Abstract:
BACKGROUND:The presence of coliform bacteria is routinely assessed to establish the microbiological safety of water supplies and raw or processed foods. Coliforms are a group of lactose-fermenting Enterobacteriaceae, which most likely acquired the lacZ gene by horizontal transfer and therefore constitute a polyphyletic group. Among this group of bacteria is Escherichia coli, the pathogen that is most frequently associated with foodborne disease outbreaks and is often identified by β-glucuronidase enzymatic activity or by the redundant detection of uidA by PCR. Because a significant fraction of essential E. coli genes are preserved throughout the bacterial kingdom, alternative oligonucleotide primers for specific E. coli detection are not easily identified. RESULTS:In this manuscript, two strategies were used to design oligonucleotide primers with differing levels of specificity for the simultaneous detection of total coliforms and E. coli by multiplex PCR. A consensus sequence of lacZ and the orphan gene yaiO were chosen as targets for amplification, yielding 234 bp and 115 bp PCR products, respectively. CONCLUSIONS:The assay designed in this work demonstrated superior detection ability when tested with lab collection and dairy isolated lactose-fermenting strains. While lacZ amplicons were found in a wide range of coliforms, yaiO amplification was highly specific for E. coli. Additionally, yaiO detection is non-redundant with enzymatic methods.
journal_name
BMC Biotechnoljournal_title
BMC biotechnologyauthors
Molina F,López-Acedo E,Tabla R,Roa I,Gómez A,Rebollo JEdoi
10.1186/s12896-015-0168-2subject
Has Abstractpub_date
2015-06-04 00:00:00pages
48issn
1472-6750pii
10.1186/s12896-015-0168-2journal_volume
15pub_type
杂志文章abstract:BACKGROUND:Genetically encoded sensors developed on the basis of green fluorescent protein (GFP)-like proteins are becoming more and more popular instruments for monitoring cellular analytes and enzyme activities in living cells and transgenic organisms. In particular, a number of Ca2+ sensors have been developed, eith...
journal_title:BMC biotechnology
pub_type: 杂志文章
doi:10.1186/1472-6750-7-37
更新日期:2007-06-29 00:00:00
abstract:BACKGROUND:As with many plant species, current genome editing strategies in soybean are initiated by stably transforming a gene that encodes an engineered nuclease into the genome. Expression of the transgene results in a double-stranded break and repair at the targeted locus, oftentimes resulting in mutation(s) at the...
journal_title:BMC biotechnology
pub_type: 杂志文章
doi:10.1186/s12896-020-00604-3
更新日期:2020-02-24 00:00:00
abstract:BACKGROUND:In recent years, cold-active esterases have received increased attention due to their attractive properties for some industrial applications such as high catalytic activity at low temperatures. RESULTS:An esterase-encoding gene (estS, 909 bp) from Serratia sp. was identified, cloned and expressed in Escheri...
journal_title:BMC biotechnology
pub_type: 杂志文章
doi:10.1186/s12896-016-0235-3
更新日期:2016-01-22 00:00:00
abstract:BACKGROUND:The ketoisovalerate reductase (EC 1.2.7.7 ) is required for the formation of beauvericin via the nonribosomal peptide synthetase biosynthetic pathway. It catalyzes the NADPH-specific reduction of ketoisovaleric acid to hydroxyisovalerate. However, little is known about the bioinformatics' data about the 2-Ki...
journal_title:BMC biotechnology
pub_type: 杂志文章
doi:10.1186/1472-6750-12-55
更新日期:2012-08-23 00:00:00
abstract:BACKGROUND:Cymbidium faberi is one of the oldest cultivars of oriental orchids, with an elegant flower fragrance. In order to investigate the molecular mechanism and the functions of related proteins in the methyl jasmonate (MeJA) signaling pathway, one of the main components of flower fragrance in C. faberi, yeast one...
journal_title:BMC biotechnology
pub_type: 杂志文章
doi:10.1186/s12896-020-0599-2
更新日期:2020-01-16 00:00:00
abstract:BACKGROUND:Analogues of vitamin D3 are extensively used in the treatment of various illnesses, such as osteoporosis, inflammatory skin diseases, and cancer. Functional testing of new vitamin D3 analogues and formulations for improved systemic and topical administration is supported by sensitive screening methods that a...
journal_title:BMC biotechnology
pub_type: 杂志文章
doi:10.1186/1472-6750-11-33
更新日期:2011-04-07 00:00:00
abstract:BACKGROUND:Quantum dots (QDs) have been considered as a new and efficient probe for labeling cells non-invasively in vitro and in vivo, but fairly little is known about how QDs are eliminated from cells after labeling. The purpose of this study is to investigate the metabolism of QDs in different type of cells. RESULT...
journal_title:BMC biotechnology
pub_type: 杂志文章
doi:10.1186/1472-6750-10-36
更新日期:2010-05-06 00:00:00
abstract:BACKGROUND:This study aims to assess suitability of hydroponic technology for treatment of brewery wastewater in a hydroponic bioreactor using Typha latifolia. Triplicated hydroponic bioreactor treatment units were designed, constructed and operated at a hydraulic retention time of 5 days with different surface loading...
journal_title:BMC biotechnology
pub_type: 杂志文章
doi:10.1186/s12896-018-0484-4
更新日期:2018-11-22 00:00:00
abstract:BACKGROUND:The antiphospholipid syndrome (APS), characterised by recurrent miscarriage and thrombosis, is a significant cause of morbidity and mortality. Domain I (DI) of human beta 2 glycoprotein I (beta2GPI) is thought to contain crucial antibody binding epitopes for antiphospholipid antibodies (aPL), which are criti...
journal_title:BMC biotechnology
pub_type: 杂志文章
doi:10.1186/1472-6750-6-8
更新日期:2006-02-10 00:00:00
abstract:BACKGROUND:Construction of high quality cDNA libraries from the usually low amounts of eukaryotic mRNA extracted from environmental samples is essential in functional metatranscriptomics for the selection of functional, full-length genes encoding proteins of interest. Many of the inserts in libraries constructed by sta...
journal_title:BMC biotechnology
pub_type: 杂志文章
doi:10.1186/1472-6750-14-80
更新日期:2014-09-03 00:00:00
abstract:BACKGROUND:The ability to produce the same recombinant protein in both prokaryotic and eukaryotic cells offers many experimental opportunities. However, the cloning of the same gene into multiple plasmids is required, which is time consuming, laborious and still may not produce soluble, stable protein in sufficient qua...
journal_title:BMC biotechnology
pub_type: 杂志文章
doi:10.1186/1472-6750-12-54
更新日期:2012-08-23 00:00:00
abstract:BACKGROUND:In a previous study, anthocyanin levels in potato plants were increased by manipulating genes connected with the flavonoid biosynthesis pathway. However, starch content and tuber yield were dramatically reduced in the transgenic plants, which over-expressed dihydroflavonol reductase (DFR). RESULTS:Transgeni...
journal_title:BMC biotechnology
pub_type: 杂志文章
doi:10.1186/1472-6750-8-25
更新日期:2008-03-05 00:00:00
abstract:BACKGROUND:Acetyl-xylan esterase (AXE, EC 3.1.1.72) hydrolyses acetate group from the linear chain of xylopyranose residues bound by β-1,4-linkage. The enzyme finds commercial applications in bio-bleaching of wood pulp, treating animal feed to increase digestibility, processing food to increase clarification and conver...
journal_title:BMC biotechnology
pub_type: 杂志文章
doi:10.1186/1472-6750-11-52
更新日期:2011-05-16 00:00:00
abstract:BACKGROUND:Urease B is an important virulence factor that is required for Helicobacter pylori to colonise the gastric mucosa. Mouse monoclonal antibodies (mAbs) that inhibit urease B enzymatic activity will be useful as vaccines for the prevention and treatment of H. pylori infection. Here, we produced murine mAbs agai...
journal_title:BMC biotechnology
pub_type: 杂志文章
doi:10.1186/1472-6750-10-84
更新日期:2010-11-30 00:00:00
abstract:BACKGROUND:The routine generation of transgenic plants involves analysis of transgene integration into the host genome by means of Southern blotting. However, this technique cannot distinguish between uniformly transformed tissues and the presence of a mixture of transgenic and non-transgenic cells in the same tissue. ...
journal_title:BMC biotechnology
pub_type: 杂志文章
doi:10.1186/1472-6750-10-53
更新日期:2010-07-16 00:00:00
abstract:BACKGROUND:Cost-effective production of industrially important enzymes is a key for their successful exploitation on industrial scale. Keeping in view the extensive industrial applications of lignin peroxidase (LiP), this study was performed to purify and characterize the LiP from an indigenous strain of Trametes versi...
journal_title:BMC biotechnology
pub_type: 杂志文章
doi:10.1186/1472-6750-12-46
更新日期:2012-08-03 00:00:00
abstract:BACKGROUND:Polymerase chain reaction (PCR) is one of the most important developments in modern biotechnology. However, PCR is known to introduce biases, especially during multiplex reactions. Recent studies have implicated the DNA polymerase as the primary source of bias, particularly initiation of polymerization on th...
journal_title:BMC biotechnology
pub_type: 杂志文章
doi:10.1186/1472-6750-14-10
更新日期:2014-01-30 00:00:00
abstract:BACKGROUND:microRNAs (miRNAs) are short non-coding RNAs that fine-tune gene expression. The aberrant expression of miRNAs is associated with many diseases and they have both therapeutic and biomarker potential. However, our understanding of their usefulness is dependent on the tools we have to study them. Previous stud...
journal_title:BMC biotechnology
pub_type: 杂志文章
doi:10.1186/s12896-018-0421-6
更新日期:2018-03-16 00:00:00
abstract:BACKGROUND:Plasminogen activator inhibitor type-2 (PAI-2, SERPINB2) is an irreversible, specific inhibitor of the urokinase plasminogen activator (uPA). Since overexpression of uPA at the surface of cancer cells is linked to malignancy, targeting of uPA by exogenous recombinant PAI-2 has been proposed as the basis of p...
journal_title:BMC biotechnology
pub_type: 杂志文章
doi:10.1186/1472-6750-9-43
更新日期:2009-05-14 00:00:00
abstract:BACKGROUND:Heat-stable antifungal factor (HSAF) is a newly identified broad-spectrum antifungal antibiotic from the biocontrol agent Lysobacter enzymogenes and is regarded as a potential biological pesticide, due to its novel mode of action. However, the production level of HSAF is quite low, and little research has re...
journal_title:BMC biotechnology
pub_type: 杂志文章
doi:10.1186/s12896-018-0478-2
更新日期:2018-10-24 00:00:00
abstract:BACKGROUND:The recent H1N1 influenza pandemic illustrated the shortcomings of the vaccine manufacturing process. The A/California/07/2009 H1N1 pandemic influenza vaccine or A(H1N1)pdm09 was available late and in short supply as a result of delays in production caused by low yields and poor antigen stability. Recombinan...
journal_title:BMC biotechnology
pub_type: 杂志文章
doi:10.1186/1472-6750-12-77
更新日期:2012-10-30 00:00:00
abstract:BACKGROUND:Xylanase can replace chemical additives to improve the volume and sensory properties of bread in the baking. Suitable baking xylanase with improved yield will promote the application of xylanase in baking industry. The xylanase XYNZG from the Plectosphaerella cucumerina has been previously characterized by h...
journal_title:BMC biotechnology
pub_type: 杂志文章
doi:10.1186/s12896-014-0107-7
更新日期:2014-12-16 00:00:00
abstract:BACKGROUND:The isothermal amplification of RNA in vitro has been used for the study of in vitro evolution of RNA. Although Qβ replicase has been traditionally used as an enzyme for this purpose, we planned to use norovirus replicase (NV3D(pol)) due to its structural simplicity in the scope of in vitro autonomous evolut...
journal_title:BMC biotechnology
pub_type: 杂志文章
doi:10.1186/1472-6750-13-85
更新日期:2013-10-09 00:00:00
abstract:BACKGROUND:The insect cell line is a critical component in the production of recombinant proteins in the baculovirus expression system and new cell lines hold the promise of increasing both quantity and quality of protein production. RESULTS:Seventy cell lines were established by single-cell cloning from a primary cul...
journal_title:BMC biotechnology
pub_type: 杂志文章
doi:10.1186/1472-6750-10-50
更新日期:2010-07-05 00:00:00
abstract:BACKGROUND:Dengue is a public health problem of global significance for which there is neither an effective antiviral therapy nor a preventive vaccine. It is a mosquito-borne viral disease, caused by dengue (DEN) viruses, which are members of the Flaviviridae family. There are four closely related serotypes, DEN-1, DEN...
journal_title:BMC biotechnology
pub_type: 杂志文章
doi:10.1186/1472-6750-7-10
更新日期:2007-02-15 00:00:00
abstract:BACKGROUND:In spite of a great number of monomeric fluorescent proteins developed in the recent years, the reported fluorescent protein-based FRET pairs are still characterized by a number of disadvantageous features, complicating their use as reporters in cell biology and for high-throughput cell-based screenings. RE...
journal_title:BMC biotechnology
pub_type: 杂志文章
doi:10.1186/1472-6750-9-24
更新日期:2009-03-25 00:00:00
abstract:BACKGROUND:The ability of cytosine deaminase (CD) to convert the antifungal agent 5-fluorocytosine (5-FC) into one of the most potent and largely used anticancer compound such as 5-fluorouracil (5-FU) raised considerable interest in this enzyme to model gene or antibody - directed enzyme-prodrug therapy (GDEPT/ADEPT) a...
journal_title:BMC biotechnology
pub_type: 杂志文章
doi:10.1186/1472-6750-8-68
更新日期:2008-09-10 00:00:00
abstract:BACKGROUND:Advances in optical imaging modalities and the continued evolution of genetically-encoded fluorescent proteins are coming together to facilitate the study of cell behavior at high resolution in living organisms. As a result, imaging using autofluorescent protein reporters is gaining popularity in mouse trans...
journal_title:BMC biotechnology
pub_type: 杂志文章
doi:10.1186/1472-6750-4-33
更新日期:2004-12-24 00:00:00
abstract:BACKGROUND:Clostridial co-culture containing cellulolytic and solventogenic species is a potential consolidated bioprocessing (CBP) approach for producing biochemicals and biofuels from cellulosic biomass. It has been demonstrated that the rate of cellulose utilization in the co-culture of Clostridium acetobutylicum an...
journal_title:BMC biotechnology
pub_type: 杂志文章
doi:10.1186/1472-6750-13-95
更新日期:2013-11-04 00:00:00
abstract:BACKGROUND:Porcine reproductive and respiratory syndrome virus (PRRSV) causes major economic losses in the pig industry worldwide. In vivo, the virus infects a subpopulation of tissue macrophages. In vitro, PRRSV only replicates in primary pig macrophages and African green monkey kidney derived cells, such as Marc-145....
journal_title:BMC biotechnology
pub_type: 杂志文章
doi:10.1186/1472-6750-10-48
更新日期:2010-06-29 00:00:00