Similarity of recombinant human perlecan domain 1 by alternative expression systems bioactive heterogenous recombinant human perlecan D1.

abstract：BACKGROUND:The CRISPR/Cas9 system is increasingly used for gene inactivation in mouse zygotes, but homology-directed mutagenesis and use of inbred embryos are less established. In particular, Rosa26 knock-in alleles for the insertion of transgenes in a genomic 'safe harbor' site, have not been produced. Here we applied...

journal_title：BMC biotechnology

authors： Chu VT,Weber T,Graf R,Sommermann T,Petsch K,Sack U,Volchkov P,Rajewsky K,Kühn R

更新日期：2016-01-16 00:00:00

abstract：BACKGROUND:Analysis of protein-protein interactions (PPIs) is a valuable approach for the characterization of huge networks of protein complexes or proteins of unknown function. Co-immunoprecipitation (coIP) using affinity resins coupled to protein A/G is the most widely used method for PPI detection. However, this tra...

journal_title：BMC biotechnology

authors： Liu Q,Chen Q,Wang J,Zhang Y,Zhou Y,Lin C,He W,He F,Xu D

更新日期：2010-10-28 00:00:00

abstract：BACKGROUND:Microarray technology has been used extensively over the past 10 years for assessing gene expression, and has facilitated precise genetic profiling of everything from tumors to small molecule drugs. By contrast, arraying cell membranes in a manner which preserves their ability to mediate biochemical processe...

journal_title：BMC biotechnology

authors： Yamazaki V,Sirenko O,Schafer RJ,Nguyen L,Gutsmann T,Brade L,Groves JT

更新日期：2005-06-16 00:00:00

abstract：BACKGROUND:Hydrophobic polystyrene is the most common material for solid phase immunoassay. Proteins are immobilized on polystyrene by passive adsorption, which often causes considerable denaturation. Biological macromolecules were found to better retain their functional activity when immobilized on hydrophilic materia...

journal_title：BMC biotechnology

authors： Shmanai VV,Nikolayeva TA,Vinokurova LG,Litoshka AA

更新日期：2001-01-01 00:00:00

abstract：BACKGROUND:In spite of a great number of monomeric fluorescent proteins developed in the recent years, the reported fluorescent protein-based FRET pairs are still characterized by a number of disadvantageous features, complicating their use as reporters in cell biology and for high-throughput cell-based screenings. RE...

journal_title：BMC biotechnology

authors： Shcherbo D,Souslova EA,Goedhart J,Chepurnykh TV,Gaintzeva A,Shemiakina II,Gadella TW,Lukyanov S,Chudakov DM

更新日期：2009-03-25 00:00:00

abstract：BACKGROUND:The recent H1N1 influenza pandemic illustrated the shortcomings of the vaccine manufacturing process. The A/California/07/2009 H1N1 pandemic influenza vaccine or A(H1N1)pdm09 was available late and in short supply as a result of delays in production caused by low yields and poor antigen stability. Recombinan...

journal_title：BMC biotechnology

authors： Feshchenko E,Rhodes DG,Felberbaum R,McPherson C,Rininger JA,Post P,Cox MM

更新日期：2012-10-30 00:00:00

abstract：BACKGROUND:Clustered Regularly Interspaced Short Palindromic Repeats/CRISPR associated protein 9 (CRISPR/Cas9) has been wildly used to generate gene knockout models through inducing indels causing frame-shift. However, there are few studies concerning the post-transcript effects caused by CRISPR-mediated genome editing...

journal_title：BMC biotechnology

authors： Tang JX,Chen D,Deng SL,Li J,Li Y,Fu Z,Wang XX,Zhang Y,Chen SR,Liu YX

更新日期：2018-10-03 00:00:00

abstract：BACKGROUND:The antiphospholipid syndrome (APS), characterised by recurrent miscarriage and thrombosis, is a significant cause of morbidity and mortality. Domain I (DI) of human beta 2 glycoprotein I (beta2GPI) is thought to contain crucial antibody binding epitopes for antiphospholipid antibodies (aPL), which are criti...

journal_title：BMC biotechnology

authors： Ioannou Y,Giles I,Lambrianides A,Richardson C,Pearl LH,Latchman DS,Isenberg DA,Rahman A

更新日期：2006-02-10 00:00:00

abstract：BACKGROUND:With the increasing number of GMOs on the global market the maintenance of European GMO regulations is becoming more complex. For the analysis of a single food or feed sample it is necessary to assess the sample for the presence of many GMO-targets simultaneously at a sensitive level. Several methods have be...

journal_title：BMC biotechnology

authors： Ujhelyi G,Dijk JP,Prins TW,Voorhuijzen MM,Hoef AM,Beenen HG,Morisset D,Gruden K,Kok EJ

更新日期：2012-01-19 00:00:00

abstract：BACKGROUND:The ketoisovalerate reductase (EC 1.2.7.7 ) is required for the formation of beauvericin via the nonribosomal peptide synthetase biosynthetic pathway. It catalyzes the NADPH-specific reduction of ketoisovaleric acid to hydroxyisovalerate. However, little is known about the bioinformatics' data about the 2-Ki...

journal_title：BMC biotechnology

authors： Zhang T,Jia X,Zhuo Y,Liu M,Gao H,Liu J,Zhang L

更新日期：2012-08-23 00:00:00

abstract：BACKGROUND:Advances in optical imaging modalities and the continued evolution of genetically-encoded fluorescent proteins are coming together to facilitate the study of cell behavior at high resolution in living organisms. As a result, imaging using autofluorescent protein reporters is gaining popularity in mouse trans...

journal_title：BMC biotechnology

authors： Hadjantonakis AK,Papaioannou VE

更新日期：2004-12-24 00:00:00

abstract：BACKGROUND:In a previous study, anthocyanin levels in potato plants were increased by manipulating genes connected with the flavonoid biosynthesis pathway. However, starch content and tuber yield were dramatically reduced in the transgenic plants, which over-expressed dihydroflavonol reductase (DFR). RESULTS:Transgeni...

journal_title：BMC biotechnology

authors： Aksamit-Stachurska A,Korobczak-Sosna A,Kulma A,Szopa J

更新日期：2008-03-05 00:00:00

abstract：BACKGROUND:The use of highly reproducible and spatiallyhomogeneous thin film matrices permits automated microscopy and quantitative determination of the response of hundreds of cells in a population. Using thin films of extracellular matrix proteins, we have quantified, on a cell-by-cell basis, phenotypic parameters of...

journal_title：BMC biotechnology

authors： Langenbach KJ,Elliott JT,Tona A,McDaniel D,Plant AL

更新日期：2006-03-06 00:00:00

abstract：BACKGROUND:The CRISPR-Cas9 system is a powerful and versatile tool for crop genome editing. However, achieving highly efficient and specific editing in polyploid species can be a challenge. The efficiency and specificity of the CRISPR-Cas9 system depends critically on the gRNA used. Here, we assessed the activities and...

journal_title：BMC biotechnology

authors： Arndell T,Sharma N,Langridge P,Baumann U,Watson-Haigh NS,Whitford R

更新日期：2019-10-30 00:00:00

abstract：BACKGROUND:Xenoantigens are a major source of concern with regard to the success of interspecific xenografts. GGTA1 encodes α1,3-galactosyltransferase, which is essential for the biosynthesis of galactosyl-alpha 1,3-galactose, the major xenoantigen causing hyperacute rejection. GGTA1-modified pigs, therefore, are promi...

journal_title：BMC biotechnology

authors： Tanihara F,Hirata M,Nguyen NT,Sawamoto O,Kikuchi T,Doi M,Otoi T

更新日期：2020-08-18 00:00:00

abstract：BACKGROUND:The presence of coliform bacteria is routinely assessed to establish the microbiological safety of water supplies and raw or processed foods. Coliforms are a group of lactose-fermenting Enterobacteriaceae, which most likely acquired the lacZ gene by horizontal transfer and therefore constitute a polyphyletic...

journal_title：BMC biotechnology

authors： Molina F,López-Acedo E,Tabla R,Roa I,Gómez A,Rebollo JE

更新日期：2015-06-04 00:00:00

abstract：BACKGROUND:Porcine reproductive and respiratory syndrome virus (PRRSV) causes major economic losses in the pig industry worldwide. In vivo, the virus infects a subpopulation of tissue macrophages. In vitro, PRRSV only replicates in primary pig macrophages and African green monkey kidney derived cells, such as Marc-145....

journal_title：BMC biotechnology

authors： Delrue I,Van Gorp H,Van Doorsselaere J,Delputte PL,Nauwynck HJ

更新日期：2010-06-29 00:00:00

abstract：BACKGROUND:Transformed cells of Escherichia coli DH5-alpha with pGFPuv, induced by IPTG (isopropyl-beta-d-thiogalactopyranoside), express the green fluorescent protein (gfpuv) during growth phases. E. coli subjected to the combination of selective permeation by freezing/thawing/sonication cycles followed by the three-p...

journal_title：BMC biotechnology

authors： Vessoni Penna TC,Ishii M

更新日期：2002-04-24 00:00:00

abstract：BACKGROUND:New high-throughput sequencing technologies promise a very sensitive and high-resolution analysis of DNA methylation patterns in quantitative terms. However, a detailed and comprehensive comparison with existing validated DNA methylation analysis methods is not yet available. Therefore, a systematic cross-va...

journal_title：BMC biotechnology

authors： Potapova A,Albat C,Hasemeier B,Haeussler K,Lamprecht S,Suerbaum S,Kreipe H,Lehmann U

更新日期：2011-01-14 00:00:00

abstract：BACKGROUND:The identification of known mutations in a cell population is important for clinical applications and basic cancer research. In this work an immobilized form of the polymerase chain reaction, referred to as polony technology, was used to detect mutations as well as gene deletions, resulting in loss of hetero...

journal_title：BMC biotechnology

authors： Butz J,Wickstrom E,Edwards J

更新日期：2003-07-23 00:00:00

abstract：BACKGROUND:Size exclusion chromatography is the method of choice for separating free from liposome-encapsulated molecules. However, if the column is not presaturated with lipids this type of chromatography causes a significant loss of lipid material. To date, the mechanism of lipid retention is poorly understood. It ha...

journal_title：BMC biotechnology

authors： Ruysschaert T,Marque A,Duteyrat JL,Lesieur S,Winterhalter M,Fournier D

更新日期：2005-05-10 00:00:00

abstract：BACKGROUND:Waste animal fat is a promising feedstock to replace vegetable oil that widely used in commercial biodiesel process, however the high content of free fatty acid in waste fat makes it unfeasible to be processed with commercial base-catalytic process. Enzymatic process is preferable to convert waste fat into b...

journal_title：BMC biotechnology

authors： Pollardo AA,Lee HS,Lee D,Kim S,Kim J

更新日期：2017-09-09 00:00:00

abstract：BACKGROUND:Human TGF-β3 has been used in many studies to induce genes coding for typical cartilage matrix components and accelerate chondrogenic differentiation, making it the standard constituent in most cultivation media used for the assessment of chondrogenesis associated with various stem cell types on carrier matr...

journal_title：BMC biotechnology

authors： Huang Y,Seitz D,Chevalier Y,Müller PE,Jansson V,Klar RM

更新日期：2020-08-27 00:00:00

abstract：BACKGROUND:Social amoeba, Dictyostelium discoideum, is a well-established model organism for studying cellular physiology and developmental pattern formation. Its haploid genome facilitates functional analysis of genes by a single round of mutagenesis including targeted disruption. Although the efficient generation of ...

journal_title：BMC biotechnology

authors： Mukai A,Ichiraku A,Horikawa K

更新日期：2016-04-14 00:00:00

abstract：BACKGROUND:Taxol is an effective antineoplastic agent, originally extracted from the bark of Taxus brevifolia with a low yield. Many attempts have been made to produce Taxol by chemical synthesis, semi-synthesis and plant tissue cultures. However, to date, the availability of this compound is not sufficient to satisfy ...

journal_title：BMC biotechnology

authors： Bestoso F,Ottaggio L,Armirotti A,Balbi A,Damonte G,Degan P,Mazzei M,Cavalli F,Ledda B,Miele M

更新日期：2006-12-06 00:00:00

abstract：BACKGROUND:Anti-NMDA receptor encephalitis (ANRE) is a potentially lethal disease attributed to auto-antibodies against the N-methyl-D-aspartate receptor (NMDAR). Full recovery is possible if therapy is initiated early in the disease course. Detection of ANRE antibodies in the cerebrospinal fluid (CSF) is essential for...

journal_title：BMC biotechnology

authors： Sharma R,Al-Saleem FH,Puligedda RD,Rattelle A,Lynch DR,Dessain SK

更新日期：2018-06-27 00:00:00

abstract：BACKGROUND:DsRed the red fluorescent protein (RFP) isolated from Discosoma sp. coral holds much promise as a genetically and spectrally distinct alternative to green fluorescent protein (GFP) for application in mice. Widespread use of DsRed has been hampered by several issues resulting in the inability to establish and...

journal_title：BMC biotechnology

authors： Long JZ,Lackan CS,Hadjantonakis AK

更新日期：2005-07-04 00:00:00

abstract：BACKGROUND:We have investigated the possibility and feasibility of producing the HPV-11 L1 major capsid protein in transgenic Arabidopsis thaliana ecotype Columbia and Nicotiana tabacum cv. Xanthi as potential sources for an inexpensive subunit vaccine. RESULTS:Transformation of plants was only achieved with the HPV-1...

journal_title：BMC biotechnology

authors： Kohl TO,Hitzeroth II,Christensen ND,Rybicki EP

更新日期：2007-09-12 00:00:00

abstract：BACKGROUND:Interleukin-17 (IL-17), the characteristic cytokine secreted by T helper 17 lymphocytes (Th17 cells), plays a pivotal role in host defense and many inflammatory or autoimmune diseases. The aim of this study was to obtain purified protein caprine IL-17A (cIL-17A) as an antigen for preparing an IL-17A-specific...

journal_title：BMC biotechnology

authors： Gao Y,Sang FF,Meng L,Wang Y,Ma WT,Chen K

更新日期：2019-07-17 00:00:00

abstract：BACKGROUND:There are significant differences in the culture conditions between small-scale screenings and large-scale fermentation processes. Production processes are usually conducted in fed-batch cultivation mode with active pH-monitoring and control. In contrast, screening experiments in shake flasks are usually con...

journal_title：BMC biotechnology

authors： Scheidle M,Dittrich B,Klinger J,Ikeda H,Klee D,Büchs J

更新日期：2011-03-23 00:00:00