Membrane-bound and soluble forms of an NMDA receptor extracellular domain retain epitopes targeted in auto-immune encephalitis.

abstract：BACKGROUND:In spite of a great number of monomeric fluorescent proteins developed in the recent years, the reported fluorescent protein-based FRET pairs are still characterized by a number of disadvantageous features, complicating their use as reporters in cell biology and for high-throughput cell-based screenings. RE...

journal_title：BMC biotechnology

authors： Shcherbo D,Souslova EA,Goedhart J,Chepurnykh TV,Gaintzeva A,Shemiakina II,Gadella TW,Lukyanov S,Chudakov DM

更新日期：2009-03-25 00:00:00

abstract：BACKGROUND:The cattle ticks, Boophilus spp., affect cattle production in tropical and subtropical regions of the world. Tick vaccines constitute a cost-effective and environmentally friendly alternative to tick control. The recombinant B. microplus Bm86 protective antigen has been shown to protect cattle against tick i...

journal_title：BMC biotechnology

authors： Canales M,Almazán C,Naranjo V,Jongejan F,de la Fuente J

更新日期：2009-03-31 00:00:00

abstract：BACKGROUND:Mesenchymal stem cells (MSCs) are an attractive source of adult stem cells for therapeutic application in clinical study. Genetic modification of MSCs with beneficial genes makes them more effective for therapeutic use. However, it is difficult to transduce genes into MSCs by common transfection methods, esp...

journal_title：BMC biotechnology

authors： Lim JY,Park SH,Jeong CH,Oh JH,Kim SM,Ryu CH,Park SA,Ahn JG,Oh W,Jeun SS,Chang JW

更新日期：2010-05-13 00:00:00

abstract：BACKGROUND:Ultrasound and microbubbles (USMB) have been shown to enhance the intracellular uptake of molecules, generally thought to occur as a result of sonoporation. The underlying mechanism associated with USMB-enhanced intracellular uptake such as membrane disruption and endocytosis may also be associated with USMB...

journal_title：BMC biotechnology

authors： Hussein F,Antonescu C,Karshafian R

更新日期：2017-05-18 00:00:00

abstract：BACKGROUND:The recent H1N1 influenza pandemic illustrated the shortcomings of the vaccine manufacturing process. The A/California/07/2009 H1N1 pandemic influenza vaccine or A(H1N1)pdm09 was available late and in short supply as a result of delays in production caused by low yields and poor antigen stability. Recombinan...

journal_title：BMC biotechnology

authors： Feshchenko E,Rhodes DG,Felberbaum R,McPherson C,Rininger JA,Post P,Cox MM

更新日期：2012-10-30 00:00:00

abstract：BACKGROUND:Iron is an essential element in several pathways of microbial metabolism, and therefore low iron toxicity is expected on the usage of Fe nanoparticles (NPs). This study aims to determine the effect of Fe NPs on biosurfactant production by marine actinobacterium Nocardiopsis sp. MSA13A under solid state cultu...

journal_title：BMC biotechnology

authors： Kiran GS,Nishanth LA,Priyadharshini S,Anitha K,Selvin J

更新日期：2014-05-21 00:00:00

abstract：BACKGROUND:Clostridial co-culture containing cellulolytic and solventogenic species is a potential consolidated bioprocessing (CBP) approach for producing biochemicals and biofuels from cellulosic biomass. It has been demonstrated that the rate of cellulose utilization in the co-culture of Clostridium acetobutylicum an...

journal_title：BMC biotechnology

authors： Salimi F,Mahadevan R

更新日期：2013-11-04 00:00:00

abstract：BACKGROUND:Nitrilases attract increasing attention due to their utility in the mild hydrolysis of nitriles. According to activity and gene screening, filamentous fungi are a rich source of nitrilases distinct in evolution from their widely examined bacterial counterparts. However, fungal nitrilases have been less explo...

journal_title：BMC biotechnology

authors： Kaplan O,Bezouška K,Plíhal O,Ettrich R,Kulik N,Vaněk O,Kavan D,Benada O,Malandra A,Sveda O,Veselá AB,Rinágelová A,Slámová K,Cantarella M,Felsberg J,Dušková J,Dohnálek J,Kotik M,Křen V,Martínková L

更新日期：2011-01-06 00:00:00

abstract：BACKGROUND:There is an imperative necessity for alternative sources of energy able to reduce the world dependence of fossil oil. One of the most successful options is ethanol obtained mainly from sugarcane and corn fermentation. The foremost residue from sugarcane industry is the bagasse, a rich lignocellulosic raw mat...

journal_title：BMC biotechnology

authors： Robl D,Delabona Pda S,Mergel CM,Rojas JD,Costa Pdos S,Pimentel IC,Vicente VA,da Cruz Pradella JG,Padilla G

更新日期：2013-10-31 00:00:00

abstract：BACKGROUND:There are significant differences in the culture conditions between small-scale screenings and large-scale fermentation processes. Production processes are usually conducted in fed-batch cultivation mode with active pH-monitoring and control. In contrast, screening experiments in shake flasks are usually con...

journal_title：BMC biotechnology

authors： Scheidle M,Dittrich B,Klinger J,Ikeda H,Klee D,Büchs J

更新日期：2011-03-23 00:00:00

abstract：BACKGROUND:Human TGF-β3 has been used in many studies to induce genes coding for typical cartilage matrix components and accelerate chondrogenic differentiation, making it the standard constituent in most cultivation media used for the assessment of chondrogenesis associated with various stem cell types on carrier matr...

journal_title：BMC biotechnology

authors： Huang Y,Seitz D,Chevalier Y,Müller PE,Jansson V,Klar RM

更新日期：2020-08-27 00:00:00

abstract：BACKGROUND:β-Fructofuranosidases (or invertases) catalyse the commercially-important biotransformation of sucrose into short-chain fructooligosaccharides with wide-scale application as a prebiotic in the functional foods and pharmaceutical industries. RESULTS:We identified a β-fructofuranosidase gene (CmINV) from a Ce...

journal_title：BMC biotechnology

authors： van Wyk N,Trollope KM,Steenkamp ET,Wingfield BD,Volschenk H

更新日期：2013-11-14 00:00:00

abstract：BACKGROUND:Circular forms of viral genomic DNA are generated during infection of cells with retroviruses like HIV-1. Such circles are unable to replicate and are eventually lost as a result of cell division, lending support to the prevalent notion that episomal retroviral DNA forms are dead-end products of reverse tran...

journal_title：BMC biotechnology

authors： Moldt B,Staunstrup NH,Jakobsen M,Yáñez-Muñoz RJ,Mikkelsen JG

更新日期：2008-08-09 00:00:00

abstract：BACKGROUND:Size exclusion chromatography is the method of choice for separating free from liposome-encapsulated molecules. However, if the column is not presaturated with lipids this type of chromatography causes a significant loss of lipid material. To date, the mechanism of lipid retention is poorly understood. It ha...

journal_title：BMC biotechnology

authors： Ruysschaert T,Marque A,Duteyrat JL,Lesieur S,Winterhalter M,Fournier D

更新日期：2005-05-10 00:00:00

abstract：BACKGROUND:A method for inhibiting the expression of particular genes using external guide sequences (EGSs) has been developed in bacteria, mammalian cells and maize cells. RESULTS:To examine whether EGS technology can be used to down-regulate gene expression in Caenorhabditis elegans (C. elegans), we generated EGS-Ng...

journal_title：BMC biotechnology

authors： Yan Q,Zhao R,Zheng W,Yin C,Zhang B,Ma W

更新日期：2009-05-20 00:00:00

abstract：BACKGROUND:More than a dozen of fungal immunomodulatory proteins (FIPs) have been identified to date, most of which are from Ganoderma species. However, little is known about the similarities and differences between different Ganoderma FIPs' bioactivities. In the current study, two FIP genes termed FIP-gap1 and FIP-gap...

journal_title：BMC biotechnology

authors： Qu ZW,Zhou SY,Guan SX,Gao R,Duan ZW,Zhang X,Sun WY,Fan WL,Chen SS,Chen LJ,Lin JW,Ruan YY

更新日期：2018-12-14 00:00:00

abstract：BACKGROUND:Insect resistance in crops represents a main challenge for agriculture. Transgenic approaches based on proteins displaying insect resistance properties are widely used as efficient breeding strategies. To extend the spectrum of targeted pathogens and overtake the development of resistance, molecular evolutio...

journal_title：BMC biotechnology

authors： Prado GS,Bamogo PKA,de Abreu JAC,Gillet FX,Dos Santos VO,Silva MCM,Brizard JP,Bemquerer MP,Bangratz M,Brugidou C,Sérémé D,Grossi-de-Sa MF,Lacombe S

更新日期：2019-03-08 00:00:00

abstract：BACKGROUND:Taxol is an effective antineoplastic agent, originally extracted from the bark of Taxus brevifolia with a low yield. Many attempts have been made to produce Taxol by chemical synthesis, semi-synthesis and plant tissue cultures. However, to date, the availability of this compound is not sufficient to satisfy ...

journal_title：BMC biotechnology

authors： Bestoso F,Ottaggio L,Armirotti A,Balbi A,Damonte G,Degan P,Mazzei M,Cavalli F,Ledda B,Miele M

更新日期：2006-12-06 00:00:00

abstract：BACKGROUND:Each year, influenza is responsible for hundreds of thousand cases of illness and deaths worldwide. Due to the virus' fast mutation rate, the World Health Organization (WHO) is constantly on alert to rapidly respond to emerging pandemic strains. Although anti-viral therapies exist, the most proficient way to...

journal_title：BMC biotechnology

authors： Thompson CM,Petiot E,Mullick A,Aucoin MG,Henry O,Kamen AA

更新日期：2015-05-16 00:00:00

abstract：BACKGROUND:Construction of high quality cDNA libraries from the usually low amounts of eukaryotic mRNA extracted from environmental samples is essential in functional metatranscriptomics for the selection of functional, full-length genes encoding proteins of interest. Many of the inserts in libraries constructed by sta...

journal_title：BMC biotechnology

authors： Yadav RK,Barbi F,Ziller A,Luis P,Marmeisse R,Reddy MS,Fraissinet-Tachet L

更新日期：2014-09-03 00:00:00

abstract：BACKGROUND:The isothermal amplification of RNA in vitro has been used for the study of in vitro evolution of RNA. Although Qβ replicase has been traditionally used as an enzyme for this purpose, we planned to use norovirus replicase (NV3D(pol)) due to its structural simplicity in the scope of in vitro autonomous evolut...

journal_title：BMC biotechnology

authors： Arai H,Nishigaki K,Nemoto N,Suzuki M,Husimi Y

更新日期：2013-10-09 00:00:00

abstract：BACKGROUND:During a global influenza pandemic, the vaccine requirements of developing countries can surpass their supply capabilities, if these exist at all, compelling them to rely on developed countries for stocks that may not be available in time. There is thus a need for developing countries in general to produce t...

journal_title：BMC biotechnology

authors： Mortimer E,Maclean JM,Mbewana S,Buys A,Williamson AL,Hitzeroth II,Rybicki EP

更新日期：2012-04-26 00:00:00

abstract：BACKGROUND:The identification of known mutations in a cell population is important for clinical applications and basic cancer research. In this work an immobilized form of the polymerase chain reaction, referred to as polony technology, was used to detect mutations as well as gene deletions, resulting in loss of hetero...

journal_title：BMC biotechnology

authors： Butz J,Wickstrom E,Edwards J

更新日期：2003-07-23 00:00:00

abstract：BACKGROUND:Understanding and manipulating gene function in physiological conditions is a major objective for both fundamental and applied research. In contrast to other experimental settings, which use either purely genetic or gene delivery (viral or non-viral) strategies, we report here a strategy based on direct prot...

journal_title：BMC biotechnology

authors： Gitton Y,Tibaldi L,Dupont E,Levi G,Joliot A

更新日期：2009-04-24 00:00:00

abstract：BACKGROUND:Oleaginous yeast species are an alternative for the production of lipids or triacylglycerides (TAGs). These yeasts are usually non-pathogenic and able to store TAGs ranging from 20 % to 70 % of their cell mass depending on culture conditions. TAGs originating from oleaginous yeasts can be used as the so-call...

journal_title：BMC biotechnology

authors： Lamers D,van Biezen N,Martens D,Peters L,van de Zilver E,Jacobs-van Dreumel N,Wijffels RH,Lokman C

更新日期：2016-05-27 00:00:00

abstract：BACKGROUND:Urease B is an important virulence factor that is required for Helicobacter pylori to colonise the gastric mucosa. Mouse monoclonal antibodies (mAbs) that inhibit urease B enzymatic activity will be useful as vaccines for the prevention and treatment of H. pylori infection. Here, we produced murine mAbs agai...

journal_title：BMC biotechnology

authors： Li Y,Ning Y,Wang Y,Peng D,Jiang Y,Zhang L,Long M,Luo J,Li M

更新日期：2010-11-30 00:00:00

abstract：BACKGROUND:The CRISPR-Cas9 system is a powerful and versatile tool for crop genome editing. However, achieving highly efficient and specific editing in polyploid species can be a challenge. The efficiency and specificity of the CRISPR-Cas9 system depends critically on the gRNA used. Here, we assessed the activities and...

journal_title：BMC biotechnology

authors： Arndell T,Sharma N,Langridge P,Baumann U,Watson-Haigh NS,Whitford R

更新日期：2019-10-30 00:00:00

abstract：BACKGROUND:In the previous study, the cellulolytic Escherichia coli ZH-4 isolated from bovine rumen was found to show extracellular cellulase activity and could degrade cellulose in the culture. The goal of this work was to identify and characterize the secreted cellulase of E. coli ZH-4. It will be helpful to re-under...

journal_title：BMC biotechnology

authors： Pang J,Wang J,Liu Z,Zhang Q,Qi Q

更新日期：2019-08-27 00:00:00

abstract：BACKGROUND:High recombinant protein productivity in mammalian cell lines is often associated with phenotypic changes in protein content, energy metabolism, and cell growth, but the key determinants that regulate productivity are still not clearly understood. The mammalian target of rapamycin (mTOR) signalling pathway h...

journal_title：BMC biotechnology

authors： Edros R,McDonnell S,Al-Rubeai M

更新日期：2014-02-17 00:00:00

abstract：BACKGROUND:The insect cell line is a critical component in the production of recombinant proteins in the baculovirus expression system and new cell lines hold the promise of increasing both quantity and quality of protein production. RESULTS:Seventy cell lines were established by single-cell cloning from a primary cul...

journal_title：BMC biotechnology

authors： Hashimoto Y,Zhang S,Blissard GW

更新日期：2010-07-05 00:00:00