A set of dual promoter vectors for high throughput cloning, screening, and protein expression in eukaryotic and prokaryotic systems from a single plasmid.

abstract：BACKGROUND:Circular forms of viral genomic DNA are generated during infection of cells with retroviruses like HIV-1. Such circles are unable to replicate and are eventually lost as a result of cell division, lending support to the prevalent notion that episomal retroviral DNA forms are dead-end products of reverse tran...

journal_title：BMC biotechnology

authors： Moldt B,Staunstrup NH,Jakobsen M,Yáñez-Muñoz RJ,Mikkelsen JG

更新日期：2008-08-09 00:00:00

abstract：BACKGROUND:The antiphospholipid syndrome (APS), characterised by recurrent miscarriage and thrombosis, is a significant cause of morbidity and mortality. Domain I (DI) of human beta 2 glycoprotein I (beta2GPI) is thought to contain crucial antibody binding epitopes for antiphospholipid antibodies (aPL), which are criti...

journal_title：BMC biotechnology

authors： Ioannou Y,Giles I,Lambrianides A,Richardson C,Pearl LH,Latchman DS,Isenberg DA,Rahman A

更新日期：2006-02-10 00:00:00

abstract：BACKGROUND:The identification of known mutations in a cell population is important for clinical applications and basic cancer research. In this work an immobilized form of the polymerase chain reaction, referred to as polony technology, was used to detect mutations as well as gene deletions, resulting in loss of hetero...

journal_title：BMC biotechnology

authors： Butz J,Wickstrom E,Edwards J

更新日期：2003-07-23 00:00:00

abstract：BACKGROUND:Insect resistance in crops represents a main challenge for agriculture. Transgenic approaches based on proteins displaying insect resistance properties are widely used as efficient breeding strategies. To extend the spectrum of targeted pathogens and overtake the development of resistance, molecular evolutio...

journal_title：BMC biotechnology

authors： Prado GS,Bamogo PKA,de Abreu JAC,Gillet FX,Dos Santos VO,Silva MCM,Brizard JP,Bemquerer MP,Bangratz M,Brugidou C,Sérémé D,Grossi-de-Sa MF,Lacombe S

更新日期：2019-03-08 00:00:00

abstract：BACKGROUND:The use of small interfering RNA (siRNA) molecules in animals to achieve double-stranded RNA-mediated interference (RNAi) has recently emerged as a powerful method of sequence-specific gene knockdown. As DNA-based expression of short hairpin RNA (shRNA) for RNAi may offer some advantages over chemical and in...

journal_title：BMC biotechnology

authors： Lambeth LS,Moore RJ,Muralitharan M,Dalrymple BP,McWilliam S,Doran TJ

更新日期：2005-05-11 00:00:00

abstract：BACKGROUND:Hydrophobic polystyrene is the most common material for solid phase immunoassay. Proteins are immobilized on polystyrene by passive adsorption, which often causes considerable denaturation. Biological macromolecules were found to better retain their functional activity when immobilized on hydrophilic materia...

journal_title：BMC biotechnology

authors： Shmanai VV,Nikolayeva TA,Vinokurova LG,Litoshka AA

更新日期：2001-01-01 00:00:00

abstract：BACKGROUND:Heat-stable antifungal factor (HSAF) is a newly identified broad-spectrum antifungal antibiotic from the biocontrol agent Lysobacter enzymogenes and is regarded as a potential biological pesticide, due to its novel mode of action. However, the production level of HSAF is quite low, and little research has re...

journal_title：BMC biotechnology

authors： Tang B,Sun C,Zhao Y,Xu H,Xu G,Liu F

更新日期：2018-10-24 00:00:00

abstract：BACKGROUND:Microarray technology has been used extensively over the past 10 years for assessing gene expression, and has facilitated precise genetic profiling of everything from tumors to small molecule drugs. By contrast, arraying cell membranes in a manner which preserves their ability to mediate biochemical processe...

journal_title：BMC biotechnology

authors： Yamazaki V,Sirenko O,Schafer RJ,Nguyen L,Gutsmann T,Brade L,Groves JT

更新日期：2005-06-16 00:00:00

abstract：BACKGROUND:Porcine reproductive and respiratory syndrome virus (PRRSV) causes major economic losses in the pig industry worldwide. In vivo, the virus infects a subpopulation of tissue macrophages. In vitro, PRRSV only replicates in primary pig macrophages and African green monkey kidney derived cells, such as Marc-145....

journal_title：BMC biotechnology

authors： Delrue I,Van Gorp H,Van Doorsselaere J,Delputte PL,Nauwynck HJ

更新日期：2010-06-29 00:00:00

abstract：BACKGROUND:Dengue is a public health problem of global significance for which there is neither an effective antiviral therapy nor a preventive vaccine. It is a mosquito-borne viral disease, caused by dengue (DEN) viruses, which are members of the Flaviviridae family. There are four closely related serotypes, DEN-1, DEN...

journal_title：BMC biotechnology

authors： Khanam S,Rajendra P,Khanna N,Swaminathan S

更新日期：2007-02-15 00:00:00

abstract：BACKGROUND:The CRISPR/Cas9 system is increasingly used for gene inactivation in mouse zygotes, but homology-directed mutagenesis and use of inbred embryos are less established. In particular, Rosa26 knock-in alleles for the insertion of transgenes in a genomic 'safe harbor' site, have not been produced. Here we applied...

journal_title：BMC biotechnology

authors： Chu VT,Weber T,Graf R,Sommermann T,Petsch K,Sack U,Volchkov P,Rajewsky K,Kühn R

更新日期：2016-01-16 00:00:00

abstract：BACKGROUND:Social amoeba, Dictyostelium discoideum, is a well-established model organism for studying cellular physiology and developmental pattern formation. Its haploid genome facilitates functional analysis of genes by a single round of mutagenesis including targeted disruption. Although the efficient generation of ...

journal_title：BMC biotechnology

authors： Mukai A,Ichiraku A,Horikawa K

更新日期：2016-04-14 00:00:00

abstract：BACKGROUND:The ketoisovalerate reductase (EC 1.2.7.7 ) is required for the formation of beauvericin via the nonribosomal peptide synthetase biosynthetic pathway. It catalyzes the NADPH-specific reduction of ketoisovaleric acid to hydroxyisovalerate. However, little is known about the bioinformatics' data about the 2-Ki...

journal_title：BMC biotechnology

authors： Zhang T,Jia X,Zhuo Y,Liu M,Gao H,Liu J,Zhang L

更新日期：2012-08-23 00:00:00

abstract：BACKGROUND:More than a dozen of fungal immunomodulatory proteins (FIPs) have been identified to date, most of which are from Ganoderma species. However, little is known about the similarities and differences between different Ganoderma FIPs' bioactivities. In the current study, two FIP genes termed FIP-gap1 and FIP-gap...

journal_title：BMC biotechnology

authors： Qu ZW,Zhou SY,Guan SX,Gao R,Duan ZW,Zhang X,Sun WY,Fan WL,Chen SS,Chen LJ,Lin JW,Ruan YY

更新日期：2018-12-14 00:00:00

abstract：BACKGROUND:Mesenchymal stem cells (MSCs) are of great interest in bone regenerative medicine due to their osteogenic potential and trophic effects. However, challenges to large-scale production of MSCs can hinder the translation of MSC therapies. 3D Microcarrier (MC)-based MSC culture presents a scalable and cost-effec...

journal_title：BMC biotechnology

authors： Shekaran A,Sim E,Tan KY,Chan JK,Choolani M,Reuveny S,Oh S

更新日期：2015-10-31 00:00:00

abstract：BACKGROUND:The photorespiratory nitrogen cycle in C₃ plants involves an extensive diversion of carbon and nitrogen away from the direct pathways of assimilation. The liberated ammonia is re-assimilated, but up to 25% of the carbon may be released into the atmosphere as CO₂. Because of the loss of CO₂ and high energy co...

journal_title：BMC biotechnology

authors： Carvalho Jde F,Madgwick PJ,Powers SJ,Keys AJ,Lea PJ,Parry MA

更新日期：2011-11-21 00:00:00

abstract：BACKGROUND:Multi-copy gene integration into microbial genomes is a conventional tool for obtaining improved gene expression. For Penicillium chrysogenum, the fungal producer of the beta-lactam antibiotic penicillin, many production strains carry multiple copies of the penicillin biosynthesis gene cluster. This discover...

journal_title：BMC biotechnology

authors： Ziemons S,Koutsantas K,Becker K,Dahlmann T,Kück U

更新日期：2017-02-16 00:00:00

abstract：BACKGROUND:Increasing cell membrane permeability to water and cryoprotectants is critical for the successful cryopreservation of cells with large volumes. Artificial expression of water-selective aquaporins or aquaglyceroporins (GLPs), such as mammalian aquaporin-3 (AQP3), enhances cell permeability to water and cryopr...

journal_title：BMC biotechnology

authors： Chauvigné F,Lubzens E,Cerdà J

更新日期：2011-04-08 00:00:00

abstract：BACKGROUND:Expressed Sequence Tag (EST) sequences are widely used in applications such as genome annotation, gene discovery and gene expression studies. However, some of GenBank dbEST sequences have proven to be "unclean". Identification of cDNA termini/ends and their structures in raw ESTs not only facilitates data qu...

journal_title：BMC biotechnology

authors： Zhou S,Ji G,Liu X,Li P,Moler J,Karro JE,Liang C

更新日期：2012-05-03 00:00:00

abstract：BACKGROUND:Genetically encoded sensors developed on the basis of green fluorescent protein (GFP)-like proteins are becoming more and more popular instruments for monitoring cellular analytes and enzyme activities in living cells and transgenic organisms. In particular, a number of Ca2+ sensors have been developed, eith...

journal_title：BMC biotechnology

authors： Souslova EA,Belousov VV,Lock JG,Strömblad S,Kasparov S,Bolshakov AP,Pinelis VG,Labas YA,Lukyanov S,Mayr LM,Chudakov DM

更新日期：2007-06-29 00:00:00

abstract：BACKGROUND:Baculovirus, which has a width of 40 nm and a length of 250-300 nm, can display functional peptides, receptors and antigens on its surface by their fusion with a baculovirus envelop protein, GP64. In addition, some transmembrane proteins can be displayed without GP64 fusion, using the native transmembrane do...

journal_title：BMC biotechnology

authors： Kato T,Suzuki F,Park EY

更新日期：2011-06-02 00:00:00

abstract：BACKGROUND:Clostridial co-culture containing cellulolytic and solventogenic species is a potential consolidated bioprocessing (CBP) approach for producing biochemicals and biofuels from cellulosic biomass. It has been demonstrated that the rate of cellulose utilization in the co-culture of Clostridium acetobutylicum an...

journal_title：BMC biotechnology

authors： Salimi F,Mahadevan R

更新日期：2013-11-04 00:00:00

abstract：BACKGROUND:Construction of high quality cDNA libraries from the usually low amounts of eukaryotic mRNA extracted from environmental samples is essential in functional metatranscriptomics for the selection of functional, full-length genes encoding proteins of interest. Many of the inserts in libraries constructed by sta...

journal_title：BMC biotechnology

authors： Yadav RK,Barbi F,Ziller A,Luis P,Marmeisse R,Reddy MS,Fraissinet-Tachet L

更新日期：2014-09-03 00:00:00

abstract：BACKGROUND:Clustered Regularly Interspaced Short Palindromic Repeats/CRISPR associated protein 9 (CRISPR/Cas9) has been wildly used to generate gene knockout models through inducing indels causing frame-shift. However, there are few studies concerning the post-transcript effects caused by CRISPR-mediated genome editing...

journal_title：BMC biotechnology

authors： Tang JX,Chen D,Deng SL,Li J,Li Y,Fu Z,Wang XX,Zhang Y,Chen SR,Liu YX

更新日期：2018-10-03 00:00:00

abstract：BACKGROUND:β-Fructofuranosidases (or invertases) catalyse the commercially-important biotransformation of sucrose into short-chain fructooligosaccharides with wide-scale application as a prebiotic in the functional foods and pharmaceutical industries. RESULTS:We identified a β-fructofuranosidase gene (CmINV) from a Ce...

journal_title：BMC biotechnology

authors： van Wyk N,Trollope KM,Steenkamp ET,Wingfield BD,Volschenk H

更新日期：2013-11-14 00:00:00

abstract：BACKGROUND:Serine hydroxymethyltransferase (SHMT) is the key enzyme in L-serine enzymatic production, suggesting the importance of obtaining a SHMT with high activity. RESULTS:Here, a novel SHMT gene, glyA, was obtained through degenerate oligonucleotide-primed PCR and encoded a novel SHMT with 54.3% similarity to the...

journal_title：BMC biotechnology

authors： Jiang W,Chen L,Hu N,Yuan S,Li B,Liu Z

更新日期：2014-11-14 00:00:00

abstract：BACKGROUND:Whole genome amplification (WGA) promises to eliminate practical molecular genetic analysis limitations associated with genomic DNA (gDNA) quantity. We evaluated the performance of multiple displacement amplification (MDA) WGA using gDNA extracted from lymphoblastoid cell lines (N = 27) with a range of start...

journal_title：BMC biotechnology

authors： Bergen AW,Qi Y,Haque KA,Welch RA,Chanock SJ

更新日期：2005-09-16 00:00:00

abstract：BACKGROUND:New high-throughput sequencing technologies promise a very sensitive and high-resolution analysis of DNA methylation patterns in quantitative terms. However, a detailed and comprehensive comparison with existing validated DNA methylation analysis methods is not yet available. Therefore, a systematic cross-va...

journal_title：BMC biotechnology

authors： Potapova A,Albat C,Hasemeier B,Haeussler K,Lamprecht S,Suerbaum S,Kreipe H,Lehmann U

更新日期：2011-01-14 00:00:00

abstract：BACKGROUND:The cattle ticks, Boophilus spp., affect cattle production in tropical and subtropical regions of the world. Tick vaccines constitute a cost-effective and environmentally friendly alternative to tick control. The recombinant B. microplus Bm86 protective antigen has been shown to protect cattle against tick i...

journal_title：BMC biotechnology

authors： Canales M,Almazán C,Naranjo V,Jongejan F,de la Fuente J

更新日期：2009-03-31 00:00:00

abstract：BACKGROUND:The ubiquitin protein is present in all eukaryotic cells and promoters from ubiquitin genes are good candidates to regulate the constitutive expression of transgenes in plants. Therefore, two switchgrass (Panicum virgatum L.) ubiquitin genes (PvUbi1 and PvUbi2) were cloned and characterized. Reporter constru...

journal_title：BMC biotechnology

authors： Mann DG,King ZR,Liu W,Joyce BL,Percifield RJ,Hawkins JS,LaFayette PR,Artelt BJ,Burris JN,Mazarei M,Bennetzen JL,Parrott WA,Stewart CN Jr

更新日期：2011-07-11 00:00:00