Efficient production of heat-stable antifungal factor through integrating statistical optimization with a two-stage temperature control strategy in Lysobacter enzymogenes OH11.

abstract：BACKGROUND:Rapid, robust and reversible induction of transgene expression would significantly facilitate cancer gene therapy as well as allow the in vivo functional study of newly discovered genes in tumor formation and progression. The popularity of the ecdysone inducible gene switch system has led us to investigate w...

journal_title：BMC biotechnology

authors： Karns LR,Kisielewski A,Gulding KM,Seraj JM,Theodorescu D

更新日期：2001-01-01 00:00:00

abstract：BACKGROUND:Analysis of protein-protein interactions (PPIs) is a valuable approach for the characterization of huge networks of protein complexes or proteins of unknown function. Co-immunoprecipitation (coIP) using affinity resins coupled to protein A/G is the most widely used method for PPI detection. However, this tra...

journal_title：BMC biotechnology

authors： Liu Q,Chen Q,Wang J,Zhang Y,Zhou Y,Lin C,He W,He F,Xu D

更新日期：2010-10-28 00:00:00

abstract：BACKGROUND:Copper is an essential chemical element for life as it is a part of prosthetic groups of enzymes including super oxide dismutase and cytochrome c oxidase; however, it is also toxic at high concentrations. Here, we present the trade-off of copper availability and growth inhibition of a common host used for co...

journal_title：BMC biotechnology

authors： Balakumaran PA,Förster J,Zimmermann M,Charumathi J,Schmitz A,Czarnotta E,Lehnen M,Sudarsan S,Ebert BE,Blank LM,Meenakshisundaram S

更新日期：2016-02-20 00:00:00

abstract：BACKGROUND:There are significant differences in the culture conditions between small-scale screenings and large-scale fermentation processes. Production processes are usually conducted in fed-batch cultivation mode with active pH-monitoring and control. In contrast, screening experiments in shake flasks are usually con...

journal_title：BMC biotechnology

authors： Scheidle M,Dittrich B,Klinger J,Ikeda H,Klee D,Büchs J

更新日期：2011-03-23 00:00:00

abstract：BACKGROUND:Methylated CpG dinucleotides in promoters are associated with the loss of gene expression in recombinant Chinese hamster ovary (CHO) cells during large-scale commercial manufacturing. We evaluated a promoter devoid of CpG dinucleotides, CpGfree, in parallel with a similar CpG containing promoter, CpGrich, fo...

journal_title：BMC biotechnology

authors： Ho SC,Koh EY,Soo BP,Mariati,Chao SH,Yang Y

更新日期：2016-10-18 00:00:00

abstract：BACKGROUND:Mesenchymal stem cells (MSCs) are an attractive source of adult stem cells for therapeutic application in clinical study. Genetic modification of MSCs with beneficial genes makes them more effective for therapeutic use. However, it is difficult to transduce genes into MSCs by common transfection methods, esp...

journal_title：BMC biotechnology

authors： Lim JY,Park SH,Jeong CH,Oh JH,Kim SM,Ryu CH,Park SA,Ahn JG,Oh W,Jeun SS,Chang JW

更新日期：2010-05-13 00:00:00

abstract：: ...

journal_title：BMC biotechnology

authors： Devilder MC,Moyon M,Gautreau-Rolland L,Navet B,Perroteau J,Delbos F,Gesnel MC,Breathnach R,Saulquin X

更新日期：2019-02-18 00:00:00

abstract：BACKGROUND:Hydrophobic polystyrene is the most common material for solid phase immunoassay. Proteins are immobilized on polystyrene by passive adsorption, which often causes considerable denaturation. Biological macromolecules were found to better retain their functional activity when immobilized on hydrophilic materia...

journal_title：BMC biotechnology

authors： Shmanai VV,Nikolayeva TA,Vinokurova LG,Litoshka AA

更新日期：2001-01-01 00:00:00

abstract：BACKGROUND:Polysialic acid (polySia) is a carbohydrate modification of the neural cell adhesion molecule (NCAM), which is implicated in neural differentiation and plays an important role in tumor development and metastasis. Polysialylation of NCAM is mediated by two Golgi-resident polysialyltransferases (polyST) ST8Sia...

journal_title：BMC biotechnology

authors： Somplatzki S,Mühlenhoff M,Kröger A,Gerardy-Schahn R,Böldicke T

更新日期：2017-05-12 00:00:00

abstract：BACKGROUND:Expression vector engineering technology is one of the most convenient and timely method for cell line development to meet the rising demand of novel production cell line with high productivity. Destabilization of dihydrofolate reductase (dhfr) selection marker by addition of AU-rich elements and murine orni...

journal_title：BMC biotechnology

authors： Chin CL,Chin HK,Chin CS,Lai ET,Ng SK

更新日期：2015-06-02 00:00:00

abstract：BACKGROUND:DsRed the red fluorescent protein (RFP) isolated from Discosoma sp. coral holds much promise as a genetically and spectrally distinct alternative to green fluorescent protein (GFP) for application in mice. Widespread use of DsRed has been hampered by several issues resulting in the inability to establish and...

journal_title：BMC biotechnology

authors： Long JZ,Lackan CS,Hadjantonakis AK

更新日期：2005-07-04 00:00:00

abstract：BACKGROUND:In a previous study, anthocyanin levels in potato plants were increased by manipulating genes connected with the flavonoid biosynthesis pathway. However, starch content and tuber yield were dramatically reduced in the transgenic plants, which over-expressed dihydroflavonol reductase (DFR). RESULTS:Transgeni...

journal_title：BMC biotechnology

authors： Aksamit-Stachurska A,Korobczak-Sosna A,Kulma A,Szopa J

更新日期：2008-03-05 00:00:00

abstract：BACKGROUND:Recombinant factor VIII (FVIII), used for haemophilia A therapy, is one of the most challenging among the therapeutic proteins produced in heterologous expression systems. Deletion variant of FVIII, in which the entire domain B is replaced by a short linker peptide, was approved for medical use. Efficacy and...

journal_title：BMC biotechnology

authors： Orlova NA,Kovnir SV,Gabibov AG,Vorobiev II

更新日期：2017-03-24 00:00:00

abstract：BACKGROUND:Iron is an essential element in several pathways of microbial metabolism, and therefore low iron toxicity is expected on the usage of Fe nanoparticles (NPs). This study aims to determine the effect of Fe NPs on biosurfactant production by marine actinobacterium Nocardiopsis sp. MSA13A under solid state cultu...

journal_title：BMC biotechnology

authors： Kiran GS,Nishanth LA,Priyadharshini S,Anitha K,Selvin J

更新日期：2014-05-21 00:00:00

abstract：BACKGROUND:Gene therapy continues to hold great potential for treating many different types of disease and dysfunction. Safe and efficient techniques for gene transfer and expression in vivo are needed to enable gene therapeutic strategies to be effective in patients. Currently, the most commonly used methods employ re...

journal_title：BMC biotechnology

authors： Xia J,Martinez A,Daniell H,Ebert SN

更新日期：2011-06-02 00:00:00

abstract：BACKGROUND:Natural rubber produced by plants, known as polyisoprene, is the most widely used isoprenoid polymer. Plant polyisoprenes can be classified into two types; cis-polyisoprene and trans-polyisoprene, depending on the type of polymerization of the isoprene unit. More than 2000 species of higher plants produce la...

journal_title：BMC biotechnology

authors： Chen R,Harada Y,Bamba T,Nakazawa Y,Gyokusen K

更新日期：2012-10-30 00:00:00

abstract：BACKGROUND:Serine hydroxymethyltransferase (SHMT) is the key enzyme in L-serine enzymatic production, suggesting the importance of obtaining a SHMT with high activity. RESULTS:Here, a novel SHMT gene, glyA, was obtained through degenerate oligonucleotide-primed PCR and encoded a novel SHMT with 54.3% similarity to the...

journal_title：BMC biotechnology

authors： Jiang W,Chen L,Hu N,Yuan S,Li B,Liu Z

更新日期：2014-11-14 00:00:00

abstract：BACKGROUND:Protein cross-coupling reactions demand high yields, especially if the products are intended for bioanalytics, like enzyme-linked immunosorbent assays. Amongst other factors, the coupling yield depends on the concentration of the proteins being used for coupling. Protein supercharging of enzymes can increase...

journal_title：BMC biotechnology

authors： Prasse AA,Zauner T,Büttner K,Hoffmann R,Zuchner T

更新日期：2014-10-18 00:00:00

abstract：BACKGROUND:Dissolved oxygen tension (DOT) is hardly constant and homogenously distributed in a bioreactor, which can have a negative impact in the metabolism and product synthesis. However, the effects of DOT on plasmid DNA (pDNA) production and quality have not been thoroughly investigated. In the present study, the e...

journal_title：BMC biotechnology

authors： Jaén KE,Sigala JC,Olivares-Hernández R,Niehaus K,Lara AR

更新日期：2017-07-04 00:00:00

abstract：BACKGROUND:Engineered proteins, with non-immunoglobulin scaffolds, have become an important alternative to antibodies in many biotechnical and therapeutic applications. When compared to antibodies, tailored proteins may provide advantageous properties such as a smaller size or a more stable structure. RESULTS:Avidin i...

journal_title：BMC biotechnology

authors： Riihimäki TA,Hiltunen S,Rangl M,Nordlund HR,Määttä JA,Ebner A,Hinterdorfer P,Kulomaa MS,Takkinen K,Hytönen VP

更新日期：2011-06-09 00:00:00

abstract：BACKGROUND:Splicing of DNA molecules is an important task in molecular biology that facilitates cloning, mutagenesis and creation of chimeric genes. Mutagenesis and DNA splicing techniques exist, some requiring restriction enzymes, and others utilize staggered reannealing approaches. RESULTS:A method for DNA splicing ...

journal_title：BMC biotechnology

authors： Ailenberg M,Goldenberg NM,Silverman M

更新日期：2005-09-01 00:00:00

abstract：BACKGROUND:Chinese hamster ovary (CHO) cells have become the host of choice for the production of recombinant proteins, due to their capacity for correct protein folding, assembly, and posttranslational modifications. The most widely used system for recombinant proteins is the gene amplification procedure that uses the...

journal_title：BMC biotechnology

authors： Jamnikar U,Nikolic P,Belic A,Blas M,Gaser D,Francky A,Laux H,Blejec A,Baebler S,Gruden K

更新日期：2015-10-23 00:00:00

abstract：BACKGROUND:Xenoantigens are a major source of concern with regard to the success of interspecific xenografts. GGTA1 encodes α1,3-galactosyltransferase, which is essential for the biosynthesis of galactosyl-alpha 1,3-galactose, the major xenoantigen causing hyperacute rejection. GGTA1-modified pigs, therefore, are promi...

journal_title：BMC biotechnology

authors： Tanihara F,Hirata M,Nguyen NT,Sawamoto O,Kikuchi T,Doi M,Otoi T

更新日期：2020-08-18 00:00:00

abstract：BACKGROUND:Prostate cancer is the most-diagnosed non-skin cancer among males in the US, and the second leading cause of cancer-related death. Current methods of treatment and diagnosis are not specific for the disease. This work identified an antibody fragment that binds selectively to a molecule on the surface of andr...

journal_title：BMC biotechnology

authors： Williams RM,Hajiran CJ,Nayeem S,Sooter LJ

更新日期：2014-09-03 00:00:00

abstract：BACKGROUND:In spite of a great number of monomeric fluorescent proteins developed in the recent years, the reported fluorescent protein-based FRET pairs are still characterized by a number of disadvantageous features, complicating their use as reporters in cell biology and for high-throughput cell-based screenings. RE...

journal_title：BMC biotechnology

authors： Shcherbo D,Souslova EA,Goedhart J,Chepurnykh TV,Gaintzeva A,Shemiakina II,Gadella TW,Lukyanov S,Chudakov DM

更新日期：2009-03-25 00:00:00

abstract：BACKGROUND:A modification of a standard method of fluorescence in situ hybridisation (FISH) is described, by which a combination of several substrates and probes on single microscope slides enables more accurate comparisons of the distribution and abundance of chromosomal sequences and improves the relatively low throu...

journal_title：BMC biotechnology

authors： Hasterok R,Dulawa J,Jenkins G,Leggett M,Langdon T

更新日期：2006-03-20 00:00:00

abstract：BACKGROUND:Dengue is a public health problem of global significance for which there is neither an effective antiviral therapy nor a preventive vaccine. It is a mosquito-borne viral disease, caused by dengue (DEN) viruses, which are members of the Flaviviridae family. There are four closely related serotypes, DEN-1, DEN...

journal_title：BMC biotechnology

authors： Khanam S,Rajendra P,Khanna N,Swaminathan S

更新日期：2007-02-15 00:00:00

abstract：BACKGROUND:Asian rust (Phakopsora pachyrhizi) is a common disease in Brazilian soybean fields and it is difficult to control. To identify a biochemical candidate with potential to combat this disease, a new chitinase-like xylanase inhibitor protein (XIP) from coffee (Coffea arabica) (CaclXIP) leaves was cloned into the...

journal_title：BMC biotechnology

authors： Vasconcelos EA,Santana CG,Godoy CV,Seixas CD,Silva MS,Moreira LR,Oliveira-Neto OB,Price D,Fitches E,Filho EX,Mehta A,Gatehouse JA,Grossi-De-Sa MF

更新日期：2011-02-07 00:00:00

abstract：BACKGROUND:Protein glycosylation is of fundamental importance in many biological systems. The discovery of N-glycosylation in bacteria and the functional expression of the N-oligosaccharyltransferase PglB of Campylobacter jejuni in Escherichia coli enabled the production of engineered glycoproteins and the study of the...

journal_title：BMC biotechnology

authors： Ihssen J,Kowarik M,Wiesli L,Reiss R,Wacker M,Thöny-Meyer L

更新日期：2012-09-24 00:00:00

abstract：BACKGROUND:To deal with the increasingly severe energy crisis and environmental consequences, biofuels and biochemicals generated from renewable resources could serve as a promising alternative for replacing petroleum as a source of fuel and chemicals, among which isoprene (2-methyl-1,3-butadiene) in particular is of g...

journal_title：BMC biotechnology

authors： Yang J,Nie Q,Liu H,Xian M,Liu H

更新日期：2016-01-20 00:00:00