Abstract:
BACKGROUND:The proteomics literature has seen a proliferation of publications that seek to apply the rapidly improving technology of 2D gels to study various biological systems. However, there is a dearth of systematic studies that have investigated appropriate statistical approaches to analyse the data from these experiments. RESULTS:Comparison of the effects of statistical pre-processing on the results of two sample t-tests suggests that the results of 2D gel experiments and by extension the conclusions derived from these experiments are not independent of the statistical protocol used. CONCLUSIONS:This study suggests that there is a need for well-conducted validation studies to establish optimal statistical techniques to be used on such data sets.
journal_name
BMC Biotechnoljournal_title
BMC biotechnologyauthors
Meleth S,Deshane J,Kim Hdoi
10.1186/1472-6750-5-7keywords:
subject
Has Abstractpub_date
2005-02-11 00:00:00pages
7issn
1472-6750pii
1472-6750-5-7journal_volume
5pub_type
杂志文章abstract:BACKGROUND:Circular forms of viral genomic DNA are generated during infection of cells with retroviruses like HIV-1. Such circles are unable to replicate and are eventually lost as a result of cell division, lending support to the prevalent notion that episomal retroviral DNA forms are dead-end products of reverse tran...
journal_title:BMC biotechnology
pub_type: 杂志文章
doi:10.1186/1472-6750-8-60
更新日期:2008-08-09 00:00:00
abstract:BACKGROUND:Cymbidium faberi is one of the oldest cultivars of oriental orchids, with an elegant flower fragrance. In order to investigate the molecular mechanism and the functions of related proteins in the methyl jasmonate (MeJA) signaling pathway, one of the main components of flower fragrance in C. faberi, yeast one...
journal_title:BMC biotechnology
pub_type: 杂志文章
doi:10.1186/s12896-020-0599-2
更新日期:2020-01-16 00:00:00
abstract:BACKGROUND:Smoking and tobacco use continue to be the largest preventable causes of death globally. A novel therapeutic approach has recently been proposed: administration of an enzyme that degrades nicotine, the main addictive component of tobacco, minimizing brain exposure and reducing its reinforcing effects. Pre-cl...
journal_title:BMC biotechnology
pub_type: 杂志文章
doi:10.1186/s12896-019-0551-5
更新日期:2019-08-02 00:00:00
abstract:: ...
journal_title:BMC biotechnology
pub_type: 杂志文章
doi:10.1186/s12896-019-0504-z
更新日期:2019-02-18 00:00:00
abstract:BACKGROUND:The CRISPR/Cas9 system is increasingly used for gene inactivation in mouse zygotes, but homology-directed mutagenesis and use of inbred embryos are less established. In particular, Rosa26 knock-in alleles for the insertion of transgenes in a genomic 'safe harbor' site, have not been produced. Here we applied...
journal_title:BMC biotechnology
pub_type: 杂志文章
doi:10.1186/s12896-016-0234-4
更新日期:2016-01-16 00:00:00
abstract:BACKGROUND:Recombinant factor VIII (FVIII), used for haemophilia A therapy, is one of the most challenging among the therapeutic proteins produced in heterologous expression systems. Deletion variant of FVIII, in which the entire domain B is replaced by a short linker peptide, was approved for medical use. Efficacy and...
journal_title:BMC biotechnology
pub_type: 杂志文章
doi:10.1186/s12896-017-0353-6
更新日期:2017-03-24 00:00:00
abstract:BACKGROUND:Hydrophobic polystyrene is the most common material for solid phase immunoassay. Proteins are immobilized on polystyrene by passive adsorption, which often causes considerable denaturation. Biological macromolecules were found to better retain their functional activity when immobilized on hydrophilic materia...
journal_title:BMC biotechnology
pub_type: 杂志文章
doi:10.1186/1472-6750-1-4
更新日期:2001-01-01 00:00:00
abstract:BACKGROUND:Polysialic acid (polySia) is a carbohydrate modification of the neural cell adhesion molecule (NCAM), which is implicated in neural differentiation and plays an important role in tumor development and metastasis. Polysialylation of NCAM is mediated by two Golgi-resident polysialyltransferases (polyST) ST8Sia...
journal_title:BMC biotechnology
pub_type: 杂志文章
doi:10.1186/s12896-017-0360-7
更新日期:2017-05-12 00:00:00
abstract:BACKGROUND:Prostate cancer is the most-diagnosed non-skin cancer among males in the US, and the second leading cause of cancer-related death. Current methods of treatment and diagnosis are not specific for the disease. This work identified an antibody fragment that binds selectively to a molecule on the surface of andr...
journal_title:BMC biotechnology
pub_type: 杂志文章
doi:10.1186/1472-6750-14-81
更新日期:2014-09-03 00:00:00
abstract:BACKGROUND:Cost-effective production of industrially important enzymes is a key for their successful exploitation on industrial scale. Keeping in view the extensive industrial applications of lignin peroxidase (LiP), this study was performed to purify and characterize the LiP from an indigenous strain of Trametes versi...
journal_title:BMC biotechnology
pub_type: 杂志文章
doi:10.1186/1472-6750-12-46
更新日期:2012-08-03 00:00:00
abstract:BACKGROUND:Despite positive reports on the efficacy of stem cell therapy for the treatment of cardiovascular disease, the nature of stem cell homing to ischemic tissues remains elusive. RESULTS:We used a mouse model of peripheral tissue ischemia to study the survival and homing capacity of dual reporter gene (eGFP/Luc...
journal_title:BMC biotechnology
pub_type: 杂志文章
doi:10.1186/1472-6750-12-93
更新日期:2012-12-03 00:00:00
abstract:BACKGROUND:One of the most commonly used vectors for gene therapy is the adenoviral vector; its ability to tightly regulate transgene expression is critical for optimizing therapeutic outcomes. The tetracycline-regulated system (especially the Tet-On system) for gene expression is one of the most valuable tools for con...
journal_title:BMC biotechnology
pub_type: 杂志文章
doi:10.1186/s12896-015-0121-4
更新日期:2015-02-13 00:00:00
abstract:BACKGROUND:The detection of unknown mutations is important in research and medicine. For this purpose, a mismatch-specific endonuclease CEL I from celery has been established as a useful tool in high throughput projects. Previously, CEL I-like activities were described only in a variety of plants and could not be expre...
journal_title:BMC biotechnology
pub_type: 杂志文章
doi:10.1186/1472-6750-7-29
更新日期:2007-06-01 00:00:00
abstract:BACKGROUND:The therapeutic use of α-amidated peptides (e.g. calcitonin, glucagon-like peptide) has increased dramatically, but there are major impediments to wider use of such peptides. Larger peptides are expensive to synthesize, and short plasma half-lives frequently limit the clinical circumstances in which the pept...
journal_title:BMC biotechnology
pub_type: 杂志文章
doi:10.1186/s12896-015-0173-5
更新日期:2015-06-27 00:00:00
abstract:BACKGROUND:The identification of known mutations in a cell population is important for clinical applications and basic cancer research. In this work an immobilized form of the polymerase chain reaction, referred to as polony technology, was used to detect mutations as well as gene deletions, resulting in loss of hetero...
journal_title:BMC biotechnology
pub_type: 杂志文章
doi:10.1186/1472-6750-3-11
更新日期:2003-07-23 00:00:00
abstract:BACKGROUND:Bacterial degradation/transformation of steroids is widely investigated to create biotechnologically relevant strains for industrial application. The strain of Nocardioides simplex VKM Ac-2033D is well known mainly for its superior 3-ketosteroid Δ1-dehydrogenase activity towards various 3-oxosteroids and oth...
journal_title:BMC biotechnology
pub_type: 杂志文章
doi:10.1186/s12896-021-00668-9
更新日期:2021-01-13 00:00:00
abstract:BACKGROUND:This study aims to assess suitability of hydroponic technology for treatment of brewery wastewater in a hydroponic bioreactor using Typha latifolia. Triplicated hydroponic bioreactor treatment units were designed, constructed and operated at a hydraulic retention time of 5 days with different surface loading...
journal_title:BMC biotechnology
pub_type: 杂志文章
doi:10.1186/s12896-018-0484-4
更新日期:2018-11-22 00:00:00
abstract:BACKGROUND:With the increasing number of GMOs on the global market the maintenance of European GMO regulations is becoming more complex. For the analysis of a single food or feed sample it is necessary to assess the sample for the presence of many GMO-targets simultaneously at a sensitive level. Several methods have be...
journal_title:BMC biotechnology
pub_type: 杂志文章
doi:10.1186/1472-6750-12-4
更新日期:2012-01-19 00:00:00
abstract:BACKGROUND:The insect cell line is a critical component in the production of recombinant proteins in the baculovirus expression system and new cell lines hold the promise of increasing both quantity and quality of protein production. RESULTS:Seventy cell lines were established by single-cell cloning from a primary cul...
journal_title:BMC biotechnology
pub_type: 杂志文章
doi:10.1186/1472-6750-10-50
更新日期:2010-07-05 00:00:00
abstract:BACKGROUND:Xylanase can replace chemical additives to improve the volume and sensory properties of bread in the baking. Suitable baking xylanase with improved yield will promote the application of xylanase in baking industry. The xylanase XYNZG from the Plectosphaerella cucumerina has been previously characterized by h...
journal_title:BMC biotechnology
pub_type: 杂志文章
doi:10.1186/s12896-014-0107-7
更新日期:2014-12-16 00:00:00
abstract:BACKGROUND:The green fluorescent protein (GFP) has been regarded as a valuable tool and widely applied as a biomarker in medical applications and diagnostics. A cost-efficient upstream expression system and an inexpensive downstream purification process will meet the demands of the GFP protein with high-purity. RESULT...
journal_title:BMC biotechnology
pub_type: 杂志文章
doi:10.1186/s12896-019-0590-y
更新日期:2019-12-09 00:00:00
abstract:BACKGROUND:Construction of high quality cDNA libraries from the usually low amounts of eukaryotic mRNA extracted from environmental samples is essential in functional metatranscriptomics for the selection of functional, full-length genes encoding proteins of interest. Many of the inserts in libraries constructed by sta...
journal_title:BMC biotechnology
pub_type: 杂志文章
doi:10.1186/1472-6750-14-80
更新日期:2014-09-03 00:00:00
abstract:BACKGROUND:Many growth factors, such as bone morphogenetic protein (BMP)-2, have been shown to interact with polymers of sulfated disacharrides known as heparan sulfate (HS) glycosaminoglycans (GAGs), which are found on matrix and cell-surface proteoglycans throughout the body. HS GAGs, and some more highly sulfated fo...
journal_title:BMC biotechnology
pub_type: 杂志文章
doi:10.1186/1472-6750-12-60
更新日期:2012-09-11 00:00:00
abstract:BACKGROUND:In spite of a great number of monomeric fluorescent proteins developed in the recent years, the reported fluorescent protein-based FRET pairs are still characterized by a number of disadvantageous features, complicating their use as reporters in cell biology and for high-throughput cell-based screenings. RE...
journal_title:BMC biotechnology
pub_type: 杂志文章
doi:10.1186/1472-6750-9-24
更新日期:2009-03-25 00:00:00
abstract:BACKGROUND:The photorespiratory nitrogen cycle in C₃ plants involves an extensive diversion of carbon and nitrogen away from the direct pathways of assimilation. The liberated ammonia is re-assimilated, but up to 25% of the carbon may be released into the atmosphere as CO₂. Because of the loss of CO₂ and high energy co...
journal_title:BMC biotechnology
pub_type: 杂志文章
doi:10.1186/1472-6750-11-111
更新日期:2011-11-21 00:00:00
abstract:BACKGROUND:Over-expressed native or recombinant proteins are commonly used for industrial and pharmaceutical purposes, as well as for research. Proteins of interest need to be purified in sufficient quantity, quality and specific activity to justify their commercial price and eventual medical use. Proteome quality was ...
journal_title:BMC biotechnology
pub_type: 杂志文章
doi:10.1186/s12896-016-0259-8
更新日期:2016-03-11 00:00:00
abstract:BACKGROUND:Tetanus neurotoxin (TeNT) is taken up at nerve terminals and undergoes retrograde migration. The toxic properties of TeNT reside in the toxin light chain (L), but like complete TeNT, the TeNT heavy chain (TTH) and the C-terminal domain (TTC) alone can bind and enter into neurons. Here, we explored whether at...
journal_title:BMC biotechnology
pub_type: 杂志文章
doi:10.1186/s12896-018-0452-z
更新日期:2018-06-11 00:00:00
abstract:BACKGROUND:In view of compliance with increasingly stringent environmental legislation, an eco-friendly treatment technology of industrial dyes and effluents is a major environmental challenge in the color industry. In present study, a promising and eco-friendly entrapment approach was adopted to immobilize purified ma...
journal_title:BMC biotechnology
pub_type: 杂志文章
doi:10.1186/s12896-015-0227-8
更新日期:2015-12-10 00:00:00
abstract:BACKGROUND:Splicing of DNA molecules is an important task in molecular biology that facilitates cloning, mutagenesis and creation of chimeric genes. Mutagenesis and DNA splicing techniques exist, some requiring restriction enzymes, and others utilize staggered reannealing approaches. RESULTS:A method for DNA splicing ...
journal_title:BMC biotechnology
pub_type: 杂志文章
doi:10.1186/1472-6750-5-23
更新日期:2005-09-01 00:00:00
abstract:BACKGROUND:Mesenchymal stem cells (MSCs) are an attractive source of adult stem cells for therapeutic application in clinical study. Genetic modification of MSCs with beneficial genes makes them more effective for therapeutic use. However, it is difficult to transduce genes into MSCs by common transfection methods, esp...
journal_title:BMC biotechnology
pub_type: 杂志文章
doi:10.1186/1472-6750-10-38
更新日期:2010-05-13 00:00:00