Abstract:
BACKGROUND:Microarray technology has been used extensively over the past 10 years for assessing gene expression, and has facilitated precise genetic profiling of everything from tumors to small molecule drugs. By contrast, arraying cell membranes in a manner which preserves their ability to mediate biochemical processes has been considerably more difficult. RESULTS:In this article, we describe a novel technology for generating cell membrane microarrays for performing high throughput biology. Our robotically-arrayed supported membranes are physiologically fluid, a critical property which differentiates this technology from other previous membrane systems and makes it useful for studying cellular processes on an industrialized scale. Membrane array elements consist of a solid substrate, above which resides a fluid supported lipid bilayer containing biologically-active molecules of interest. Incorporation of transmembrane proteins into the arrayed membranes enables the study of ligand/receptor binding, as well as interactions with live intact cells. The fluidity of these molecules in the planar lipid bilayer facilitates dimerization and other higher order interactions necessary for biological signaling events. In order to demonstrate the utility of our fluid membrane array technology to ligand/receptor studies, we investigated the multivalent binding of the cholera toxin B-subunit (CTB) to the membrane ganglioside GM1. We have also displayed a number of bona fide drug targets, including bacterial endotoxin (also referred to as lipopolysaccharide (LPS)) and membrane proteins important in T cell activation. CONCLUSION:We have demonstrated the applicability of our fluid cell membrane array technology to both academic research applications and industrial drug discovery. Our technology facilitates the study of ligand/receptor interactions and cell-cell signaling, providing rich qualitative and quantitative information.
journal_name
BMC Biotechnoljournal_title
BMC biotechnologyauthors
Yamazaki V,Sirenko O,Schafer RJ,Nguyen L,Gutsmann T,Brade L,Groves JTdoi
10.1186/1472-6750-5-18keywords:
subject
Has Abstractpub_date
2005-06-16 00:00:00pages
18issn
1472-6750pii
1472-6750-5-18journal_volume
5pub_type
杂志文章abstract:BACKGROUND:Construction of high quality cDNA libraries from the usually low amounts of eukaryotic mRNA extracted from environmental samples is essential in functional metatranscriptomics for the selection of functional, full-length genes encoding proteins of interest. Many of the inserts in libraries constructed by sta...
journal_title:BMC biotechnology
pub_type: 杂志文章
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更新日期:2017-07-04 00:00:00
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abstract:: ...
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journal_title:BMC biotechnology
pub_type: 杂志文章
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journal_title:BMC biotechnology
pub_type: 杂志文章
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pub_type: 杂志文章
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