Generation and characterization of a human single-chain fragment variable (scFv) antibody against cytosine deaminase from yeast.

abstract：BACKGROUND:Whole genome amplification (WGA) promises to eliminate practical molecular genetic analysis limitations associated with genomic DNA (gDNA) quantity. We evaluated the performance of multiple displacement amplification (MDA) WGA using gDNA extracted from lymphoblastoid cell lines (N = 27) with a range of start...

journal_title：BMC biotechnology

authors： Bergen AW,Qi Y,Haque KA,Welch RA,Chanock SJ

更新日期：2005-09-16 00:00:00

abstract：BACKGROUND:Analogues of vitamin D3 are extensively used in the treatment of various illnesses, such as osteoporosis, inflammatory skin diseases, and cancer. Functional testing of new vitamin D3 analogues and formulations for improved systemic and topical administration is supported by sensitive screening methods that a...

journal_title：BMC biotechnology

authors： Staunstrup NH,Sharma N,Bak RO,Svensson L,Petersen TK,Aarenstrup L,Kristiansen K,Bolund L,Mikkelsen JG

更新日期：2011-04-07 00:00:00

abstract：BACKGROUND:Clostridial co-culture containing cellulolytic and solventogenic species is a potential consolidated bioprocessing (CBP) approach for producing biochemicals and biofuels from cellulosic biomass. It has been demonstrated that the rate of cellulose utilization in the co-culture of Clostridium acetobutylicum an...

journal_title：BMC biotechnology

authors： Salimi F,Mahadevan R

更新日期：2013-11-04 00:00:00

abstract：BACKGROUND:The identification of known mutations in a cell population is important for clinical applications and basic cancer research. In this work an immobilized form of the polymerase chain reaction, referred to as polony technology, was used to detect mutations as well as gene deletions, resulting in loss of hetero...

journal_title：BMC biotechnology

authors： Butz J,Wickstrom E,Edwards J

更新日期：2003-07-23 00:00:00

abstract：BACKGROUND:Tetanus neurotoxin (TeNT) is taken up at nerve terminals and undergoes retrograde migration. The toxic properties of TeNT reside in the toxin light chain (L), but like complete TeNT, the TeNT heavy chain (TTH) and the C-terminal domain (TTC) alone can bind and enter into neurons. Here, we explored whether at...

journal_title：BMC biotechnology

authors： Watanabe Y,Matsuba T,Nakanishi M,Une M,Hanajima R,Nakashima K

更新日期：2018-06-11 00:00:00

abstract：BACKGROUND:Chinese hamster ovary (CHO) cells have become the host of choice for the production of recombinant proteins, due to their capacity for correct protein folding, assembly, and posttranslational modifications. The most widely used system for recombinant proteins is the gene amplification procedure that uses the...

journal_title：BMC biotechnology

authors： Jamnikar U,Nikolic P,Belic A,Blas M,Gaser D,Francky A,Laux H,Blejec A,Baebler S,Gruden K

更新日期：2015-10-23 00:00:00

abstract：BACKGROUND:Mesenchymal stem cells (MSCs) are of great interest in bone regenerative medicine due to their osteogenic potential and trophic effects. However, challenges to large-scale production of MSCs can hinder the translation of MSC therapies. 3D Microcarrier (MC)-based MSC culture presents a scalable and cost-effec...

journal_title：BMC biotechnology

authors： Shekaran A,Sim E,Tan KY,Chan JK,Choolani M,Reuveny S,Oh S

更新日期：2015-10-31 00:00:00

abstract：BACKGROUND:In the previous study, the cellulolytic Escherichia coli ZH-4 isolated from bovine rumen was found to show extracellular cellulase activity and could degrade cellulose in the culture. The goal of this work was to identify and characterize the secreted cellulase of E. coli ZH-4. It will be helpful to re-under...

journal_title：BMC biotechnology

authors： Pang J,Wang J,Liu Z,Zhang Q,Qi Q

更新日期：2019-08-27 00:00:00

abstract：BACKGROUND:Each year, influenza is responsible for hundreds of thousand cases of illness and deaths worldwide. Due to the virus' fast mutation rate, the World Health Organization (WHO) is constantly on alert to rapidly respond to emerging pandemic strains. Although anti-viral therapies exist, the most proficient way to...

journal_title：BMC biotechnology

authors： Thompson CM,Petiot E,Mullick A,Aucoin MG,Henry O,Kamen AA

更新日期：2015-05-16 00:00:00

abstract：BACKGROUND:L-ornithine is effective in the treatment of liver diseases and helps strengthen the heart. The commercial applications mean that efficient biotechnological production of L-ornithine has become increasingly necessary. Adaptive evolution strategies have been proven a feasible and efficient technique to achiev...

journal_title：BMC biotechnology

authors： Jiang LY,Chen SG,Zhang YY,Liu JZ

更新日期：2013-06-01 00:00:00

abstract：BACKGROUND:MicroRNAs are important regulators of gene expression at the post-transcriptional level and play an important role in many biological processes. Due to the important biological role it is of great interest to quantitatively determine their expression level in different biological settings. RESULTS:We descri...

journal_title：BMC biotechnology

authors： Balcells I,Cirera S,Busk PK

更新日期：2011-06-25 00:00:00

abstract：BACKGROUND:Recombinant factor VIII (FVIII), used for haemophilia A therapy, is one of the most challenging among the therapeutic proteins produced in heterologous expression systems. Deletion variant of FVIII, in which the entire domain B is replaced by a short linker peptide, was approved for medical use. Efficacy and...

journal_title：BMC biotechnology

authors： Orlova NA,Kovnir SV,Gabibov AG,Vorobiev II

更新日期：2017-03-24 00:00:00

abstract：BACKGROUND:Analysis of protein-protein interactions (PPIs) is a valuable approach for the characterization of huge networks of protein complexes or proteins of unknown function. Co-immunoprecipitation (coIP) using affinity resins coupled to protein A/G is the most widely used method for PPI detection. However, this tra...

journal_title：BMC biotechnology

authors： Liu Q,Chen Q,Wang J,Zhang Y,Zhou Y,Lin C,He W,He F,Xu D

更新日期：2010-10-28 00:00:00

abstract：BACKGROUND:Serine hydroxymethyltransferase (SHMT) is the key enzyme in L-serine enzymatic production, suggesting the importance of obtaining a SHMT with high activity. RESULTS:Here, a novel SHMT gene, glyA, was obtained through degenerate oligonucleotide-primed PCR and encoded a novel SHMT with 54.3% similarity to the...

journal_title：BMC biotechnology

authors： Jiang W,Chen L,Hu N,Yuan S,Li B,Liu Z

更新日期：2014-11-14 00:00:00

abstract：BACKGROUND:Social amoeba, Dictyostelium discoideum, is a well-established model organism for studying cellular physiology and developmental pattern formation. Its haploid genome facilitates functional analysis of genes by a single round of mutagenesis including targeted disruption. Although the efficient generation of ...

journal_title：BMC biotechnology

authors： Mukai A,Ichiraku A,Horikawa K

更新日期：2016-04-14 00:00:00

abstract：BACKGROUND:Plants may represent excellent alternatives to classical heterologous protein expression systems, especially for the production of biopharmaceuticals and vaccine components. Modern vaccines are becoming increasingly complex, with the incorporation of multiple antigens. Approaches towards developing an HIV va...

journal_title：BMC biotechnology

authors： Marusic C,Nuttall J,Buriani G,Lico C,Lombardi R,Baschieri S,Benvenuto E,Frigerio L

更新日期：2007-02-26 00:00:00

abstract：BACKGROUND:In recent years, cold-active esterases have received increased attention due to their attractive properties for some industrial applications such as high catalytic activity at low temperatures. RESULTS:An esterase-encoding gene (estS, 909 bp) from Serratia sp. was identified, cloned and expressed in Escheri...

journal_title：BMC biotechnology

authors： Jiang H,Zhang S,Gao H,Hu N

更新日期：2016-01-22 00:00:00

abstract：BACKGROUND:Despite positive reports on the efficacy of stem cell therapy for the treatment of cardiovascular disease, the nature of stem cell homing to ischemic tissues remains elusive. RESULTS:We used a mouse model of peripheral tissue ischemia to study the survival and homing capacity of dual reporter gene (eGFP/Luc...

journal_title：BMC biotechnology

authors： Everaert BR,Bergwerf I,De Vocht N,Ponsaerts P,Van Der Linden A,Timmermans JP,Vrints CJ

更新日期：2012-12-03 00:00:00

abstract：BACKGROUND:A method for inhibiting the expression of particular genes using external guide sequences (EGSs) has been developed in bacteria, mammalian cells and maize cells. RESULTS:To examine whether EGS technology can be used to down-regulate gene expression in Caenorhabditis elegans (C. elegans), we generated EGS-Ng...

journal_title：BMC biotechnology

authors： Yan Q,Zhao R,Zheng W,Yin C,Zhang B,Ma W

更新日期：2009-05-20 00:00:00

abstract：BACKGROUND:Nitrilases attract increasing attention due to their utility in the mild hydrolysis of nitriles. According to activity and gene screening, filamentous fungi are a rich source of nitrilases distinct in evolution from their widely examined bacterial counterparts. However, fungal nitrilases have been less explo...

journal_title：BMC biotechnology

authors： Kaplan O,Bezouška K,Plíhal O,Ettrich R,Kulik N,Vaněk O,Kavan D,Benada O,Malandra A,Sveda O,Veselá AB,Rinágelová A,Slámová K,Cantarella M,Felsberg J,Dušková J,Dohnálek J,Kotik M,Křen V,Martínková L

更新日期：2011-01-06 00:00:00

abstract：BACKGROUND:Interest in using plants for production of recombinant proteins such as monoclonal antibodies is growing, but proteolytic degradation, leading to a loss of functionality and complications in downstream purification, is still a serious problem. RESULTS:In this study, we investigated the dynamics of the assem...

journal_title：BMC biotechnology

authors： Hehle VK,Paul MJ,Drake PM,Ma JK,van Dolleweerd CJ

更新日期：2011-12-30 00:00:00

abstract：BACKGROUND:A modification of a standard method of fluorescence in situ hybridisation (FISH) is described, by which a combination of several substrates and probes on single microscope slides enables more accurate comparisons of the distribution and abundance of chromosomal sequences and improves the relatively low throu...

journal_title：BMC biotechnology

authors： Hasterok R,Dulawa J,Jenkins G,Leggett M,Langdon T

更新日期：2006-03-20 00:00:00

abstract：BACKGROUND:Human TGF-β3 has been used in many studies to induce genes coding for typical cartilage matrix components and accelerate chondrogenic differentiation, making it the standard constituent in most cultivation media used for the assessment of chondrogenesis associated with various stem cell types on carrier matr...

journal_title：BMC biotechnology

authors： Huang Y,Seitz D,Chevalier Y,Müller PE,Jansson V,Klar RM

更新日期：2020-08-27 00:00:00

abstract：BACKGROUND:Iron is an essential element in several pathways of microbial metabolism, and therefore low iron toxicity is expected on the usage of Fe nanoparticles (NPs). This study aims to determine the effect of Fe NPs on biosurfactant production by marine actinobacterium Nocardiopsis sp. MSA13A under solid state cultu...

journal_title：BMC biotechnology

authors： Kiran GS,Nishanth LA,Priyadharshini S,Anitha K,Selvin J

更新日期：2014-05-21 00:00:00

abstract：BACKGROUND:The presence of coliform bacteria is routinely assessed to establish the microbiological safety of water supplies and raw or processed foods. Coliforms are a group of lactose-fermenting Enterobacteriaceae, which most likely acquired the lacZ gene by horizontal transfer and therefore constitute a polyphyletic...

journal_title：BMC biotechnology

authors： Molina F,López-Acedo E,Tabla R,Roa I,Gómez A,Rebollo JE

更新日期：2015-06-04 00:00:00

abstract：BACKGROUND:Smoking and tobacco use continue to be the largest preventable causes of death globally. A novel therapeutic approach has recently been proposed: administration of an enzyme that degrades nicotine, the main addictive component of tobacco, minimizing brain exposure and reducing its reinforcing effects. Pre-cl...

journal_title：BMC biotechnology

authors： Thisted T,Biesova Z,Walmacq C,Stone E,Rodnick-Smith M,Ahmed SS,Horrigan SK,Van Engelen B,Reed C,Kalnik MW

更新日期：2019-08-02 00:00:00

abstract：BACKGROUND:Clustered Regularly Interspaced Short Palindromic Repeats/CRISPR associated protein 9 (CRISPR/Cas9) has been wildly used to generate gene knockout models through inducing indels causing frame-shift. However, there are few studies concerning the post-transcript effects caused by CRISPR-mediated genome editing...

journal_title：BMC biotechnology

authors： Tang JX,Chen D,Deng SL,Li J,Li Y,Fu Z,Wang XX,Zhang Y,Chen SR,Liu YX

更新日期：2018-10-03 00:00:00

abstract：BACKGROUND:In view of compliance with increasingly stringent environmental legislation, an eco-friendly treatment technology of industrial dyes and effluents is a major environmental challenge in the color industry. In present study, a promising and eco-friendly entrapment approach was adopted to immobilize purified ma...

journal_title：BMC biotechnology

authors： Bilal M,Asgher M

更新日期：2015-12-10 00:00:00

abstract：BACKGROUND:Microarray technology has been used extensively over the past 10 years for assessing gene expression, and has facilitated precise genetic profiling of everything from tumors to small molecule drugs. By contrast, arraying cell membranes in a manner which preserves their ability to mediate biochemical processe...

journal_title：BMC biotechnology

authors： Yamazaki V,Sirenko O,Schafer RJ,Nguyen L,Gutsmann T,Brade L,Groves JT

更新日期：2005-06-16 00:00:00

abstract：BACKGROUND:Splicing of DNA molecules is an important task in molecular biology that facilitates cloning, mutagenesis and creation of chimeric genes. Mutagenesis and DNA splicing techniques exist, some requiring restriction enzymes, and others utilize staggered reannealing approaches. RESULTS:A method for DNA splicing ...

journal_title：BMC biotechnology

authors： Ailenberg M,Goldenberg NM,Silverman M

更新日期：2005-09-01 00:00:00