Expression of HPV-11 L1 protein in transgenic Arabidopsis thaliana and Nicotiana tabacum.

abstract：BACKGROUND:Circular forms of viral genomic DNA are generated during infection of cells with retroviruses like HIV-1. Such circles are unable to replicate and are eventually lost as a result of cell division, lending support to the prevalent notion that episomal retroviral DNA forms are dead-end products of reverse tran...

journal_title：BMC biotechnology

authors： Moldt B,Staunstrup NH,Jakobsen M,Yáñez-Muñoz RJ,Mikkelsen JG

更新日期：2008-08-09 00:00:00

abstract：BACKGROUND:In vivo use of monoclonal antibodies has become routine clinical practice in the treatment of human cancer. CD38 is an attractive target, because it has double roles, as a receptor and an ectoenzyme. Daratumumab, an anti-CD38 antibody, is currently in the clinical trials for multiple myeloma. RESULTS:Here w...

journal_title：BMC biotechnology

authors： Yu T,Qiao C,Lv M,Tang L

更新日期：2019-05-22 00:00:00

abstract：BACKGROUND:Heparan sulfate glycosaminoglycans are diverse components of certain proteoglycans and are known to interact with growth factors as a co-receptor necessary to induce signalling and growth factor activity. In this report we characterize heterogeneously glycosylated recombinant human perlecan domain 1 (HSPG2 a...

journal_title：BMC biotechnology

authors： Ellis AL,Pan W,Yang G,Jones K,Chuang C,Whitelock JM,DeCarlo AA

更新日期：2010-09-09 00:00:00

abstract：BACKGROUND:Analysis of protein-protein interactions (PPIs) is a valuable approach for the characterization of huge networks of protein complexes or proteins of unknown function. Co-immunoprecipitation (coIP) using affinity resins coupled to protein A/G is the most widely used method for PPI detection. However, this tra...

journal_title：BMC biotechnology

authors： Liu Q,Chen Q,Wang J,Zhang Y,Zhou Y,Lin C,He W,He F,Xu D

更新日期：2010-10-28 00:00:00

abstract：BACKGROUND:As with many plant species, current genome editing strategies in soybean are initiated by stably transforming a gene that encodes an engineered nuclease into the genome. Expression of the transgene results in a double-stranded break and repair at the targeted locus, oftentimes resulting in mutation(s) at the...

journal_title：BMC biotechnology

authors： Michno JM,Virdi K,Stec AO,Liu J,Wang X,Xiong Y,Stupar RM

更新日期：2020-02-24 00:00:00

abstract：BACKGROUND:Cell-penetrating peptides (CPPs) have been widely used as carriers to transport different molecules into living cells, whereas messenger RNAs (mRNAs) have been utilized as target molecules for the prevention and treatment of various diseases. However, the instability of CPPs and mRNAs has limited their appli...

journal_title：BMC biotechnology

authors： Sun Y,Sun Y,Zhao R,Gao K

更新日期：2016-05-28 00:00:00

abstract：BACKGROUND:Plants may represent excellent alternatives to classical heterologous protein expression systems, especially for the production of biopharmaceuticals and vaccine components. Modern vaccines are becoming increasingly complex, with the incorporation of multiple antigens. Approaches towards developing an HIV va...

journal_title：BMC biotechnology

authors： Marusic C,Nuttall J,Buriani G,Lico C,Lombardi R,Baschieri S,Benvenuto E,Frigerio L

更新日期：2007-02-26 00:00:00

abstract：BACKGROUND:The green fluorescent protein (GFP) has been regarded as a valuable tool and widely applied as a biomarker in medical applications and diagnostics. A cost-efficient upstream expression system and an inexpensive downstream purification process will meet the demands of the GFP protein with high-purity. RESULT...

journal_title：BMC biotechnology

authors： Dong J,Ding X,Wang S

更新日期：2019-12-09 00:00:00

abstract：BACKGROUND:Human TGF-β3 has been used in many studies to induce genes coding for typical cartilage matrix components and accelerate chondrogenic differentiation, making it the standard constituent in most cultivation media used for the assessment of chondrogenesis associated with various stem cell types on carrier matr...

journal_title：BMC biotechnology

authors： Huang Y,Seitz D,Chevalier Y,Müller PE,Jansson V,Klar RM

更新日期：2020-08-27 00:00:00

abstract：BACKGROUND:The CRISPR/Cas9 genome editing system has greatly facilitated and expanded our capacity to engineer mammalian genomes, including targeted gene knock-outs. However, the phenotyping of the knock-out effect requires a high DNA editing efficiency. RESULTS:Here, we report a user-friendly strategy based on the ex...

journal_title：BMC biotechnology

authors： Liesche C,Venkatraman L,Aschenbrenner S,Grosse S,Grimm D,Eils R,Beaudouin J

更新日期：2016-02-16 00:00:00

abstract：BACKGROUND:Acetyl-xylan esterase (AXE, EC 3.1.1.72) hydrolyses acetate group from the linear chain of xylopyranose residues bound by β-1,4-linkage. The enzyme finds commercial applications in bio-bleaching of wood pulp, treating animal feed to increase digestibility, processing food to increase clarification and conver...

journal_title：BMC biotechnology

authors： Atta S,Ali S,Akhtar MN,Haq I

更新日期：2011-05-16 00:00:00

abstract：BACKGROUND:The ketoisovalerate reductase (EC 1.2.7.7 ) is required for the formation of beauvericin via the nonribosomal peptide synthetase biosynthetic pathway. It catalyzes the NADPH-specific reduction of ketoisovaleric acid to hydroxyisovalerate. However, little is known about the bioinformatics' data about the 2-Ki...

journal_title：BMC biotechnology

authors： Zhang T,Jia X,Zhuo Y,Liu M,Gao H,Liu J,Zhang L

更新日期：2012-08-23 00:00:00

abstract：BACKGROUND:Copper is an essential chemical element for life as it is a part of prosthetic groups of enzymes including super oxide dismutase and cytochrome c oxidase; however, it is also toxic at high concentrations. Here, we present the trade-off of copper availability and growth inhibition of a common host used for co...

journal_title：BMC biotechnology

authors： Balakumaran PA,Förster J,Zimmermann M,Charumathi J,Schmitz A,Czarnotta E,Lehnen M,Sudarsan S,Ebert BE,Blank LM,Meenakshisundaram S

更新日期：2016-02-20 00:00:00

abstract：BACKGROUND:Waste animal fat is a promising feedstock to replace vegetable oil that widely used in commercial biodiesel process, however the high content of free fatty acid in waste fat makes it unfeasible to be processed with commercial base-catalytic process. Enzymatic process is preferable to convert waste fat into b...

journal_title：BMC biotechnology

authors： Pollardo AA,Lee HS,Lee D,Kim S,Kim J

更新日期：2017-09-09 00:00:00

abstract：BACKGROUND:Urease B is an important virulence factor that is required for Helicobacter pylori to colonise the gastric mucosa. Mouse monoclonal antibodies (mAbs) that inhibit urease B enzymatic activity will be useful as vaccines for the prevention and treatment of H. pylori infection. Here, we produced murine mAbs agai...

journal_title：BMC biotechnology

authors： Li Y,Ning Y,Wang Y,Peng D,Jiang Y,Zhang L,Long M,Luo J,Li M

更新日期：2010-11-30 00:00:00

abstract：BACKGROUND:β-Fructofuranosidases (or invertases) catalyse the commercially-important biotransformation of sucrose into short-chain fructooligosaccharides with wide-scale application as a prebiotic in the functional foods and pharmaceutical industries. RESULTS:We identified a β-fructofuranosidase gene (CmINV) from a Ce...

journal_title：BMC biotechnology

authors： van Wyk N,Trollope KM,Steenkamp ET,Wingfield BD,Volschenk H

更新日期：2013-11-14 00:00:00

abstract：BACKGROUND:Dengue is a public health problem of global significance for which there is neither an effective antiviral therapy nor a preventive vaccine. It is a mosquito-borne viral disease, caused by dengue (DEN) viruses, which are members of the Flaviviridae family. There are four closely related serotypes, DEN-1, DEN...

journal_title：BMC biotechnology

authors： Khanam S,Rajendra P,Khanna N,Swaminathan S

更新日期：2007-02-15 00:00:00

abstract：BACKGROUND:In the last three decades, the Baculovirus expression vector system (BEV) has evolved to one of the most widely used eukaryotic systems for heterologous protein expression including approved vaccines and therapies. Despite the significant improvements introduced during the past years, the BEV system still ha...

journal_title：BMC biotechnology

authors： Scholz J,Suppmann S

更新日期：2017-11-16 00:00:00

abstract：BACKGROUND:Porcine reproductive and respiratory syndrome virus (PRRSV) causes major economic losses in the pig industry worldwide. In vivo, the virus infects a subpopulation of tissue macrophages. In vitro, PRRSV only replicates in primary pig macrophages and African green monkey kidney derived cells, such as Marc-145....

journal_title：BMC biotechnology

authors： Delrue I,Van Gorp H,Van Doorsselaere J,Delputte PL,Nauwynck HJ

更新日期：2010-06-29 00:00:00

abstract：BACKGROUND:The use of small interfering RNA (siRNA) molecules in animals to achieve double-stranded RNA-mediated interference (RNAi) has recently emerged as a powerful method of sequence-specific gene knockdown. As DNA-based expression of short hairpin RNA (shRNA) for RNAi may offer some advantages over chemical and in...

journal_title：BMC biotechnology

authors： Lambeth LS,Moore RJ,Muralitharan M,Dalrymple BP,McWilliam S,Doran TJ

更新日期：2005-05-11 00:00:00

abstract：BACKGROUND:At first glance, there appears to be a high degree of similarity between the metabolism of yeast (the Crabtree effect) and human cancer cells (the Warburg effect). At the root of both effects is accelerated metabolic flow through glycolysis which leads to overflows of ethanol and lactic acid, respectively. I...

journal_title：BMC biotechnology

authors： Andrejc D,Možir A,Legiša M

更新日期：2017-05-08 00:00:00

abstract：BACKGROUND:Taxol is an effective antineoplastic agent, originally extracted from the bark of Taxus brevifolia with a low yield. Many attempts have been made to produce Taxol by chemical synthesis, semi-synthesis and plant tissue cultures. However, to date, the availability of this compound is not sufficient to satisfy ...

journal_title：BMC biotechnology

authors： Bestoso F,Ottaggio L,Armirotti A,Balbi A,Damonte G,Degan P,Mazzei M,Cavalli F,Ledda B,Miele M

更新日期：2006-12-06 00:00:00

abstract：BACKGROUND:Shark heavy chain antibody, also called new antigen receptor (NAR), consists of one single Variable domain (VH), containing only two complementarity-determining regions (CDRs). The antigen binding affinity and specificity are mainly determined by these two CDRs. The good solubility, excellent thermal stabili...

journal_title：BMC biotechnology

authors： Liu JL,Anderson GP,Goldman ER

更新日期：2007-11-19 00:00:00

abstract：BACKGROUND:Carboxyl-terminal protease (CtpA) plays essential functions in posttranslational protein processing in prokaryotic and eukaryotic cells. To date, only a few bacterial ctpA genes have been characterized. Here we cloned and characterized a novel CtpA. The encoding gene, ctpAp (ctpA of Paenibacillus lautus), wa...

journal_title：BMC biotechnology

authors： Li Y,Pan Y,She Q,Chen L

更新日期：2013-10-25 00:00:00

abstract：BACKGROUND:Oleaginous yeast species are an alternative for the production of lipids or triacylglycerides (TAGs). These yeasts are usually non-pathogenic and able to store TAGs ranging from 20 % to 70 % of their cell mass depending on culture conditions. TAGs originating from oleaginous yeasts can be used as the so-call...

journal_title：BMC biotechnology

authors： Lamers D,van Biezen N,Martens D,Peters L,van de Zilver E,Jacobs-van Dreumel N,Wijffels RH,Lokman C

更新日期：2016-05-27 00:00:00

abstract：BACKGROUND:New high-throughput sequencing technologies promise a very sensitive and high-resolution analysis of DNA methylation patterns in quantitative terms. However, a detailed and comprehensive comparison with existing validated DNA methylation analysis methods is not yet available. Therefore, a systematic cross-va...

journal_title：BMC biotechnology

authors： Potapova A,Albat C,Hasemeier B,Haeussler K,Lamprecht S,Suerbaum S,Kreipe H,Lehmann U

更新日期：2011-01-14 00:00:00

abstract：BACKGROUND:Plants are increasingly being examined as alternative recombinant protein expression systems. Recombinant protein expression levels in plants from Tobacco mosaic virus (TMV)-based vectors are much higher than those possible from plant promoters. However the common TMV expression vectors are costly, and at ti...

journal_title：BMC biotechnology

authors： Lindbo JA

更新日期：2007-08-27 00:00:00

abstract：BACKGROUND:Quantum dots (QDs) have been considered as a new and efficient probe for labeling cells non-invasively in vitro and in vivo, but fairly little is known about how QDs are eliminated from cells after labeling. The purpose of this study is to investigate the metabolism of QDs in different type of cells. RESULT...

journal_title：BMC biotechnology

authors： Pi QM,Zhang WJ,Zhou GD,Liu W,Cao Y

更新日期：2010-05-06 00:00:00

abstract：BACKGROUND:Engineered proteins, with non-immunoglobulin scaffolds, have become an important alternative to antibodies in many biotechnical and therapeutic applications. When compared to antibodies, tailored proteins may provide advantageous properties such as a smaller size or a more stable structure. RESULTS:Avidin i...

journal_title：BMC biotechnology

authors： Riihimäki TA,Hiltunen S,Rangl M,Nordlund HR,Määttä JA,Ebner A,Hinterdorfer P,Kulomaa MS,Takkinen K,Hytönen VP

更新日期：2011-06-09 00:00:00

abstract：BACKGROUND:Interleukin-17 (IL-17), the characteristic cytokine secreted by T helper 17 lymphocytes (Th17 cells), plays a pivotal role in host defense and many inflammatory or autoimmune diseases. The aim of this study was to obtain purified protein caprine IL-17A (cIL-17A) as an antigen for preparing an IL-17A-specific...

journal_title：BMC biotechnology

authors： Gao Y,Sang FF,Meng L,Wang Y,Ma WT,Chen K

更新日期：2019-07-17 00:00:00