Ultrasound and microbubble induced release from intracellular compartments.

abstract：BACKGROUND:Genetically encoded sensors developed on the basis of green fluorescent protein (GFP)-like proteins are becoming more and more popular instruments for monitoring cellular analytes and enzyme activities in living cells and transgenic organisms. In particular, a number of Ca2+ sensors have been developed, eith...

journal_title：BMC biotechnology

authors： Souslova EA,Belousov VV,Lock JG,Strömblad S,Kasparov S,Bolshakov AP,Pinelis VG,Labas YA,Lukyanov S,Mayr LM,Chudakov DM

更新日期：2007-06-29 00:00:00

abstract：BACKGROUND:The ability to produce the same recombinant protein in both prokaryotic and eukaryotic cells offers many experimental opportunities. However, the cloning of the same gene into multiple plasmids is required, which is time consuming, laborious and still may not produce soluble, stable protein in sufficient qua...

journal_title：BMC biotechnology

authors： Sinah N,Williams CA,Piper RC,Shields SB

更新日期：2012-08-23 00:00:00

abstract：BACKGROUND:Bacterial degradation/transformation of steroids is widely investigated to create biotechnologically relevant strains for industrial application. The strain of Nocardioides simplex VKM Ac-2033D is well known mainly for its superior 3-ketosteroid Δ1-dehydrogenase activity towards various 3-oxosteroids and oth...

journal_title：BMC biotechnology

authors： Shtratnikova VY,Sсhelkunov MI,Fokina VV,Bragin EY,Shutov AA,Donova MV

更新日期：2021-01-13 00:00:00

abstract：BACKGROUND:Urease B is an important virulence factor that is required for Helicobacter pylori to colonise the gastric mucosa. Mouse monoclonal antibodies (mAbs) that inhibit urease B enzymatic activity will be useful as vaccines for the prevention and treatment of H. pylori infection. Here, we produced murine mAbs agai...

journal_title：BMC biotechnology

authors： Li Y,Ning Y,Wang Y,Peng D,Jiang Y,Zhang L,Long M,Luo J,Li M

更新日期：2010-11-30 00:00:00

abstract：BACKGROUND:The isothermal amplification of RNA in vitro has been used for the study of in vitro evolution of RNA. Although Qβ replicase has been traditionally used as an enzyme for this purpose, we planned to use norovirus replicase (NV3D(pol)) due to its structural simplicity in the scope of in vitro autonomous evolut...

journal_title：BMC biotechnology

authors： Arai H,Nishigaki K,Nemoto N,Suzuki M,Husimi Y

更新日期：2013-10-09 00:00:00

abstract：BACKGROUND:Baculovirus, which has a width of 40 nm and a length of 250-300 nm, can display functional peptides, receptors and antigens on its surface by their fusion with a baculovirus envelop protein, GP64. In addition, some transmembrane proteins can be displayed without GP64 fusion, using the native transmembrane do...

journal_title：BMC biotechnology

authors： Kato T,Suzuki F,Park EY

更新日期：2011-06-02 00:00:00

abstract：BACKGROUND:β-Fructofuranosidases (or invertases) catalyse the commercially-important biotransformation of sucrose into short-chain fructooligosaccharides with wide-scale application as a prebiotic in the functional foods and pharmaceutical industries. RESULTS:We identified a β-fructofuranosidase gene (CmINV) from a Ce...

journal_title：BMC biotechnology

authors： van Wyk N,Trollope KM,Steenkamp ET,Wingfield BD,Volschenk H

更新日期：2013-11-14 00:00:00

abstract：BACKGROUND:Xenoantigens are a major source of concern with regard to the success of interspecific xenografts. GGTA1 encodes α1,3-galactosyltransferase, which is essential for the biosynthesis of galactosyl-alpha 1,3-galactose, the major xenoantigen causing hyperacute rejection. GGTA1-modified pigs, therefore, are promi...

journal_title：BMC biotechnology

authors： Tanihara F,Hirata M,Nguyen NT,Sawamoto O,Kikuchi T,Doi M,Otoi T

更新日期：2020-08-18 00:00:00

abstract：BACKGROUND:The cattle ticks, Boophilus spp., affect cattle production in tropical and subtropical regions of the world. Tick vaccines constitute a cost-effective and environmentally friendly alternative to tick control. The recombinant B. microplus Bm86 protective antigen has been shown to protect cattle against tick i...

journal_title：BMC biotechnology

authors： Canales M,Almazán C,Naranjo V,Jongejan F,de la Fuente J

更新日期：2009-03-31 00:00:00

abstract：BACKGROUND:Mesenchymal stem cells (MSCs) are of great interest in bone regenerative medicine due to their osteogenic potential and trophic effects. However, challenges to large-scale production of MSCs can hinder the translation of MSC therapies. 3D Microcarrier (MC)-based MSC culture presents a scalable and cost-effec...

journal_title：BMC biotechnology

authors： Shekaran A,Sim E,Tan KY,Chan JK,Choolani M,Reuveny S,Oh S

更新日期：2015-10-31 00:00:00

abstract：BACKGROUND:Ligand-targeted approaches have proven successful in improving the therapeutic index of a number of drugs. We hypothesized that the specific targeting of TNF-alpha antagonists to inflamed tissues could increase drug efficacy and reduce side effects. RESULTS:Using uteroglobin (UG), a potent anti-inflammatory...

journal_title：BMC biotechnology

authors： Ventura E,Balza E,Borsi L,Tutolo G,Carnemolla B,Castellani P,Zardi L

更新日期：2011-11-10 00:00:00

abstract：BACKGROUND:High recombinant protein productivity in mammalian cell lines is often associated with phenotypic changes in protein content, energy metabolism, and cell growth, but the key determinants that regulate productivity are still not clearly understood. The mammalian target of rapamycin (mTOR) signalling pathway h...

journal_title：BMC biotechnology

authors： Edros R,McDonnell S,Al-Rubeai M

更新日期：2014-02-17 00:00:00

abstract：BACKGROUND:There are significant differences in the culture conditions between small-scale screenings and large-scale fermentation processes. Production processes are usually conducted in fed-batch cultivation mode with active pH-monitoring and control. In contrast, screening experiments in shake flasks are usually con...

journal_title：BMC biotechnology

authors： Scheidle M,Dittrich B,Klinger J,Ikeda H,Klee D,Büchs J

更新日期：2011-03-23 00:00:00

abstract：BACKGROUND:Over-expressed native or recombinant proteins are commonly used for industrial and pharmaceutical purposes, as well as for research. Proteins of interest need to be purified in sufficient quantity, quality and specific activity to justify their commercial price and eventual medical use. Proteome quality was ...

journal_title：BMC biotechnology

authors： Musa M,Radman M,Krisko A

更新日期：2016-03-11 00:00:00

abstract：BACKGROUND:Increasing cell membrane permeability to water and cryoprotectants is critical for the successful cryopreservation of cells with large volumes. Artificial expression of water-selective aquaporins or aquaglyceroporins (GLPs), such as mammalian aquaporin-3 (AQP3), enhances cell permeability to water and cryopr...

journal_title：BMC biotechnology

authors： Chauvigné F,Lubzens E,Cerdà J

更新日期：2011-04-08 00:00:00

abstract：BACKGROUND:At first glance, there appears to be a high degree of similarity between the metabolism of yeast (the Crabtree effect) and human cancer cells (the Warburg effect). At the root of both effects is accelerated metabolic flow through glycolysis which leads to overflows of ethanol and lactic acid, respectively. I...

journal_title：BMC biotechnology

authors： Andrejc D,Možir A,Legiša M

更新日期：2017-05-08 00:00:00

abstract：BACKGROUND:In a previous study, anthocyanin levels in potato plants were increased by manipulating genes connected with the flavonoid biosynthesis pathway. However, starch content and tuber yield were dramatically reduced in the transgenic plants, which over-expressed dihydroflavonol reductase (DFR). RESULTS:Transgeni...

journal_title：BMC biotechnology

authors： Aksamit-Stachurska A,Korobczak-Sosna A,Kulma A,Szopa J

更新日期：2008-03-05 00:00:00

abstract：BACKGROUND:Loop mediated isothermal amplification of nucleic acid templates is a rapid, sensitive and specific method suitable for molecular diagnostics. However the complexity of primer design and the number of primers involved can lead to false positives from non-specific primer interactions. Standard methods of LAMP...

journal_title：BMC biotechnology

authors： Hardinge P,Murray JAH

更新日期：2019-08-01 00:00:00

abstract：BACKGROUND:The recent H1N1 influenza pandemic illustrated the shortcomings of the vaccine manufacturing process. The A/California/07/2009 H1N1 pandemic influenza vaccine or A(H1N1)pdm09 was available late and in short supply as a result of delays in production caused by low yields and poor antigen stability. Recombinan...

journal_title：BMC biotechnology

authors： Feshchenko E,Rhodes DG,Felberbaum R,McPherson C,Rininger JA,Post P,Cox MM

更新日期：2012-10-30 00:00:00

abstract：BACKGROUND:Heparan sulfate glycosaminoglycans are diverse components of certain proteoglycans and are known to interact with growth factors as a co-receptor necessary to induce signalling and growth factor activity. In this report we characterize heterogeneously glycosylated recombinant human perlecan domain 1 (HSPG2 a...

journal_title：BMC biotechnology

authors： Ellis AL,Pan W,Yang G,Jones K,Chuang C,Whitelock JM,DeCarlo AA

更新日期：2010-09-09 00:00:00

abstract：BACKGROUND:Plants may represent excellent alternatives to classical heterologous protein expression systems, especially for the production of biopharmaceuticals and vaccine components. Modern vaccines are becoming increasingly complex, with the incorporation of multiple antigens. Approaches towards developing an HIV va...

journal_title：BMC biotechnology

authors： Marusic C,Nuttall J,Buriani G,Lico C,Lombardi R,Baschieri S,Benvenuto E,Frigerio L

更新日期：2007-02-26 00:00:00

abstract：BACKGROUND:The proteomics literature has seen a proliferation of publications that seek to apply the rapidly improving technology of 2D gels to study various biological systems. However, there is a dearth of systematic studies that have investigated appropriate statistical approaches to analyse the data from these expe...

journal_title：BMC biotechnology

authors： Meleth S,Deshane J,Kim H

更新日期：2005-02-11 00:00:00

abstract：BACKGROUND:The CRISPR/Cas9 genome editing system has greatly facilitated and expanded our capacity to engineer mammalian genomes, including targeted gene knock-outs. However, the phenotyping of the knock-out effect requires a high DNA editing efficiency. RESULTS:Here, we report a user-friendly strategy based on the ex...

journal_title：BMC biotechnology

authors： Liesche C,Venkatraman L,Aschenbrenner S,Grosse S,Grimm D,Eils R,Beaudouin J

更新日期：2016-02-16 00:00:00

abstract：BACKGROUND:CRISPR/Cas9 is widely used for precise genetic editing in various organisms. CRISPR/Cas9 editing may in many plants be hampered by the presence of complex and high ploidy genomes and inefficient or poorly controlled delivery of the CRISPR/Cas9 components to gamete cells or cells with regenerative potential. ...

journal_title：BMC biotechnology

authors： Petersen BL,Möller SR,Mravec J,Jørgensen B,Christensen M,Liu Y,Wandall HH,Bennett EP,Yang Z

更新日期：2019-06-17 00:00:00

abstract：BACKGROUND:Anti-NMDA receptor encephalitis (ANRE) is a potentially lethal disease attributed to auto-antibodies against the N-methyl-D-aspartate receptor (NMDAR). Full recovery is possible if therapy is initiated early in the disease course. Detection of ANRE antibodies in the cerebrospinal fluid (CSF) is essential for...

journal_title：BMC biotechnology

authors： Sharma R,Al-Saleem FH,Puligedda RD,Rattelle A,Lynch DR,Dessain SK

更新日期：2018-06-27 00:00:00

abstract：BACKGROUND:More than a dozen of fungal immunomodulatory proteins (FIPs) have been identified to date, most of which are from Ganoderma species. However, little is known about the similarities and differences between different Ganoderma FIPs' bioactivities. In the current study, two FIP genes termed FIP-gap1 and FIP-gap...

journal_title：BMC biotechnology

authors： Qu ZW,Zhou SY,Guan SX,Gao R,Duan ZW,Zhang X,Sun WY,Fan WL,Chen SS,Chen LJ,Lin JW,Ruan YY

更新日期：2018-12-14 00:00:00

abstract：BACKGROUND:Understanding and manipulating gene function in physiological conditions is a major objective for both fundamental and applied research. In contrast to other experimental settings, which use either purely genetic or gene delivery (viral or non-viral) strategies, we report here a strategy based on direct prot...

journal_title：BMC biotechnology

authors： Gitton Y,Tibaldi L,Dupont E,Levi G,Joliot A

更新日期：2009-04-24 00:00:00

abstract：BACKGROUND:The ketoisovalerate reductase (EC 1.2.7.7 ) is required for the formation of beauvericin via the nonribosomal peptide synthetase biosynthetic pathway. It catalyzes the NADPH-specific reduction of ketoisovaleric acid to hydroxyisovalerate. However, little is known about the bioinformatics' data about the 2-Ki...

journal_title：BMC biotechnology

authors： Zhang T,Jia X,Zhuo Y,Liu M,Gao H,Liu J,Zhang L

更新日期：2012-08-23 00:00:00

abstract：BACKGROUND:The CRISPR/Cas9 system is increasingly used for gene inactivation in mouse zygotes, but homology-directed mutagenesis and use of inbred embryos are less established. In particular, Rosa26 knock-in alleles for the insertion of transgenes in a genomic 'safe harbor' site, have not been produced. Here we applied...

journal_title：BMC biotechnology

authors： Chu VT,Weber T,Graf R,Sommermann T,Petsch K,Sack U,Volchkov P,Rajewsky K,Kühn R

更新日期：2016-01-16 00:00:00

abstract：BACKGROUND:Microarray technology has been used extensively over the past 10 years for assessing gene expression, and has facilitated precise genetic profiling of everything from tumors to small molecule drugs. By contrast, arraying cell membranes in a manner which preserves their ability to mediate biochemical processe...

journal_title：BMC biotechnology

authors： Yamazaki V,Sirenko O,Schafer RJ,Nguyen L,Gutsmann T,Brade L,Groves JT

更新日期：2005-06-16 00:00:00