Abstract:
BACKGROUND:Arachidonic acid (ARA, C20:4, n-6), which belongs to the omega-6 series of polyunsaturated fatty acids and has a variety of biological activities, is commercially produced in Mortierella alpina. Dissolved oxygen or oxygen utilization efficiency is a critical factor for Mortierella alpina growth and arachidonic acid production in large-scale fermentation. Overexpression of the Vitreoscilla hemoglobin gene is thought to significantly increase the oxygen utilization efficiency of the cells. RESULTS:An optimized Vitreoscilla hemoglobin (VHb) gene was introduced into Mortierella alpina via Agrobacterium tumefaciens-mediated transformation. Compared with the parent strain, the VHb-expressing strain, termed VHb-20, grew faster under both limiting and non-limiting oxygen conditions and exhibited dramatic changes in cell morphology. Furthermore, VHb-20 produced 4- and 8-fold higher total lipid and ARA yields than those of the wild-type strain under a microaerobic environment. Furthermore, ARA production of VHb-20 was also 1.6-fold higher than that of the wild type under normal conditions. The results demonstrated that DO utilization was significantly increased by expressing the VHb gene in Mortierella alpina. CONCLUSIONS:The expression of VHb enhances ARA and lipid production under both lower and normal dissolved oxygen conditions. This study provides a novel strategy and an engineered strain for the cost-efficient production of ARA.
journal_name
BMC Biotechnoljournal_title
BMC biotechnologyauthors
Zhang H,Feng Y,Cui Q,Song Xdoi
10.1186/s12896-017-0388-8subject
Has Abstractpub_date
2017-08-30 00:00:00pages
68issue
1issn
1472-6750pii
10.1186/s12896-017-0388-8journal_volume
17pub_type
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journal_title:BMC biotechnology
pub_type: 杂志文章
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journal_title:BMC biotechnology
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journal_title:BMC biotechnology
pub_type: 杂志文章
doi:10.1186/1472-6750-6-8
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