Oxaliplatin long-circulating liposomes improved therapeutic index of colorectal carcinoma.

abstract：BACKGROUND:von Willebrand factor (VWF) is a key load bearing domain for mamalian cell adhesion by binding various macromolecular ligands in extracellular matrix such as, collagens, elastin, and glycosaminoglycans. Interestingly, vWF like domains are also commonly found in load bearing systems of marine organisms such a...

journal_title：BMC biotechnology

authors： Yoo HY,Song YH,Foo M,Seo E,Hwang DS,Seo JH

更新日期：2016-02-16 00:00:00

abstract：BACKGROUND:Hydrophobic polystyrene is the most common material for solid phase immunoassay. Proteins are immobilized on polystyrene by passive adsorption, which often causes considerable denaturation. Biological macromolecules were found to better retain their functional activity when immobilized on hydrophilic materia...

journal_title：BMC biotechnology

authors： Shmanai VV,Nikolayeva TA,Vinokurova LG,Litoshka AA

更新日期：2001-01-01 00:00:00

abstract：BACKGROUND:The photorespiratory nitrogen cycle in C₃ plants involves an extensive diversion of carbon and nitrogen away from the direct pathways of assimilation. The liberated ammonia is re-assimilated, but up to 25% of the carbon may be released into the atmosphere as CO₂. Because of the loss of CO₂ and high energy co...

journal_title：BMC biotechnology

authors： Carvalho Jde F,Madgwick PJ,Powers SJ,Keys AJ,Lea PJ,Parry MA

更新日期：2011-11-21 00:00:00

abstract：BACKGROUND:In recent years, cold-active esterases have received increased attention due to their attractive properties for some industrial applications such as high catalytic activity at low temperatures. RESULTS:An esterase-encoding gene (estS, 909 bp) from Serratia sp. was identified, cloned and expressed in Escheri...

journal_title：BMC biotechnology

authors： Jiang H,Zhang S,Gao H,Hu N

更新日期：2016-01-22 00:00:00

abstract：BACKGROUND:The routine generation of transgenic plants involves analysis of transgene integration into the host genome by means of Southern blotting. However, this technique cannot distinguish between uniformly transformed tissues and the presence of a mixture of transgenic and non-transgenic cells in the same tissue. ...

journal_title：BMC biotechnology

authors： Faize M,Faize L,Burgos L

更新日期：2010-07-16 00:00:00

abstract：BACKGROUND:Quantum dots (QDs) have been considered as a new and efficient probe for labeling cells non-invasively in vitro and in vivo, but fairly little is known about how QDs are eliminated from cells after labeling. The purpose of this study is to investigate the metabolism of QDs in different type of cells. RESULT...

journal_title：BMC biotechnology

authors： Pi QM,Zhang WJ,Zhou GD,Liu W,Cao Y

更新日期：2010-05-06 00:00:00

abstract：BACKGROUND:Xenoantigens are a major source of concern with regard to the success of interspecific xenografts. GGTA1 encodes α1,3-galactosyltransferase, which is essential for the biosynthesis of galactosyl-alpha 1,3-galactose, the major xenoantigen causing hyperacute rejection. GGTA1-modified pigs, therefore, are promi...

journal_title：BMC biotechnology

authors： Tanihara F,Hirata M,Nguyen NT,Sawamoto O,Kikuchi T,Doi M,Otoi T

更新日期：2020-08-18 00:00:00

abstract：BACKGROUND:We have investigated the possibility and feasibility of producing the HPV-11 L1 major capsid protein in transgenic Arabidopsis thaliana ecotype Columbia and Nicotiana tabacum cv. Xanthi as potential sources for an inexpensive subunit vaccine. RESULTS:Transformation of plants was only achieved with the HPV-1...

journal_title：BMC biotechnology

authors： Kohl TO,Hitzeroth II,Christensen ND,Rybicki EP

更新日期：2007-09-12 00:00:00

abstract：BACKGROUND:Asian rust (Phakopsora pachyrhizi) is a common disease in Brazilian soybean fields and it is difficult to control. To identify a biochemical candidate with potential to combat this disease, a new chitinase-like xylanase inhibitor protein (XIP) from coffee (Coffea arabica) (CaclXIP) leaves was cloned into the...

journal_title：BMC biotechnology

authors： Vasconcelos EA,Santana CG,Godoy CV,Seixas CD,Silva MS,Moreira LR,Oliveira-Neto OB,Price D,Fitches E,Filho EX,Mehta A,Gatehouse JA,Grossi-De-Sa MF

更新日期：2011-02-07 00:00:00

abstract：BACKGROUND:Ligand-targeted approaches have proven successful in improving the therapeutic index of a number of drugs. We hypothesized that the specific targeting of TNF-alpha antagonists to inflamed tissues could increase drug efficacy and reduce side effects. RESULTS:Using uteroglobin (UG), a potent anti-inflammatory...

journal_title：BMC biotechnology

authors： Ventura E,Balza E,Borsi L,Tutolo G,Carnemolla B,Castellani P,Zardi L

更新日期：2011-11-10 00:00:00

abstract：BACKGROUND:Cell-penetrating peptides (CPPs) have been widely used as carriers to transport different molecules into living cells, whereas messenger RNAs (mRNAs) have been utilized as target molecules for the prevention and treatment of various diseases. However, the instability of CPPs and mRNAs has limited their appli...

journal_title：BMC biotechnology

authors： Sun Y,Sun Y,Zhao R,Gao K

更新日期：2016-05-28 00:00:00

abstract：BACKGROUND:There is an increasing need to understand cell-cell interactions for cell and tissue engineering purposes, such as optimizing cell sheet constructs, as well as for examining adhesion defect diseases. For cell-sheet engineering, one major obstacle to sheet function is that cell sheets in suspension are fragil...

journal_title：BMC biotechnology

authors： Wei Q,Reidler D,Shen MY,Huang H

更新日期：2013-02-26 00:00:00

abstract：BACKGROUND:There is an imperative necessity for alternative sources of energy able to reduce the world dependence of fossil oil. One of the most successful options is ethanol obtained mainly from sugarcane and corn fermentation. The foremost residue from sugarcane industry is the bagasse, a rich lignocellulosic raw mat...

journal_title：BMC biotechnology

authors： Robl D,Delabona Pda S,Mergel CM,Rojas JD,Costa Pdos S,Pimentel IC,Vicente VA,da Cruz Pradella JG,Padilla G

更新日期：2013-10-31 00:00:00

abstract：BACKGROUND:Methylated resveratrol analogs show similar biological activities that are comparable with those of the resveratrol. However, the methylated resveratrol analogs exhibit better bioavailability as they are more easily transported into the cell and more resistant to degradation. Although these compounds are wid...

journal_title：BMC biotechnology

authors： Kang SY,Lee JK,Choi O,Kim CY,Jang JH,Hwang BY,Hong YS

更新日期：2014-07-17 00:00:00

abstract：BACKGROUND:Proteins in inclusion bodies (IBs) present native-like secondary structures. However, chaotropic agents at denaturing concentrations, which are widely used for IB solubilization and subsequent refolding, unfold these secondary structures. Removal of the chaotropes frequently causes reaggregation and poor rec...

journal_title：BMC biotechnology

authors： Rosa da Silva CM,Chura-Chambi RM,Ramos Pereira L,Cordeiro Y,de Souza Ferreira LC,Morganti L

更新日期：2018-12-12 00:00:00

abstract：BACKGROUND:Transformed cells of Escherichia coli DH5-alpha with pGFPuv, induced by IPTG (isopropyl-beta-d-thiogalactopyranoside), express the green fluorescent protein (gfpuv) during growth phases. E. coli subjected to the combination of selective permeation by freezing/thawing/sonication cycles followed by the three-p...

journal_title：BMC biotechnology

authors： Vessoni Penna TC,Ishii M

更新日期：2002-04-24 00:00:00

abstract：BACKGROUND:Smoking and tobacco use continue to be the largest preventable causes of death globally. A novel therapeutic approach has recently been proposed: administration of an enzyme that degrades nicotine, the main addictive component of tobacco, minimizing brain exposure and reducing its reinforcing effects. Pre-cl...

journal_title：BMC biotechnology

authors： Thisted T,Biesova Z,Walmacq C,Stone E,Rodnick-Smith M,Ahmed SS,Horrigan SK,Van Engelen B,Reed C,Kalnik MW

更新日期：2019-08-02 00:00:00

abstract：BACKGROUND:The recent H1N1 influenza pandemic illustrated the shortcomings of the vaccine manufacturing process. The A/California/07/2009 H1N1 pandemic influenza vaccine or A(H1N1)pdm09 was available late and in short supply as a result of delays in production caused by low yields and poor antigen stability. Recombinan...

journal_title：BMC biotechnology

authors： Feshchenko E,Rhodes DG,Felberbaum R,McPherson C,Rininger JA,Post P,Cox MM

更新日期：2012-10-30 00:00:00

abstract：BACKGROUND:The ubiquitin protein is present in all eukaryotic cells and promoters from ubiquitin genes are good candidates to regulate the constitutive expression of transgenes in plants. Therefore, two switchgrass (Panicum virgatum L.) ubiquitin genes (PvUbi1 and PvUbi2) were cloned and characterized. Reporter constru...

journal_title：BMC biotechnology

authors： Mann DG,King ZR,Liu W,Joyce BL,Percifield RJ,Hawkins JS,LaFayette PR,Artelt BJ,Burris JN,Mazarei M,Bennetzen JL,Parrott WA,Stewart CN Jr

更新日期：2011-07-11 00:00:00

abstract：BACKGROUND:Heat-stable antifungal factor (HSAF) is a newly identified broad-spectrum antifungal antibiotic from the biocontrol agent Lysobacter enzymogenes and is regarded as a potential biological pesticide, due to its novel mode of action. However, the production level of HSAF is quite low, and little research has re...

journal_title：BMC biotechnology

authors： Tang B,Sun C,Zhao Y,Xu H,Xu G,Liu F

更新日期：2018-10-24 00:00:00

abstract：BACKGROUND:Polysialic acid (polySia) is a carbohydrate modification of the neural cell adhesion molecule (NCAM), which is implicated in neural differentiation and plays an important role in tumor development and metastasis. Polysialylation of NCAM is mediated by two Golgi-resident polysialyltransferases (polyST) ST8Sia...

journal_title：BMC biotechnology

authors： Somplatzki S,Mühlenhoff M,Kröger A,Gerardy-Schahn R,Böldicke T

更新日期：2017-05-12 00:00:00

abstract：BACKGROUND:The therapeutic use of α-amidated peptides (e.g. calcitonin, glucagon-like peptide) has increased dramatically, but there are major impediments to wider use of such peptides. Larger peptides are expensive to synthesize, and short plasma half-lives frequently limit the clinical circumstances in which the pept...

journal_title：BMC biotechnology

authors： Carlson KR,Pomerantz SC,Li J,Vafa O,Naso M,Strohl W,Mains RE,Eipper BA

更新日期：2015-06-27 00:00:00

abstract：BACKGROUND:Plants may represent excellent alternatives to classical heterologous protein expression systems, especially for the production of biopharmaceuticals and vaccine components. Modern vaccines are becoming increasingly complex, with the incorporation of multiple antigens. Approaches towards developing an HIV va...

journal_title：BMC biotechnology

authors： Marusic C,Nuttall J,Buriani G,Lico C,Lombardi R,Baschieri S,Benvenuto E,Frigerio L

更新日期：2007-02-26 00:00:00

abstract：BACKGROUND:MicroRNAs are important regulators of gene expression at the post-transcriptional level and play an important role in many biological processes. Due to the important biological role it is of great interest to quantitatively determine their expression level in different biological settings. RESULTS:We descri...

journal_title：BMC biotechnology

authors： Balcells I,Cirera S,Busk PK

更新日期：2011-06-25 00:00:00

abstract：BACKGROUND:Aldehyde-deformylating oxygenase (ADO) is a key enzyme involved in the biosynthetic pathway of fatty alk(a/e)nes in cyanobacteria. However, cADO (cyanobacterial ADO) showed extreme low activity with the k cat value below 1 min-1, which would limit its application in biofuel production. To identify the activi...

journal_title：BMC biotechnology

authors： Wang Q,Bao L,Jia C,Li M,Li JJ,Lu X

更新日期：2017-03-16 00:00:00

abstract：BACKGROUND:The identification of known mutations in a cell population is important for clinical applications and basic cancer research. In this work an immobilized form of the polymerase chain reaction, referred to as polony technology, was used to detect mutations as well as gene deletions, resulting in loss of hetero...

journal_title：BMC biotechnology

authors： Butz J,Wickstrom E,Edwards J

更新日期：2003-07-23 00:00:00

abstract：BACKGROUND:In the previous study, the cellulolytic Escherichia coli ZH-4 isolated from bovine rumen was found to show extracellular cellulase activity and could degrade cellulose in the culture. The goal of this work was to identify and characterize the secreted cellulase of E. coli ZH-4. It will be helpful to re-under...

journal_title：BMC biotechnology

authors： Pang J,Wang J,Liu Z,Zhang Q,Qi Q

更新日期：2019-08-27 00:00:00

abstract：BACKGROUND:Heparan sulfate glycosaminoglycans are diverse components of certain proteoglycans and are known to interact with growth factors as a co-receptor necessary to induce signalling and growth factor activity. In this report we characterize heterogeneously glycosylated recombinant human perlecan domain 1 (HSPG2 a...

journal_title：BMC biotechnology

authors： Ellis AL,Pan W,Yang G,Jones K,Chuang C,Whitelock JM,DeCarlo AA

更新日期：2010-09-09 00:00:00

abstract：BACKGROUND:Despite positive reports on the efficacy of stem cell therapy for the treatment of cardiovascular disease, the nature of stem cell homing to ischemic tissues remains elusive. RESULTS:We used a mouse model of peripheral tissue ischemia to study the survival and homing capacity of dual reporter gene (eGFP/Luc...

journal_title：BMC biotechnology

authors： Everaert BR,Bergwerf I,De Vocht N,Ponsaerts P,Van Der Linden A,Timmermans JP,Vrints CJ

更新日期：2012-12-03 00:00:00

abstract：BACKGROUND:Plants are increasingly being examined as alternative recombinant protein expression systems. Recombinant protein expression levels in plants from Tobacco mosaic virus (TMV)-based vectors are much higher than those possible from plant promoters. However the common TMV expression vectors are costly, and at ti...

journal_title：BMC biotechnology

authors： Lindbo JA

更新日期：2007-08-27 00:00:00