Enhanced in vitro osteogenic differentiation of human fetal MSCs attached to 3D microcarriers versus harvested from 2D monolayers.


BACKGROUND:Mesenchymal stem cells (MSCs) are of great interest in bone regenerative medicine due to their osteogenic potential and trophic effects. However, challenges to large-scale production of MSCs can hinder the translation of MSC therapies. 3D Microcarrier (MC)-based MSC culture presents a scalable and cost-effective alternative to conventional methods of expansion in 2D monolayers. Furthermore, biodegradable MCs may allow for MC-bound MSC delivery without enzymatic harvest for selected applications such as bone healing. However, the effects of cell expansion on microcarriers and enzymatic cell harvest on MSC phenotype and osteogenic differential potential are not well understood. In this study, we characterized human fetal MSCs (hfMSCs) after expansion in 3D microcarrier spinner or 2D monolayer cultures. Following expansion, we compared osteogenic differentiation of cultures seeded with 3D MC-harvested, 3D MC-bound and conventional 2D monolayer (MNL)-harvested cells when cultured in osteogenic induction media on collagen-coated plates. RESULTS:Fetal MSCs expanded on both 3D agitated Microcarriers (MC) and 2D Plastic static monolayer (MNL) cultures express high levels of MSC surface markers. MC-harvested hfMSCs displayed higher expression of early osteogenic genes but slower mineralization kinetics compared to MNL-harvested MSCs during osteogenic induction. However, in the comparison between MC-bound and MC-harvested hfMSCs, osteogenic genes were upregulated and mineralization kinetics was accelerated in the former condition. Importantly, 3D MC-bound hfMSCs expressed higher levels of osteogenic genes and displayed either higher or equivalent levels of mineralization, depending on the cell line, compared to the classical monolayer cultures use in the literature (MNL-harvested hfMSCs). CONCLUSION:Beyond the processing and scalability advantages of the microcarrier culture, hfMSCs attached to MCs undergo robust osteogenic differentiation and mineralization compared to enzymatically harvested cells. Thus biodegradable/biocompatible MCs which can potentially be used for cell expansion as well as a scaffold for direct in vivo delivery of cells may have advantages over the current methods of monolayer-expansion and delivery post-harvest for bone regeneration applications.


BMC Biotechnol


BMC biotechnology


Shekaran A,Sim E,Tan KY,Chan JK,Choolani M,Reuveny S,Oh S




Has Abstract


2015-10-31 00:00:00










  • An engineered pathway for glyoxylate metabolism in tobacco plants aimed to avoid the release of ammonia in photorespiration.

    abstract:BACKGROUND:The photorespiratory nitrogen cycle in C₃ plants involves an extensive diversion of carbon and nitrogen away from the direct pathways of assimilation. The liberated ammonia is re-assimilated, but up to 25% of the carbon may be released into the atmosphere as CO₂. Because of the loss of CO₂ and high energy co...

    journal_title:BMC biotechnology

    pub_type: 杂志文章


    authors: Carvalho Jde F,Madgwick PJ,Powers SJ,Keys AJ,Lea PJ,Parry MA

    更新日期:2011-11-21 00:00:00

  • Expression of HPV-11 L1 protein in transgenic Arabidopsis thaliana and Nicotiana tabacum.

    abstract:BACKGROUND:We have investigated the possibility and feasibility of producing the HPV-11 L1 major capsid protein in transgenic Arabidopsis thaliana ecotype Columbia and Nicotiana tabacum cv. Xanthi as potential sources for an inexpensive subunit vaccine. RESULTS:Transformation of plants was only achieved with the HPV-1...

    journal_title:BMC biotechnology

    pub_type: 杂志文章


    authors: Kohl TO,Hitzeroth II,Christensen ND,Rybicki EP

    更新日期:2007-09-12 00:00:00

  • Construction and characterization of a high-quality cDNA library of Cymbidium faberi suitable for yeast one- and two-hybrid assays.

    abstract:BACKGROUND:Cymbidium faberi is one of the oldest cultivars of oriental orchids, with an elegant flower fragrance. In order to investigate the molecular mechanism and the functions of related proteins in the methyl jasmonate (MeJA) signaling pathway, one of the main components of flower fragrance in C. faberi, yeast one...

    journal_title:BMC biotechnology

    pub_type: 杂志文章


    authors: Xu Y,Zhou J,Liu Q,Li K,Zhou Y

    更新日期:2020-01-16 00:00:00

  • Manipulation of gene expression by an ecdysone-inducible gene switch in tumor xenografts.

    abstract:BACKGROUND:Rapid, robust and reversible induction of transgene expression would significantly facilitate cancer gene therapy as well as allow the in vivo functional study of newly discovered genes in tumor formation and progression. The popularity of the ecdysone inducible gene switch system has led us to investigate w...

    journal_title:BMC biotechnology

    pub_type: 杂志文章


    authors: Karns LR,Kisielewski A,Gulding KM,Seraj JM,Theodorescu D

    更新日期:2001-01-01 00:00:00

  • Secretion of Fc-amidated peptide fusion proteins by Chinese hamster ovary cells.

    abstract:BACKGROUND:The therapeutic use of α-amidated peptides (e.g. calcitonin, glucagon-like peptide) has increased dramatically, but there are major impediments to wider use of such peptides. Larger peptides are expensive to synthesize, and short plasma half-lives frequently limit the clinical circumstances in which the pept...

    journal_title:BMC biotechnology

    pub_type: 杂志文章


    authors: Carlson KR,Pomerantz SC,Li J,Vafa O,Naso M,Strohl W,Mains RE,Eipper BA

    更新日期:2015-06-27 00:00:00

  • Nicotiana benthamiana is a suitable transient system for high-level expression of an active inhibitor of cotton boll weevil α-amylase.

    abstract:BACKGROUND:Insect resistance in crops represents a main challenge for agriculture. Transgenic approaches based on proteins displaying insect resistance properties are widely used as efficient breeding strategies. To extend the spectrum of targeted pathogens and overtake the development of resistance, molecular evolutio...

    journal_title:BMC biotechnology

    pub_type: 杂志文章


    authors: Prado GS,Bamogo PKA,de Abreu JAC,Gillet FX,Dos Santos VO,Silva MCM,Brizard JP,Bemquerer MP,Bangratz M,Brugidou C,Sérémé D,Grossi-de-Sa MF,Lacombe S

    更新日期:2019-03-08 00:00:00

  • Membrane-bound and soluble forms of an NMDA receptor extracellular domain retain epitopes targeted in auto-immune encephalitis.

    abstract:BACKGROUND:Anti-NMDA receptor encephalitis (ANRE) is a potentially lethal disease attributed to auto-antibodies against the N-methyl-D-aspartate receptor (NMDAR). Full recovery is possible if therapy is initiated early in the disease course. Detection of ANRE antibodies in the cerebrospinal fluid (CSF) is essential for...

    journal_title:BMC biotechnology

    pub_type: 杂志文章


    authors: Sharma R,Al-Saleem FH,Puligedda RD,Rattelle A,Lynch DR,Dessain SK

    更新日期:2018-06-27 00:00:00

  • A novel adenoviral vector carrying an all-in-one Tet-On system with an autoregulatory loop for tight, inducible transgene expression.

    abstract:BACKGROUND:One of the most commonly used vectors for gene therapy is the adenoviral vector; its ability to tightly regulate transgene expression is critical for optimizing therapeutic outcomes. The tetracycline-regulated system (especially the Tet-On system) for gene expression is one of the most valuable tools for con...

    journal_title:BMC biotechnology

    pub_type: 杂志文章


    authors: Chen H,Wang D,Xia R,Mao Q,Xia H

    更新日期:2015-02-13 00:00:00

  • A Sleeping Beauty DNA transposon-based genetic sensor for functional screening of vitamin D3 analogues.

    abstract:BACKGROUND:Analogues of vitamin D3 are extensively used in the treatment of various illnesses, such as osteoporosis, inflammatory skin diseases, and cancer. Functional testing of new vitamin D3 analogues and formulations for improved systemic and topical administration is supported by sensitive screening methods that a...

    journal_title:BMC biotechnology

    pub_type: 杂志文章


    authors: Staunstrup NH,Sharma N,Bak RO,Svensson L,Petersen TK,Aarenstrup L,Kristiansen K,Bolund L,Mikkelsen JG

    更新日期:2011-04-07 00:00:00

  • Expression, intracellular targeting and purification of HIV Nef variants in tobacco cells.

    abstract:BACKGROUND:Plants may represent excellent alternatives to classical heterologous protein expression systems, especially for the production of biopharmaceuticals and vaccine components. Modern vaccines are becoming increasingly complex, with the incorporation of multiple antigens. Approaches towards developing an HIV va...

    journal_title:BMC biotechnology

    pub_type: 杂志文章


    authors: Marusic C,Nuttall J,Buriani G,Lico C,Lombardi R,Baschieri S,Benvenuto E,Frigerio L

    更新日期:2007-02-26 00:00:00

  • Identification of residues important for the activity of aldehyde-deformylating oxygenase through investigation into the structure-activity relationship.

    abstract:BACKGROUND:Aldehyde-deformylating oxygenase (ADO) is a key enzyme involved in the biosynthetic pathway of fatty alk(a/e)nes in cyanobacteria. However, cADO (cyanobacterial ADO) showed extreme low activity with the k cat value below 1 min-1, which would limit its application in biofuel production. To identify the activi...

    journal_title:BMC biotechnology

    pub_type: 杂志文章


    authors: Wang Q,Bao L,Jia C,Li M,Li JJ,Lu X

    更新日期:2017-03-16 00:00:00

  • Synergistic interaction of hTGF-β3 with hBMP-6 promotes articular cartilage formation in chitosan scaffolds with hADSCs: implications for regenerative medicine.

    abstract:BACKGROUND:Human TGF-β3 has been used in many studies to induce genes coding for typical cartilage matrix components and accelerate chondrogenic differentiation, making it the standard constituent in most cultivation media used for the assessment of chondrogenesis associated with various stem cell types on carrier matr...

    journal_title:BMC biotechnology

    pub_type: 杂志文章


    authors: Huang Y,Seitz D,Chevalier Y,Müller PE,Jansson V,Klar RM

    更新日期:2020-08-27 00:00:00

  • Single fluorescent protein-based Ca2+ sensors with increased dynamic range.

    abstract:BACKGROUND:Genetically encoded sensors developed on the basis of green fluorescent protein (GFP)-like proteins are becoming more and more popular instruments for monitoring cellular analytes and enzyme activities in living cells and transgenic organisms. In particular, a number of Ca2+ sensors have been developed, eith...

    journal_title:BMC biotechnology

    pub_type: 杂志文章


    authors: Souslova EA,Belousov VV,Lock JG,Strömblad S,Kasparov S,Bolshakov AP,Pinelis VG,Labas YA,Lukyanov S,Mayr LM,Chudakov DM

    更新日期:2007-06-29 00:00:00

  • Pandemic influenza vaccine: characterization of A/California/07/2009 (H1N1) recombinant hemagglutinin protein and insights into H1N1 antigen stability.

    abstract:BACKGROUND:The recent H1N1 influenza pandemic illustrated the shortcomings of the vaccine manufacturing process. The A/California/07/2009 H1N1 pandemic influenza vaccine or A(H1N1)pdm09 was available late and in short supply as a result of delays in production caused by low yields and poor antigen stability. Recombinan...

    journal_title:BMC biotechnology

    pub_type: 杂志文章


    authors: Feshchenko E,Rhodes DG,Felberbaum R,McPherson C,Rininger JA,Post P,Cox MM

    更新日期:2012-10-30 00:00:00

  • Characterization of norovirus RNA replicase for in vitro amplification of RNA.

    abstract:BACKGROUND:The isothermal amplification of RNA in vitro has been used for the study of in vitro evolution of RNA. Although Qβ replicase has been traditionally used as an enzyme for this purpose, we planned to use norovirus replicase (NV3D(pol)) due to its structural simplicity in the scope of in vitro autonomous evolut...

    journal_title:BMC biotechnology

    pub_type: 杂志文章


    authors: Arai H,Nishigaki K,Nemoto N,Suzuki M,Husimi Y

    更新日期:2013-10-09 00:00:00

  • Enhancing the thermostability of α-L-rhamnosidase from Aspergillus terreus and the enzymatic conversion of rutin to isoquercitrin by adding sorbitol.

    abstract:BACKGROUND:Thermally stable α-L-rhamnosidase with cleaving terminal α-L-rhamnose activity has great potential in industrial application. Therefore, it is necessary to find a proper method to improve the thermal stability of α-L-rhamnosidase. RESULTS:In this study, addition of sorbitol has been found to increase the th...

    journal_title:BMC biotechnology

    pub_type: 杂志文章


    authors: Ge L,Chen A,Pei J,Zhao L,Fang X,Ding G,Wang Z,Xiao W,Tang F

    更新日期:2017-02-27 00:00:00

  • Production of recombinant VP1-derived virus-like particles from novel human polyomaviruses in yeast.

    abstract:BACKGROUND:Eleven new human polyomaviruses (HPyVs) have been identified in the last decade. Serological studies show that these novel HPyVs sub-clinically infect humans at an early age. The routes of infection, entry pathways, and cell tropism of new HPyVs remain unknown. VP1 proteins of polyomaviruses can assembly int...

    journal_title:BMC biotechnology

    pub_type: 杂志文章


    authors: Norkiene M,Stonyte J,Ziogiene D,Mazeike E,Sasnauskas K,Gedvilaite A

    更新日期:2015-08-04 00:00:00

  • High-efficiency protein expression in plants from agroinfection-compatible Tobacco mosaic virus expression vectors.

    abstract:BACKGROUND:Plants are increasingly being examined as alternative recombinant protein expression systems. Recombinant protein expression levels in plants from Tobacco mosaic virus (TMV)-based vectors are much higher than those possible from plant promoters. However the common TMV expression vectors are costly, and at ti...

    journal_title:BMC biotechnology

    pub_type: 杂志文章


    authors: Lindbo JA

    更新日期:2007-08-27 00:00:00

  • Dynamic in vivo imaging and cell tracking using a histone fluorescent protein fusion in mice.

    abstract:BACKGROUND:Advances in optical imaging modalities and the continued evolution of genetically-encoded fluorescent proteins are coming together to facilitate the study of cell behavior at high resolution in living organisms. As a result, imaging using autofluorescent protein reporters is gaining popularity in mouse trans...

    journal_title:BMC biotechnology

    pub_type: 杂志文章


    authors: Hadjantonakis AK,Papaioannou VE

    更新日期:2004-12-24 00:00:00

  • Selection of oleaginous yeasts for fatty acid production.

    abstract:BACKGROUND:Oleaginous yeast species are an alternative for the production of lipids or triacylglycerides (TAGs). These yeasts are usually non-pathogenic and able to store TAGs ranging from 20 % to 70 % of their cell mass depending on culture conditions. TAGs originating from oleaginous yeasts can be used as the so-call...

    journal_title:BMC biotechnology

    pub_type: 杂志文章


    authors: Lamers D,van Biezen N,Martens D,Peters L,van de Zilver E,Jacobs-van Dreumel N,Wijffels RH,Lokman C

    更新日期:2016-05-27 00:00:00

  • Vaccination with recombinant Boophilus annulatus Bm86 ortholog protein, Ba86, protects cattle against B. annulatus and B. microplus infestations.

    abstract:BACKGROUND:The cattle ticks, Boophilus spp., affect cattle production in tropical and subtropical regions of the world. Tick vaccines constitute a cost-effective and environmentally friendly alternative to tick control. The recombinant B. microplus Bm86 protective antigen has been shown to protect cattle against tick i...

    journal_title:BMC biotechnology

    pub_type: 杂志文章


    authors: Canales M,Almazán C,Naranjo V,Jongejan F,de la Fuente J

    更新日期:2009-03-31 00:00:00

  • Stable high-level expression of factor VIII in Chinese hamster ovary cells in improved elongation factor-1 alpha-based system.

    abstract:BACKGROUND:Recombinant factor VIII (FVIII), used for haemophilia A therapy, is one of the most challenging among the therapeutic proteins produced in heterologous expression systems. Deletion variant of FVIII, in which the entire domain B is replaced by a short linker peptide, was approved for medical use. Efficacy and...

    journal_title:BMC biotechnology

    pub_type: 杂志文章


    authors: Orlova NA,Kovnir SV,Gabibov AG,Vorobiev II

    更新日期:2017-03-24 00:00:00

  • Heterologous expression, purification and characterization of nitrilase from Aspergillus niger K10.

    abstract:BACKGROUND:Nitrilases attract increasing attention due to their utility in the mild hydrolysis of nitriles. According to activity and gene screening, filamentous fungi are a rich source of nitrilases distinct in evolution from their widely examined bacterial counterparts. However, fungal nitrilases have been less explo...

    journal_title:BMC biotechnology

    pub_type: 杂志文章,收录出版


    authors: Kaplan O,Bezouška K,Plíhal O,Ettrich R,Kulik N,Vaněk O,Kavan D,Benada O,Malandra A,Sveda O,Veselá AB,Rinágelová A,Slámová K,Cantarella M,Felsberg J,Dušková J,Dohnálek J,Kotik M,Křen V,Martínková L

    更新日期:2011-01-06 00:00:00

  • Preparation of a novel monoclonal antibody against caprine interleukin-17A and its applications in immunofluorescence and immunohistochemistry assays.

    abstract:BACKGROUND:Interleukin-17 (IL-17), the characteristic cytokine secreted by T helper 17 lymphocytes (Th17 cells), plays a pivotal role in host defense and many inflammatory or autoimmune diseases. The aim of this study was to obtain purified protein caprine IL-17A (cIL-17A) as an antigen for preparing an IL-17A-specific...

    journal_title:BMC biotechnology

    pub_type: 杂志文章


    authors: Gao Y,Sang FF,Meng L,Wang Y,Ma WT,Chen K

    更新日期:2019-07-17 00:00:00

  • Reliable handling of highly A/T-rich genomic DNA for efficient generation of knockin strains of Dictyostelium discoideum.

    abstract:BACKGROUND:Social amoeba, Dictyostelium discoideum, is a well-established model organism for studying cellular physiology and developmental pattern formation. Its haploid genome facilitates functional analysis of genes by a single round of mutagenesis including targeted disruption. Although the efficient generation of ...

    journal_title:BMC biotechnology

    pub_type: 杂志文章


    authors: Mukai A,Ichiraku A,Horikawa K

    更新日期:2016-04-14 00:00:00

  • The trade-off of availability and growth inhibition through copper for the production of copper-dependent enzymes by Pichia pastoris.

    abstract:BACKGROUND:Copper is an essential chemical element for life as it is a part of prosthetic groups of enzymes including super oxide dismutase and cytochrome c oxidase; however, it is also toxic at high concentrations. Here, we present the trade-off of copper availability and growth inhibition of a common host used for co...

    journal_title:BMC biotechnology

    pub_type: 杂志文章


    authors: Balakumaran PA,Förster J,Zimmermann M,Charumathi J,Schmitz A,Czarnotta E,Lehnen M,Sudarsan S,Ebert BE,Blank LM,Meenakshisundaram S

    更新日期:2016-02-20 00:00:00

  • Isolation of anti-toxin single domain antibodies from a semi-synthetic spiny dogfish shark display library.

    abstract:BACKGROUND:Shark heavy chain antibody, also called new antigen receptor (NAR), consists of one single Variable domain (VH), containing only two complementarity-determining regions (CDRs). The antigen binding affinity and specificity are mainly determined by these two CDRs. The good solubility, excellent thermal stabili...

    journal_title:BMC biotechnology

    pub_type: 杂志文章


    authors: Liu JL,Anderson GP,Goldman ER

    更新日期:2007-11-19 00:00:00

  • Using quantitative real-time PCR to detect chimeras in transgenic tobacco and apricot and to monitor their dissociation.

    abstract:BACKGROUND:The routine generation of transgenic plants involves analysis of transgene integration into the host genome by means of Southern blotting. However, this technique cannot distinguish between uniformly transformed tissues and the presence of a mixture of transgenic and non-transgenic cells in the same tissue. ...

    journal_title:BMC biotechnology

    pub_type: 杂志文章


    authors: Faize M,Faize L,Burgos L

    更新日期:2010-07-16 00:00:00

  • A miniaturized sandwich immunoassay platform for the detection of protein-protein interactions.

    abstract:BACKGROUND:Analysis of protein-protein interactions (PPIs) is a valuable approach for the characterization of huge networks of protein complexes or proteins of unknown function. Co-immunoprecipitation (coIP) using affinity resins coupled to protein A/G is the most widely used method for PPI detection. However, this tra...

    journal_title:BMC biotechnology

    pub_type: 杂志文章


    authors: Liu Q,Chen Q,Wang J,Zhang Y,Zhou Y,Lin C,He W,He F,Xu D

    更新日期:2010-10-28 00:00:00

  • Association of high pressure and alkaline condition for solubilization of inclusion bodies and refolding of the NS1 protein from zika virus.

    abstract:BACKGROUND:Proteins in inclusion bodies (IBs) present native-like secondary structures. However, chaotropic agents at denaturing concentrations, which are widely used for IB solubilization and subsequent refolding, unfold these secondary structures. Removal of the chaotropes frequently causes reaggregation and poor rec...

    journal_title:BMC biotechnology

    pub_type: 杂志文章


    authors: Rosa da Silva CM,Chura-Chambi RM,Ramos Pereira L,Cordeiro Y,de Souza Ferreira LC,Morganti L

    更新日期:2018-12-12 00:00:00