当前位置： SCI文献检索 > BMC BIOTECHNOLOGY期刊下所有文献 > Cloning and characterization of a novel 2-ketoisovalerate reductase from the beauvericin producer Fusarium proliferatum LF061.

Cloning and characterization of a novel 2-ketoisovalerate reductase from the beauvericin producer Fusarium proliferatum LF061.

Abstract：

BACKGROUND:The ketoisovalerate reductase (EC 1.2.7.7 ) is required for the formation of beauvericin via the nonribosomal peptide synthetase biosynthetic pathway. It catalyzes the NADPH-specific reduction of ketoisovaleric acid to hydroxyisovalerate. However, little is known about the bioinformatics' data about the 2-Kiv reductase in Fusarium. To date, heterologous production of the gene KivRFp from Fusarium has not been achieved. RESULTS:The KivRFp gene was subcloned and expressed in Escherichia coli BL21 using the pET expression system. The gene KivRFp contained a 1,359 bp open reading frame (ORF) encoding a polypeptide of 452 amino acids with a molecular mass of 52 kDa. Sequence analysis indicated that it showed 61% and 52% amino acid identities to ketoisovalerate reductase from Beauveria bassiana ATCC 7159 (ACI30654) and Metarhizium acridum CQMa 102 (EFY89891), respectively; and several conserved regions were identified, including the putative nucleotide-binding signature site, GXGXXG, a catalytic triad (Glu405, Asn184, and Lys285). The KivRFp exhibited the highest activity at 35°C and pH 7.5 respectively, by reduction of ketoisovalerate. It also exhibited the high level of stability over wide temperature and pH spectra and in the presence of metal ions or detergents. CONCLUSIONS:A new ketoisovalerate reductase KivRFp was identified and characterized from the depsipeptide-producing fungus F. proliferatum. KivRFp has been shown to have useful properties, such as moderate thermal stability and broad pH optima, and may serve as the starting points for future protein engineering and directed evolution, towards the goal of developing efficient enzyme for downstream biotechnological applications.

journal_name

BMC Biotechnol

journal_title

BMC biotechnology

authors

Zhang T,Jia X,Zhuo Y,Liu M,Gao H,Liu J,Zhang L

doi

10.1186/1472-6750-12-55

subject

Has Abstract

pub_date

2012-08-23 00:00:00

pages

55

issn

1472-6750

pii

1472-6750-12-55

journal_volume

12

pub_type

杂志文章

相关文献

BMC BIOTECHNOLOGY文献大全
  • Nicotiana benthamiana is a suitable transient system for high-level expression of an active inhibitor of cotton boll weevil α-amylase.

    abstract：BACKGROUND:Insect resistance in crops represents a main challenge for agriculture. Transgenic approaches based on proteins displaying insect resistance properties are widely used as efficient breeding strategies. To extend the spectrum of targeted pathogens and overtake the development of resistance, molecular evolutio...

    journal_title：BMC biotechnology

    pub_type： 杂志文章

    doi：10.1186/s12896-019-0507-9

    authors： Prado GS,Bamogo PKA,de Abreu JAC,Gillet FX,Dos Santos VO,Silva MCM,Brizard JP,Bemquerer MP,Bangratz M,Brugidou C,Sérémé D,Grossi-de-Sa MF,Lacombe S

    更新日期：2019-03-08 00:00:00

  • Enhancing the thermostability of α-L-rhamnosidase from Aspergillus terreus and the enzymatic conversion of rutin to isoquercitrin by adding sorbitol.

    abstract：BACKGROUND:Thermally stable α-L-rhamnosidase with cleaving terminal α-L-rhamnose activity has great potential in industrial application. Therefore, it is necessary to find a proper method to improve the thermal stability of α-L-rhamnosidase. RESULTS:In this study, addition of sorbitol has been found to increase the th...

    journal_title：BMC biotechnology

    pub_type： 杂志文章

    doi：10.1186/s12896-017-0342-9

    authors： Ge L,Chen A,Pei J,Zhao L,Fang X,Ding G,Wang Z,Xiao W,Tang F

    更新日期：2017-02-27 00:00:00

  • Multi-substrate chromosome preparations for high throughput comparative FISH.

    abstract：BACKGROUND:A modification of a standard method of fluorescence in situ hybridisation (FISH) is described, by which a combination of several substrates and probes on single microscope slides enables more accurate comparisons of the distribution and abundance of chromosomal sequences and improves the relatively low throu...

    journal_title：BMC biotechnology

    pub_type： 杂志文章

    doi：10.1186/1472-6750-6-20

    authors： Hasterok R,Dulawa J,Jenkins G,Leggett M,Langdon T

    更新日期：2006-03-20 00:00:00

  • Lack of specificity associated with using molecular beacons in loop mediated amplification assays.

    abstract：BACKGROUND:Loop mediated isothermal amplification of nucleic acid templates is a rapid, sensitive and specific method suitable for molecular diagnostics. However the complexity of primer design and the number of primers involved can lead to false positives from non-specific primer interactions. Standard methods of LAMP...

    journal_title：BMC biotechnology

    pub_type： 杂志文章

    doi：10.1186/s12896-019-0549-z

    authors： Hardinge P,Murray JAH

    更新日期：2019-08-01 00:00:00

  • Novel anti-CD38 humanized mAb SG003 possessed enhanced cytotoxicity in lymphoma than Daratumumab via antibody-dependent cell-mediated cytotoxicity.

    abstract：BACKGROUND:In vivo use of monoclonal antibodies has become routine clinical practice in the treatment of human cancer. CD38 is an attractive target, because it has double roles, as a receptor and an ectoenzyme. Daratumumab, an anti-CD38 antibody, is currently in the clinical trials for multiple myeloma. RESULTS:Here w...

    journal_title：BMC biotechnology

    pub_type： 杂志文章

    doi：10.1186/s12896-019-0524-8

    authors： Yu T,Qiao C,Lv M,Tang L

    更新日期：2019-05-22 00:00:00

  • Selective targeted delivery of the TNF-alpha receptor p75 and uteroglobin to the vasculature of inflamed tissues: a preliminary report.

    abstract：BACKGROUND:Ligand-targeted approaches have proven successful in improving the therapeutic index of a number of drugs. We hypothesized that the specific targeting of TNF-alpha antagonists to inflamed tissues could increase drug efficacy and reduce side effects. RESULTS:Using uteroglobin (UG), a potent anti-inflammatory...

    journal_title：BMC biotechnology

    pub_type： 杂志文章

    doi：10.1186/1472-6750-11-104

    authors： Ventura E,Balza E,Borsi L,Tutolo G,Carnemolla B,Castellani P,Zardi L

    更新日期：2011-11-10 00:00:00

  • Using quantitative real-time PCR to detect chimeras in transgenic tobacco and apricot and to monitor their dissociation.

    abstract：BACKGROUND:The routine generation of transgenic plants involves analysis of transgene integration into the host genome by means of Southern blotting. However, this technique cannot distinguish between uniformly transformed tissues and the presence of a mixture of transgenic and non-transgenic cells in the same tissue. ...

    journal_title：BMC biotechnology

    pub_type： 杂志文章

    doi：10.1186/1472-6750-10-53

    authors： Faize M,Faize L,Burgos L

    更新日期：2010-07-16 00:00:00

  • Association of high pressure and alkaline condition for solubilization of inclusion bodies and refolding of the NS1 protein from zika virus.

    abstract：BACKGROUND:Proteins in inclusion bodies (IBs) present native-like secondary structures. However, chaotropic agents at denaturing concentrations, which are widely used for IB solubilization and subsequent refolding, unfold these secondary structures. Removal of the chaotropes frequently causes reaggregation and poor rec...

    journal_title：BMC biotechnology

    pub_type： 杂志文章

    doi：10.1186/s12896-018-0486-2

    authors： Rosa da Silva CM,Chura-Chambi RM,Ramos Pereira L,Cordeiro Y,de Souza Ferreira LC,Morganti L

    更新日期：2018-12-12 00:00:00

  • Secretion of Fc-amidated peptide fusion proteins by Chinese hamster ovary cells.

    abstract：BACKGROUND:The therapeutic use of α-amidated peptides (e.g. calcitonin, glucagon-like peptide) has increased dramatically, but there are major impediments to wider use of such peptides. Larger peptides are expensive to synthesize, and short plasma half-lives frequently limit the clinical circumstances in which the pept...

    journal_title：BMC biotechnology

    pub_type： 杂志文章

    doi：10.1186/s12896-015-0173-5

    authors： Carlson KR,Pomerantz SC,Li J,Vafa O,Naso M,Strohl W,Mains RE,Eipper BA

    更新日期：2015-06-27 00:00:00

  • Optimization of a nicotine degrading enzyme for potential use in treatment of nicotine addiction.

    abstract：BACKGROUND:Smoking and tobacco use continue to be the largest preventable causes of death globally. A novel therapeutic approach has recently been proposed: administration of an enzyme that degrades nicotine, the main addictive component of tobacco, minimizing brain exposure and reducing its reinforcing effects. Pre-cl...

    journal_title：BMC biotechnology

    pub_type： 杂志文章

    doi：10.1186/s12896-019-0551-5

    authors： Thisted T,Biesova Z,Walmacq C,Stone E,Rodnick-Smith M,Ahmed SS,Horrigan SK,Van Engelen B,Reed C,Kalnik MW

    更新日期：2019-08-02 00:00:00

  • Characterizing metabolic interactions in a clostridial co-culture for consolidated bioprocessing.

    abstract：BACKGROUND:Clostridial co-culture containing cellulolytic and solventogenic species is a potential consolidated bioprocessing (CBP) approach for producing biochemicals and biofuels from cellulosic biomass. It has been demonstrated that the rate of cellulose utilization in the co-culture of Clostridium acetobutylicum an...

    journal_title：BMC biotechnology

    pub_type： 杂志文章

    doi：10.1186/1472-6750-13-95

    authors： Salimi F,Mahadevan R

    更新日期：2013-11-04 00:00:00

  • Effects of DNA mass on multiple displacement whole genome amplification and genotyping performance.

    abstract：BACKGROUND:Whole genome amplification (WGA) promises to eliminate practical molecular genetic analysis limitations associated with genomic DNA (gDNA) quantity. We evaluated the performance of multiple displacement amplification (MDA) WGA using gDNA extracted from lymphoblastoid cell lines (N = 27) with a range of start...

    journal_title：BMC biotechnology

    pub_type： 杂志文章

    doi：10.1186/1472-6750-5-24

    authors： Bergen AW,Qi Y,Haque KA,Welch RA,Chanock SJ

    更新日期：2005-09-16 00:00:00

  • Pattern analysis approach reveals restriction enzyme cutting abnormalities and other cDNA library construction artifacts using raw EST data.

    abstract：BACKGROUND:Expressed Sequence Tag (EST) sequences are widely used in applications such as genome annotation, gene discovery and gene expression studies. However, some of GenBank dbEST sequences have proven to be "unclean". Identification of cDNA termini/ends and their structures in raw ESTs not only facilitates data qu...

    journal_title：BMC biotechnology

    pub_type： 杂志文章

    doi：10.1186/1472-6750-12-16

    authors： Zhou S,Ji G,Liu X,Li P,Moler J,Karro JE,Liang C

    更新日期：2012-05-03 00:00:00

  • Controlling pH in shake flasks using polymer-based controlled-release discs with pre-determined release kinetics.

    abstract：BACKGROUND:There are significant differences in the culture conditions between small-scale screenings and large-scale fermentation processes. Production processes are usually conducted in fed-batch cultivation mode with active pH-monitoring and control. In contrast, screening experiments in shake flasks are usually con...

    journal_title：BMC biotechnology

    pub_type： 杂志文章

    doi：10.1186/1472-6750-11-25

    authors： Scheidle M,Dittrich B,Klinger J,Ikeda H,Klee D,Büchs J

    更新日期：2011-03-23 00:00:00

  • Glycosyltransferase efficiently controls phenylpropanoid pathway.

    abstract：BACKGROUND:In a previous study, anthocyanin levels in potato plants were increased by manipulating genes connected with the flavonoid biosynthesis pathway. However, starch content and tuber yield were dramatically reduced in the transgenic plants, which over-expressed dihydroflavonol reductase (DFR). RESULTS:Transgeni...

    journal_title：BMC biotechnology

    pub_type： 杂志文章

    doi：10.1186/1472-6750-8-25

    authors： Aksamit-Stachurska A,Korobczak-Sosna A,Kulma A,Szopa J

    更新日期：2008-03-05 00:00:00

  • The CD-loop of PAI-2 (SERPINB2) is redundant in the targeting, inhibition and clearance of cell surface uPA activity.

    abstract：BACKGROUND:Plasminogen activator inhibitor type-2 (PAI-2, SERPINB2) is an irreversible, specific inhibitor of the urokinase plasminogen activator (uPA). Since overexpression of uPA at the surface of cancer cells is linked to malignancy, targeting of uPA by exogenous recombinant PAI-2 has been proposed as the basis of p...

    journal_title：BMC biotechnology

    pub_type： 杂志文章

    doi：10.1186/1472-6750-9-43

    authors： Cochran BJ,Gunawardhana LP,Vine KL,Lee JA,Lobov S,Ranson M

    更新日期：2009-05-14 00:00:00

  • Reliable handling of highly A/T-rich genomic DNA for efficient generation of knockin strains of Dictyostelium discoideum.

    abstract：BACKGROUND:Social amoeba, Dictyostelium discoideum, is a well-established model organism for studying cellular physiology and developmental pattern formation. Its haploid genome facilitates functional analysis of genes by a single round of mutagenesis including targeted disruption. Although the efficient generation of ...

    journal_title：BMC biotechnology

    pub_type： 杂志文章

    doi：10.1186/s12896-016-0267-8

    authors： Mukai A,Ichiraku A,Horikawa K

    更新日期：2016-04-14 00:00:00

  • Determination of some significant batch culture conditions affecting acetyl-xylan esterase production by Penicillium notatum NRRL-1249.

    abstract：BACKGROUND:Acetyl-xylan esterase (AXE, EC 3.1.1.72) hydrolyses acetate group from the linear chain of xylopyranose residues bound by β-1,4-linkage. The enzyme finds commercial applications in bio-bleaching of wood pulp, treating animal feed to increase digestibility, processing food to increase clarification and conver...

    journal_title：BMC biotechnology

    pub_type： 杂志文章

    doi：10.1186/1472-6750-11-52

    authors： Atta S,Ali S,Akhtar MN,Haq I

    更新日期：2011-05-16 00:00:00

  • Characterization of norovirus RNA replicase for in vitro amplification of RNA.

    abstract：BACKGROUND:The isothermal amplification of RNA in vitro has been used for the study of in vitro evolution of RNA. Although Qβ replicase has been traditionally used as an enzyme for this purpose, we planned to use norovirus replicase (NV3D(pol)) due to its structural simplicity in the scope of in vitro autonomous evolut...

    journal_title：BMC biotechnology

    pub_type： 杂志文章

    doi：10.1186/1472-6750-13-85

    authors： Arai H,Nishigaki K,Nemoto N,Suzuki M,Husimi Y

    更新日期：2013-10-09 00:00:00

  • A novel serine hydroxymethyltransferase from Arthrobacter nicotianae: characterization and improving catalytic efficiency by rational design.

    abstract：BACKGROUND:Serine hydroxymethyltransferase (SHMT) is the key enzyme in L-serine enzymatic production, suggesting the importance of obtaining a SHMT with high activity. RESULTS:Here, a novel SHMT gene, glyA, was obtained through degenerate oligonucleotide-primed PCR and encoded a novel SHMT with 54.3% similarity to the...

    journal_title：BMC biotechnology

    pub_type： 杂志文章

    doi：10.1186/s12896-014-0093-9

    authors： Jiang W,Chen L,Hu N,Yuan S,Li B,Liu Z

    更新日期：2014-11-14 00:00:00

  • Genetic transformation of einkorn (Triticum monococcum L. ssp. monococcum L.), a diploid cultivated wheat species.

    abstract：BACKGROUND:Domesticated einkorn (Triticum monococcum L.) is one of the oldest cultivated cereal crops in the world. Its small genome size (~ 5.7 GB), low ploidy (2n = 2x = 14, AmAm) and high genetic polymorphism make this species very attractive for use as a diploid model for understanding the genomics and proteomics o...

    journal_title：BMC biotechnology

    pub_type： 杂志文章

    doi：10.1186/s12896-018-0477-3

    authors： Miroshnichenko D,Ashin D,Pushin A,Dolgov S

    更新日期：2018-10-23 00:00:00

  • Effect of Fe nanoparticle on growth and glycolipid biosurfactant production under solid state culture by marine Nocardiopsis sp. MSA13A.

    abstract：BACKGROUND:Iron is an essential element in several pathways of microbial metabolism, and therefore low iron toxicity is expected on the usage of Fe nanoparticles (NPs). This study aims to determine the effect of Fe NPs on biosurfactant production by marine actinobacterium Nocardiopsis sp. MSA13A under solid state cultu...

    journal_title：BMC biotechnology

    pub_type： 杂志文章

    doi：10.1186/1472-6750-14-48

    authors： Kiran GS,Nishanth LA,Priyadharshini S,Anitha K,Selvin J

    更新日期：2014-05-21 00:00:00

  • Perlecan domain 1 recombinant proteoglycan augments BMP-2 activity and osteogenesis.

    abstract：BACKGROUND:Many growth factors, such as bone morphogenetic protein (BMP)-2, have been shown to interact with polymers of sulfated disacharrides known as heparan sulfate (HS) glycosaminoglycans (GAGs), which are found on matrix and cell-surface proteoglycans throughout the body. HS GAGs, and some more highly sulfated fo...

    journal_title：BMC biotechnology

    pub_type： 杂志文章

    doi：10.1186/1472-6750-12-60

    authors： Decarlo AA,Belousova M,Ellis AL,Petersen D,Grenett H,Hardigan P,O'Grady R,Lord M,Whitelock JM

    更新日期：2012-09-11 00:00:00

  • Purification of the recombinant green fluorescent protein from tobacco plants using alcohol/salt aqueous two-phase system and hydrophobic interaction chromatography.

    abstract：BACKGROUND:The green fluorescent protein (GFP) has been regarded as a valuable tool and widely applied as a biomarker in medical applications and diagnostics. A cost-efficient upstream expression system and an inexpensive downstream purification process will meet the demands of the GFP protein with high-purity. RESULT...

    journal_title：BMC biotechnology

    pub_type： 杂志文章

    doi：10.1186/s12896-019-0590-y

    authors： Dong J,Ding X,Wang S

    更新日期：2019-12-09 00:00:00

  • Recombinant Expression and Bioactivity Comparison of Four Typical Fungal Immunomodulatory Proteins from Three Main Ganoderma Species.

    abstract：BACKGROUND:More than a dozen of fungal immunomodulatory proteins (FIPs) have been identified to date, most of which are from Ganoderma species. However, little is known about the similarities and differences between different Ganoderma FIPs' bioactivities. In the current study, two FIP genes termed FIP-gap1 and FIP-gap...

    journal_title：BMC biotechnology

    pub_type： 杂志文章

    doi：10.1186/s12896-018-0488-0

    authors： Qu ZW,Zhou SY,Guan SX,Gao R,Duan ZW,Zhang X,Sun WY,Fan WL,Chen SS,Chen LJ,Lin JW,Ruan YY

    更新日期：2018-12-14 00:00:00

  • Stable high-level expression of factor VIII in Chinese hamster ovary cells in improved elongation factor-1 alpha-based system.

    abstract：BACKGROUND:Recombinant factor VIII (FVIII), used for haemophilia A therapy, is one of the most challenging among the therapeutic proteins produced in heterologous expression systems. Deletion variant of FVIII, in which the entire domain B is replaced by a short linker peptide, was approved for medical use. Efficacy and...

    journal_title：BMC biotechnology

    pub_type： 杂志文章

    doi：10.1186/s12896-017-0353-6

    authors： Orlova NA,Kovnir SV,Gabibov AG,Vorobiev II

    更新日期：2017-03-24 00:00:00

  • Improved CRISPR/Cas9 gene editing by fluorescence activated cell sorting of green fluorescence protein tagged protoplasts.

    abstract：BACKGROUND:CRISPR/Cas9 is widely used for precise genetic editing in various organisms. CRISPR/Cas9 editing may in many plants be hampered by the presence of complex and high ploidy genomes and inefficient or poorly controlled delivery of the CRISPR/Cas9 components to gamete cells or cells with regenerative potential. ...

    journal_title：BMC biotechnology

    pub_type： 杂志文章

    doi：10.1186/s12896-019-0530-x

    authors： Petersen BL,Möller SR,Mravec J,Jørgensen B,Christensen M,Liu Y,Wandall HH,Bennett EP,Yang Z

    更新日期：2019-06-17 00:00:00

  • A novel adenoviral vector carrying an all-in-one Tet-On system with an autoregulatory loop for tight, inducible transgene expression.

    abstract：BACKGROUND:One of the most commonly used vectors for gene therapy is the adenoviral vector; its ability to tightly regulate transgene expression is critical for optimizing therapeutic outcomes. The tetracycline-regulated system (especially the Tet-On system) for gene expression is one of the most valuable tools for con...

    journal_title：BMC biotechnology

    pub_type： 杂志文章

    doi：10.1186/s12896-015-0121-4

    authors： Chen H,Wang D,Xia R,Mao Q,Xia H

    更新日期：2015-02-13 00:00:00

  • Heterogeneous oxygen availability affects the titer and topology but not the fidelity of plasmid DNA produced by Escherichia coli.

    abstract：BACKGROUND:Dissolved oxygen tension (DOT) is hardly constant and homogenously distributed in a bioreactor, which can have a negative impact in the metabolism and product synthesis. However, the effects of DOT on plasmid DNA (pDNA) production and quality have not been thoroughly investigated. In the present study, the e...

    journal_title：BMC biotechnology

    pub_type： 杂志文章

    doi：10.1186/s12896-017-0378-x

    authors： Jaén KE,Sigala JC,Olivares-Hernández R,Niehaus K,Lara AR

    更新日期：2017-07-04 00:00:00

  • Expression of Vitreoscilla hemoglobin enhances production of arachidonic acid and lipids in Mortierella alpina.

    abstract：BACKGROUND:Arachidonic acid (ARA, C20:4, n-6), which belongs to the omega-6 series of polyunsaturated fatty acids and has a variety of biological activities, is commercially produced in Mortierella alpina. Dissolved oxygen or oxygen utilization efficiency is a critical factor for Mortierella alpina growth and arachidon...

    journal_title：BMC biotechnology

    pub_type： 杂志文章

    doi：10.1186/s12896-017-0388-8

    authors： Zhang H,Feng Y,Cui Q,Song X

    更新日期：2017-08-30 00:00:00