Thin films of Type 1 collagen for cell by cell analysis of morphology and tenascin-C promoter activity.

abstract：BACKGROUND:Quantum dots (QDs) have been considered as a new and efficient probe for labeling cells non-invasively in vitro and in vivo, but fairly little is known about how QDs are eliminated from cells after labeling. The purpose of this study is to investigate the metabolism of QDs in different type of cells. RESULT...

journal_title：BMC biotechnology

authors： Pi QM,Zhang WJ,Zhou GD,Liu W,Cao Y

更新日期：2010-05-06 00:00:00

abstract：BACKGROUND:This study aims to assess suitability of hydroponic technology for treatment of brewery wastewater in a hydroponic bioreactor using Typha latifolia. Triplicated hydroponic bioreactor treatment units were designed, constructed and operated at a hydraulic retention time of 5 days with different surface loading...

journal_title：BMC biotechnology

authors： Gebeyehu A,Shebeshe N,Kloos H,Belay S

更新日期：2018-11-22 00:00:00

abstract：BACKGROUND:CRISPR/Cas9 is widely used for precise genetic editing in various organisms. CRISPR/Cas9 editing may in many plants be hampered by the presence of complex and high ploidy genomes and inefficient or poorly controlled delivery of the CRISPR/Cas9 components to gamete cells or cells with regenerative potential. ...

journal_title：BMC biotechnology

authors： Petersen BL,Möller SR,Mravec J,Jørgensen B,Christensen M,Liu Y,Wandall HH,Bennett EP,Yang Z

更新日期：2019-06-17 00:00:00

abstract：BACKGROUND:Recombinant factor VIII (FVIII), used for haemophilia A therapy, is one of the most challenging among the therapeutic proteins produced in heterologous expression systems. Deletion variant of FVIII, in which the entire domain B is replaced by a short linker peptide, was approved for medical use. Efficacy and...

journal_title：BMC biotechnology

authors： Orlova NA,Kovnir SV,Gabibov AG,Vorobiev II

更新日期：2017-03-24 00:00:00

abstract：BACKGROUND:Expressed Sequence Tag (EST) sequences are widely used in applications such as genome annotation, gene discovery and gene expression studies. However, some of GenBank dbEST sequences have proven to be "unclean". Identification of cDNA termini/ends and their structures in raw ESTs not only facilitates data qu...

journal_title：BMC biotechnology

authors： Zhou S,Ji G,Liu X,Li P,Moler J,Karro JE,Liang C

更新日期：2012-05-03 00:00:00

abstract：BACKGROUND:Cymbidium faberi is one of the oldest cultivars of oriental orchids, with an elegant flower fragrance. In order to investigate the molecular mechanism and the functions of related proteins in the methyl jasmonate (MeJA) signaling pathway, one of the main components of flower fragrance in C. faberi, yeast one...

journal_title：BMC biotechnology

authors： Xu Y,Zhou J,Liu Q,Li K,Zhou Y

更新日期：2020-01-16 00:00:00

abstract：BACKGROUND:Genetically encoded sensors developed on the basis of green fluorescent protein (GFP)-like proteins are becoming more and more popular instruments for monitoring cellular analytes and enzyme activities in living cells and transgenic organisms. In particular, a number of Ca2+ sensors have been developed, eith...

journal_title：BMC biotechnology

authors： Souslova EA,Belousov VV,Lock JG,Strömblad S,Kasparov S,Bolshakov AP,Pinelis VG,Labas YA,Lukyanov S,Mayr LM,Chudakov DM

更新日期：2007-06-29 00:00:00

abstract：BACKGROUND:The ubiquitin protein is present in all eukaryotic cells and promoters from ubiquitin genes are good candidates to regulate the constitutive expression of transgenes in plants. Therefore, two switchgrass (Panicum virgatum L.) ubiquitin genes (PvUbi1 and PvUbi2) were cloned and characterized. Reporter constru...

journal_title：BMC biotechnology

authors： Mann DG,King ZR,Liu W,Joyce BL,Percifield RJ,Hawkins JS,LaFayette PR,Artelt BJ,Burris JN,Mazarei M,Bennetzen JL,Parrott WA,Stewart CN Jr

更新日期：2011-07-11 00:00:00

abstract：BACKGROUND:Acetyl-xylan esterase (AXE, EC 3.1.1.72) hydrolyses acetate group from the linear chain of xylopyranose residues bound by β-1,4-linkage. The enzyme finds commercial applications in bio-bleaching of wood pulp, treating animal feed to increase digestibility, processing food to increase clarification and conver...

journal_title：BMC biotechnology

authors： Atta S,Ali S,Akhtar MN,Haq I

更新日期：2011-05-16 00:00:00

abstract：BACKGROUND:In view of compliance with increasingly stringent environmental legislation, an eco-friendly treatment technology of industrial dyes and effluents is a major environmental challenge in the color industry. In present study, a promising and eco-friendly entrapment approach was adopted to immobilize purified ma...

journal_title：BMC biotechnology

authors： Bilal M,Asgher M

更新日期：2015-12-10 00:00:00

abstract：BACKGROUND:Each year, influenza is responsible for hundreds of thousand cases of illness and deaths worldwide. Due to the virus' fast mutation rate, the World Health Organization (WHO) is constantly on alert to rapidly respond to emerging pandemic strains. Although anti-viral therapies exist, the most proficient way to...

journal_title：BMC biotechnology

authors： Thompson CM,Petiot E,Mullick A,Aucoin MG,Henry O,Kamen AA

更新日期：2015-05-16 00:00:00

abstract：BACKGROUND:Engineered proteins, with non-immunoglobulin scaffolds, have become an important alternative to antibodies in many biotechnical and therapeutic applications. When compared to antibodies, tailored proteins may provide advantageous properties such as a smaller size or a more stable structure. RESULTS:Avidin i...

journal_title：BMC biotechnology

authors： Riihimäki TA,Hiltunen S,Rangl M,Nordlund HR,Määttä JA,Ebner A,Hinterdorfer P,Kulomaa MS,Takkinen K,Hytönen VP

更新日期：2011-06-09 00:00:00

abstract：BACKGROUND:Methylated CpG dinucleotides in promoters are associated with the loss of gene expression in recombinant Chinese hamster ovary (CHO) cells during large-scale commercial manufacturing. We evaluated a promoter devoid of CpG dinucleotides, CpGfree, in parallel with a similar CpG containing promoter, CpGrich, fo...

journal_title：BMC biotechnology

authors： Ho SC,Koh EY,Soo BP,Mariati,Chao SH,Yang Y

更新日期：2016-10-18 00:00:00

abstract：BACKGROUND:The isothermal amplification of RNA in vitro has been used for the study of in vitro evolution of RNA. Although Qβ replicase has been traditionally used as an enzyme for this purpose, we planned to use norovirus replicase (NV3D(pol)) due to its structural simplicity in the scope of in vitro autonomous evolut...

journal_title：BMC biotechnology

authors： Arai H,Nishigaki K,Nemoto N,Suzuki M,Husimi Y

更新日期：2013-10-09 00:00:00

abstract：BACKGROUND:The ability of cytosine deaminase (CD) to convert the antifungal agent 5-fluorocytosine (5-FC) into one of the most potent and largely used anticancer compound such as 5-fluorouracil (5-FU) raised considerable interest in this enzyme to model gene or antibody - directed enzyme-prodrug therapy (GDEPT/ADEPT) a...

journal_title：BMC biotechnology

authors： Mallano A,Zamboni S,Carpinelli G,Santoro F,Flego M,Ascione A,Gellini M,Tombesi M,Podo F,Cianfriglia M

更新日期：2008-09-10 00:00:00

abstract：BACKGROUND:Cytotoxic drugs are non-selective between normal and pathological tissue, and this poses a challenge regarding the strategy for treatment of tumors. To achieve sufficient antitumor activity for colorectal carcinoma, optimization of the therapeutic regimen is of great importance. We investigated the ability o...

journal_title：BMC biotechnology

authors： Yang C,Liu HZ,Fu ZX,Lu WD

更新日期：2011-03-15 00:00:00

abstract：BACKGROUND:The ability to produce the same recombinant protein in both prokaryotic and eukaryotic cells offers many experimental opportunities. However, the cloning of the same gene into multiple plasmids is required, which is time consuming, laborious and still may not produce soluble, stable protein in sufficient qua...

journal_title：BMC biotechnology

authors： Sinah N,Williams CA,Piper RC,Shields SB

更新日期：2012-08-23 00:00:00

abstract：BACKGROUND:The CRISPR/Cas9 genome editing system has greatly facilitated and expanded our capacity to engineer mammalian genomes, including targeted gene knock-outs. However, the phenotyping of the knock-out effect requires a high DNA editing efficiency. RESULTS:Here, we report a user-friendly strategy based on the ex...

journal_title：BMC biotechnology

authors： Liesche C,Venkatraman L,Aschenbrenner S,Grosse S,Grimm D,Eils R,Beaudouin J

更新日期：2016-02-16 00:00:00

abstract：BACKGROUND:The insect cell line is a critical component in the production of recombinant proteins in the baculovirus expression system and new cell lines hold the promise of increasing both quantity and quality of protein production. RESULTS:Seventy cell lines were established by single-cell cloning from a primary cul...

journal_title：BMC biotechnology

authors： Hashimoto Y,Zhang S,Blissard GW

更新日期：2010-07-05 00:00:00

abstract：BACKGROUND:Alkaline phosphatase (AP) catalyzes the hydrolytic cleavage of phosphate monoesters under alkaline conditions and plays important roles in microbial ecology and molecular biology applications. Here, we report on the first isolation and biochemical characterization of a thermolabile AP from a metagenome. RES...

journal_title：BMC biotechnology

authors： Lee DH,Choi SL,Rha E,Kim SJ,Yeom SJ,Moon JH,Lee SG

更新日期：2015-01-31 00:00:00

abstract：BACKGROUND:Serine hydroxymethyltransferase (SHMT) is the key enzyme in L-serine enzymatic production, suggesting the importance of obtaining a SHMT with high activity. RESULTS:Here, a novel SHMT gene, glyA, was obtained through degenerate oligonucleotide-primed PCR and encoded a novel SHMT with 54.3% similarity to the...

journal_title：BMC biotechnology

authors： Jiang W,Chen L,Hu N,Yuan S,Li B,Liu Z

更新日期：2014-11-14 00:00:00

abstract：BACKGROUND:Heparan sulfate glycosaminoglycans are diverse components of certain proteoglycans and are known to interact with growth factors as a co-receptor necessary to induce signalling and growth factor activity. In this report we characterize heterogeneously glycosylated recombinant human perlecan domain 1 (HSPG2 a...

journal_title：BMC biotechnology

authors： Ellis AL,Pan W,Yang G,Jones K,Chuang C,Whitelock JM,DeCarlo AA

更新日期：2010-09-09 00:00:00

abstract：BACKGROUND:The proteomics literature has seen a proliferation of publications that seek to apply the rapidly improving technology of 2D gels to study various biological systems. However, there is a dearth of systematic studies that have investigated appropriate statistical approaches to analyse the data from these expe...

journal_title：BMC biotechnology

authors： Meleth S,Deshane J,Kim H

更新日期：2005-02-11 00:00:00

abstract：BACKGROUND:Fungal laccases are multicopper oxidases with huge applicability in different sectors. Here, we describe the development of a set of high-throughput colorimetric assays for screening laccase libraries in directed evolution studies. RESULTS:Firstly, we designed three colorimetric assays based on the oxidatio...

journal_title：BMC biotechnology

authors： Pardo I,Chanagá X,Vicente AI,Alcalde M,Camarero S

更新日期：2013-10-26 00:00:00

abstract：BACKGROUND:The use of small interfering RNA (siRNA) molecules in animals to achieve double-stranded RNA-mediated interference (RNAi) has recently emerged as a powerful method of sequence-specific gene knockdown. As DNA-based expression of short hairpin RNA (shRNA) for RNAi may offer some advantages over chemical and in...

journal_title：BMC biotechnology

authors： Lambeth LS,Moore RJ,Muralitharan M,Dalrymple BP,McWilliam S,Doran TJ

更新日期：2005-05-11 00:00:00

abstract：BACKGROUND:With the increasing number of GMOs on the global market the maintenance of European GMO regulations is becoming more complex. For the analysis of a single food or feed sample it is necessary to assess the sample for the presence of many GMO-targets simultaneously at a sensitive level. Several methods have be...

journal_title：BMC biotechnology

authors： Ujhelyi G,Dijk JP,Prins TW,Voorhuijzen MM,Hoef AM,Beenen HG,Morisset D,Gruden K,Kok EJ

更新日期：2012-01-19 00:00:00

abstract：BACKGROUND:Multi-copy gene integration into microbial genomes is a conventional tool for obtaining improved gene expression. For Penicillium chrysogenum, the fungal producer of the beta-lactam antibiotic penicillin, many production strains carry multiple copies of the penicillin biosynthesis gene cluster. This discover...

journal_title：BMC biotechnology

authors： Ziemons S,Koutsantas K,Becker K,Dahlmann T,Kück U

更新日期：2017-02-16 00:00:00

abstract：: ...

journal_title：BMC biotechnology

authors： Devilder MC,Moyon M,Gautreau-Rolland L,Navet B,Perroteau J,Delbos F,Gesnel MC,Breathnach R,Saulquin X

更新日期：2019-02-18 00:00:00

abstract：BACKGROUND:In recent years, cold-active esterases have received increased attention due to their attractive properties for some industrial applications such as high catalytic activity at low temperatures. RESULTS:An esterase-encoding gene (estS, 909 bp) from Serratia sp. was identified, cloned and expressed in Escheri...

journal_title：BMC biotechnology

authors： Jiang H,Zhang S,Gao H,Hu N

更新日期：2016-01-22 00:00:00

abstract：BACKGROUND:Loop mediated isothermal amplification of nucleic acid templates is a rapid, sensitive and specific method suitable for molecular diagnostics. However the complexity of primer design and the number of primers involved can lead to false positives from non-specific primer interactions. Standard methods of LAMP...

journal_title：BMC biotechnology

authors： Hardinge P,Murray JAH

更新日期：2019-08-01 00:00:00