Efficient generation of Rosa26 knock-in mice using CRISPR/Cas9 in C57BL/6 zygotes.

abstract：BACKGROUND:Genetically encoded sensors developed on the basis of green fluorescent protein (GFP)-like proteins are becoming more and more popular instruments for monitoring cellular analytes and enzyme activities in living cells and transgenic organisms. In particular, a number of Ca2+ sensors have been developed, eith...

journal_title：BMC biotechnology

authors： Souslova EA,Belousov VV,Lock JG,Strömblad S,Kasparov S,Bolshakov AP,Pinelis VG,Labas YA,Lukyanov S,Mayr LM,Chudakov DM

更新日期：2007-06-29 00:00:00

abstract：BACKGROUND:Transformed cells of Escherichia coli DH5-alpha with pGFPuv, induced by IPTG (isopropyl-beta-d-thiogalactopyranoside), express the green fluorescent protein (gfpuv) during growth phases. E. coli subjected to the combination of selective permeation by freezing/thawing/sonication cycles followed by the three-p...

journal_title：BMC biotechnology

authors： Vessoni Penna TC,Ishii M

更新日期：2002-04-24 00:00:00

abstract：BACKGROUND:With the increasing number of GMOs on the global market the maintenance of European GMO regulations is becoming more complex. For the analysis of a single food or feed sample it is necessary to assess the sample for the presence of many GMO-targets simultaneously at a sensitive level. Several methods have be...

journal_title：BMC biotechnology

authors： Ujhelyi G,Dijk JP,Prins TW,Voorhuijzen MM,Hoef AM,Beenen HG,Morisset D,Gruden K,Kok EJ

更新日期：2012-01-19 00:00:00

abstract：BACKGROUND:As one of the genetic mechanisms for adaptive immunity, V(D)J recombination generates an enormous repertoire of T-cell receptors (TCRs). With the development of high-throughput sequencing techniques, systematic exploration of V(D)J recombination becomes possible. Multiplex PCR has been previously developed t...

journal_title：BMC biotechnology

authors： Gao F,Wang K

更新日期：2015-05-28 00:00:00

abstract：BACKGROUND:Serine hydroxymethyltransferase (SHMT) is the key enzyme in L-serine enzymatic production, suggesting the importance of obtaining a SHMT with high activity. RESULTS:Here, a novel SHMT gene, glyA, was obtained through degenerate oligonucleotide-primed PCR and encoded a novel SHMT with 54.3% similarity to the...

journal_title：BMC biotechnology

authors： Jiang W,Chen L,Hu N,Yuan S,Li B,Liu Z

更新日期：2014-11-14 00:00:00

abstract：BACKGROUND:Copper is an essential chemical element for life as it is a part of prosthetic groups of enzymes including super oxide dismutase and cytochrome c oxidase; however, it is also toxic at high concentrations. Here, we present the trade-off of copper availability and growth inhibition of a common host used for co...

journal_title：BMC biotechnology

authors： Balakumaran PA,Förster J,Zimmermann M,Charumathi J,Schmitz A,Czarnotta E,Lehnen M,Sudarsan S,Ebert BE,Blank LM,Meenakshisundaram S

更新日期：2016-02-20 00:00:00

abstract：BACKGROUND:Fungal laccases are multicopper oxidases with huge applicability in different sectors. Here, we describe the development of a set of high-throughput colorimetric assays for screening laccase libraries in directed evolution studies. RESULTS:Firstly, we designed three colorimetric assays based on the oxidatio...

journal_title：BMC biotechnology

authors： Pardo I,Chanagá X,Vicente AI,Alcalde M,Camarero S

更新日期：2013-10-26 00:00:00

abstract：BACKGROUND:Interest in using plants for production of recombinant proteins such as monoclonal antibodies is growing, but proteolytic degradation, leading to a loss of functionality and complications in downstream purification, is still a serious problem. RESULTS:In this study, we investigated the dynamics of the assem...

journal_title：BMC biotechnology

authors： Hehle VK,Paul MJ,Drake PM,Ma JK,van Dolleweerd CJ

更新日期：2011-12-30 00:00:00

abstract：BACKGROUND:CRISPR/Cas9 is widely used for precise genetic editing in various organisms. CRISPR/Cas9 editing may in many plants be hampered by the presence of complex and high ploidy genomes and inefficient or poorly controlled delivery of the CRISPR/Cas9 components to gamete cells or cells with regenerative potential. ...

journal_title：BMC biotechnology

authors： Petersen BL,Möller SR,Mravec J,Jørgensen B,Christensen M,Liu Y,Wandall HH,Bennett EP,Yang Z

更新日期：2019-06-17 00:00:00

abstract：BACKGROUND:Analogues of vitamin D3 are extensively used in the treatment of various illnesses, such as osteoporosis, inflammatory skin diseases, and cancer. Functional testing of new vitamin D3 analogues and formulations for improved systemic and topical administration is supported by sensitive screening methods that a...

journal_title：BMC biotechnology

authors： Staunstrup NH,Sharma N,Bak RO,Svensson L,Petersen TK,Aarenstrup L,Kristiansen K,Bolund L,Mikkelsen JG

更新日期：2011-04-07 00:00:00

abstract：BACKGROUND:There is an increasing need to understand cell-cell interactions for cell and tissue engineering purposes, such as optimizing cell sheet constructs, as well as for examining adhesion defect diseases. For cell-sheet engineering, one major obstacle to sheet function is that cell sheets in suspension are fragil...

journal_title：BMC biotechnology

authors： Wei Q,Reidler D,Shen MY,Huang H

更新日期：2013-02-26 00:00:00

abstract：BACKGROUND:Despite positive reports on the efficacy of stem cell therapy for the treatment of cardiovascular disease, the nature of stem cell homing to ischemic tissues remains elusive. RESULTS:We used a mouse model of peripheral tissue ischemia to study the survival and homing capacity of dual reporter gene (eGFP/Luc...

journal_title：BMC biotechnology

authors： Everaert BR,Bergwerf I,De Vocht N,Ponsaerts P,Van Der Linden A,Timmermans JP,Vrints CJ

更新日期：2012-12-03 00:00:00

abstract：BACKGROUND:Loop mediated isothermal amplification of nucleic acid templates is a rapid, sensitive and specific method suitable for molecular diagnostics. However the complexity of primer design and the number of primers involved can lead to false positives from non-specific primer interactions. Standard methods of LAMP...

journal_title：BMC biotechnology

authors： Hardinge P,Murray JAH

更新日期：2019-08-01 00:00:00

abstract：BACKGROUND:In recent years, cold-active esterases have received increased attention due to their attractive properties for some industrial applications such as high catalytic activity at low temperatures. RESULTS:An esterase-encoding gene (estS, 909 bp) from Serratia sp. was identified, cloned and expressed in Escheri...

journal_title：BMC biotechnology

authors： Jiang H,Zhang S,Gao H,Hu N

更新日期：2016-01-22 00:00:00

abstract：BACKGROUND:L-ornithine is effective in the treatment of liver diseases and helps strengthen the heart. The commercial applications mean that efficient biotechnological production of L-ornithine has become increasingly necessary. Adaptive evolution strategies have been proven a feasible and efficient technique to achiev...

journal_title：BMC biotechnology

authors： Jiang LY,Chen SG,Zhang YY,Liu JZ

更新日期：2013-06-01 00:00:00

abstract：BACKGROUND:Recombinant factor VIII (FVIII), used for haemophilia A therapy, is one of the most challenging among the therapeutic proteins produced in heterologous expression systems. Deletion variant of FVIII, in which the entire domain B is replaced by a short linker peptide, was approved for medical use. Efficacy and...

journal_title：BMC biotechnology

authors： Orlova NA,Kovnir SV,Gabibov AG,Vorobiev II

更新日期：2017-03-24 00:00:00

abstract：BACKGROUND:The extracellular domain of matrix protein 2 (M2e) of influenza A virus is a promising target for the development of a universal vaccine against influenza because M2e sequences are highly conserved among human influenza A strains. However, native M2e is poorly immunogenic, but its immunogenicity can be incre...

journal_title：BMC biotechnology

authors： Mardanova ES,Kotlyarov RY,Kuprianov VV,Stepanova LA,Tsybalova LM,Lomonosoff GP,Ravin NV

更新日期：2015-05-29 00:00:00

abstract：BACKGROUND:The safety of mutagenized and genetically transformed plants remains a subject of scrutiny. Data gathered and communicated on the phenotypic and molecular variation induced by gene transfer technologies will provide a scientific-based means to rationally address such concerns. In this study, genomic structur...

journal_title：BMC biotechnology

authors： Anderson JE,Michno JM,Kono TJ,Stec AO,Campbell BW,Curtin SJ,Stupar RM

更新日期：2016-05-12 00:00:00

abstract：BACKGROUND:Clustered Regularly Interspaced Short Palindromic Repeats/CRISPR associated protein 9 (CRISPR/Cas9) has been wildly used to generate gene knockout models through inducing indels causing frame-shift. However, there are few studies concerning the post-transcript effects caused by CRISPR-mediated genome editing...

journal_title：BMC biotechnology

authors： Tang JX,Chen D,Deng SL,Li J,Li Y,Fu Z,Wang XX,Zhang Y,Chen SR,Liu YX

更新日期：2018-10-03 00:00:00

abstract：BACKGROUND:Polymerase chain reaction (PCR) is one of the most important developments in modern biotechnology. However, PCR is known to introduce biases, especially during multiplex reactions. Recent studies have implicated the DNA polymerase as the primary source of bias, particularly initiation of polymerization on th...

journal_title：BMC biotechnology

authors： Pan W,Byrne-Steele M,Wang C,Lu S,Clemmons S,Zahorchak RJ,Han J

更新日期：2014-01-30 00:00:00

abstract：BACKGROUND:The use of small interfering RNA (siRNA) molecules in animals to achieve double-stranded RNA-mediated interference (RNAi) has recently emerged as a powerful method of sequence-specific gene knockdown. As DNA-based expression of short hairpin RNA (shRNA) for RNAi may offer some advantages over chemical and in...

journal_title：BMC biotechnology

authors： Lambeth LS,Moore RJ,Muralitharan M,Dalrymple BP,McWilliam S,Doran TJ

更新日期：2005-05-11 00:00:00

abstract：BACKGROUND:The CRISPR-Cas9 system is a powerful and versatile tool for crop genome editing. However, achieving highly efficient and specific editing in polyploid species can be a challenge. The efficiency and specificity of the CRISPR-Cas9 system depends critically on the gRNA used. Here, we assessed the activities and...

journal_title：BMC biotechnology

authors： Arndell T,Sharma N,Langridge P,Baumann U,Watson-Haigh NS,Whitford R

更新日期：2019-10-30 00:00:00

abstract：BACKGROUND:This study aims to assess suitability of hydroponic technology for treatment of brewery wastewater in a hydroponic bioreactor using Typha latifolia. Triplicated hydroponic bioreactor treatment units were designed, constructed and operated at a hydraulic retention time of 5 days with different surface loading...

journal_title：BMC biotechnology

authors： Gebeyehu A,Shebeshe N,Kloos H,Belay S

更新日期：2018-11-22 00:00:00

abstract：BACKGROUND:Methylated resveratrol analogs show similar biological activities that are comparable with those of the resveratrol. However, the methylated resveratrol analogs exhibit better bioavailability as they are more easily transported into the cell and more resistant to degradation. Although these compounds are wid...

journal_title：BMC biotechnology

authors： Kang SY,Lee JK,Choi O,Kim CY,Jang JH,Hwang BY,Hong YS

更新日期：2014-07-17 00:00:00

abstract：BACKGROUND:Dissolved oxygen tension (DOT) is hardly constant and homogenously distributed in a bioreactor, which can have a negative impact in the metabolism and product synthesis. However, the effects of DOT on plasmid DNA (pDNA) production and quality have not been thoroughly investigated. In the present study, the e...

journal_title：BMC biotechnology

authors： Jaén KE,Sigala JC,Olivares-Hernández R,Niehaus K,Lara AR

更新日期：2017-07-04 00:00:00

abstract：BACKGROUND:The ability to produce the same recombinant protein in both prokaryotic and eukaryotic cells offers many experimental opportunities. However, the cloning of the same gene into multiple plasmids is required, which is time consuming, laborious and still may not produce soluble, stable protein in sufficient qua...

journal_title：BMC biotechnology

authors： Sinah N,Williams CA,Piper RC,Shields SB

更新日期：2012-08-23 00:00:00

abstract：BACKGROUND:Cytotoxic drugs are non-selective between normal and pathological tissue, and this poses a challenge regarding the strategy for treatment of tumors. To achieve sufficient antitumor activity for colorectal carcinoma, optimization of the therapeutic regimen is of great importance. We investigated the ability o...

journal_title：BMC biotechnology

authors： Yang C,Liu HZ,Fu ZX,Lu WD

更新日期：2011-03-15 00:00:00

abstract：BACKGROUND:Heat-stable antifungal factor (HSAF) is a newly identified broad-spectrum antifungal antibiotic from the biocontrol agent Lysobacter enzymogenes and is regarded as a potential biological pesticide, due to its novel mode of action. However, the production level of HSAF is quite low, and little research has re...

journal_title：BMC biotechnology

authors： Tang B,Sun C,Zhao Y,Xu H,Xu G,Liu F

更新日期：2018-10-24 00:00:00

abstract：BACKGROUND:The ubiquitin protein is present in all eukaryotic cells and promoters from ubiquitin genes are good candidates to regulate the constitutive expression of transgenes in plants. Therefore, two switchgrass (Panicum virgatum L.) ubiquitin genes (PvUbi1 and PvUbi2) were cloned and characterized. Reporter constru...

journal_title：BMC biotechnology

authors： Mann DG,King ZR,Liu W,Joyce BL,Percifield RJ,Hawkins JS,LaFayette PR,Artelt BJ,Burris JN,Mazarei M,Bennetzen JL,Parrott WA,Stewart CN Jr

更新日期：2011-07-11 00:00:00

abstract：BACKGROUND:Urease B is an important virulence factor that is required for Helicobacter pylori to colonise the gastric mucosa. Mouse monoclonal antibodies (mAbs) that inhibit urease B enzymatic activity will be useful as vaccines for the prevention and treatment of H. pylori infection. Here, we produced murine mAbs agai...

journal_title：BMC biotechnology

authors： Li Y,Ning Y,Wang Y,Peng D,Jiang Y,Zhang L,Long M,Luo J,Li M

更新日期：2010-11-30 00:00:00