Characterization of norovirus RNA replicase for in vitro amplification of RNA.

abstract：BACKGROUND:Cymbidium faberi is one of the oldest cultivars of oriental orchids, with an elegant flower fragrance. In order to investigate the molecular mechanism and the functions of related proteins in the methyl jasmonate (MeJA) signaling pathway, one of the main components of flower fragrance in C. faberi, yeast one...

journal_title：BMC biotechnology

authors： Xu Y,Zhou J,Liu Q,Li K,Zhou Y

更新日期：2020-01-16 00:00:00

abstract：BACKGROUND:Expressed Sequence Tag (EST) sequences are widely used in applications such as genome annotation, gene discovery and gene expression studies. However, some of GenBank dbEST sequences have proven to be "unclean". Identification of cDNA termini/ends and their structures in raw ESTs not only facilitates data qu...

journal_title：BMC biotechnology

authors： Zhou S,Ji G,Liu X,Li P,Moler J,Karro JE,Liang C

更新日期：2012-05-03 00:00:00

abstract：BACKGROUND:Transformed cells of Escherichia coli DH5-alpha with pGFPuv, induced by IPTG (isopropyl-beta-d-thiogalactopyranoside), express the green fluorescent protein (gfpuv) during growth phases. E. coli subjected to the combination of selective permeation by freezing/thawing/sonication cycles followed by the three-p...

journal_title：BMC biotechnology

authors： Vessoni Penna TC,Ishii M

更新日期：2002-04-24 00:00:00

abstract：BACKGROUND:The use of small interfering RNA (siRNA) molecules in animals to achieve double-stranded RNA-mediated interference (RNAi) has recently emerged as a powerful method of sequence-specific gene knockdown. As DNA-based expression of short hairpin RNA (shRNA) for RNAi may offer some advantages over chemical and in...

journal_title：BMC biotechnology

authors： Lambeth LS,Moore RJ,Muralitharan M,Dalrymple BP,McWilliam S,Doran TJ

更新日期：2005-05-11 00:00:00

abstract：BACKGROUND:The use of highly reproducible and spatiallyhomogeneous thin film matrices permits automated microscopy and quantitative determination of the response of hundreds of cells in a population. Using thin films of extracellular matrix proteins, we have quantified, on a cell-by-cell basis, phenotypic parameters of...

journal_title：BMC biotechnology

authors： Langenbach KJ,Elliott JT,Tona A,McDaniel D,Plant AL

更新日期：2006-03-06 00:00:00

abstract：BACKGROUND:Alkaline phosphatase (AP) catalyzes the hydrolytic cleavage of phosphate monoesters under alkaline conditions and plays important roles in microbial ecology and molecular biology applications. Here, we report on the first isolation and biochemical characterization of a thermolabile AP from a metagenome. RES...

journal_title：BMC biotechnology

authors： Lee DH,Choi SL,Rha E,Kim SJ,Yeom SJ,Moon JH,Lee SG

更新日期：2015-01-31 00:00:00

abstract：BACKGROUND:Urease B is an important virulence factor that is required for Helicobacter pylori to colonise the gastric mucosa. Mouse monoclonal antibodies (mAbs) that inhibit urease B enzymatic activity will be useful as vaccines for the prevention and treatment of H. pylori infection. Here, we produced murine mAbs agai...

journal_title：BMC biotechnology

authors： Li Y,Ning Y,Wang Y,Peng D,Jiang Y,Zhang L,Long M,Luo J,Li M

更新日期：2010-11-30 00:00:00

abstract：BACKGROUND:The CRISPR/Cas9 system is increasingly used for gene inactivation in mouse zygotes, but homology-directed mutagenesis and use of inbred embryos are less established. In particular, Rosa26 knock-in alleles for the insertion of transgenes in a genomic 'safe harbor' site, have not been produced. Here we applied...

journal_title：BMC biotechnology

authors： Chu VT,Weber T,Graf R,Sommermann T,Petsch K,Sack U,Volchkov P,Rajewsky K,Kühn R

更新日期：2016-01-16 00:00:00

abstract：BACKGROUND:Smoking and tobacco use continue to be the largest preventable causes of death globally. A novel therapeutic approach has recently been proposed: administration of an enzyme that degrades nicotine, the main addictive component of tobacco, minimizing brain exposure and reducing its reinforcing effects. Pre-cl...

journal_title：BMC biotechnology

authors： Thisted T,Biesova Z,Walmacq C,Stone E,Rodnick-Smith M,Ahmed SS,Horrigan SK,Van Engelen B,Reed C,Kalnik MW

更新日期：2019-08-02 00:00:00

abstract：BACKGROUND:More than a dozen of fungal immunomodulatory proteins (FIPs) have been identified to date, most of which are from Ganoderma species. However, little is known about the similarities and differences between different Ganoderma FIPs' bioactivities. In the current study, two FIP genes termed FIP-gap1 and FIP-gap...

journal_title：BMC biotechnology

authors： Qu ZW,Zhou SY,Guan SX,Gao R,Duan ZW,Zhang X,Sun WY,Fan WL,Chen SS,Chen LJ,Lin JW,Ruan YY

更新日期：2018-12-14 00:00:00

abstract：BACKGROUND:One of the most commonly used vectors for gene therapy is the adenoviral vector; its ability to tightly regulate transgene expression is critical for optimizing therapeutic outcomes. The tetracycline-regulated system (especially the Tet-On system) for gene expression is one of the most valuable tools for con...

journal_title：BMC biotechnology

authors： Chen H,Wang D,Xia R,Mao Q,Xia H

更新日期：2015-02-13 00:00:00

abstract：BACKGROUND:Xylanase can replace chemical additives to improve the volume and sensory properties of bread in the baking. Suitable baking xylanase with improved yield will promote the application of xylanase in baking industry. The xylanase XYNZG from the Plectosphaerella cucumerina has been previously characterized by h...

journal_title：BMC biotechnology

authors： Zhan FX,Wang QH,Jiang SJ,Zhou YL,Zhang GM,Ma YH

更新日期：2014-12-16 00:00:00

abstract：BACKGROUND:L-ornithine is effective in the treatment of liver diseases and helps strengthen the heart. The commercial applications mean that efficient biotechnological production of L-ornithine has become increasingly necessary. Adaptive evolution strategies have been proven a feasible and efficient technique to achiev...

journal_title：BMC biotechnology

authors： Jiang LY,Chen SG,Zhang YY,Liu JZ

更新日期：2013-06-01 00:00:00

abstract：BACKGROUND:Heat-stable antifungal factor (HSAF) is a newly identified broad-spectrum antifungal antibiotic from the biocontrol agent Lysobacter enzymogenes and is regarded as a potential biological pesticide, due to its novel mode of action. However, the production level of HSAF is quite low, and little research has re...

journal_title：BMC biotechnology

authors： Tang B,Sun C,Zhao Y,Xu H,Xu G,Liu F

更新日期：2018-10-24 00:00:00

abstract：BACKGROUND:The green fluorescent protein (GFP) has been regarded as a valuable tool and widely applied as a biomarker in medical applications and diagnostics. A cost-efficient upstream expression system and an inexpensive downstream purification process will meet the demands of the GFP protein with high-purity. RESULT...

journal_title：BMC biotechnology

authors： Dong J,Ding X,Wang S

更新日期：2019-12-09 00:00:00

abstract：BACKGROUND:Ligand-targeted approaches have proven successful in improving the therapeutic index of a number of drugs. We hypothesized that the specific targeting of TNF-alpha antagonists to inflamed tissues could increase drug efficacy and reduce side effects. RESULTS:Using uteroglobin (UG), a potent anti-inflammatory...

journal_title：BMC biotechnology

authors： Ventura E,Balza E,Borsi L,Tutolo G,Carnemolla B,Castellani P,Zardi L

更新日期：2011-11-10 00:00:00

abstract：BACKGROUND:Porcine reproductive and respiratory syndrome virus (PRRSV) causes major economic losses in the pig industry worldwide. In vivo, the virus infects a subpopulation of tissue macrophages. In vitro, PRRSV only replicates in primary pig macrophages and African green monkey kidney derived cells, such as Marc-145....

journal_title：BMC biotechnology

authors： Delrue I,Van Gorp H,Van Doorsselaere J,Delputte PL,Nauwynck HJ

更新日期：2010-06-29 00:00:00

abstract：BACKGROUND:The ability of cytosine deaminase (CD) to convert the antifungal agent 5-fluorocytosine (5-FC) into one of the most potent and largely used anticancer compound such as 5-fluorouracil (5-FU) raised considerable interest in this enzyme to model gene or antibody - directed enzyme-prodrug therapy (GDEPT/ADEPT) a...

journal_title：BMC biotechnology

authors： Mallano A,Zamboni S,Carpinelli G,Santoro F,Flego M,Ascione A,Gellini M,Tombesi M,Podo F,Cianfriglia M

更新日期：2008-09-10 00:00:00

abstract：BACKGROUND:Clostridial co-culture containing cellulolytic and solventogenic species is a potential consolidated bioprocessing (CBP) approach for producing biochemicals and biofuels from cellulosic biomass. It has been demonstrated that the rate of cellulose utilization in the co-culture of Clostridium acetobutylicum an...

journal_title：BMC biotechnology

authors： Salimi F,Mahadevan R

更新日期：2013-11-04 00:00:00

abstract：BACKGROUND:Prostate cancer is the most-diagnosed non-skin cancer among males in the US, and the second leading cause of cancer-related death. Current methods of treatment and diagnosis are not specific for the disease. This work identified an antibody fragment that binds selectively to a molecule on the surface of andr...

journal_title：BMC biotechnology

authors： Williams RM,Hajiran CJ,Nayeem S,Sooter LJ

更新日期：2014-09-03 00:00:00

abstract：BACKGROUND:Bacterial degradation/transformation of steroids is widely investigated to create biotechnologically relevant strains for industrial application. The strain of Nocardioides simplex VKM Ac-2033D is well known mainly for its superior 3-ketosteroid Δ1-dehydrogenase activity towards various 3-oxosteroids and oth...

journal_title：BMC biotechnology

authors： Shtratnikova VY,Sсhelkunov MI,Fokina VV,Bragin EY,Shutov AA,Donova MV

更新日期：2021-01-13 00:00:00

abstract：BACKGROUND:Understanding and manipulating gene function in physiological conditions is a major objective for both fundamental and applied research. In contrast to other experimental settings, which use either purely genetic or gene delivery (viral or non-viral) strategies, we report here a strategy based on direct prot...

journal_title：BMC biotechnology

authors： Gitton Y,Tibaldi L,Dupont E,Levi G,Joliot A

更新日期：2009-04-24 00:00:00

abstract：BACKGROUND:Dissolved oxygen tension (DOT) is hardly constant and homogenously distributed in a bioreactor, which can have a negative impact in the metabolism and product synthesis. However, the effects of DOT on plasmid DNA (pDNA) production and quality have not been thoroughly investigated. In the present study, the e...

journal_title：BMC biotechnology

authors： Jaén KE,Sigala JC,Olivares-Hernández R,Niehaus K,Lara AR

更新日期：2017-07-04 00:00:00

abstract：BACKGROUND:Plants may represent excellent alternatives to classical heterologous protein expression systems, especially for the production of biopharmaceuticals and vaccine components. Modern vaccines are becoming increasingly complex, with the incorporation of multiple antigens. Approaches towards developing an HIV va...

journal_title：BMC biotechnology

authors： Marusic C,Nuttall J,Buriani G,Lico C,Lombardi R,Baschieri S,Benvenuto E,Frigerio L

更新日期：2007-02-26 00:00:00

abstract：BACKGROUND:Whole genome amplification (WGA) promises to eliminate practical molecular genetic analysis limitations associated with genomic DNA (gDNA) quantity. We evaluated the performance of multiple displacement amplification (MDA) WGA using gDNA extracted from lymphoblastoid cell lines (N = 27) with a range of start...

journal_title：BMC biotechnology

authors： Bergen AW,Qi Y,Haque KA,Welch RA,Chanock SJ

更新日期：2005-09-16 00:00:00

abstract：BACKGROUND:Each year, influenza is responsible for hundreds of thousand cases of illness and deaths worldwide. Due to the virus' fast mutation rate, the World Health Organization (WHO) is constantly on alert to rapidly respond to emerging pandemic strains. Although anti-viral therapies exist, the most proficient way to...

journal_title：BMC biotechnology

authors： Thompson CM,Petiot E,Mullick A,Aucoin MG,Henry O,Kamen AA

更新日期：2015-05-16 00:00:00

abstract：BACKGROUND:Acetyl-xylan esterase (AXE, EC 3.1.1.72) hydrolyses acetate group from the linear chain of xylopyranose residues bound by β-1,4-linkage. The enzyme finds commercial applications in bio-bleaching of wood pulp, treating animal feed to increase digestibility, processing food to increase clarification and conver...

journal_title：BMC biotechnology

authors： Atta S,Ali S,Akhtar MN,Haq I

更新日期：2011-05-16 00:00:00

abstract：BACKGROUND:Spinal cord injury (SCI) remains a huge medical problem nowadays as there is no hospital providing the versatile strategies for repairing central nervous system and restoring function. Herein, we focused on PC-12 cells as an important research tool and studied the potential role of resveratrol (RSV) in infla...

journal_title：BMC biotechnology

authors： Zhang G,Liu Y,Xu L,Sha C,Zhang H,Xu W

更新日期：2019-01-28 00:00:00

abstract：BACKGROUND:The detection of unknown mutations is important in research and medicine. For this purpose, a mismatch-specific endonuclease CEL I from celery has been established as a useful tool in high throughput projects. Previously, CEL I-like activities were described only in a variety of plants and could not be expre...

journal_title：BMC biotechnology

authors： Pimkin M,Caretti E,Canutescu A,Yeung JB,Cohn H,Chen Y,Oleykowski C,Bellacosa A,Yeung AT

更新日期：2007-06-01 00:00:00

abstract：BACKGROUND:Polymerase chain reaction (PCR) is one of the most important developments in modern biotechnology. However, PCR is known to introduce biases, especially during multiplex reactions. Recent studies have implicated the DNA polymerase as the primary source of bias, particularly initiation of polymerization on th...

journal_title：BMC biotechnology

authors： Pan W,Byrne-Steele M,Wang C,Lu S,Clemmons S,Zahorchak RJ,Han J

更新日期：2014-01-30 00:00:00