Degradation or excretion of quantum dots in mouse embryonic stem cells.

abstract：BACKGROUND:DsRed the red fluorescent protein (RFP) isolated from Discosoma sp. coral holds much promise as a genetically and spectrally distinct alternative to green fluorescent protein (GFP) for application in mice. Widespread use of DsRed has been hampered by several issues resulting in the inability to establish and...

journal_title：BMC biotechnology

authors： Long JZ,Lackan CS,Hadjantonakis AK

更新日期：2005-07-04 00:00:00

abstract：BACKGROUND:Clostridial co-culture containing cellulolytic and solventogenic species is a potential consolidated bioprocessing (CBP) approach for producing biochemicals and biofuels from cellulosic biomass. It has been demonstrated that the rate of cellulose utilization in the co-culture of Clostridium acetobutylicum an...

journal_title：BMC biotechnology

authors： Salimi F,Mahadevan R

更新日期：2013-11-04 00:00:00

abstract：BACKGROUND:Interleukin-17 (IL-17), the characteristic cytokine secreted by T helper 17 lymphocytes (Th17 cells), plays a pivotal role in host defense and many inflammatory or autoimmune diseases. The aim of this study was to obtain purified protein caprine IL-17A (cIL-17A) as an antigen for preparing an IL-17A-specific...

journal_title：BMC biotechnology

authors： Gao Y,Sang FF,Meng L,Wang Y,Ma WT,Chen K

更新日期：2019-07-17 00:00:00

abstract：BACKGROUND:In the previous study, the cellulolytic Escherichia coli ZH-4 isolated from bovine rumen was found to show extracellular cellulase activity and could degrade cellulose in the culture. The goal of this work was to identify and characterize the secreted cellulase of E. coli ZH-4. It will be helpful to re-under...

journal_title：BMC biotechnology

authors： Pang J,Wang J,Liu Z,Zhang Q,Qi Q

更新日期：2019-08-27 00:00:00

abstract：: ...

journal_title：BMC biotechnology

authors： Devilder MC,Moyon M,Gautreau-Rolland L,Navet B,Perroteau J,Delbos F,Gesnel MC,Breathnach R,Saulquin X

更新日期：2019-02-18 00:00:00

abstract：BACKGROUND:In a previous study, anthocyanin levels in potato plants were increased by manipulating genes connected with the flavonoid biosynthesis pathway. However, starch content and tuber yield were dramatically reduced in the transgenic plants, which over-expressed dihydroflavonol reductase (DFR). RESULTS:Transgeni...

journal_title：BMC biotechnology

authors： Aksamit-Stachurska A,Korobczak-Sosna A,Kulma A,Szopa J

更新日期：2008-03-05 00:00:00

abstract：BACKGROUND:One of the most commonly used vectors for gene therapy is the adenoviral vector; its ability to tightly regulate transgene expression is critical for optimizing therapeutic outcomes. The tetracycline-regulated system (especially the Tet-On system) for gene expression is one of the most valuable tools for con...

journal_title：BMC biotechnology

authors： Chen H,Wang D,Xia R,Mao Q,Xia H

更新日期：2015-02-13 00:00:00

abstract：BACKGROUND:Xylanase can replace chemical additives to improve the volume and sensory properties of bread in the baking. Suitable baking xylanase with improved yield will promote the application of xylanase in baking industry. The xylanase XYNZG from the Plectosphaerella cucumerina has been previously characterized by h...

journal_title：BMC biotechnology

authors： Zhan FX,Wang QH,Jiang SJ,Zhou YL,Zhang GM,Ma YH

更新日期：2014-12-16 00:00:00

abstract：BACKGROUND:The isothermal amplification of RNA in vitro has been used for the study of in vitro evolution of RNA. Although Qβ replicase has been traditionally used as an enzyme for this purpose, we planned to use norovirus replicase (NV3D(pol)) due to its structural simplicity in the scope of in vitro autonomous evolut...

journal_title：BMC biotechnology

authors： Arai H,Nishigaki K,Nemoto N,Suzuki M,Husimi Y

更新日期：2013-10-09 00:00:00

abstract：BACKGROUND:The detection of unknown mutations is important in research and medicine. For this purpose, a mismatch-specific endonuclease CEL I from celery has been established as a useful tool in high throughput projects. Previously, CEL I-like activities were described only in a variety of plants and could not be expre...

journal_title：BMC biotechnology

authors： Pimkin M,Caretti E,Canutescu A,Yeung JB,Cohn H,Chen Y,Oleykowski C,Bellacosa A,Yeung AT

更新日期：2007-06-01 00:00:00

abstract：BACKGROUND:In vivo use of monoclonal antibodies has become routine clinical practice in the treatment of human cancer. CD38 is an attractive target, because it has double roles, as a receptor and an ectoenzyme. Daratumumab, an anti-CD38 antibody, is currently in the clinical trials for multiple myeloma. RESULTS:Here w...

journal_title：BMC biotechnology

authors： Yu T,Qiao C,Lv M,Tang L

更新日期：2019-05-22 00:00:00

abstract：BACKGROUND:Dissolved oxygen tension (DOT) is hardly constant and homogenously distributed in a bioreactor, which can have a negative impact in the metabolism and product synthesis. However, the effects of DOT on plasmid DNA (pDNA) production and quality have not been thoroughly investigated. In the present study, the e...

journal_title：BMC biotechnology

authors： Jaén KE,Sigala JC,Olivares-Hernández R,Niehaus K,Lara AR

更新日期：2017-07-04 00:00:00

abstract：BACKGROUND:Thermally stable α-L-rhamnosidase with cleaving terminal α-L-rhamnose activity has great potential in industrial application. Therefore, it is necessary to find a proper method to improve the thermal stability of α-L-rhamnosidase. RESULTS:In this study, addition of sorbitol has been found to increase the th...

journal_title：BMC biotechnology

authors： Ge L,Chen A,Pei J,Zhao L,Fang X,Ding G,Wang Z,Xiao W,Tang F

更新日期：2017-02-27 00:00:00

abstract：BACKGROUND:Mesenchymal stem cells (MSCs) are of great interest in bone regenerative medicine due to their osteogenic potential and trophic effects. However, challenges to large-scale production of MSCs can hinder the translation of MSC therapies. 3D Microcarrier (MC)-based MSC culture presents a scalable and cost-effec...

journal_title：BMC biotechnology

authors： Shekaran A,Sim E,Tan KY,Chan JK,Choolani M,Reuveny S,Oh S

更新日期：2015-10-31 00:00:00

abstract：BACKGROUND:Cymbidium faberi is one of the oldest cultivars of oriental orchids, with an elegant flower fragrance. In order to investigate the molecular mechanism and the functions of related proteins in the methyl jasmonate (MeJA) signaling pathway, one of the main components of flower fragrance in C. faberi, yeast one...

journal_title：BMC biotechnology

authors： Xu Y,Zhou J,Liu Q,Li K,Zhou Y

更新日期：2020-01-16 00:00:00

abstract：BACKGROUND:Wax esters are important ingredients in cosmetics, pharmaceuticals, lubricants and other chemical industries due to their excellent wetting property. Since the naturally occurring wax esters are expensive and scarce, these esters can be produced by enzymatic alcoholysis of vegetable oils. In an enzymatic rea...

journal_title：BMC biotechnology

authors： Basri M,Rahman RN,Ebrahimpour A,Salleh AB,Gunawan ER,Rahman MB

更新日期：2007-08-30 00:00:00

abstract：BACKGROUND:Nanoparticles appear to be promising devices for application in the agriculture and food industries, but information regarding the response of plants to contact with nano-devices is scarce. Toxic effects may be imposed depending on the type and concentration of nanoparticle as well as time of exposure. A num...

journal_title：BMC biotechnology

authors： Santos AR,Miguel AS,Macovei A,Maycock C,Balestrazzi A,Oliva A,Fevereiro P

更新日期：2013-12-20 00:00:00

abstract：BACKGROUND:Increasing cell membrane permeability to water and cryoprotectants is critical for the successful cryopreservation of cells with large volumes. Artificial expression of water-selective aquaporins or aquaglyceroporins (GLPs), such as mammalian aquaporin-3 (AQP3), enhances cell permeability to water and cryopr...

journal_title：BMC biotechnology

authors： Chauvigné F,Lubzens E,Cerdà J

更新日期：2011-04-08 00:00:00

abstract：BACKGROUND:We have investigated the possibility and feasibility of producing the HPV-11 L1 major capsid protein in transgenic Arabidopsis thaliana ecotype Columbia and Nicotiana tabacum cv. Xanthi as potential sources for an inexpensive subunit vaccine. RESULTS:Transformation of plants was only achieved with the HPV-1...

journal_title：BMC biotechnology

authors： Kohl TO,Hitzeroth II,Christensen ND,Rybicki EP

更新日期：2007-09-12 00:00:00

abstract：BACKGROUND:Understanding and manipulating gene function in physiological conditions is a major objective for both fundamental and applied research. In contrast to other experimental settings, which use either purely genetic or gene delivery (viral or non-viral) strategies, we report here a strategy based on direct prot...

journal_title：BMC biotechnology

authors： Gitton Y,Tibaldi L,Dupont E,Levi G,Joliot A

更新日期：2009-04-24 00:00:00

abstract：BACKGROUND:Plants are increasingly being examined as alternative recombinant protein expression systems. Recombinant protein expression levels in plants from Tobacco mosaic virus (TMV)-based vectors are much higher than those possible from plant promoters. However the common TMV expression vectors are costly, and at ti...

journal_title：BMC biotechnology

authors： Lindbo JA

更新日期：2007-08-27 00:00:00

abstract：BACKGROUND:The CRISPR-Cas9 system is a powerful and versatile tool for crop genome editing. However, achieving highly efficient and specific editing in polyploid species can be a challenge. The efficiency and specificity of the CRISPR-Cas9 system depends critically on the gRNA used. Here, we assessed the activities and...

journal_title：BMC biotechnology

authors： Arndell T,Sharma N,Langridge P,Baumann U,Watson-Haigh NS,Whitford R

更新日期：2019-10-30 00:00:00

abstract：BACKGROUND:Polysialic acid (polySia) is a carbohydrate modification of the neural cell adhesion molecule (NCAM), which is implicated in neural differentiation and plays an important role in tumor development and metastasis. Polysialylation of NCAM is mediated by two Golgi-resident polysialyltransferases (polyST) ST8Sia...

journal_title：BMC biotechnology

authors： Somplatzki S,Mühlenhoff M,Kröger A,Gerardy-Schahn R,Böldicke T

更新日期：2017-05-12 00:00:00

abstract：BACKGROUND:The insect cell line is a critical component in the production of recombinant proteins in the baculovirus expression system and new cell lines hold the promise of increasing both quantity and quality of protein production. RESULTS:Seventy cell lines were established by single-cell cloning from a primary cul...

journal_title：BMC biotechnology

authors： Hashimoto Y,Zhang S,Blissard GW

更新日期：2010-07-05 00:00:00

abstract：BACKGROUND:Analysis of protein-protein interactions (PPIs) is a valuable approach for the characterization of huge networks of protein complexes or proteins of unknown function. Co-immunoprecipitation (coIP) using affinity resins coupled to protein A/G is the most widely used method for PPI detection. However, this tra...

journal_title：BMC biotechnology

authors： Liu Q,Chen Q,Wang J,Zhang Y,Zhou Y,Lin C,He W,He F,Xu D

更新日期：2010-10-28 00:00:00

abstract：BACKGROUND:A modification of a standard method of fluorescence in situ hybridisation (FISH) is described, by which a combination of several substrates and probes on single microscope slides enables more accurate comparisons of the distribution and abundance of chromosomal sequences and improves the relatively low throu...

journal_title：BMC biotechnology

authors： Hasterok R,Dulawa J,Jenkins G,Leggett M,Langdon T

更新日期：2006-03-20 00:00:00

abstract：BACKGROUND:Microarrays are powerful tools for DNA-based molecular diagnostics and identification of pathogens. Most target a limited range of organisms and are based on only one or a very few genes for specific identification. Such microarrays are limited to organisms for which specific probes are available, and often ...

journal_title：BMC biotechnology

authors： Pasquer F,Pelludat C,Duffy B,Frey JE

更新日期：2010-02-17 00:00:00

abstract：BACKGROUND:High recombinant protein productivity in mammalian cell lines is often associated with phenotypic changes in protein content, energy metabolism, and cell growth, but the key determinants that regulate productivity are still not clearly understood. The mammalian target of rapamycin (mTOR) signalling pathway h...

journal_title：BMC biotechnology

authors： Edros R,McDonnell S,Al-Rubeai M

更新日期：2014-02-17 00:00:00

abstract：BACKGROUND:Size exclusion chromatography is the method of choice for separating free from liposome-encapsulated molecules. However, if the column is not presaturated with lipids this type of chromatography causes a significant loss of lipid material. To date, the mechanism of lipid retention is poorly understood. It ha...

journal_title：BMC biotechnology

authors： Ruysschaert T,Marque A,Duteyrat JL,Lesieur S,Winterhalter M,Fournier D

更新日期：2005-05-10 00:00:00

abstract：BACKGROUND:Establishing highly productive clonal cell lines with constant productivity over 2-3 months of continuous culture remains a tedious task requiring the screening of tens of thousands of clonal colonies. In addition, long-term cultivation of many candidate lines derived in the absence of drug selection pressur...

journal_title：BMC biotechnology

authors： Orlova NA,Kovnir SV,Hodak JA,Vorobiev II,Gabibov AG,Skryabin KG

更新日期：2014-06-14 00:00:00