Ligation-anchored PCR unveils immune repertoire of TCR-beta from whole blood.

abstract：BACKGROUND:The proteomics literature has seen a proliferation of publications that seek to apply the rapidly improving technology of 2D gels to study various biological systems. However, there is a dearth of systematic studies that have investigated appropriate statistical approaches to analyse the data from these expe...

journal_title：BMC biotechnology

authors： Meleth S,Deshane J,Kim H

更新日期：2005-02-11 00:00:00

abstract：BACKGROUND:Oleaginous yeast species are an alternative for the production of lipids or triacylglycerides (TAGs). These yeasts are usually non-pathogenic and able to store TAGs ranging from 20 % to 70 % of their cell mass depending on culture conditions. TAGs originating from oleaginous yeasts can be used as the so-call...

journal_title：BMC biotechnology

authors： Lamers D,van Biezen N,Martens D,Peters L,van de Zilver E,Jacobs-van Dreumel N,Wijffels RH,Lokman C

更新日期：2016-05-27 00:00:00

abstract：BACKGROUND:Shark heavy chain antibody, also called new antigen receptor (NAR), consists of one single Variable domain (VH), containing only two complementarity-determining regions (CDRs). The antigen binding affinity and specificity are mainly determined by these two CDRs. The good solubility, excellent thermal stabili...

journal_title：BMC biotechnology

authors： Liu JL,Anderson GP,Goldman ER

更新日期：2007-11-19 00:00:00

abstract：BACKGROUND:Understanding and manipulating gene function in physiological conditions is a major objective for both fundamental and applied research. In contrast to other experimental settings, which use either purely genetic or gene delivery (viral or non-viral) strategies, we report here a strategy based on direct prot...

journal_title：BMC biotechnology

authors： Gitton Y,Tibaldi L,Dupont E,Levi G,Joliot A

更新日期：2009-04-24 00:00:00

abstract：BACKGROUND:Rapid, robust and reversible induction of transgene expression would significantly facilitate cancer gene therapy as well as allow the in vivo functional study of newly discovered genes in tumor formation and progression. The popularity of the ecdysone inducible gene switch system has led us to investigate w...

journal_title：BMC biotechnology

authors： Karns LR,Kisielewski A,Gulding KM,Seraj JM,Theodorescu D

更新日期：2001-01-01 00:00:00

abstract：BACKGROUND:Eleven new human polyomaviruses (HPyVs) have been identified in the last decade. Serological studies show that these novel HPyVs sub-clinically infect humans at an early age. The routes of infection, entry pathways, and cell tropism of new HPyVs remain unknown. VP1 proteins of polyomaviruses can assembly int...

journal_title：BMC biotechnology

authors： Norkiene M,Stonyte J,Ziogiene D,Mazeike E,Sasnauskas K,Gedvilaite A

更新日期：2015-08-04 00:00:00

abstract：BACKGROUND:Fungal laccases are multicopper oxidases with huge applicability in different sectors. Here, we describe the development of a set of high-throughput colorimetric assays for screening laccase libraries in directed evolution studies. RESULTS:Firstly, we designed three colorimetric assays based on the oxidatio...

journal_title：BMC biotechnology

authors： Pardo I,Chanagá X,Vicente AI,Alcalde M,Camarero S

更新日期：2013-10-26 00:00:00

abstract：BACKGROUND:Microarrays are powerful tools for DNA-based molecular diagnostics and identification of pathogens. Most target a limited range of organisms and are based on only one or a very few genes for specific identification. Such microarrays are limited to organisms for which specific probes are available, and often ...

journal_title：BMC biotechnology

authors： Pasquer F,Pelludat C,Duffy B,Frey JE

更新日期：2010-02-17 00:00:00

abstract：BACKGROUND:The isothermal amplification of RNA in vitro has been used for the study of in vitro evolution of RNA. Although Qβ replicase has been traditionally used as an enzyme for this purpose, we planned to use norovirus replicase (NV3D(pol)) due to its structural simplicity in the scope of in vitro autonomous evolut...

journal_title：BMC biotechnology

authors： Arai H,Nishigaki K,Nemoto N,Suzuki M,Husimi Y

更新日期：2013-10-09 00:00:00

abstract：BACKGROUND:Quantum dots (QDs) have been considered as a new and efficient probe for labeling cells non-invasively in vitro and in vivo, but fairly little is known about how QDs are eliminated from cells after labeling. The purpose of this study is to investigate the metabolism of QDs in different type of cells. RESULT...

journal_title：BMC biotechnology

authors： Pi QM,Zhang WJ,Zhou GD,Liu W,Cao Y

更新日期：2010-05-06 00:00:00

abstract：BACKGROUND:Gene therapy continues to hold great potential for treating many different types of disease and dysfunction. Safe and efficient techniques for gene transfer and expression in vivo are needed to enable gene therapeutic strategies to be effective in patients. Currently, the most commonly used methods employ re...

journal_title：BMC biotechnology

authors： Xia J,Martinez A,Daniell H,Ebert SN

更新日期：2011-06-02 00:00:00

abstract：BACKGROUND:The insect cell line is a critical component in the production of recombinant proteins in the baculovirus expression system and new cell lines hold the promise of increasing both quantity and quality of protein production. RESULTS:Seventy cell lines were established by single-cell cloning from a primary cul...

journal_title：BMC biotechnology

authors： Hashimoto Y,Zhang S,Blissard GW

更新日期：2010-07-05 00:00:00

abstract：BACKGROUND:Spinal cord injury (SCI) remains a huge medical problem nowadays as there is no hospital providing the versatile strategies for repairing central nervous system and restoring function. Herein, we focused on PC-12 cells as an important research tool and studied the potential role of resveratrol (RSV) in infla...

journal_title：BMC biotechnology

authors： Zhang G,Liu Y,Xu L,Sha C,Zhang H,Xu W

更新日期：2019-01-28 00:00:00

abstract：BACKGROUND:At first glance, there appears to be a high degree of similarity between the metabolism of yeast (the Crabtree effect) and human cancer cells (the Warburg effect). At the root of both effects is accelerated metabolic flow through glycolysis which leads to overflows of ethanol and lactic acid, respectively. I...

journal_title：BMC biotechnology

authors： Andrejc D,Možir A,Legiša M

更新日期：2017-05-08 00:00:00

abstract：BACKGROUND:The safety of mutagenized and genetically transformed plants remains a subject of scrutiny. Data gathered and communicated on the phenotypic and molecular variation induced by gene transfer technologies will provide a scientific-based means to rationally address such concerns. In this study, genomic structur...

journal_title：BMC biotechnology

authors： Anderson JE,Michno JM,Kono TJ,Stec AO,Campbell BW,Curtin SJ,Stupar RM

更新日期：2016-05-12 00:00:00

abstract：BACKGROUND:Each year, influenza is responsible for hundreds of thousand cases of illness and deaths worldwide. Due to the virus' fast mutation rate, the World Health Organization (WHO) is constantly on alert to rapidly respond to emerging pandemic strains. Although anti-viral therapies exist, the most proficient way to...

journal_title：BMC biotechnology

authors： Thompson CM,Petiot E,Mullick A,Aucoin MG,Henry O,Kamen AA

更新日期：2015-05-16 00:00:00

abstract：BACKGROUND:The use of highly reproducible and spatiallyhomogeneous thin film matrices permits automated microscopy and quantitative determination of the response of hundreds of cells in a population. Using thin films of extracellular matrix proteins, we have quantified, on a cell-by-cell basis, phenotypic parameters of...

journal_title：BMC biotechnology

authors： Langenbach KJ,Elliott JT,Tona A,McDaniel D,Plant AL

更新日期：2006-03-06 00:00:00

abstract：BACKGROUND:Over-expressed native or recombinant proteins are commonly used for industrial and pharmaceutical purposes, as well as for research. Proteins of interest need to be purified in sufficient quantity, quality and specific activity to justify their commercial price and eventual medical use. Proteome quality was ...

journal_title：BMC biotechnology

authors： Musa M,Radman M,Krisko A

更新日期：2016-03-11 00:00:00

abstract：BACKGROUND:Taxol is an effective antineoplastic agent, originally extracted from the bark of Taxus brevifolia with a low yield. Many attempts have been made to produce Taxol by chemical synthesis, semi-synthesis and plant tissue cultures. However, to date, the availability of this compound is not sufficient to satisfy ...

journal_title：BMC biotechnology

authors： Bestoso F,Ottaggio L,Armirotti A,Balbi A,Damonte G,Degan P,Mazzei M,Cavalli F,Ledda B,Miele M

更新日期：2006-12-06 00:00:00

abstract：BACKGROUND:Transformed cells of Escherichia coli DH5-alpha with pGFPuv, induced by IPTG (isopropyl-beta-d-thiogalactopyranoside), express the green fluorescent protein (gfpuv) during growth phases. E. coli subjected to the combination of selective permeation by freezing/thawing/sonication cycles followed by the three-p...

journal_title：BMC biotechnology

authors： Vessoni Penna TC,Ishii M

更新日期：2002-04-24 00:00:00

abstract：BACKGROUND:Anti-NMDA receptor encephalitis (ANRE) is a potentially lethal disease attributed to auto-antibodies against the N-methyl-D-aspartate receptor (NMDAR). Full recovery is possible if therapy is initiated early in the disease course. Detection of ANRE antibodies in the cerebrospinal fluid (CSF) is essential for...

journal_title：BMC biotechnology

authors： Sharma R,Al-Saleem FH,Puligedda RD,Rattelle A,Lynch DR,Dessain SK

更新日期：2018-06-27 00:00:00

abstract：BACKGROUND:Vascular endothelial growth factor (VEGF) is a key angiogenic factors. It plays an important role in both physiologic and pathologic angiogenesis and increases permeability across the vessels. Using antibody phage display technology, we obtained a novel anti-VEGFA IgG, named as FD006. In this study, the phar...

journal_title：BMC biotechnology

authors： Wang Q,Yang J,Tang K,Luo L,Wang L,Tian L,Jiang Y,Feng J,Li Y,Shen B,Lv M,Huang Y

更新日期：2014-02-27 00:00:00

abstract：BACKGROUND:The CRISPR/Cas9 system is increasingly used for gene inactivation in mouse zygotes, but homology-directed mutagenesis and use of inbred embryos are less established. In particular, Rosa26 knock-in alleles for the insertion of transgenes in a genomic 'safe harbor' site, have not been produced. Here we applied...

journal_title：BMC biotechnology

authors： Chu VT,Weber T,Graf R,Sommermann T,Petsch K,Sack U,Volchkov P,Rajewsky K,Kühn R

更新日期：2016-01-16 00:00:00

abstract：BACKGROUND:There are significant differences in the culture conditions between small-scale screenings and large-scale fermentation processes. Production processes are usually conducted in fed-batch cultivation mode with active pH-monitoring and control. In contrast, screening experiments in shake flasks are usually con...

journal_title：BMC biotechnology

authors： Scheidle M,Dittrich B,Klinger J,Ikeda H,Klee D,Büchs J

更新日期：2011-03-23 00:00:00

abstract：: ...

journal_title：BMC biotechnology

authors： Devilder MC,Moyon M,Gautreau-Rolland L,Navet B,Perroteau J,Delbos F,Gesnel MC,Breathnach R,Saulquin X

更新日期：2019-02-18 00:00:00

abstract：BACKGROUND:In view of compliance with increasingly stringent environmental legislation, an eco-friendly treatment technology of industrial dyes and effluents is a major environmental challenge in the color industry. In present study, a promising and eco-friendly entrapment approach was adopted to immobilize purified ma...

journal_title：BMC biotechnology

authors： Bilal M,Asgher M

更新日期：2015-12-10 00:00:00

abstract：BACKGROUND:In the last three decades, the Baculovirus expression vector system (BEV) has evolved to one of the most widely used eukaryotic systems for heterologous protein expression including approved vaccines and therapies. Despite the significant improvements introduced during the past years, the BEV system still ha...

journal_title：BMC biotechnology

authors： Scholz J,Suppmann S

更新日期：2017-11-16 00:00:00

abstract：BACKGROUND:Polymerase chain reaction (PCR) is one of the most important developments in modern biotechnology. However, PCR is known to introduce biases, especially during multiplex reactions. Recent studies have implicated the DNA polymerase as the primary source of bias, particularly initiation of polymerization on th...

journal_title：BMC biotechnology

authors： Pan W,Byrne-Steele M,Wang C,Lu S,Clemmons S,Zahorchak RJ,Han J

更新日期：2014-01-30 00:00:00

abstract：BACKGROUND:Multi-copy gene integration into microbial genomes is a conventional tool for obtaining improved gene expression. For Penicillium chrysogenum, the fungal producer of the beta-lactam antibiotic penicillin, many production strains carry multiple copies of the penicillin biosynthesis gene cluster. This discover...

journal_title：BMC biotechnology

authors： Ziemons S,Koutsantas K,Becker K,Dahlmann T,Kück U

更新日期：2017-02-16 00:00:00

abstract：BACKGROUND:Engineered proteins, with non-immunoglobulin scaffolds, have become an important alternative to antibodies in many biotechnical and therapeutic applications. When compared to antibodies, tailored proteins may provide advantageous properties such as a smaller size or a more stable structure. RESULTS:Avidin i...

journal_title：BMC biotechnology

authors： Riihimäki TA,Hiltunen S,Rangl M,Nordlund HR,Määttä JA,Ebner A,Hinterdorfer P,Kulomaa MS,Takkinen K,Hytönen VP

更新日期：2011-06-09 00:00:00