Abstract:
BACKGROUND:Urease B is an important virulence factor that is required for Helicobacter pylori to colonise the gastric mucosa. Mouse monoclonal antibodies (mAbs) that inhibit urease B enzymatic activity will be useful as vaccines for the prevention and treatment of H. pylori infection. Here, we produced murine mAbs against urease B that neutralize the enzyme's activity. We mapped their epitopes by phage display libraries and investigated the immunogenicity of the selected mimotopes in vivo. RESULTS:The urease B gene was obtained (GenBank accession No. DQ141576) and the recombinant pGEX-4T-1/UreaseB protein was expressed in Escherichia coli as a 92-kDa recombinant fusion protein with glutathione-S-transferase (GST). Five mAbs U001-U005 were produced by a hybridoma-based technique with urease B-GST as an immunogen. Only U001 could inhibit urease B enzymatic activity. Immunoscreening via phage display libraries revealed two different mimotopes of urease B protein; EXXXHDM from ph.D.12-library and EXXXHSM from ph.D.C7C that matched the urease B proteins at 347-353 aa. The antiserum induced by selected phage clones clearly recognised the urease B protein and inhibited its enzymatic activity, which indicated that the phagotope-induced immune responses were antigen specific. CONCLUSIONS:The present work demonstrated that phage-displayed mimotopes were accessible to the mouse immune system and triggered a humoral response. The urease B mimotope could provide a novel and promising approach for the development of a vaccine for the diagnosis and treatment of H. pylori infection.
journal_name
BMC Biotechnoljournal_title
BMC biotechnologyauthors
Li Y,Ning Y,Wang Y,Peng D,Jiang Y,Zhang L,Long M,Luo J,Li Mdoi
10.1186/1472-6750-10-84subject
Has Abstractpub_date
2010-11-30 00:00:00pages
84issn
1472-6750pii
1472-6750-10-84journal_volume
10pub_type
杂志文章abstract:BACKGROUND:Despite positive reports on the efficacy of stem cell therapy for the treatment of cardiovascular disease, the nature of stem cell homing to ischemic tissues remains elusive. RESULTS:We used a mouse model of peripheral tissue ischemia to study the survival and homing capacity of dual reporter gene (eGFP/Luc...
journal_title:BMC biotechnology
pub_type: 杂志文章
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journal_title:BMC biotechnology
pub_type: 杂志文章
doi:10.1186/1472-6750-13-85
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journal_title:BMC biotechnology
pub_type: 杂志文章
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pub_type: 杂志文章
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pub_type: 杂志文章
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journal_title:BMC biotechnology
pub_type: 杂志文章
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更新日期:2018-12-14 00:00:00
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更新日期:2012-01-19 00:00:00
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journal_title:BMC biotechnology
pub_type: 杂志文章
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pub_type: 杂志文章
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journal_title:BMC biotechnology
pub_type: 杂志文章
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journal_title:BMC biotechnology
pub_type: 杂志文章
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journal_title:BMC biotechnology
pub_type: 杂志文章
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更新日期:2016-02-16 00:00:00
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更新日期:2020-11-11 00:00:00
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journal_title:BMC biotechnology
pub_type: 杂志文章
doi:10.1186/1472-6750-12-14
更新日期:2012-04-26 00:00:00
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journal_title:BMC biotechnology
pub_type: 杂志文章
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pub_type: 杂志文章
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更新日期:2015-06-02 00:00:00
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journal_title:BMC biotechnology
pub_type: 杂志文章
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更新日期:2019-01-28 00:00:00
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pub_type: 杂志文章
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更新日期:2016-05-27 00:00:00
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journal_title:BMC biotechnology
pub_type: 杂志文章
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更新日期:2005-02-11 00:00:00
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journal_title:BMC biotechnology
pub_type: 杂志文章
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更新日期:2005-09-01 00:00:00
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更新日期:2016-02-16 00:00:00
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更新日期:2011-06-02 00:00:00
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pub_type: 杂志文章
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更新日期:2012-09-11 00:00:00