Keratinocyte cytoskeletal roles in cell sheet engineering.

abstract：BACKGROUND:Acetyl-xylan esterase (AXE, EC 3.1.1.72) hydrolyses acetate group from the linear chain of xylopyranose residues bound by β-1,4-linkage. The enzyme finds commercial applications in bio-bleaching of wood pulp, treating animal feed to increase digestibility, processing food to increase clarification and conver...

journal_title：BMC biotechnology

authors： Atta S,Ali S,Akhtar MN,Haq I

更新日期：2011-05-16 00:00:00

abstract：BACKGROUND:In the previous study, the cellulolytic Escherichia coli ZH-4 isolated from bovine rumen was found to show extracellular cellulase activity and could degrade cellulose in the culture. The goal of this work was to identify and characterize the secreted cellulase of E. coli ZH-4. It will be helpful to re-under...

journal_title：BMC biotechnology

authors： Pang J,Wang J,Liu Z,Zhang Q,Qi Q

更新日期：2019-08-27 00:00:00

abstract：BACKGROUND:Methylated CpG dinucleotides in promoters are associated with the loss of gene expression in recombinant Chinese hamster ovary (CHO) cells during large-scale commercial manufacturing. We evaluated a promoter devoid of CpG dinucleotides, CpGfree, in parallel with a similar CpG containing promoter, CpGrich, fo...

journal_title：BMC biotechnology

authors： Ho SC,Koh EY,Soo BP,Mariati,Chao SH,Yang Y

更新日期：2016-10-18 00:00:00

abstract：BACKGROUND:Interest in using plants for production of recombinant proteins such as monoclonal antibodies is growing, but proteolytic degradation, leading to a loss of functionality and complications in downstream purification, is still a serious problem. RESULTS:In this study, we investigated the dynamics of the assem...

journal_title：BMC biotechnology

authors： Hehle VK,Paul MJ,Drake PM,Ma JK,van Dolleweerd CJ

更新日期：2011-12-30 00:00:00

abstract：BACKGROUND:Mesenchymal stem cells (MSCs) are of great interest in bone regenerative medicine due to their osteogenic potential and trophic effects. However, challenges to large-scale production of MSCs can hinder the translation of MSC therapies. 3D Microcarrier (MC)-based MSC culture presents a scalable and cost-effec...

journal_title：BMC biotechnology

authors： Shekaran A,Sim E,Tan KY,Chan JK,Choolani M,Reuveny S,Oh S

更新日期：2015-10-31 00:00:00

abstract：BACKGROUND:Clostridial co-culture containing cellulolytic and solventogenic species is a potential consolidated bioprocessing (CBP) approach for producing biochemicals and biofuels from cellulosic biomass. It has been demonstrated that the rate of cellulose utilization in the co-culture of Clostridium acetobutylicum an...

journal_title：BMC biotechnology

authors： Salimi F,Mahadevan R

更新日期：2013-11-04 00:00:00

abstract：BACKGROUND:Tetanus neurotoxin (TeNT) is taken up at nerve terminals and undergoes retrograde migration. The toxic properties of TeNT reside in the toxin light chain (L), but like complete TeNT, the TeNT heavy chain (TTH) and the C-terminal domain (TTC) alone can bind and enter into neurons. Here, we explored whether at...

journal_title：BMC biotechnology

authors： Watanabe Y,Matsuba T,Nakanishi M,Une M,Hanajima R,Nakashima K

更新日期：2018-06-11 00:00:00

abstract：BACKGROUND:Human TGF-β3 has been used in many studies to induce genes coding for typical cartilage matrix components and accelerate chondrogenic differentiation, making it the standard constituent in most cultivation media used for the assessment of chondrogenesis associated with various stem cell types on carrier matr...

journal_title：BMC biotechnology

authors： Huang Y,Seitz D,Chevalier Y,Müller PE,Jansson V,Klar RM

更新日期：2020-08-27 00:00:00

abstract：BACKGROUND:The cattle ticks, Boophilus spp., affect cattle production in tropical and subtropical regions of the world. Tick vaccines constitute a cost-effective and environmentally friendly alternative to tick control. The recombinant B. microplus Bm86 protective antigen has been shown to protect cattle against tick i...

journal_title：BMC biotechnology

authors： Canales M,Almazán C,Naranjo V,Jongejan F,de la Fuente J

更新日期：2009-03-31 00:00:00

abstract：BACKGROUND:Transformed cells of Escherichia coli DH5-alpha with pGFPuv, induced by IPTG (isopropyl-beta-d-thiogalactopyranoside), express the green fluorescent protein (gfpuv) during growth phases. E. coli subjected to the combination of selective permeation by freezing/thawing/sonication cycles followed by the three-p...

journal_title：BMC biotechnology

authors： Vessoni Penna TC,Ishii M

更新日期：2002-04-24 00:00:00

abstract：BACKGROUND:Construction of high quality cDNA libraries from the usually low amounts of eukaryotic mRNA extracted from environmental samples is essential in functional metatranscriptomics for the selection of functional, full-length genes encoding proteins of interest. Many of the inserts in libraries constructed by sta...

journal_title：BMC biotechnology

authors： Yadav RK,Barbi F,Ziller A,Luis P,Marmeisse R,Reddy MS,Fraissinet-Tachet L

更新日期：2014-09-03 00:00:00

abstract：BACKGROUND:The recent H1N1 influenza pandemic illustrated the shortcomings of the vaccine manufacturing process. The A/California/07/2009 H1N1 pandemic influenza vaccine or A(H1N1)pdm09 was available late and in short supply as a result of delays in production caused by low yields and poor antigen stability. Recombinan...

journal_title：BMC biotechnology

authors： Feshchenko E,Rhodes DG,Felberbaum R,McPherson C,Rininger JA,Post P,Cox MM

更新日期：2012-10-30 00:00:00

abstract：BACKGROUND:Vascular endothelial growth factor (VEGF) is a key angiogenic factors. It plays an important role in both physiologic and pathologic angiogenesis and increases permeability across the vessels. Using antibody phage display technology, we obtained a novel anti-VEGFA IgG, named as FD006. In this study, the phar...

journal_title：BMC biotechnology

authors： Wang Q,Yang J,Tang K,Luo L,Wang L,Tian L,Jiang Y,Feng J,Li Y,Shen B,Lv M,Huang Y

更新日期：2014-02-27 00:00:00

abstract：BACKGROUND:The ability of cytosine deaminase (CD) to convert the antifungal agent 5-fluorocytosine (5-FC) into one of the most potent and largely used anticancer compound such as 5-fluorouracil (5-FU) raised considerable interest in this enzyme to model gene or antibody - directed enzyme-prodrug therapy (GDEPT/ADEPT) a...

journal_title：BMC biotechnology

authors： Mallano A,Zamboni S,Carpinelli G,Santoro F,Flego M,Ascione A,Gellini M,Tombesi M,Podo F,Cianfriglia M

更新日期：2008-09-10 00:00:00

abstract：BACKGROUND:MicroRNAs are important regulators of gene expression at the post-transcriptional level and play an important role in many biological processes. Due to the important biological role it is of great interest to quantitatively determine their expression level in different biological settings. RESULTS:We descri...

journal_title：BMC biotechnology

authors： Balcells I,Cirera S,Busk PK

更新日期：2011-06-25 00:00:00

abstract：BACKGROUND:von Willebrand factor (VWF) is a key load bearing domain for mamalian cell adhesion by binding various macromolecular ligands in extracellular matrix such as, collagens, elastin, and glycosaminoglycans. Interestingly, vWF like domains are also commonly found in load bearing systems of marine organisms such a...

journal_title：BMC biotechnology

authors： Yoo HY,Song YH,Foo M,Seo E,Hwang DS,Seo JH

更新日期：2016-02-16 00:00:00

abstract：BACKGROUND:The isothermal amplification of RNA in vitro has been used for the study of in vitro evolution of RNA. Although Qβ replicase has been traditionally used as an enzyme for this purpose, we planned to use norovirus replicase (NV3D(pol)) due to its structural simplicity in the scope of in vitro autonomous evolut...

journal_title：BMC biotechnology

authors： Arai H,Nishigaki K,Nemoto N,Suzuki M,Husimi Y

更新日期：2013-10-09 00:00:00

abstract：BACKGROUND:Whole genome amplification (WGA) promises to eliminate practical molecular genetic analysis limitations associated with genomic DNA (gDNA) quantity. We evaluated the performance of multiple displacement amplification (MDA) WGA using gDNA extracted from lymphoblastoid cell lines (N = 27) with a range of start...

journal_title：BMC biotechnology

authors： Bergen AW,Qi Y,Haque KA,Welch RA,Chanock SJ

更新日期：2005-09-16 00:00:00

abstract：BACKGROUND:Rapid, robust and reversible induction of transgene expression would significantly facilitate cancer gene therapy as well as allow the in vivo functional study of newly discovered genes in tumor formation and progression. The popularity of the ecdysone inducible gene switch system has led us to investigate w...

journal_title：BMC biotechnology

authors： Karns LR,Kisielewski A,Gulding KM,Seraj JM,Theodorescu D

更新日期：2001-01-01 00:00:00

abstract：BACKGROUND:Chinese hamster ovary (CHO) cells have become the host of choice for the production of recombinant proteins, due to their capacity for correct protein folding, assembly, and posttranslational modifications. The most widely used system for recombinant proteins is the gene amplification procedure that uses the...

journal_title：BMC biotechnology

authors： Jamnikar U,Nikolic P,Belic A,Blas M,Gaser D,Francky A,Laux H,Blejec A,Baebler S,Gruden K

更新日期：2015-10-23 00:00:00

abstract：BACKGROUND:Engineered proteins, with non-immunoglobulin scaffolds, have become an important alternative to antibodies in many biotechnical and therapeutic applications. When compared to antibodies, tailored proteins may provide advantageous properties such as a smaller size or a more stable structure. RESULTS:Avidin i...

journal_title：BMC biotechnology

authors： Riihimäki TA,Hiltunen S,Rangl M,Nordlund HR,Määttä JA,Ebner A,Hinterdorfer P,Kulomaa MS,Takkinen K,Hytönen VP

更新日期：2011-06-09 00:00:00

abstract：BACKGROUND:We have investigated the possibility and feasibility of producing the HPV-11 L1 major capsid protein in transgenic Arabidopsis thaliana ecotype Columbia and Nicotiana tabacum cv. Xanthi as potential sources for an inexpensive subunit vaccine. RESULTS:Transformation of plants was only achieved with the HPV-1...

journal_title：BMC biotechnology

authors： Kohl TO,Hitzeroth II,Christensen ND,Rybicki EP

更新日期：2007-09-12 00:00:00

abstract：BACKGROUND:The ubiquitin protein is present in all eukaryotic cells and promoters from ubiquitin genes are good candidates to regulate the constitutive expression of transgenes in plants. Therefore, two switchgrass (Panicum virgatum L.) ubiquitin genes (PvUbi1 and PvUbi2) were cloned and characterized. Reporter constru...

journal_title：BMC biotechnology

authors： Mann DG,King ZR,Liu W,Joyce BL,Percifield RJ,Hawkins JS,LaFayette PR,Artelt BJ,Burris JN,Mazarei M,Bennetzen JL,Parrott WA,Stewart CN Jr

更新日期：2011-07-11 00:00:00

abstract：BACKGROUND:Each year, influenza is responsible for hundreds of thousand cases of illness and deaths worldwide. Due to the virus' fast mutation rate, the World Health Organization (WHO) is constantly on alert to rapidly respond to emerging pandemic strains. Although anti-viral therapies exist, the most proficient way to...

journal_title：BMC biotechnology

authors： Thompson CM,Petiot E,Mullick A,Aucoin MG,Henry O,Kamen AA

更新日期：2015-05-16 00:00:00

abstract：BACKGROUND:Dengue is a public health problem of global significance for which there is neither an effective antiviral therapy nor a preventive vaccine. It is a mosquito-borne viral disease, caused by dengue (DEN) viruses, which are members of the Flaviviridae family. There are four closely related serotypes, DEN-1, DEN...

journal_title：BMC biotechnology

authors： Khanam S,Rajendra P,Khanna N,Swaminathan S

更新日期：2007-02-15 00:00:00

abstract：BACKGROUND:The extracellular domain of matrix protein 2 (M2e) of influenza A virus is a promising target for the development of a universal vaccine against influenza because M2e sequences are highly conserved among human influenza A strains. However, native M2e is poorly immunogenic, but its immunogenicity can be incre...

journal_title：BMC biotechnology

authors： Mardanova ES,Kotlyarov RY,Kuprianov VV,Stepanova LA,Tsybalova LM,Lomonosoff GP,Ravin NV

更新日期：2015-05-29 00:00:00

abstract：BACKGROUND:A method for inhibiting the expression of particular genes using external guide sequences (EGSs) has been developed in bacteria, mammalian cells and maize cells. RESULTS:To examine whether EGS technology can be used to down-regulate gene expression in Caenorhabditis elegans (C. elegans), we generated EGS-Ng...

journal_title：BMC biotechnology

authors： Yan Q,Zhao R,Zheng W,Yin C,Zhang B,Ma W

更新日期：2009-05-20 00:00:00

abstract：BACKGROUND:Carboxyl-terminal protease (CtpA) plays essential functions in posttranslational protein processing in prokaryotic and eukaryotic cells. To date, only a few bacterial ctpA genes have been characterized. Here we cloned and characterized a novel CtpA. The encoding gene, ctpAp (ctpA of Paenibacillus lautus), wa...

journal_title：BMC biotechnology

authors： Li Y,Pan Y,She Q,Chen L

更新日期：2013-10-25 00:00:00

abstract：BACKGROUND:The use of small interfering RNA (siRNA) molecules in animals to achieve double-stranded RNA-mediated interference (RNAi) has recently emerged as a powerful method of sequence-specific gene knockdown. As DNA-based expression of short hairpin RNA (shRNA) for RNAi may offer some advantages over chemical and in...

journal_title：BMC biotechnology

authors： Lambeth LS,Moore RJ,Muralitharan M,Dalrymple BP,McWilliam S,Doran TJ

更新日期：2005-05-11 00:00:00

abstract：BACKGROUND:At first glance, there appears to be a high degree of similarity between the metabolism of yeast (the Crabtree effect) and human cancer cells (the Warburg effect). At the root of both effects is accelerated metabolic flow through glycolysis which leads to overflows of ethanol and lactic acid, respectively. I...

journal_title：BMC biotechnology

authors： Andrejc D,Možir A,Legiša M

更新日期：2017-05-08 00:00:00