Characterization of mutations and loss of heterozygosity of p53 and K-ras2 in pancreatic cancer cell lines by immobilized polymerase chain reaction.

abstract：BACKGROUND:Quantum dots (QDs) have been considered as a new and efficient probe for labeling cells non-invasively in vitro and in vivo, but fairly little is known about how QDs are eliminated from cells after labeling. The purpose of this study is to investigate the metabolism of QDs in different type of cells. RESULT...

journal_title：BMC biotechnology

authors： Pi QM,Zhang WJ,Zhou GD,Liu W,Cao Y

更新日期：2010-05-06 00:00:00

abstract：BACKGROUND:Establishing highly productive clonal cell lines with constant productivity over 2-3 months of continuous culture remains a tedious task requiring the screening of tens of thousands of clonal colonies. In addition, long-term cultivation of many candidate lines derived in the absence of drug selection pressur...

journal_title：BMC biotechnology

authors： Orlova NA,Kovnir SV,Hodak JA,Vorobiev II,Gabibov AG,Skryabin KG

更新日期：2014-06-14 00:00:00

abstract：BACKGROUND:Protein cross-coupling reactions demand high yields, especially if the products are intended for bioanalytics, like enzyme-linked immunosorbent assays. Amongst other factors, the coupling yield depends on the concentration of the proteins being used for coupling. Protein supercharging of enzymes can increase...

journal_title：BMC biotechnology

authors： Prasse AA,Zauner T,Büttner K,Hoffmann R,Zuchner T

更新日期：2014-10-18 00:00:00

abstract：BACKGROUND:Polysialic acid (polySia) is a carbohydrate modification of the neural cell adhesion molecule (NCAM), which is implicated in neural differentiation and plays an important role in tumor development and metastasis. Polysialylation of NCAM is mediated by two Golgi-resident polysialyltransferases (polyST) ST8Sia...

journal_title：BMC biotechnology

authors： Somplatzki S,Mühlenhoff M,Kröger A,Gerardy-Schahn R,Böldicke T

更新日期：2017-05-12 00:00:00

abstract：BACKGROUND:The cattle ticks, Boophilus spp., affect cattle production in tropical and subtropical regions of the world. Tick vaccines constitute a cost-effective and environmentally friendly alternative to tick control. The recombinant B. microplus Bm86 protective antigen has been shown to protect cattle against tick i...

journal_title：BMC biotechnology

authors： Canales M,Almazán C,Naranjo V,Jongejan F,de la Fuente J

更新日期：2009-03-31 00:00:00

abstract：BACKGROUND:Fungal laccases are multicopper oxidases with huge applicability in different sectors. Here, we describe the development of a set of high-throughput colorimetric assays for screening laccase libraries in directed evolution studies. RESULTS:Firstly, we designed three colorimetric assays based on the oxidatio...

journal_title：BMC biotechnology

authors： Pardo I,Chanagá X,Vicente AI,Alcalde M,Camarero S

更新日期：2013-10-26 00:00:00

abstract：BACKGROUND:Dengue is a public health problem of global significance for which there is neither an effective antiviral therapy nor a preventive vaccine. It is a mosquito-borne viral disease, caused by dengue (DEN) viruses, which are members of the Flaviviridae family. There are four closely related serotypes, DEN-1, DEN...

journal_title：BMC biotechnology

authors： Khanam S,Rajendra P,Khanna N,Swaminathan S

更新日期：2007-02-15 00:00:00

abstract：BACKGROUND:Natural rubber produced by plants, known as polyisoprene, is the most widely used isoprenoid polymer. Plant polyisoprenes can be classified into two types; cis-polyisoprene and trans-polyisoprene, depending on the type of polymerization of the isoprene unit. More than 2000 species of higher plants produce la...

journal_title：BMC biotechnology

authors： Chen R,Harada Y,Bamba T,Nakazawa Y,Gyokusen K

更新日期：2012-10-30 00:00:00

abstract：BACKGROUND:Taxol is an effective antineoplastic agent, originally extracted from the bark of Taxus brevifolia with a low yield. Many attempts have been made to produce Taxol by chemical synthesis, semi-synthesis and plant tissue cultures. However, to date, the availability of this compound is not sufficient to satisfy ...

journal_title：BMC biotechnology

authors： Bestoso F,Ottaggio L,Armirotti A,Balbi A,Damonte G,Degan P,Mazzei M,Cavalli F,Ledda B,Miele M

更新日期：2006-12-06 00:00:00

abstract：BACKGROUND:In the previous study, the cellulolytic Escherichia coli ZH-4 isolated from bovine rumen was found to show extracellular cellulase activity and could degrade cellulose in the culture. The goal of this work was to identify and characterize the secreted cellulase of E. coli ZH-4. It will be helpful to re-under...

journal_title：BMC biotechnology

authors： Pang J,Wang J,Liu Z,Zhang Q,Qi Q

更新日期：2019-08-27 00:00:00

abstract：BACKGROUND:Analysis of protein-protein interactions (PPIs) is a valuable approach for the characterization of huge networks of protein complexes or proteins of unknown function. Co-immunoprecipitation (coIP) using affinity resins coupled to protein A/G is the most widely used method for PPI detection. However, this tra...

journal_title：BMC biotechnology

authors： Liu Q,Chen Q,Wang J,Zhang Y,Zhou Y,Lin C,He W,He F,Xu D

更新日期：2010-10-28 00:00:00

abstract：BACKGROUND:New high-throughput sequencing technologies promise a very sensitive and high-resolution analysis of DNA methylation patterns in quantitative terms. However, a detailed and comprehensive comparison with existing validated DNA methylation analysis methods is not yet available. Therefore, a systematic cross-va...

journal_title：BMC biotechnology

authors： Potapova A,Albat C,Hasemeier B,Haeussler K,Lamprecht S,Suerbaum S,Kreipe H,Lehmann U

更新日期：2011-01-14 00:00:00

abstract：BACKGROUND:Protein glycosylation is of fundamental importance in many biological systems. The discovery of N-glycosylation in bacteria and the functional expression of the N-oligosaccharyltransferase PglB of Campylobacter jejuni in Escherichia coli enabled the production of engineered glycoproteins and the study of the...

journal_title：BMC biotechnology

authors： Ihssen J,Kowarik M,Wiesli L,Reiss R,Wacker M,Thöny-Meyer L

更新日期：2012-09-24 00:00:00

abstract：BACKGROUND:The green fluorescent protein (GFP) has been regarded as a valuable tool and widely applied as a biomarker in medical applications and diagnostics. A cost-efficient upstream expression system and an inexpensive downstream purification process will meet the demands of the GFP protein with high-purity. RESULT...

journal_title：BMC biotechnology

authors： Dong J,Ding X,Wang S

更新日期：2019-12-09 00:00:00

abstract：BACKGROUND:The therapeutic use of α-amidated peptides (e.g. calcitonin, glucagon-like peptide) has increased dramatically, but there are major impediments to wider use of such peptides. Larger peptides are expensive to synthesize, and short plasma half-lives frequently limit the clinical circumstances in which the pept...

journal_title：BMC biotechnology

authors： Carlson KR,Pomerantz SC,Li J,Vafa O,Naso M,Strohl W,Mains RE,Eipper BA

更新日期：2015-06-27 00:00:00

abstract：BACKGROUND:Construction of high quality cDNA libraries from the usually low amounts of eukaryotic mRNA extracted from environmental samples is essential in functional metatranscriptomics for the selection of functional, full-length genes encoding proteins of interest. Many of the inserts in libraries constructed by sta...

journal_title：BMC biotechnology

authors： Yadav RK,Barbi F,Ziller A,Luis P,Marmeisse R,Reddy MS,Fraissinet-Tachet L

更新日期：2014-09-03 00:00:00

abstract：BACKGROUND:Eleven new human polyomaviruses (HPyVs) have been identified in the last decade. Serological studies show that these novel HPyVs sub-clinically infect humans at an early age. The routes of infection, entry pathways, and cell tropism of new HPyVs remain unknown. VP1 proteins of polyomaviruses can assembly int...

journal_title：BMC biotechnology

authors： Norkiene M,Stonyte J,Ziogiene D,Mazeike E,Sasnauskas K,Gedvilaite A

更新日期：2015-08-04 00:00:00

abstract：BACKGROUND:Expression vector engineering technology is one of the most convenient and timely method for cell line development to meet the rising demand of novel production cell line with high productivity. Destabilization of dihydrofolate reductase (dhfr) selection marker by addition of AU-rich elements and murine orni...

journal_title：BMC biotechnology

authors： Chin CL,Chin HK,Chin CS,Lai ET,Ng SK

更新日期：2015-06-02 00:00:00

abstract：BACKGROUND:CRISPR/Cas9 is widely used for precise genetic editing in various organisms. CRISPR/Cas9 editing may in many plants be hampered by the presence of complex and high ploidy genomes and inefficient or poorly controlled delivery of the CRISPR/Cas9 components to gamete cells or cells with regenerative potential. ...

journal_title：BMC biotechnology

authors： Petersen BL,Möller SR,Mravec J,Jørgensen B,Christensen M,Liu Y,Wandall HH,Bennett EP,Yang Z

更新日期：2019-06-17 00:00:00

abstract：BACKGROUND:von Willebrand factor (VWF) is a key load bearing domain for mamalian cell adhesion by binding various macromolecular ligands in extracellular matrix such as, collagens, elastin, and glycosaminoglycans. Interestingly, vWF like domains are also commonly found in load bearing systems of marine organisms such a...

journal_title：BMC biotechnology

authors： Yoo HY,Song YH,Foo M,Seo E,Hwang DS,Seo JH

更新日期：2016-02-16 00:00:00

abstract：BACKGROUND:Ultrasound and microbubbles (USMB) have been shown to enhance the intracellular uptake of molecules, generally thought to occur as a result of sonoporation. The underlying mechanism associated with USMB-enhanced intracellular uptake such as membrane disruption and endocytosis may also be associated with USMB...

journal_title：BMC biotechnology

authors： Hussein F,Antonescu C,Karshafian R

更新日期：2017-05-18 00:00:00

abstract：BACKGROUND:Interest in using plants for production of recombinant proteins such as monoclonal antibodies is growing, but proteolytic degradation, leading to a loss of functionality and complications in downstream purification, is still a serious problem. RESULTS:In this study, we investigated the dynamics of the assem...

journal_title：BMC biotechnology

authors： Hehle VK,Paul MJ,Drake PM,Ma JK,van Dolleweerd CJ

更新日期：2011-12-30 00:00:00

abstract：BACKGROUND:Polymerase chain reaction (PCR) is one of the most important developments in modern biotechnology. However, PCR is known to introduce biases, especially during multiplex reactions. Recent studies have implicated the DNA polymerase as the primary source of bias, particularly initiation of polymerization on th...

journal_title：BMC biotechnology

authors： Pan W,Byrne-Steele M,Wang C,Lu S,Clemmons S,Zahorchak RJ,Han J

更新日期：2014-01-30 00:00:00

abstract：BACKGROUND:The ubiquitin protein is present in all eukaryotic cells and promoters from ubiquitin genes are good candidates to regulate the constitutive expression of transgenes in plants. Therefore, two switchgrass (Panicum virgatum L.) ubiquitin genes (PvUbi1 and PvUbi2) were cloned and characterized. Reporter constru...

journal_title：BMC biotechnology

authors： Mann DG,King ZR,Liu W,Joyce BL,Percifield RJ,Hawkins JS,LaFayette PR,Artelt BJ,Burris JN,Mazarei M,Bennetzen JL,Parrott WA,Stewart CN Jr

更新日期：2011-07-11 00:00:00

abstract：BACKGROUND:Nanoparticles appear to be promising devices for application in the agriculture and food industries, but information regarding the response of plants to contact with nano-devices is scarce. Toxic effects may be imposed depending on the type and concentration of nanoparticle as well as time of exposure. A num...

journal_title：BMC biotechnology

authors： Santos AR,Miguel AS,Macovei A,Maycock C,Balestrazzi A,Oliva A,Fevereiro P

更新日期：2013-12-20 00:00:00

abstract：BACKGROUND:Clustered Regularly Interspaced Short Palindromic Repeats/CRISPR associated protein 9 (CRISPR/Cas9) has been wildly used to generate gene knockout models through inducing indels causing frame-shift. However, there are few studies concerning the post-transcript effects caused by CRISPR-mediated genome editing...

journal_title：BMC biotechnology

authors： Tang JX,Chen D,Deng SL,Li J,Li Y,Fu Z,Wang XX,Zhang Y,Chen SR,Liu YX

更新日期：2018-10-03 00:00:00

abstract：BACKGROUND:There is an imperative necessity for alternative sources of energy able to reduce the world dependence of fossil oil. One of the most successful options is ethanol obtained mainly from sugarcane and corn fermentation. The foremost residue from sugarcane industry is the bagasse, a rich lignocellulosic raw mat...

journal_title：BMC biotechnology

authors： Robl D,Delabona Pda S,Mergel CM,Rojas JD,Costa Pdos S,Pimentel IC,Vicente VA,da Cruz Pradella JG,Padilla G

更新日期：2013-10-31 00:00:00

abstract：BACKGROUND:Oleaginous yeast species are an alternative for the production of lipids or triacylglycerides (TAGs). These yeasts are usually non-pathogenic and able to store TAGs ranging from 20 % to 70 % of their cell mass depending on culture conditions. TAGs originating from oleaginous yeasts can be used as the so-call...

journal_title：BMC biotechnology

authors： Lamers D,van Biezen N,Martens D,Peters L,van de Zilver E,Jacobs-van Dreumel N,Wijffels RH,Lokman C

更新日期：2016-05-27 00:00:00

abstract：BACKGROUND:Cell-penetrating peptides (CPPs) have been widely used as carriers to transport different molecules into living cells, whereas messenger RNAs (mRNAs) have been utilized as target molecules for the prevention and treatment of various diseases. However, the instability of CPPs and mRNAs has limited their appli...

journal_title：BMC biotechnology

authors： Sun Y,Sun Y,Zhao R,Gao K

更新日期：2016-05-28 00:00:00

abstract：BACKGROUND:One of the most commonly used vectors for gene therapy is the adenoviral vector; its ability to tightly regulate transgene expression is critical for optimizing therapeutic outcomes. The tetracycline-regulated system (especially the Tet-On system) for gene expression is one of the most valuable tools for con...

journal_title：BMC biotechnology

authors： Chen H,Wang D,Xia R,Mao Q,Xia H

更新日期：2015-02-13 00:00:00