Enhanced in vitro osteogenic differentiation of human fetal MSCs attached to 3D microcarriers versus harvested from 2D monolayers.


BACKGROUND:Mesenchymal stem cells (MSCs) are of great interest in bone regenerative medicine due to their osteogenic potential and trophic effects. However, challenges to large-scale production of MSCs can hinder the translation of MSC therapies. 3D Microcarrier (MC)-based MSC culture presents a scalable and cost-effective alternative to conventional methods of expansion in 2D monolayers. Furthermore, biodegradable MCs may allow for MC-bound MSC delivery without enzymatic harvest for selected applications such as bone healing. However, the effects of cell expansion on microcarriers and enzymatic cell harvest on MSC phenotype and osteogenic differential potential are not well understood. In this study, we characterized human fetal MSCs (hfMSCs) after expansion in 3D microcarrier spinner or 2D monolayer cultures. Following expansion, we compared osteogenic differentiation of cultures seeded with 3D MC-harvested, 3D MC-bound and conventional 2D monolayer (MNL)-harvested cells when cultured in osteogenic induction media on collagen-coated plates. RESULTS:Fetal MSCs expanded on both 3D agitated Microcarriers (MC) and 2D Plastic static monolayer (MNL) cultures express high levels of MSC surface markers. MC-harvested hfMSCs displayed higher expression of early osteogenic genes but slower mineralization kinetics compared to MNL-harvested MSCs during osteogenic induction. However, in the comparison between MC-bound and MC-harvested hfMSCs, osteogenic genes were upregulated and mineralization kinetics was accelerated in the former condition. Importantly, 3D MC-bound hfMSCs expressed higher levels of osteogenic genes and displayed either higher or equivalent levels of mineralization, depending on the cell line, compared to the classical monolayer cultures use in the literature (MNL-harvested hfMSCs). CONCLUSION:Beyond the processing and scalability advantages of the microcarrier culture, hfMSCs attached to MCs undergo robust osteogenic differentiation and mineralization compared to enzymatically harvested cells. Thus biodegradable/biocompatible MCs which can potentially be used for cell expansion as well as a scaffold for direct in vivo delivery of cells may have advantages over the current methods of monolayer-expansion and delivery post-harvest for bone regeneration applications.


BMC Biotechnol


BMC biotechnology


Shekaran A,Sim E,Tan KY,Chan JK,Choolani M,Reuveny S,Oh S




Has Abstract


2015-10-31 00:00:00










  • Evaluation of biolistic gene transfer methods in vivo using non-invasive bioluminescent imaging techniques.

    abstract:BACKGROUND:Gene therapy continues to hold great potential for treating many different types of disease and dysfunction. Safe and efficient techniques for gene transfer and expression in vivo are needed to enable gene therapeutic strategies to be effective in patients. Currently, the most commonly used methods employ re...

    journal_title:BMC biotechnology

    pub_type: 杂志文章


    authors: Xia J,Martinez A,Daniell H,Ebert SN

    更新日期:2011-06-02 00:00:00

  • Using quantitative real-time PCR to detect chimeras in transgenic tobacco and apricot and to monitor their dissociation.

    abstract:BACKGROUND:The routine generation of transgenic plants involves analysis of transgene integration into the host genome by means of Southern blotting. However, this technique cannot distinguish between uniformly transformed tissues and the presence of a mixture of transgenic and non-transgenic cells in the same tissue. ...

    journal_title:BMC biotechnology

    pub_type: 杂志文章


    authors: Faize M,Faize L,Burgos L

    更新日期:2010-07-16 00:00:00

  • Degradation or excretion of quantum dots in mouse embryonic stem cells.

    abstract:BACKGROUND:Quantum dots (QDs) have been considered as a new and efficient probe for labeling cells non-invasively in vitro and in vivo, but fairly little is known about how QDs are eliminated from cells after labeling. The purpose of this study is to investigate the metabolism of QDs in different type of cells. RESULT...

    journal_title:BMC biotechnology

    pub_type: 杂志文章


    authors: Pi QM,Zhang WJ,Zhou GD,Liu W,Cao Y

    更新日期:2010-05-06 00:00:00

  • Evaluating the use of a CpG free promoter for long-term recombinant protein expression stability in Chinese hamster ovary cells.

    abstract:BACKGROUND:Methylated CpG dinucleotides in promoters are associated with the loss of gene expression in recombinant Chinese hamster ovary (CHO) cells during large-scale commercial manufacturing. We evaluated a promoter devoid of CpG dinucleotides, CpGfree, in parallel with a similar CpG containing promoter, CpGrich, fo...

    journal_title:BMC biotechnology

    pub_type: 杂志文章


    authors: Ho SC,Koh EY,Soo BP,Mariati,Chao SH,Yang Y

    更新日期:2016-10-18 00:00:00

  • Comparison and transfer testing of multiplex ligation detection methods for GM plants.

    abstract:BACKGROUND:With the increasing number of GMOs on the global market the maintenance of European GMO regulations is becoming more complex. For the analysis of a single food or feed sample it is necessary to assess the sample for the presence of many GMO-targets simultaneously at a sensitive level. Several methods have be...

    journal_title:BMC biotechnology

    pub_type: 杂志文章


    authors: Ujhelyi G,Dijk JP,Prins TW,Voorhuijzen MM,Hoef AM,Beenen HG,Morisset D,Gruden K,Kok EJ

    更新日期:2012-01-19 00:00:00

  • Novel anti-CD38 humanized mAb SG003 possessed enhanced cytotoxicity in lymphoma than Daratumumab via antibody-dependent cell-mediated cytotoxicity.

    abstract:BACKGROUND:In vivo use of monoclonal antibodies has become routine clinical practice in the treatment of human cancer. CD38 is an attractive target, because it has double roles, as a receptor and an ectoenzyme. Daratumumab, an anti-CD38 antibody, is currently in the clinical trials for multiple myeloma. RESULTS:Here w...

    journal_title:BMC biotechnology

    pub_type: 杂志文章


    authors: Yu T,Qiao C,Lv M,Tang L

    更新日期:2019-05-22 00:00:00

  • Glycosyltransferase efficiently controls phenylpropanoid pathway.

    abstract:BACKGROUND:In a previous study, anthocyanin levels in potato plants were increased by manipulating genes connected with the flavonoid biosynthesis pathway. However, starch content and tuber yield were dramatically reduced in the transgenic plants, which over-expressed dihydroflavonol reductase (DFR). RESULTS:Transgeni...

    journal_title:BMC biotechnology

    pub_type: 杂志文章


    authors: Aksamit-Stachurska A,Korobczak-Sosna A,Kulma A,Szopa J

    更新日期:2008-03-05 00:00:00

  • Effect of the cancer specific shorter form of human 6-phosphofructo-1-kinase on the metabolism of the yeast Saccharomyces cerevisiae.

    abstract:BACKGROUND:At first glance, there appears to be a high degree of similarity between the metabolism of yeast (the Crabtree effect) and human cancer cells (the Warburg effect). At the root of both effects is accelerated metabolic flow through glycolysis which leads to overflows of ethanol and lactic acid, respectively. I...

    journal_title:BMC biotechnology

    pub_type: 杂志文章


    authors: Andrejc D,Možir A,Legiša M

    更新日期:2017-05-08 00:00:00

  • Pandemic influenza vaccine: characterization of A/California/07/2009 (H1N1) recombinant hemagglutinin protein and insights into H1N1 antigen stability.

    abstract:BACKGROUND:The recent H1N1 influenza pandemic illustrated the shortcomings of the vaccine manufacturing process. The A/California/07/2009 H1N1 pandemic influenza vaccine or A(H1N1)pdm09 was available late and in short supply as a result of delays in production caused by low yields and poor antigen stability. Recombinan...

    journal_title:BMC biotechnology

    pub_type: 杂志文章


    authors: Feshchenko E,Rhodes DG,Felberbaum R,McPherson C,Rininger JA,Post P,Cox MM

    更新日期:2012-10-30 00:00:00

  • Integration, abundance, and transmission of mutations and transgenes in a series of CRISPR/Cas9 soybean lines.

    abstract:BACKGROUND:As with many plant species, current genome editing strategies in soybean are initiated by stably transforming a gene that encodes an engineered nuclease into the genome. Expression of the transgene results in a double-stranded break and repair at the targeted locus, oftentimes resulting in mutation(s) at the...

    journal_title:BMC biotechnology

    pub_type: 杂志文章


    authors: Michno JM,Virdi K,Stec AO,Liu J,Wang X,Xiong Y,Stupar RM

    更新日期:2020-02-24 00:00:00

  • Genomic insertion of lentiviral DNA circles directed by the yeast Flp recombinase.

    abstract:BACKGROUND:Circular forms of viral genomic DNA are generated during infection of cells with retroviruses like HIV-1. Such circles are unable to replicate and are eventually lost as a result of cell division, lending support to the prevalent notion that episomal retroviral DNA forms are dead-end products of reverse tran...

    journal_title:BMC biotechnology

    pub_type: 杂志文章


    authors: Moldt B,Staunstrup NH,Jakobsen M,Yáñez-Muñoz RJ,Mikkelsen JG

    更新日期:2008-08-09 00:00:00

  • Generation of an external guide sequence library for a reverse genetic screen in Caenorhabditis elegans.

    abstract:BACKGROUND:A method for inhibiting the expression of particular genes using external guide sequences (EGSs) has been developed in bacteria, mammalian cells and maize cells. RESULTS:To examine whether EGS technology can be used to down-regulate gene expression in Caenorhabditis elegans (C. elegans), we generated EGS-Ng...

    journal_title:BMC biotechnology

    pub_type: 杂志文章


    authors: Yan Q,Zhao R,Zheng W,Yin C,Zhang B,Ma W

    更新日期:2009-05-20 00:00:00

  • Identification of residues important for the activity of aldehyde-deformylating oxygenase through investigation into the structure-activity relationship.

    abstract:BACKGROUND:Aldehyde-deformylating oxygenase (ADO) is a key enzyme involved in the biosynthetic pathway of fatty alk(a/e)nes in cyanobacteria. However, cADO (cyanobacterial ADO) showed extreme low activity with the k cat value below 1 min-1, which would limit its application in biofuel production. To identify the activi...

    journal_title:BMC biotechnology

    pub_type: 杂志文章


    authors: Wang Q,Bao L,Jia C,Li M,Li JJ,Lu X

    更新日期:2017-03-16 00:00:00

  • Effect of supercritical carbon dioxide on the enzymatic production of biodiesel from waste animal fat using immobilized Candida antarctica lipase B variant.

    abstract:BACKGROUND:Waste animal fat is a promising feedstock to replace vegetable oil that widely used in commercial biodiesel process, however the high content of free fatty acid in waste fat makes it unfeasible to be processed with commercial base-catalytic process. Enzymatic process is preferable to convert waste fat into b...

    journal_title:BMC biotechnology

    pub_type: 杂志文章


    authors: Pollardo AA,Lee HS,Lee D,Kim S,Kim J

    更新日期:2017-09-09 00:00:00

  • Association of high pressure and alkaline condition for solubilization of inclusion bodies and refolding of the NS1 protein from zika virus.

    abstract:BACKGROUND:Proteins in inclusion bodies (IBs) present native-like secondary structures. However, chaotropic agents at denaturing concentrations, which are widely used for IB solubilization and subsequent refolding, unfold these secondary structures. Removal of the chaotropes frequently causes reaggregation and poor rec...

    journal_title:BMC biotechnology

    pub_type: 杂志文章


    authors: Rosa da Silva CM,Chura-Chambi RM,Ramos Pereira L,Cordeiro Y,de Souza Ferreira LC,Morganti L

    更新日期:2018-12-12 00:00:00

  • Recombinant nucleases CEL I from celery and SP I from spinach for mutation detection.

    abstract:BACKGROUND:The detection of unknown mutations is important in research and medicine. For this purpose, a mismatch-specific endonuclease CEL I from celery has been established as a useful tool in high throughput projects. Previously, CEL I-like activities were described only in a variety of plants and could not be expre...

    journal_title:BMC biotechnology

    pub_type: 杂志文章


    authors: Pimkin M,Caretti E,Canutescu A,Yeung JB,Cohn H,Chen Y,Oleykowski C,Bellacosa A,Yeung AT

    更新日期:2007-06-01 00:00:00

  • Mimotopes selected with a neutralizing antibody against urease B from Helicobacter pylori induce enzyme inhibitory antibodies in mice upon vaccination.

    abstract:BACKGROUND:Urease B is an important virulence factor that is required for Helicobacter pylori to colonise the gastric mucosa. Mouse monoclonal antibodies (mAbs) that inhibit urease B enzymatic activity will be useful as vaccines for the prevention and treatment of H. pylori infection. Here, we produced murine mAbs agai...

    journal_title:BMC biotechnology

    pub_type: 杂志文章


    authors: Li Y,Ning Y,Wang Y,Peng D,Jiang Y,Zhang L,Long M,Luo J,Li M

    更新日期:2010-11-30 00:00:00

  • Comparative gene expression profiling of mouse ovaries upon stimulation with natural equine chorionic gonadotropin (N-eCG) and tethered recombinant-eCG (R-eCG).

    abstract:BACKGROUND:Equine chorionic gonadotropin (eCG) induces super-ovulation in laboratory animals. Notwithstanding its extensive usage, limited information is available regarding the differences between the in vivo effects of natural eCG (N-eCG) and recombinant eCG (R-eCG). This study aimed to investigate the gene expressio...

    journal_title:BMC biotechnology

    pub_type: 杂志文章


    authors: Min KS,Park JJ,Lee SY,Byambaragchaa M,Kang MH

    更新日期:2020-11-11 00:00:00

  • A novel expression system of domain I of human beta2 glycoprotein I in Escherichia coli.

    abstract:BACKGROUND:The antiphospholipid syndrome (APS), characterised by recurrent miscarriage and thrombosis, is a significant cause of morbidity and mortality. Domain I (DI) of human beta 2 glycoprotein I (beta2GPI) is thought to contain crucial antibody binding epitopes for antiphospholipid antibodies (aPL), which are criti...

    journal_title:BMC biotechnology

    pub_type: 杂志文章


    authors: Ioannou Y,Giles I,Lambrianides A,Richardson C,Pearl LH,Latchman DS,Isenberg DA,Rahman A

    更新日期:2006-02-10 00:00:00

  • Tetanus toxin fragments and Bcl-2 fusion proteins: cytoprotection and retrograde axonal migration.

    abstract:BACKGROUND:Tetanus neurotoxin (TeNT) is taken up at nerve terminals and undergoes retrograde migration. The toxic properties of TeNT reside in the toxin light chain (L), but like complete TeNT, the TeNT heavy chain (TTH) and the C-terminal domain (TTC) alone can bind and enter into neurons. Here, we explored whether at...

    journal_title:BMC biotechnology

    pub_type: 杂志文章


    authors: Watanabe Y,Matsuba T,Nakanishi M,Une M,Hanajima R,Nakashima K

    更新日期:2018-06-11 00:00:00

  • Generation and characterization of a human single-chain fragment variable (scFv) antibody against cytosine deaminase from yeast.

    abstract:BACKGROUND:The ability of cytosine deaminase (CD) to convert the antifungal agent 5-fluorocytosine (5-FC) into one of the most potent and largely used anticancer compound such as 5-fluorouracil (5-FU) raised considerable interest in this enzyme to model gene or antibody - directed enzyme-prodrug therapy (GDEPT/ADEPT) a...

    journal_title:BMC biotechnology

    pub_type: 杂志文章


    authors: Mallano A,Zamboni S,Carpinelli G,Santoro F,Flego M,Ascione A,Gellini M,Tombesi M,Podo F,Cianfriglia M

    更新日期:2008-09-10 00:00:00

  • The relationship between mTOR signalling pathway and recombinant antibody productivity in CHO cell lines.

    abstract:BACKGROUND:High recombinant protein productivity in mammalian cell lines is often associated with phenotypic changes in protein content, energy metabolism, and cell growth, but the key determinants that regulate productivity are still not clearly understood. The mammalian target of rapamycin (mTOR) signalling pathway h...

    journal_title:BMC biotechnology

    pub_type: 杂志文章


    authors: Edros R,McDonnell S,Al-Rubeai M

    更新日期:2014-02-17 00:00:00

  • CRISPR/Cas9-mediated genome editing induces gene knockdown by altering the pre-mRNA splicing in mice.

    abstract:BACKGROUND:Clustered Regularly Interspaced Short Palindromic Repeats/CRISPR associated protein 9 (CRISPR/Cas9) has been wildly used to generate gene knockout models through inducing indels causing frame-shift. However, there are few studies concerning the post-transcript effects caused by CRISPR-mediated genome editing...

    journal_title:BMC biotechnology

    pub_type: 杂志文章


    authors: Tang JX,Chen D,Deng SL,Li J,Li Y,Fu Z,Wang XX,Zhang Y,Chen SR,Liu YX

    更新日期:2018-10-03 00:00:00

  • Different genome-wide transcriptome responses of Nocardioides simplex VKM Ac-2033D to phytosterol and cortisone 21-acetate.

    abstract:BACKGROUND:Bacterial degradation/transformation of steroids is widely investigated to create biotechnologically relevant strains for industrial application. The strain of Nocardioides simplex VKM Ac-2033D is well known mainly for its superior 3-ketosteroid Δ1-dehydrogenase activity towards various 3-oxosteroids and oth...

    journal_title:BMC biotechnology

    pub_type: 杂志文章


    authors: Shtratnikova VY,Sсhelkunov MI,Fokina VV,Bragin EY,Shutov AA,Donova MV

    更新日期:2021-01-13 00:00:00

  • Reliable handling of highly A/T-rich genomic DNA for efficient generation of knockin strains of Dictyostelium discoideum.

    abstract:BACKGROUND:Social amoeba, Dictyostelium discoideum, is a well-established model organism for studying cellular physiology and developmental pattern formation. Its haploid genome facilitates functional analysis of genes by a single round of mutagenesis including targeted disruption. Although the efficient generation of ...

    journal_title:BMC biotechnology

    pub_type: 杂志文章


    authors: Mukai A,Ichiraku A,Horikawa K

    更新日期:2016-04-14 00:00:00

  • Ultrasound and microbubble induced release from intracellular compartments.

    abstract:BACKGROUND:Ultrasound and microbubbles (USMB) have been shown to enhance the intracellular uptake of molecules, generally thought to occur as a result of sonoporation. The underlying mechanism associated with USMB-enhanced intracellular uptake such as membrane disruption and endocytosis may also be associated with USMB...

    journal_title:BMC biotechnology

    pub_type: 杂志文章


    authors: Hussein F,Antonescu C,Karshafian R

    更新日期:2017-05-18 00:00:00

  • Heterogeneous oxygen availability affects the titer and topology but not the fidelity of plasmid DNA produced by Escherichia coli.

    abstract:BACKGROUND:Dissolved oxygen tension (DOT) is hardly constant and homogenously distributed in a bioreactor, which can have a negative impact in the metabolism and product synthesis. However, the effects of DOT on plasmid DNA (pDNA) production and quality have not been thoroughly investigated. In the present study, the e...

    journal_title:BMC biotechnology

    pub_type: 杂志文章


    authors: Jaén KE,Sigala JC,Olivares-Hernández R,Niehaus K,Lara AR

    更新日期:2017-07-04 00:00:00

  • Decreasing translation error rate in Escherichia coli increases protein function.

    abstract:BACKGROUND:Over-expressed native or recombinant proteins are commonly used for industrial and pharmaceutical purposes, as well as for research. Proteins of interest need to be purified in sufficient quantity, quality and specific activity to justify their commercial price and eventual medical use. Proteome quality was ...

    journal_title:BMC biotechnology

    pub_type: 杂志文章


    authors: Musa M,Radman M,Krisko A

    更新日期:2016-03-11 00:00:00

  • Oriented antibody immobilization to polystyrene macrocarriers for immunoassay modified with hydrazide derivatives of poly(meth)acrylic acid.

    abstract:BACKGROUND:Hydrophobic polystyrene is the most common material for solid phase immunoassay. Proteins are immobilized on polystyrene by passive adsorption, which often causes considerable denaturation. Biological macromolecules were found to better retain their functional activity when immobilized on hydrophilic materia...

    journal_title:BMC biotechnology

    pub_type: 杂志文章


    authors: Shmanai VV,Nikolayeva TA,Vinokurova LG,Litoshka AA

    更新日期:2001-01-01 00:00:00

  • Comparison of estimation capabilities of response surface methodology (RSM) with artificial neural network (ANN) in lipase-catalyzed synthesis of palm-based wax ester.

    abstract:BACKGROUND:Wax esters are important ingredients in cosmetics, pharmaceuticals, lubricants and other chemical industries due to their excellent wetting property. Since the naturally occurring wax esters are expensive and scarce, these esters can be produced by enzymatic alcoholysis of vegetable oils. In an enzymatic rea...

    journal_title:BMC biotechnology

    pub_type: 杂志文章


    authors: Basri M,Rahman RN,Ebrahimpour A,Salleh AB,Gunawan ER,Rahman MB

    更新日期:2007-08-30 00:00:00