate modification of the neural cell adhesion molecule (NCAM), which is implicated in neural differentiation and plays an important role in tumor development and metastasis. Polysialylation of NCAM is mediated by two Golgi-resident polysialyltransferases (polyST) ST8Sia...

journal_title：BMC biotechnology

authors： Somplatzki S,Mühlenhoff M,Kröger A,Gerardy-Schahn R,Böldicke T

更新日期：2017-05-12 00:00:00

abstract：BACKGROUND:microRNAs (miRNAs) are short non-coding RNAs that fine-tune gene expression. The aberrant expression of miRNAs is associated with many diseases and they have both therapeutic and biomarker potential. However, our understanding of their usefulness is dependent on the tools we have to study them. Previous stud...

journal_title：BMC biotechnology

authors： Brown RAM,Epis MR,Horsham JL,Kabir TD,Richardson KL,Leedman PJ

更新日期：2018-03-16 00:00:00

abstract：BACKGROUND:Polymerase chain reaction (PCR) is one of the most important developments in modern biotechnology. However, PCR is known to introduce biases, especially during multiplex reactions. Recent studies have implicated the DNA polymerase as the primary source of bias, particularly initiation of polymerization on th...

journal_title：BMC biotechnology

authors： Pan W,Byrne-Steele M,Wang C,Lu S,Clemmons S,Zahorchak RJ,Han J

更新日期：2014-01-30 00:00:00

abstract：BACKGROUND:von Willebrand factor (VWF) is a key load bearing domain for mamalian cell adhesion by binding various macromolecular ligands in extracellular matrix such as, collagens, elastin, and glycosaminoglycans. Interestingly, vWF like domains are also commonly found in load bearing systems of marine organisms such a...

journal_title：BMC biotechnology

authors： Yoo HY,Song YH,Foo M,Seo E,Hwang DS,Seo JH

更新日期：2016-02-16 00:00:00

abstract：BACKGROUND:Recombinant factor VIII (FVIII), used for haemophilia A therapy, is one of the most challenging among the therapeutic proteins produced in heterologous expression systems. Deletion variant of FVIII, in which the entire domain B is replaced by a short linker peptide, was approved for medical use. Efficacy and...

journal_title：BMC biotechnology

authors： Orlova NA,Kovnir SV,Gabibov AG,Vorobiev II

更新日期：2017-03-24 00:00:00

abstract：BACKGROUND:The safety of mutagenized and genetically transformed plants remains a subject of scrutiny. Data gathered and communicated on the phenotypic and molecular variation induced by gene transfer technologies will provide a scientific-based means to rationally address such concerns. In this study, genomic structur...

journal_title：BMC biotechnology

authors： Anderson JE,Michno JM,Kono TJ,Stec AO,Campbell BW,Curtin SJ,Stupar RM

更新日期：2016-05-12 00:00:00

abstract：BACKGROUND:The photorespiratory nitrogen cycle in C₃ plants involves an extensive diversion of carbon and nitrogen away from the direct pathways of assimilation. The liberated ammonia is re-assimilated, but up to 25% of the carbon may be released into the atmosphere as CO₂. Because of the loss of CO₂ and high energy co...

journal_title：BMC biotechnology

authors： Carvalho Jde F,Madgwick PJ,Powers SJ,Keys AJ,Lea PJ,Parry MA

更新日期：2011-11-21 00:00:00

abstract：BACKGROUND:The proteomics literature has seen a proliferation of publications that seek to apply the rapidly improving technology of 2D gels to study various biological systems. However, there is a dearth of systematic studies that have investigated appropriate statistical approaches to analyse the data from these expe...

journal_title：BMC biotechnology

authors： Meleth S,Deshane J,Kim H

更新日期：2005-02-11 00:00:00

abstract：BACKGROUND:In the previous study, the cellulolytic Escherichia coli ZH-4 isolated from bovine rumen was found to show extracellular cellulase activity and could degrade cellulose in the culture. The goal of this work was to identify and characterize the secreted cellulase of E. coli ZH-4. It will be helpful to re-under...

journal_title：BMC biotechnology

authors： Pang J,Wang J,Liu Z,Zhang Q,Qi Q

更新日期：2019-08-27 00:00:00

abstract：BACKGROUND:Oleaginous yeast species are an alternative for the production of lipids or triacylglycerides (TAGs). These yeasts are usually non-pathogenic and able to store TAGs ranging from 20 % to 70 % of their cell mass depending on culture conditions. TAGs originating from oleaginous yeasts can be used as the so-call...

journal_title：BMC biotechnology

authors： Lamers D,van Biezen N,Martens D,Peters L,van de Zilver E,Jacobs-van Dreumel N,Wijffels RH,Lokman C

更新日期：2016-05-27 00:00:00

abstract：BACKGROUND:Insect resistance in crops represents a main challenge for agriculture. Transgenic approaches based on proteins displaying insect resistance properties are widely used as efficient breeding strategies. To extend the spectrum of targeted pathogens and overtake the development of resistance, molecular evolutio...

journal_title：BMC biotechnology

authors： Prado GS,Bamogo PKA,de Abreu JAC,Gillet FX,Dos Santos VO,Silva MCM,Brizard JP,Bemquerer MP,Bangratz M,Brugidou C,Sérémé D,Grossi-de-Sa MF,Lacombe S

更新日期：2019-03-08 00:00:00

abstract：: ...

journal_title：BMC biotechnology

authors： Devilder MC,Moyon M,Gautreau-Rolland L,Navet B,Perroteau J,Delbos F,Gesnel MC,Breathnach R,Saulquin X

更新日期：2019-02-18 00:00:00

abstract：BACKGROUND:Despite positive reports on the efficacy of stem cell therapy for the treatment of cardiovascular disease, the nature of stem cell homing to ischemic tissues remains elusive. RESULTS:We used a mouse model of peripheral tissue ischemia to study the survival and homing capacity of dual reporter gene (eGFP/Luc...

journal_title：BMC biotechnology

authors： Everaert BR,Bergwerf I,De Vocht N,Ponsaerts P,Van Der Linden A,Timmermans JP,Vrints CJ

更新日期：2012-12-03 00:00:00