Abstract:
BACKGROUND:Nanoparticles appear to be promising devices for application in the agriculture and food industries, but information regarding the response of plants to contact with nano-devices is scarce. Toxic effects may be imposed depending on the type and concentration of nanoparticle as well as time of exposure. A number of mechanisms may underlie the ability of nanoparticles to cause genotoxicity, besides the activation of ROS scavenging mechanisms. In a previous study, we showed that plant cells accumulate 3-Mercaptopropanoic acid-CdSe/ZnS quantum dots (MPA-CdSe/ZnS QD) in their cytosol and nucleus and increased production of ROS in a dose dependent manner when exposed to QD and that a concentration of 10 nM should be cyto-compatible. RESULTS:When Medicago sativa cells were exposed to 10, 50 and 100 nM MPA-CdSe/ZnS QD a correspondent increase in the activity of Superoxide dismutase, Catalase and Glutathione reductase was registered. Different versions of the COMET assay were used to assess the genotoxicity of MPA-CdSe/ZnS QD. The number of DNA single and double strand breaks increased with increasing concentrations of MPA-CdSe/ZnS QD. At the highest concentrations, tested purine bases were more oxidized than the pyrimidine ones. The transcription of the DNA repair enzymes Formamidopyrimidine DNA glycosylase, Tyrosyl-DNA phosphodiesterase I and DNA Topoisomerase I was up-regulated in the presence of increasing concentrations of MPA-CdSe/ZnS QD. CONCLUSIONS:Concentrations as low as 10 nM MPA-CdSe/ZnS Quantum Dots are cytotoxic and genotoxic to plant cells, although not lethal. This sets a limit for the concentrations to be used when practical applications using nanodevices of this type on plants are being considered. This work describes for the first time the genotoxic effect of Quantum Dots in plant cells and demonstrates that both the DNA repair genes (Tdp1β, Top1β and Fpg) and the ROS scavenging mechanisms are activated when MPA-CdSe/ZnS QD contact M. sativa cells.
journal_name
BMC Biotechnoljournal_title
BMC biotechnologyauthors
Santos AR,Miguel AS,Macovei A,Maycock C,Balestrazzi A,Oliva A,Fevereiro Pdoi
10.1186/1472-6750-13-111subject
Has Abstractpub_date
2013-12-20 00:00:00pages
111issn
1472-6750pii
1472-6750-13-111journal_volume
13pub_type
杂志文章abstract:BACKGROUND:Wax esters are important ingredients in cosmetics, pharmaceuticals, lubricants and other chemical industries due to their excellent wetting property. Since the naturally occurring wax esters are expensive and scarce, these esters can be produced by enzymatic alcoholysis of vegetable oils. In an enzymatic rea...
journal_title:BMC biotechnology
pub_type: 杂志文章
doi:10.1186/1472-6750-7-53
更新日期:2007-08-30 00:00:00
abstract:BACKGROUND:Xenoantigens are a major source of concern with regard to the success of interspecific xenografts. GGTA1 encodes α1,3-galactosyltransferase, which is essential for the biosynthesis of galactosyl-alpha 1,3-galactose, the major xenoantigen causing hyperacute rejection. GGTA1-modified pigs, therefore, are promi...
journal_title:BMC biotechnology
pub_type: 杂志文章
doi:10.1186/s12896-020-00638-7
更新日期:2020-08-18 00:00:00
abstract:BACKGROUND:Bacterial degradation/transformation of steroids is widely investigated to create biotechnologically relevant strains for industrial application. The strain of Nocardioides simplex VKM Ac-2033D is well known mainly for its superior 3-ketosteroid Δ1-dehydrogenase activity towards various 3-oxosteroids and oth...
journal_title:BMC biotechnology
pub_type: 杂志文章
doi:10.1186/s12896-021-00668-9
更新日期:2021-01-13 00:00:00
abstract:BACKGROUND:Polymerase chain reaction (PCR) is one of the most important developments in modern biotechnology. However, PCR is known to introduce biases, especially during multiplex reactions. Recent studies have implicated the DNA polymerase as the primary source of bias, particularly initiation of polymerization on th...
journal_title:BMC biotechnology
pub_type: 杂志文章
doi:10.1186/1472-6750-14-10
更新日期:2014-01-30 00:00:00
abstract:BACKGROUND:Fungal laccases are multicopper oxidases with huge applicability in different sectors. Here, we describe the development of a set of high-throughput colorimetric assays for screening laccase libraries in directed evolution studies. RESULTS:Firstly, we designed three colorimetric assays based on the oxidatio...
journal_title:BMC biotechnology
pub_type: 杂志文章
doi:10.1186/1472-6750-13-90
更新日期:2013-10-26 00:00:00
abstract:BACKGROUND:Aldehyde-deformylating oxygenase (ADO) is a key enzyme involved in the biosynthetic pathway of fatty alk(a/e)nes in cyanobacteria. However, cADO (cyanobacterial ADO) showed extreme low activity with the k cat value below 1 min-1, which would limit its application in biofuel production. To identify the activi...
journal_title:BMC biotechnology
pub_type: 杂志文章
doi:10.1186/s12896-017-0351-8
更新日期:2017-03-16 00:00:00
abstract:BACKGROUND:Urease B is an important virulence factor that is required for Helicobacter pylori to colonise the gastric mucosa. Mouse monoclonal antibodies (mAbs) that inhibit urease B enzymatic activity will be useful as vaccines for the prevention and treatment of H. pylori infection. Here, we produced murine mAbs agai...
journal_title:BMC biotechnology
pub_type: 杂志文章
doi:10.1186/1472-6750-10-84
更新日期:2010-11-30 00:00:00
abstract:BACKGROUND:Ligand-targeted approaches have proven successful in improving the therapeutic index of a number of drugs. We hypothesized that the specific targeting of TNF-alpha antagonists to inflamed tissues could increase drug efficacy and reduce side effects. RESULTS:Using uteroglobin (UG), a potent anti-inflammatory...
journal_title:BMC biotechnology
pub_type: 杂志文章
doi:10.1186/1472-6750-11-104
更新日期:2011-11-10 00:00:00
abstract:BACKGROUND:Prostate cancer is the most-diagnosed non-skin cancer among males in the US, and the second leading cause of cancer-related death. Current methods of treatment and diagnosis are not specific for the disease. This work identified an antibody fragment that binds selectively to a molecule on the surface of andr...
journal_title:BMC biotechnology
pub_type: 杂志文章
doi:10.1186/1472-6750-14-81
更新日期:2014-09-03 00:00:00
abstract:BACKGROUND:Despite positive reports on the efficacy of stem cell therapy for the treatment of cardiovascular disease, the nature of stem cell homing to ischemic tissues remains elusive. RESULTS:We used a mouse model of peripheral tissue ischemia to study the survival and homing capacity of dual reporter gene (eGFP/Luc...
journal_title:BMC biotechnology
pub_type: 杂志文章
doi:10.1186/1472-6750-12-93
更新日期:2012-12-03 00:00:00
abstract:BACKGROUND:The use of small interfering RNA (siRNA) molecules in animals to achieve double-stranded RNA-mediated interference (RNAi) has recently emerged as a powerful method of sequence-specific gene knockdown. As DNA-based expression of short hairpin RNA (shRNA) for RNAi may offer some advantages over chemical and in...
journal_title:BMC biotechnology
pub_type: 杂志文章
doi:10.1186/1472-6750-5-13
更新日期:2005-05-11 00:00:00
abstract:BACKGROUND:The safety of mutagenized and genetically transformed plants remains a subject of scrutiny. Data gathered and communicated on the phenotypic and molecular variation induced by gene transfer technologies will provide a scientific-based means to rationally address such concerns. In this study, genomic structur...
journal_title:BMC biotechnology
pub_type: 杂志文章
doi:10.1186/s12896-016-0271-z
更新日期:2016-05-12 00:00:00
abstract:BACKGROUND:Acetyl-xylan esterase (AXE, EC 3.1.1.72) hydrolyses acetate group from the linear chain of xylopyranose residues bound by β-1,4-linkage. The enzyme finds commercial applications in bio-bleaching of wood pulp, treating animal feed to increase digestibility, processing food to increase clarification and conver...
journal_title:BMC biotechnology
pub_type: 杂志文章
doi:10.1186/1472-6750-11-52
更新日期:2011-05-16 00:00:00
abstract:BACKGROUND:The CRISPR-Cas9 system is a powerful and versatile tool for crop genome editing. However, achieving highly efficient and specific editing in polyploid species can be a challenge. The efficiency and specificity of the CRISPR-Cas9 system depends critically on the gRNA used. Here, we assessed the activities and...
journal_title:BMC biotechnology
pub_type: 杂志文章
doi:10.1186/s12896-019-0565-z
更新日期:2019-10-30 00:00:00
abstract:BACKGROUND:Interest in using plants for production of recombinant proteins such as monoclonal antibodies is growing, but proteolytic degradation, leading to a loss of functionality and complications in downstream purification, is still a serious problem. RESULTS:In this study, we investigated the dynamics of the assem...
journal_title:BMC biotechnology
pub_type: 杂志文章
doi:10.1186/1472-6750-11-128
更新日期:2011-12-30 00:00:00
abstract:BACKGROUND:A modification of a standard method of fluorescence in situ hybridisation (FISH) is described, by which a combination of several substrates and probes on single microscope slides enables more accurate comparisons of the distribution and abundance of chromosomal sequences and improves the relatively low throu...
journal_title:BMC biotechnology
pub_type: 杂志文章
doi:10.1186/1472-6750-6-20
更新日期:2006-03-20 00:00:00
abstract:BACKGROUND:Mesenchymal stem cells (MSCs) are of great interest in bone regenerative medicine due to their osteogenic potential and trophic effects. However, challenges to large-scale production of MSCs can hinder the translation of MSC therapies. 3D Microcarrier (MC)-based MSC culture presents a scalable and cost-effec...
journal_title:BMC biotechnology
pub_type: 杂志文章
doi:10.1186/s12896-015-0219-8
更新日期:2015-10-31 00:00:00
abstract:BACKGROUND:As with many plant species, current genome editing strategies in soybean are initiated by stably transforming a gene that encodes an engineered nuclease into the genome. Expression of the transgene results in a double-stranded break and repair at the targeted locus, oftentimes resulting in mutation(s) at the...
journal_title:BMC biotechnology
pub_type: 杂志文章
doi:10.1186/s12896-020-00604-3
更新日期:2020-02-24 00:00:00
abstract:BACKGROUND:Mesenchymal stem cells (MSCs) are an attractive source of adult stem cells for therapeutic application in clinical study. Genetic modification of MSCs with beneficial genes makes them more effective for therapeutic use. However, it is difficult to transduce genes into MSCs by common transfection methods, esp...
journal_title:BMC biotechnology
pub_type: 杂志文章
doi:10.1186/1472-6750-10-38
更新日期:2010-05-13 00:00:00
abstract:BACKGROUND:The ubiquitin protein is present in all eukaryotic cells and promoters from ubiquitin genes are good candidates to regulate the constitutive expression of transgenes in plants. Therefore, two switchgrass (Panicum virgatum L.) ubiquitin genes (PvUbi1 and PvUbi2) were cloned and characterized. Reporter constru...
journal_title:BMC biotechnology
pub_type: 杂志文章
doi:10.1186/1472-6750-11-74
更新日期:2011-07-11 00:00:00
abstract:BACKGROUND:There is an increasing need to understand cell-cell interactions for cell and tissue engineering purposes, such as optimizing cell sheet constructs, as well as for examining adhesion defect diseases. For cell-sheet engineering, one major obstacle to sheet function is that cell sheets in suspension are fragil...
journal_title:BMC biotechnology
pub_type: 杂志文章
doi:10.1186/1472-6750-13-17
更新日期:2013-02-26 00:00:00
abstract:BACKGROUND:The green fluorescent protein (GFP) has been regarded as a valuable tool and widely applied as a biomarker in medical applications and diagnostics. A cost-efficient upstream expression system and an inexpensive downstream purification process will meet the demands of the GFP protein with high-purity. RESULT...
journal_title:BMC biotechnology
pub_type: 杂志文章
doi:10.1186/s12896-019-0590-y
更新日期:2019-12-09 00:00:00
abstract:BACKGROUND:The cattle ticks, Boophilus spp., affect cattle production in tropical and subtropical regions of the world. Tick vaccines constitute a cost-effective and environmentally friendly alternative to tick control. The recombinant B. microplus Bm86 protective antigen has been shown to protect cattle against tick i...
journal_title:BMC biotechnology
pub_type: 杂志文章
doi:10.1186/1472-6750-9-29
更新日期:2009-03-31 00:00:00
abstract:BACKGROUND:Dengue is a public health problem of global significance for which there is neither an effective antiviral therapy nor a preventive vaccine. It is a mosquito-borne viral disease, caused by dengue (DEN) viruses, which are members of the Flaviviridae family. There are four closely related serotypes, DEN-1, DEN...
journal_title:BMC biotechnology
pub_type: 杂志文章
doi:10.1186/1472-6750-7-10
更新日期:2007-02-15 00:00:00
abstract:BACKGROUND:β-Fructofuranosidases (or invertases) catalyse the commercially-important biotransformation of sucrose into short-chain fructooligosaccharides with wide-scale application as a prebiotic in the functional foods and pharmaceutical industries. RESULTS:We identified a β-fructofuranosidase gene (CmINV) from a Ce...
journal_title:BMC biotechnology
pub_type: 杂志文章
doi:10.1186/1472-6750-13-100
更新日期:2013-11-14 00:00:00
abstract:BACKGROUND:The recent H1N1 influenza pandemic illustrated the shortcomings of the vaccine manufacturing process. The A/California/07/2009 H1N1 pandemic influenza vaccine or A(H1N1)pdm09 was available late and in short supply as a result of delays in production caused by low yields and poor antigen stability. Recombinan...
journal_title:BMC biotechnology
pub_type: 杂志文章
doi:10.1186/1472-6750-12-77
更新日期:2012-10-30 00:00:00
abstract:BACKGROUND:Cell-penetrating peptides (CPPs) have been widely used as carriers to transport different molecules into living cells, whereas messenger RNAs (mRNAs) have been utilized as target molecules for the prevention and treatment of various diseases. However, the instability of CPPs and mRNAs has limited their appli...
journal_title:BMC biotechnology
pub_type: 杂志文章
doi:10.1186/s12896-016-0274-9
更新日期:2016-05-28 00:00:00
abstract:BACKGROUND:von Willebrand factor (VWF) is a key load bearing domain for mamalian cell adhesion by binding various macromolecular ligands in extracellular matrix such as, collagens, elastin, and glycosaminoglycans. Interestingly, vWF like domains are also commonly found in load bearing systems of marine organisms such a...
journal_title:BMC biotechnology
pub_type: 杂志文章
doi:10.1186/s12896-016-0247-z
更新日期:2016-02-16 00:00:00
abstract:BACKGROUND:In the last three decades, the Baculovirus expression vector system (BEV) has evolved to one of the most widely used eukaryotic systems for heterologous protein expression including approved vaccines and therapies. Despite the significant improvements introduced during the past years, the BEV system still ha...
journal_title:BMC biotechnology
pub_type: 杂志文章
doi:10.1186/s12896-017-0400-3
更新日期:2017-11-16 00:00:00
abstract:BACKGROUND:More than a dozen of fungal immunomodulatory proteins (FIPs) have been identified to date, most of which are from Ganoderma species. However, little is known about the similarities and differences between different Ganoderma FIPs' bioactivities. In the current study, two FIP genes termed FIP-gap1 and FIP-gap...
journal_title:BMC biotechnology
pub_type: 杂志文章
doi:10.1186/s12896-018-0488-0
更新日期:2018-12-14 00:00:00