Abstract：
BACKGROUND:Rapid, robust and reversible induction of transgene expression would significantly facilitate cancer gene therapy as well as allow the in vivo functional study of newly discovered genes in tumor formation and progression. The popularity of the ecdysone inducible gene switch system has led us to investigate whether such a system can successfully regulate gene expression in a syngeneic tumor system in vivo. RESULTS:MBT-2 and Panc02 carcinoma cells were transfected with components of a modification of the ecdysone switch system driving firefly luciferase (F-Luc). In vitro luciferase expression +/- ecdysone analog GS-E indicated a robust induction with minimal baseline activity and complete decay after 24 hours without drug. In vitro selection of MBT-2 transfected cell clones which had complete absence of F-Luc expression in the absence of stimulation but which expressed this gene at high levels in response to GS-E were chosen for in vivo evaluation. Tumors from engineered MBT-2 cells were grown to 5 mm in diameter prior to GS-E administration, animals euthanized and tumors removed at 6, 12 and 24 hours after GS-E administration and assayed for F-Luc activity. GS-E resulted in a maximal induction of F-Luc activity at 6 hours in tumor tissue with almost complete reversion to control levels by 12 hours. CONCLUSIONS:This study is the first demonstration that robust and reversible transgene expression in tumors is feasible using the ecdysone system, allowing future rapid in vivo functional characterization of gene function or gene therapy applications.
journal_name
BMC Biotechnoljournal_title
BMC biotechnologyauthors
Karns LR,Kisielewski A,Gulding KM,Seraj JM,Theodorescu Ddoi
10.1186/1472-6750-1-11keywords:
subject
Has Abstractpub_date
2001-01-01 00:00:00pages
11issn
1472-6750journal_volume
1pub_type
杂志文章abstract：BACKGROUND:The extracellular domain of matrix protein 2 (M2e) of influenza A virus is a promising target for the development of a universal vaccine against influenza because M2e sequences are highly conserved among human influenza A strains. However, native M2e is poorly immunogenic, but its immunogenicity can be incre...
journal_title：BMC biotechnology
pub_type： 杂志文章
doi：10.1186/s12896-015-0164-6
更新日期：2015-05-29 00:00:00
abstract：BACKGROUND:Bacterial degradation/transformation of steroids is widely investigated to create biotechnologically relevant strains for industrial application. The strain of Nocardioides simplex VKM Ac-2033D is well known mainly for its superior 3-ketosteroid Δ1-dehydrogenase activity towards various 3-oxosteroids and oth...
journal_title：BMC biotechnology
pub_type： 杂志文章
doi：10.1186/s12896-021-00668-9
更新日期：2021-01-13 00:00:00
abstract：BACKGROUND:The CRISPR/Cas9 genome editing system has greatly facilitated and expanded our capacity to engineer mammalian genomes, including targeted gene knock-outs. However, the phenotyping of the knock-out effect requires a high DNA editing efficiency. RESULTS:Here, we report a user-friendly strategy based on the ex...
journal_title：BMC biotechnology
pub_type： 杂志文章
doi：10.1186/s12896-016-0250-4
更新日期：2016-02-16 00:00:00
abstract：BACKGROUND:In the last three decades, the Baculovirus expression vector system (BEV) has evolved to one of the most widely used eukaryotic systems for heterologous protein expression including approved vaccines and therapies. Despite the significant improvements introduced during the past years, the BEV system still ha...
journal_title：BMC biotechnology
pub_type： 杂志文章
doi：10.1186/s12896-017-0400-3
更新日期：2017-11-16 00:00:00
abstract：BACKGROUND:Serine hydroxymethyltransferase (SHMT) is the key enzyme in L-serine enzymatic production, suggesting the importance of obtaining a SHMT with high activity. RESULTS:Here, a novel SHMT gene, glyA, was obtained through degenerate oligonucleotide-primed PCR and encoded a novel SHMT with 54.3% similarity to the...
journal_title：BMC biotechnology
pub_type： 杂志文章
doi：10.1186/s12896-014-0093-9
更新日期：2014-11-14 00:00:00
abstract：BACKGROUND:Multi-copy gene integration into microbial genomes is a conventional tool for obtaining improved gene expression. For Penicillium chrysogenum, the fungal producer of the beta-lactam antibiotic penicillin, many production strains carry multiple copies of the penicillin biosynthesis gene cluster. This discover...
journal_title：BMC biotechnology
pub_type： 杂志文章
doi：10.1186/s12896-017-0335-8
更新日期：2017-02-16 00:00:00
abstract：BACKGROUND:The green fluorescent protein (GFP) has been regarded as a valuable tool and widely applied as a biomarker in medical applications and diagnostics. A cost-efficient upstream expression system and an inexpensive downstream purification process will meet the demands of the GFP protein with high-purity. RESULT...
journal_title：BMC biotechnology
pub_type： 杂志文章
doi：10.1186/s12896-019-0590-y
更新日期：2019-12-09 00:00:00
abstract：BACKGROUND:Xenoantigens are a major source of concern with regard to the success of interspecific xenografts. GGTA1 encodes α1,3-galactosyltransferase, which is essential for the biosynthesis of galactosyl-alpha 1,3-galactose, the major xenoantigen causing hyperacute rejection. GGTA1-modified pigs, therefore, are promi...
journal_title：BMC biotechnology
pub_type： 杂志文章
doi：10.1186/s12896-020-00638-7
更新日期：2020-08-18 00:00:00
abstract：BACKGROUND:Dengue is a public health problem of global significance for which there is neither an effective antiviral therapy nor a preventive vaccine. It is a mosquito-borne viral disease, caused by dengue (DEN) viruses, which are members of the Flaviviridae family. There are four closely related serotypes, DEN-1, DEN...
journal_title：BMC biotechnology
pub_type： 杂志文章
doi：10.1186/1472-6750-7-10
更新日期：2007-02-15 00:00:00
abstract：BACKGROUND:Vascular endothelial growth factor (VEGF) is a key angiogenic factors. It plays an important role in both physiologic and pathologic angiogenesis and increases permeability across the vessels. Using antibody phage display technology, we obtained a novel anti-VEGFA IgG, named as FD006. In this study, the phar...
journal_title：BMC biotechnology
pub_type： 杂志文章
doi：10.1186/1472-6750-14-17
更新日期：2014-02-27 00:00:00
abstract：BACKGROUND:Splicing of DNA molecules is an important task in molecular biology that facilitates cloning, mutagenesis and creation of chimeric genes. Mutagenesis and DNA splicing techniques exist, some requiring restriction enzymes, and others utilize staggered reannealing approaches. RESULTS:A method for DNA splicing ...
journal_title：BMC biotechnology
pub_type： 杂志文章
doi：10.1186/1472-6750-5-23
更新日期：2005-09-01 00:00:00
abstract：BACKGROUND:Methylated CpG dinucleotides in promoters are associated with the loss of gene expression in recombinant Chinese hamster ovary (CHO) cells during large-scale commercial manufacturing. We evaluated a promoter devoid of CpG dinucleotides, CpGfree, in parallel with a similar CpG containing promoter, CpGrich, fo...
journal_title：BMC biotechnology
pub_type： 杂志文章
doi：10.1186/s12896-016-0300-y
更新日期：2016-10-18 00:00:00
abstract：BACKGROUND:Carboxyl-terminal protease (CtpA) plays essential functions in posttranslational protein processing in prokaryotic and eukaryotic cells. To date, only a few bacterial ctpA genes have been characterized. Here we cloned and characterized a novel CtpA. The encoding gene, ctpAp (ctpA of Paenibacillus lautus), wa...
journal_title：BMC biotechnology
pub_type： 杂志文章
doi：10.1186/1472-6750-13-89
更新日期：2013-10-25 00:00:00
abstract：BACKGROUND:Thermally stable α-L-rhamnosidase with cleaving terminal α-L-rhamnose activity has great potential in industrial application. Therefore, it is necessary to find a proper method to improve the thermal stability of α-L-rhamnosidase. RESULTS:In this study, addition of sorbitol has been found to increase the th...
journal_title：BMC biotechnology
pub_type： 杂志文章
doi：10.1186/s12896-017-0342-9
更新日期：2017-02-27 00:00:00
abstract：BACKGROUND:The use of small interfering RNA (siRNA) molecules in animals to achieve double-stranded RNA-mediated interference (RNAi) has recently emerged as a powerful method of sequence-specific gene knockdown. As DNA-based expression of short hairpin RNA (shRNA) for RNAi may offer some advantages over chemical and in...
journal_title：BMC biotechnology
pub_type： 杂志文章
doi：10.1186/1472-6750-5-13
更新日期：2005-05-11 00:00:00
abstract：BACKGROUND:Social amoeba, Dictyostelium discoideum, is a well-established model organism for studying cellular physiology and developmental pattern formation. Its haploid genome facilitates functional analysis of genes by a single round of mutagenesis including targeted disruption. Although the efficient generation of ...
journal_title：BMC biotechnology
pub_type： 杂志文章
doi：10.1186/s12896-016-0267-8
更新日期：2016-04-14 00:00:00
abstract：BACKGROUND:Oleaginous yeast species are an alternative for the production of lipids or triacylglycerides (TAGs). These yeasts are usually non-pathogenic and able to store TAGs ranging from 20 % to 70 % of their cell mass depending on culture conditions. TAGs originating from oleaginous yeasts can be used as the so-call...
journal_title：BMC biotechnology
pub_type： 杂志文章
doi：10.1186/s12896-016-0276-7
更新日期：2016-05-27 00:00:00
abstract：BACKGROUND:The cattle ticks, Boophilus spp., affect cattle production in tropical and subtropical regions of the world. Tick vaccines constitute a cost-effective and environmentally friendly alternative to tick control. The recombinant B. microplus Bm86 protective antigen has been shown to protect cattle against tick i...
journal_title：BMC biotechnology
pub_type： 杂志文章
doi：10.1186/1472-6750-9-29
更新日期：2009-03-31 00:00:00
abstract：BACKGROUND:Expression vector engineering technology is one of the most convenient and timely method for cell line development to meet the rising demand of novel production cell line with high productivity. Destabilization of dihydrofolate reductase (dhfr) selection marker by addition of AU-rich elements and murine orni...
journal_title：BMC biotechnology
pub_type： 杂志文章
doi：10.1186/s12896-015-0145-9
更新日期：2015-06-02 00:00:00
abstract：BACKGROUND:At first glance, there appears to be a high degree of similarity between the metabolism of yeast (the Crabtree effect) and human cancer cells (the Warburg effect). At the root of both effects is accelerated metabolic flow through glycolysis which leads to overflows of ethanol and lactic acid, respectively. I...
journal_title：BMC biotechnology
pub_type： 杂志文章
doi：10.1186/s12896-017-0362-5
更新日期：2017-05-08 00:00:00
abstract：BACKGROUND:Advances in optical imaging modalities and the continued evolution of genetically-encoded fluorescent proteins are coming together to facilitate the study of cell behavior at high resolution in living organisms. As a result, imaging using autofluorescent protein reporters is gaining popularity in mouse trans...
journal_title：BMC biotechnology
pub_type： 杂志文章
doi：10.1186/1472-6750-4-33
更新日期：2004-12-24 00:00:00
abstract：BACKGROUND:Equine chorionic gonadotropin (eCG) induces super-ovulation in laboratory animals. Notwithstanding its extensive usage, limited information is available regarding the differences between the in vivo effects of natural eCG (N-eCG) and recombinant eCG (R-eCG). This study aimed to investigate the gene expressio...
journal_title：BMC biotechnology
pub_type： 杂志文章
doi：10.1186/s12896-020-00653-8
更新日期：2020-11-11 00:00:00
abstract：BACKGROUND:There are significant differences in the culture conditions between small-scale screenings and large-scale fermentation processes. Production processes are usually conducted in fed-batch cultivation mode with active pH-monitoring and control. In contrast, screening experiments in shake flasks are usually con...
journal_title：BMC biotechnology
pub_type： 杂志文章
doi：10.1186/1472-6750-11-25
更新日期：2011-03-23 00:00:00
abstract：BACKGROUND:Polymerase chain reaction (PCR) is one of the most important developments in modern biotechnology. However, PCR is known to introduce biases, especially during multiplex reactions. Recent studies have implicated the DNA polymerase as the primary source of bias, particularly initiation of polymerization on th...
journal_title：BMC biotechnology
pub_type： 杂志文章
doi：10.1186/1472-6750-14-10
更新日期：2014-01-30 00:00:00
abstract：BACKGROUND:With the increasing number of GMOs on the global market the maintenance of European GMO regulations is becoming more complex. For the analysis of a single food or feed sample it is necessary to assess the sample for the presence of many GMO-targets simultaneously at a sensitive level. Several methods have be...
journal_title：BMC biotechnology
pub_type： 杂志文章
doi：10.1186/1472-6750-12-4
更新日期：2012-01-19 00:00:00
abstract：BACKGROUND:The therapeutic use of α-amidated peptides (e.g. calcitonin, glucagon-like peptide) has increased dramatically, but there are major impediments to wider use of such peptides. Larger peptides are expensive to synthesize, and short plasma half-lives frequently limit the clinical circumstances in which the pept...
journal_title：BMC biotechnology
pub_type： 杂志文章
doi：10.1186/s12896-015-0173-5
更新日期：2015-06-27 00:00:00
abstract：BACKGROUND:The ubiquitin protein is present in all eukaryotic cells and promoters from ubiquitin genes are good candidates to regulate the constitutive expression of transgenes in plants. Therefore, two switchgrass (Panicum virgatum L.) ubiquitin genes (PvUbi1 and PvUbi2) were cloned and characterized. Reporter constru...
journal_title：BMC biotechnology
pub_type： 杂志文章
doi：10.1186/1472-6750-11-74
更新日期：2011-07-11 00:00:00
abstract：BACKGROUND:There is an increasing need to understand cell-cell interactions for cell and tissue engineering purposes, such as optimizing cell sheet constructs, as well as for examining adhesion defect diseases. For cell-sheet engineering, one major obstacle to sheet function is that cell sheets in suspension are fragil...
journal_title：BMC biotechnology
pub_type： 杂志文章
doi：10.1186/1472-6750-13-17
更新日期：2013-02-26 00:00:00
abstract：BACKGROUND:Taxol is an effective antineoplastic agent, originally extracted from the bark of Taxus brevifolia with a low yield. Many attempts have been made to produce Taxol by chemical synthesis, semi-synthesis and plant tissue cultures. However, to date, the availability of this compound is not sufficient to satisfy ...
journal_title：BMC biotechnology
pub_type： 杂志文章
doi：10.1186/1472-6750-6-45
更新日期：2006-12-06 00:00:00
abstract：BACKGROUND:Spinal cord injury (SCI) remains a huge medical problem nowadays as there is no hospital providing the versatile strategies for repairing central nervous system and restoring function. Herein, we focused on PC-12 cells as an important research tool and studied the potential role of resveratrol (RSV) in infla...
journal_title：BMC biotechnology
pub_type： 杂志文章
doi：10.1186/s12896-019-0502-1
更新日期：2019-01-28 00:00:00
