Abstract:
BACKGROUND:A modification of a standard method of fluorescence in situ hybridisation (FISH) is described, by which a combination of several substrates and probes on single microscope slides enables more accurate comparisons of the distribution and abundance of chromosomal sequences and improves the relatively low throughput of standard FISH methods. RESULTS:The utility and application of multi-colour, multi-substrate FISH is illustrated by the simultaneous physical mapping of retrotransposon sequences to three species of Avena, and single locus BAC (bacterial artificial chromosome) clones and rDNA probes to three species of Brachypodium, demonstrating how this would enable better understanding of complex phylogenetic relationships among some of the species belonging to these two genera. CONCLUSION:The results show that use of multi-substrate chromosome preparations significantly increases the utility of FISH in comparative analyses of the distribution and abundance of chromosomal sequences in closely related plant species.
journal_name
BMC Biotechnoljournal_title
BMC biotechnologyauthors
Hasterok R,Dulawa J,Jenkins G,Leggett M,Langdon Tdoi
10.1186/1472-6750-6-20keywords:
subject
Has Abstractpub_date
2006-03-20 00:00:00pages
20issn
1472-6750pii
1472-6750-6-20journal_volume
6pub_type
杂志文章abstract:BACKGROUND:Asian rust (Phakopsora pachyrhizi) is a common disease in Brazilian soybean fields and it is difficult to control. To identify a biochemical candidate with potential to combat this disease, a new chitinase-like xylanase inhibitor protein (XIP) from coffee (Coffea arabica) (CaclXIP) leaves was cloned into the...
journal_title:BMC biotechnology
pub_type: 杂志文章
doi:10.1186/1472-6750-11-14
更新日期:2011-02-07 00:00:00
abstract:BACKGROUND:In view of compliance with increasingly stringent environmental legislation, an eco-friendly treatment technology of industrial dyes and effluents is a major environmental challenge in the color industry. In present study, a promising and eco-friendly entrapment approach was adopted to immobilize purified ma...
journal_title:BMC biotechnology
pub_type: 杂志文章
doi:10.1186/s12896-015-0227-8
更新日期:2015-12-10 00:00:00
abstract:BACKGROUND:The CRISPR/Cas9 genome editing system has greatly facilitated and expanded our capacity to engineer mammalian genomes, including targeted gene knock-outs. However, the phenotyping of the knock-out effect requires a high DNA editing efficiency. RESULTS:Here, we report a user-friendly strategy based on the ex...
journal_title:BMC biotechnology
pub_type: 杂志文章
doi:10.1186/s12896-016-0250-4
更新日期:2016-02-16 00:00:00
abstract:BACKGROUND:Eleven new human polyomaviruses (HPyVs) have been identified in the last decade. Serological studies show that these novel HPyVs sub-clinically infect humans at an early age. The routes of infection, entry pathways, and cell tropism of new HPyVs remain unknown. VP1 proteins of polyomaviruses can assembly int...
journal_title:BMC biotechnology
pub_type: 杂志文章
doi:10.1186/s12896-015-0187-z
更新日期:2015-08-04 00:00:00
abstract:BACKGROUND:This study aims to assess suitability of hydroponic technology for treatment of brewery wastewater in a hydroponic bioreactor using Typha latifolia. Triplicated hydroponic bioreactor treatment units were designed, constructed and operated at a hydraulic retention time of 5 days with different surface loading...
journal_title:BMC biotechnology
pub_type: 杂志文章
doi:10.1186/s12896-018-0484-4
更新日期:2018-11-22 00:00:00
abstract:BACKGROUND:Vascular endothelial growth factor (VEGF) is a key angiogenic factors. It plays an important role in both physiologic and pathologic angiogenesis and increases permeability across the vessels. Using antibody phage display technology, we obtained a novel anti-VEGFA IgG, named as FD006. In this study, the phar...
journal_title:BMC biotechnology
pub_type: 杂志文章
doi:10.1186/1472-6750-14-17
更新日期:2014-02-27 00:00:00
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journal_title:BMC biotechnology
pub_type: 杂志文章
doi:10.1186/s12896-015-0164-6
更新日期:2015-05-29 00:00:00
abstract:BACKGROUND:In the previous study, the cellulolytic Escherichia coli ZH-4 isolated from bovine rumen was found to show extracellular cellulase activity and could degrade cellulose in the culture. The goal of this work was to identify and characterize the secreted cellulase of E. coli ZH-4. It will be helpful to re-under...
journal_title:BMC biotechnology
pub_type: 杂志文章
doi:10.1186/s12896-019-0556-0
更新日期:2019-08-27 00:00:00
abstract:BACKGROUND:Despite positive reports on the efficacy of stem cell therapy for the treatment of cardiovascular disease, the nature of stem cell homing to ischemic tissues remains elusive. RESULTS:We used a mouse model of peripheral tissue ischemia to study the survival and homing capacity of dual reporter gene (eGFP/Luc...
journal_title:BMC biotechnology
pub_type: 杂志文章
doi:10.1186/1472-6750-12-93
更新日期:2012-12-03 00:00:00
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journal_title:BMC biotechnology
pub_type: 杂志文章
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更新日期:2008-09-10 00:00:00
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journal_title:BMC biotechnology
pub_type: 杂志文章
doi:10.1186/1472-6750-11-104
更新日期:2011-11-10 00:00:00
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journal_title:BMC biotechnology
pub_type: 杂志文章,收录出版
doi:10.1186/1472-6750-11-2
更新日期:2011-01-06 00:00:00
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journal_title:BMC biotechnology
pub_type: 杂志文章
doi:10.1186/1472-6750-12-16
更新日期:2012-05-03 00:00:00
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journal_title:BMC biotechnology
pub_type: 杂志文章
doi:10.1186/1472-6750-7-29
更新日期:2007-06-01 00:00:00
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journal_title:BMC biotechnology
pub_type: 杂志文章
doi:10.1186/s12896-017-0360-7
更新日期:2017-05-12 00:00:00
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journal_title:BMC biotechnology
pub_type: 杂志文章
doi:10.1186/1472-6750-9-24
更新日期:2009-03-25 00:00:00
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journal_title:BMC biotechnology
pub_type: 杂志文章
doi:10.1186/1472-6750-10-36
更新日期:2010-05-06 00:00:00
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journal_title:BMC biotechnology
pub_type: 杂志文章
doi:10.1186/s12896-018-0452-z
更新日期:2018-06-11 00:00:00
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journal_title:BMC biotechnology
pub_type: 杂志文章
doi:10.1186/1472-6750-7-52
更新日期:2007-08-27 00:00:00
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journal_title:BMC biotechnology
pub_type: 杂志文章
doi:10.1186/1472-6750-11-70
更新日期:2011-06-25 00:00:00
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journal_title:BMC biotechnology
pub_type: 杂志文章
doi:10.1186/1472-6750-6-14
更新日期:2006-03-06 00:00:00
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journal_title:BMC biotechnology
pub_type: 杂志文章
doi:10.1186/1472-6750-12-67
更新日期:2012-09-24 00:00:00
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journal_title:BMC biotechnology
pub_type: 杂志文章
doi:10.1186/1472-6750-11-21
更新日期:2011-03-15 00:00:00
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journal_title:BMC biotechnology
pub_type: 杂志文章
doi:10.1186/1472-6750-8-25
更新日期:2008-03-05 00:00:00
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journal_title:BMC biotechnology
pub_type: 杂志文章
doi:10.1186/1472-6750-5-7
更新日期:2005-02-11 00:00:00
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journal_title:BMC biotechnology
pub_type: 杂志文章
doi:10.1186/s12896-018-0488-0
更新日期:2018-12-14 00:00:00
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journal_title:BMC biotechnology
pub_type: 杂志文章
doi:10.1186/1472-6750-7-56
更新日期:2007-09-12 00:00:00
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journal_title:BMC biotechnology
pub_type: 杂志文章
doi:10.1186/s12896-016-0247-z
更新日期:2016-02-16 00:00:00
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journal_title:BMC biotechnology
pub_type: 杂志文章
doi:10.1186/s12896-019-0502-1
更新日期:2019-01-28 00:00:00
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journal_title:BMC biotechnology
pub_type: 杂志文章
doi:10.1186/s12896-019-0543-5
更新日期:2019-07-17 00:00:00