Abstract:
BACKGROUND:Hydrophobic polystyrene is the most common material for solid phase immunoassay. Proteins are immobilized on polystyrene by passive adsorption, which often causes considerable denaturation. Biological macromolecules were found to better retain their functional activity when immobilized on hydrophilic materials. Polyacrylamide is a common material for solid-phase carriers of biological macromolecules, including immunoreagents used in affinity chromatography. New macroformats for immunoassay modified with activated polyacrylamide derivatives seem to be promising. RESULTS:New polymeric matrices for immunoassay in the form of 0.63-cm balls which contain hydrazide functional groups on hydrophilic polymer spacer arms at their surface shell are synthesized by modification of aldehyde-containing polystyrene balls with hydrazide derivatives of poly(meth)acrylic acid. The beads contain up to 0.31 micromol/cm2 active hydrazide groups accessible for covalent reaction with periodate-oxidized antibodies. The matrices obtained allow carrying out the oriented antibody immobilization, which increases the functional activity of immunosorbents. CONCLUSIONS:An efficient site-directed antibody immobilization on a macrosupport is realized. The polymer hydrophilic spacer arms are the most convenient and effective tools for oriented antibody coupling with molded materials. The suggested scheme can be used for the modification of any other solid supports containing electrophilic groups reacting with hydrazides.
journal_name
BMC Biotechnoljournal_title
BMC biotechnologyauthors
Shmanai VV,Nikolayeva TA,Vinokurova LG,Litoshka AAdoi
10.1186/1472-6750-1-4keywords:
subject
Has Abstractpub_date
2001-01-01 00:00:00pages
4issn
1472-6750journal_volume
1pub_type
杂志文章abstract:BACKGROUND:microRNAs (miRNAs) are short non-coding RNAs that fine-tune gene expression. The aberrant expression of miRNAs is associated with many diseases and they have both therapeutic and biomarker potential. However, our understanding of their usefulness is dependent on the tools we have to study them. Previous stud...
journal_title:BMC biotechnology
pub_type: 杂志文章
doi:10.1186/s12896-018-0421-6
更新日期:2018-03-16 00:00:00
abstract:BACKGROUND:Dengue is a public health problem of global significance for which there is neither an effective antiviral therapy nor a preventive vaccine. It is a mosquito-borne viral disease, caused by dengue (DEN) viruses, which are members of the Flaviviridae family. There are four closely related serotypes, DEN-1, DEN...
journal_title:BMC biotechnology
pub_type: 杂志文章
doi:10.1186/1472-6750-7-10
更新日期:2007-02-15 00:00:00
abstract:BACKGROUND:The routine generation of transgenic plants involves analysis of transgene integration into the host genome by means of Southern blotting. However, this technique cannot distinguish between uniformly transformed tissues and the presence of a mixture of transgenic and non-transgenic cells in the same tissue. ...
journal_title:BMC biotechnology
pub_type: 杂志文章
doi:10.1186/1472-6750-10-53
更新日期:2010-07-16 00:00:00
abstract:BACKGROUND:Clustered Regularly Interspaced Short Palindromic Repeats/CRISPR associated protein 9 (CRISPR/Cas9) has been wildly used to generate gene knockout models through inducing indels causing frame-shift. However, there are few studies concerning the post-transcript effects caused by CRISPR-mediated genome editing...
journal_title:BMC biotechnology
pub_type: 杂志文章
doi:10.1186/s12896-018-0472-8
更新日期:2018-10-03 00:00:00
abstract:BACKGROUND:Insect resistance in crops represents a main challenge for agriculture. Transgenic approaches based on proteins displaying insect resistance properties are widely used as efficient breeding strategies. To extend the spectrum of targeted pathogens and overtake the development of resistance, molecular evolutio...
journal_title:BMC biotechnology
pub_type: 杂志文章
doi:10.1186/s12896-019-0507-9
更新日期:2019-03-08 00:00:00
abstract:BACKGROUND:In the search for generic expression strategies for mammalian protein families several bacterial expression vectors were examined for their ability to promote high yields of soluble protein. Proteins studied included cell surface receptors (Ephrins and Eph receptors, CD44), kinases (EGFR-cytoplasmic domain, ...
journal_title:BMC biotechnology
pub_type: 杂志文章
doi:10.1186/1472-6750-4-32
更新日期:2004-12-14 00:00:00
abstract:BACKGROUND:In the previous study, the cellulolytic Escherichia coli ZH-4 isolated from bovine rumen was found to show extracellular cellulase activity and could degrade cellulose in the culture. The goal of this work was to identify and characterize the secreted cellulase of E. coli ZH-4. It will be helpful to re-under...
journal_title:BMC biotechnology
pub_type: 杂志文章
doi:10.1186/s12896-019-0556-0
更新日期:2019-08-27 00:00:00
abstract:BACKGROUND:High recombinant protein productivity in mammalian cell lines is often associated with phenotypic changes in protein content, energy metabolism, and cell growth, but the key determinants that regulate productivity are still not clearly understood. The mammalian target of rapamycin (mTOR) signalling pathway h...
journal_title:BMC biotechnology
pub_type: 杂志文章
doi:10.1186/1472-6750-14-15
更新日期:2014-02-17 00:00:00
abstract:BACKGROUND:Tetanus neurotoxin (TeNT) is taken up at nerve terminals and undergoes retrograde migration. The toxic properties of TeNT reside in the toxin light chain (L), but like complete TeNT, the TeNT heavy chain (TTH) and the C-terminal domain (TTC) alone can bind and enter into neurons. Here, we explored whether at...
journal_title:BMC biotechnology
pub_type: 杂志文章
doi:10.1186/s12896-018-0452-z
更新日期:2018-06-11 00:00:00
abstract:BACKGROUND:Ligand-targeted approaches have proven successful in improving the therapeutic index of a number of drugs. We hypothesized that the specific targeting of TNF-alpha antagonists to inflamed tissues could increase drug efficacy and reduce side effects. RESULTS:Using uteroglobin (UG), a potent anti-inflammatory...
journal_title:BMC biotechnology
pub_type: 杂志文章
doi:10.1186/1472-6750-11-104
更新日期:2011-11-10 00:00:00
abstract:BACKGROUND:New high-throughput sequencing technologies promise a very sensitive and high-resolution analysis of DNA methylation patterns in quantitative terms. However, a detailed and comprehensive comparison with existing validated DNA methylation analysis methods is not yet available. Therefore, a systematic cross-va...
journal_title:BMC biotechnology
pub_type: 杂志文章
doi:10.1186/1472-6750-11-6
更新日期:2011-01-14 00:00:00
abstract:BACKGROUND:Asian rust (Phakopsora pachyrhizi) is a common disease in Brazilian soybean fields and it is difficult to control. To identify a biochemical candidate with potential to combat this disease, a new chitinase-like xylanase inhibitor protein (XIP) from coffee (Coffea arabica) (CaclXIP) leaves was cloned into the...
journal_title:BMC biotechnology
pub_type: 杂志文章
doi:10.1186/1472-6750-11-14
更新日期:2011-02-07 00:00:00
abstract:BACKGROUND:Fungal laccases are multicopper oxidases with huge applicability in different sectors. Here, we describe the development of a set of high-throughput colorimetric assays for screening laccase libraries in directed evolution studies. RESULTS:Firstly, we designed three colorimetric assays based on the oxidatio...
journal_title:BMC biotechnology
pub_type: 杂志文章
doi:10.1186/1472-6750-13-90
更新日期:2013-10-26 00:00:00
abstract:BACKGROUND:To deal with the increasingly severe energy crisis and environmental consequences, biofuels and biochemicals generated from renewable resources could serve as a promising alternative for replacing petroleum as a source of fuel and chemicals, among which isoprene (2-methyl-1,3-butadiene) in particular is of g...
journal_title:BMC biotechnology
pub_type: 杂志文章
doi:10.1186/s12896-016-0236-2
更新日期:2016-01-20 00:00:00
abstract:BACKGROUND:The CRISPR-Cas9 system is a powerful and versatile tool for crop genome editing. However, achieving highly efficient and specific editing in polyploid species can be a challenge. The efficiency and specificity of the CRISPR-Cas9 system depends critically on the gRNA used. Here, we assessed the activities and...
journal_title:BMC biotechnology
pub_type: 杂志文章
doi:10.1186/s12896-019-0565-z
更新日期:2019-10-30 00:00:00
abstract:BACKGROUND:Cell-penetrating peptides (CPPs) have been widely used as carriers to transport different molecules into living cells, whereas messenger RNAs (mRNAs) have been utilized as target molecules for the prevention and treatment of various diseases. However, the instability of CPPs and mRNAs has limited their appli...
journal_title:BMC biotechnology
pub_type: 杂志文章
doi:10.1186/s12896-016-0274-9
更新日期:2016-05-28 00:00:00
abstract:BACKGROUND:Nanoparticles appear to be promising devices for application in the agriculture and food industries, but information regarding the response of plants to contact with nano-devices is scarce. Toxic effects may be imposed depending on the type and concentration of nanoparticle as well as time of exposure. A num...
journal_title:BMC biotechnology
pub_type: 杂志文章
doi:10.1186/1472-6750-13-111
更新日期:2013-12-20 00:00:00
abstract:BACKGROUND:Arachidonic acid (ARA, C20:4, n-6), which belongs to the omega-6 series of polyunsaturated fatty acids and has a variety of biological activities, is commercially produced in Mortierella alpina. Dissolved oxygen or oxygen utilization efficiency is a critical factor for Mortierella alpina growth and arachidon...
journal_title:BMC biotechnology
pub_type: 杂志文章
doi:10.1186/s12896-017-0388-8
更新日期:2017-08-30 00:00:00
abstract:BACKGROUND:With the increasing number of GMOs on the global market the maintenance of European GMO regulations is becoming more complex. For the analysis of a single food or feed sample it is necessary to assess the sample for the presence of many GMO-targets simultaneously at a sensitive level. Several methods have be...
journal_title:BMC biotechnology
pub_type: 杂志文章
doi:10.1186/1472-6750-12-4
更新日期:2012-01-19 00:00:00
abstract:BACKGROUND:Dissolved oxygen tension (DOT) is hardly constant and homogenously distributed in a bioreactor, which can have a negative impact in the metabolism and product synthesis. However, the effects of DOT on plasmid DNA (pDNA) production and quality have not been thoroughly investigated. In the present study, the e...
journal_title:BMC biotechnology
pub_type: 杂志文章
doi:10.1186/s12896-017-0378-x
更新日期:2017-07-04 00:00:00
abstract:BACKGROUND:One of the most commonly used vectors for gene therapy is the adenoviral vector; its ability to tightly regulate transgene expression is critical for optimizing therapeutic outcomes. The tetracycline-regulated system (especially the Tet-On system) for gene expression is one of the most valuable tools for con...
journal_title:BMC biotechnology
pub_type: 杂志文章
doi:10.1186/s12896-015-0121-4
更新日期:2015-02-13 00:00:00
abstract:BACKGROUND:Plants may represent excellent alternatives to classical heterologous protein expression systems, especially for the production of biopharmaceuticals and vaccine components. Modern vaccines are becoming increasingly complex, with the incorporation of multiple antigens. Approaches towards developing an HIV va...
journal_title:BMC biotechnology
pub_type: 杂志文章
doi:10.1186/1472-6750-7-12
更新日期:2007-02-26 00:00:00
abstract:BACKGROUND:Proteins in inclusion bodies (IBs) present native-like secondary structures. However, chaotropic agents at denaturing concentrations, which are widely used for IB solubilization and subsequent refolding, unfold these secondary structures. Removal of the chaotropes frequently causes reaggregation and poor rec...
journal_title:BMC biotechnology
pub_type: 杂志文章
doi:10.1186/s12896-018-0486-2
更新日期:2018-12-12 00:00:00
abstract:BACKGROUND:Eleven new human polyomaviruses (HPyVs) have been identified in the last decade. Serological studies show that these novel HPyVs sub-clinically infect humans at an early age. The routes of infection, entry pathways, and cell tropism of new HPyVs remain unknown. VP1 proteins of polyomaviruses can assembly int...
journal_title:BMC biotechnology
pub_type: 杂志文章
doi:10.1186/s12896-015-0187-z
更新日期:2015-08-04 00:00:00
abstract:BACKGROUND:The identification of known mutations in a cell population is important for clinical applications and basic cancer research. In this work an immobilized form of the polymerase chain reaction, referred to as polony technology, was used to detect mutations as well as gene deletions, resulting in loss of hetero...
journal_title:BMC biotechnology
pub_type: 杂志文章
doi:10.1186/1472-6750-3-11
更新日期:2003-07-23 00:00:00
abstract:BACKGROUND:Analogues of vitamin D3 are extensively used in the treatment of various illnesses, such as osteoporosis, inflammatory skin diseases, and cancer. Functional testing of new vitamin D3 analogues and formulations for improved systemic and topical administration is supported by sensitive screening methods that a...
journal_title:BMC biotechnology
pub_type: 杂志文章
doi:10.1186/1472-6750-11-33
更新日期:2011-04-07 00:00:00
abstract:BACKGROUND:Ultrasound and microbubbles (USMB) have been shown to enhance the intracellular uptake of molecules, generally thought to occur as a result of sonoporation. The underlying mechanism associated with USMB-enhanced intracellular uptake such as membrane disruption and endocytosis may also be associated with USMB...
journal_title:BMC biotechnology
pub_type: 杂志文章
doi:10.1186/s12896-017-0364-3
更新日期:2017-05-18 00:00:00
abstract:BACKGROUND:Alkaline phosphatase (AP) catalyzes the hydrolytic cleavage of phosphate monoesters under alkaline conditions and plays important roles in microbial ecology and molecular biology applications. Here, we report on the first isolation and biochemical characterization of a thermolabile AP from a metagenome. RES...
journal_title:BMC biotechnology
pub_type: 杂志文章
doi:10.1186/s12896-015-0115-2
更新日期:2015-01-31 00:00:00
abstract:BACKGROUND:Iron is an essential element in several pathways of microbial metabolism, and therefore low iron toxicity is expected on the usage of Fe nanoparticles (NPs). This study aims to determine the effect of Fe NPs on biosurfactant production by marine actinobacterium Nocardiopsis sp. MSA13A under solid state cultu...
journal_title:BMC biotechnology
pub_type: 杂志文章
doi:10.1186/1472-6750-14-48
更新日期:2014-05-21 00:00:00
abstract:BACKGROUND:Loop mediated isothermal amplification of nucleic acid templates is a rapid, sensitive and specific method suitable for molecular diagnostics. However the complexity of primer design and the number of primers involved can lead to false positives from non-specific primer interactions. Standard methods of LAMP...
journal_title:BMC biotechnology
pub_type: 杂志文章
doi:10.1186/s12896-019-0549-z
更新日期:2019-08-01 00:00:00