Intracellular delivery of messenger RNA by recombinant PP7 virus-like particles carrying low molecular weight protamine.

Abstract:

BACKGROUND:Cell-penetrating peptides (CPPs) have been widely used as carriers to transport different molecules into living cells, whereas messenger RNAs (mRNAs) have been utilized as target molecules for the prevention and treatment of various diseases. However, the instability of CPPs and mRNAs has limited their application. Bacteriophage PP7 virus-like particles (VLPs) may protect peptides and RNAs from degradation through displaying foreign peptides on their surface and encapsidating RNA linked with the pac site. RESULTS:In this study, the cDNA of the PP7 coat protein single-chain dimer carrying low molecular weight protamine (LMWP) and the cDNA of green fluorescent protein (GFP) were inserted into two multiple cloning sites of pETDuet-1, respectively. PP7 VLPs carrying the LMWP peptide and GFP mRNA were subsequently expressed in Escherichia coli BL21 (DE3) with high yield and thermal stability, and were easily purified. The VLPs were also non-replicative, non-infectious, and non-toxic. Moreover, they penetrated the mouse prostate cancer cells RM-1 after 24 h incubation. Last, PP7 VLPs carrying the LMWP could encapsidate the GFP mRNA, which was translated into mature protein in mammalian cells. CONCLUSIONS:Recombinant PP7 VLPs can be used simultaneously as a targeted delivery vector for both peptides and mRNA due to their abilities to package RNA and display peptides.

journal_name

BMC Biotechnol

journal_title

BMC biotechnology

authors

Sun Y,Sun Y,Zhao R,Gao K

doi

10.1186/s12896-016-0274-9

subject

Has Abstract

pub_date

2016-05-28 00:00:00

pages

46

issue

1

issn

1472-6750

pii

10.1186/s12896-016-0274-9

journal_volume

16

pub_type

杂志文章
  • Description of a PCR-based technique for DNA splicing and mutagenesis by producing 5' overhangs with run through stop DNA synthesis utilizing Ara-C.

    abstract:BACKGROUND:Splicing of DNA molecules is an important task in molecular biology that facilitates cloning, mutagenesis and creation of chimeric genes. Mutagenesis and DNA splicing techniques exist, some requiring restriction enzymes, and others utilize staggered reannealing approaches. RESULTS:A method for DNA splicing ...

    journal_title:BMC biotechnology

    pub_type: 杂志文章

    doi:10.1186/1472-6750-5-23

    authors: Ailenberg M,Goldenberg NM,Silverman M

    更新日期:2005-09-01 00:00:00

  • Degradation or excretion of quantum dots in mouse embryonic stem cells.

    abstract:BACKGROUND:Quantum dots (QDs) have been considered as a new and efficient probe for labeling cells non-invasively in vitro and in vivo, but fairly little is known about how QDs are eliminated from cells after labeling. The purpose of this study is to investigate the metabolism of QDs in different type of cells. RESULT...

    journal_title:BMC biotechnology

    pub_type: 杂志文章

    doi:10.1186/1472-6750-10-36

    authors: Pi QM,Zhang WJ,Zhou GD,Liu W,Cao Y

    更新日期:2010-05-06 00:00:00

  • Rapid high-yield expression of a candidate influenza vaccine based on the ectodomain of M2 protein linked to flagellin in plants using viral vectors.

    abstract:BACKGROUND:The extracellular domain of matrix protein 2 (M2e) of influenza A virus is a promising target for the development of a universal vaccine against influenza because M2e sequences are highly conserved among human influenza A strains. However, native M2e is poorly immunogenic, but its immunogenicity can be incre...

    journal_title:BMC biotechnology

    pub_type: 杂志文章

    doi:10.1186/s12896-015-0164-6

    authors: Mardanova ES,Kotlyarov RY,Kuprianov VV,Stepanova LA,Tsybalova LM,Lomonosoff GP,Ravin NV

    更新日期:2015-05-29 00:00:00

  • A novel adenoviral vector carrying an all-in-one Tet-On system with an autoregulatory loop for tight, inducible transgene expression.

    abstract:BACKGROUND:One of the most commonly used vectors for gene therapy is the adenoviral vector; its ability to tightly regulate transgene expression is critical for optimizing therapeutic outcomes. The tetracycline-regulated system (especially the Tet-On system) for gene expression is one of the most valuable tools for con...

    journal_title:BMC biotechnology

    pub_type: 杂志文章

    doi:10.1186/s12896-015-0121-4

    authors: Chen H,Wang D,Xia R,Mao Q,Xia H

    更新日期:2015-02-13 00:00:00

  • Improved detection of Escherichia coli and coliform bacteria by multiplex PCR.

    abstract:BACKGROUND:The presence of coliform bacteria is routinely assessed to establish the microbiological safety of water supplies and raw or processed foods. Coliforms are a group of lactose-fermenting Enterobacteriaceae, which most likely acquired the lacZ gene by horizontal transfer and therefore constitute a polyphyletic...

    journal_title:BMC biotechnology

    pub_type: 杂志文章

    doi:10.1186/s12896-015-0168-2

    authors: Molina F,López-Acedo E,Tabla R,Roa I,Gómez A,Rebollo JE

    更新日期:2015-06-04 00:00:00

  • Heterologous expression, purification and characterization of nitrilase from Aspergillus niger K10.

    abstract:BACKGROUND:Nitrilases attract increasing attention due to their utility in the mild hydrolysis of nitriles. According to activity and gene screening, filamentous fungi are a rich source of nitrilases distinct in evolution from their widely examined bacterial counterparts. However, fungal nitrilases have been less explo...

    journal_title:BMC biotechnology

    pub_type: 杂志文章,收录出版

    doi:10.1186/1472-6750-11-2

    authors: Kaplan O,Bezouška K,Plíhal O,Ettrich R,Kulik N,Vaněk O,Kavan D,Benada O,Malandra A,Sveda O,Veselá AB,Rinágelová A,Slámová K,Cantarella M,Felsberg J,Dušková J,Dohnálek J,Kotik M,Křen V,Martínková L

    更新日期:2011-01-06 00:00:00

  • Setting up a platform for plant-based influenza virus vaccine production in South Africa.

    abstract:BACKGROUND:During a global influenza pandemic, the vaccine requirements of developing countries can surpass their supply capabilities, if these exist at all, compelling them to rely on developed countries for stocks that may not be available in time. There is thus a need for developing countries in general to produce t...

    journal_title:BMC biotechnology

    pub_type: 杂志文章

    doi:10.1186/1472-6750-12-14

    authors: Mortimer E,Maclean JM,Mbewana S,Buys A,Williamson AL,Hitzeroth II,Rybicki EP

    更新日期:2012-04-26 00:00:00

  • Characterization of a cold-active esterase from Serratia sp. and improvement of thermostability by directed evolution.

    abstract:BACKGROUND:In recent years, cold-active esterases have received increased attention due to their attractive properties for some industrial applications such as high catalytic activity at low temperatures. RESULTS:An esterase-encoding gene (estS, 909 bp) from Serratia sp. was identified, cloned and expressed in Escheri...

    journal_title:BMC biotechnology

    pub_type: 杂志文章

    doi:10.1186/s12896-016-0235-3

    authors: Jiang H,Zhang S,Gao H,Hu N

    更新日期:2016-01-22 00:00:00

  • Similarity of recombinant human perlecan domain 1 by alternative expression systems bioactive heterogenous recombinant human perlecan D1.

    abstract:BACKGROUND:Heparan sulfate glycosaminoglycans are diverse components of certain proteoglycans and are known to interact with growth factors as a co-receptor necessary to induce signalling and growth factor activity. In this report we characterize heterogeneously glycosylated recombinant human perlecan domain 1 (HSPG2 a...

    journal_title:BMC biotechnology

    pub_type: 杂志文章

    doi:10.1186/1472-6750-10-66

    authors: Ellis AL,Pan W,Yang G,Jones K,Chuang C,Whitelock JM,DeCarlo AA

    更新日期:2010-09-09 00:00:00

  • In vitro cell cultures obtained from different explants of Corylus avellana produce Taxol and taxanes.

    abstract:BACKGROUND:Taxol is an effective antineoplastic agent, originally extracted from the bark of Taxus brevifolia with a low yield. Many attempts have been made to produce Taxol by chemical synthesis, semi-synthesis and plant tissue cultures. However, to date, the availability of this compound is not sufficient to satisfy ...

    journal_title:BMC biotechnology

    pub_type: 杂志文章

    doi:10.1186/1472-6750-6-45

    authors: Bestoso F,Ottaggio L,Armirotti A,Balbi A,Damonte G,Degan P,Mazzei M,Cavalli F,Ledda B,Miele M

    更新日期:2006-12-06 00:00:00

  • A Sleeping Beauty DNA transposon-based genetic sensor for functional screening of vitamin D3 analogues.

    abstract:BACKGROUND:Analogues of vitamin D3 are extensively used in the treatment of various illnesses, such as osteoporosis, inflammatory skin diseases, and cancer. Functional testing of new vitamin D3 analogues and formulations for improved systemic and topical administration is supported by sensitive screening methods that a...

    journal_title:BMC biotechnology

    pub_type: 杂志文章

    doi:10.1186/1472-6750-11-33

    authors: Staunstrup NH,Sharma N,Bak RO,Svensson L,Petersen TK,Aarenstrup L,Kristiansen K,Bolund L,Mikkelsen JG

    更新日期:2011-04-07 00:00:00

  • Synergistic interaction of hTGF-β3 with hBMP-6 promotes articular cartilage formation in chitosan scaffolds with hADSCs: implications for regenerative medicine.

    abstract:BACKGROUND:Human TGF-β3 has been used in many studies to induce genes coding for typical cartilage matrix components and accelerate chondrogenic differentiation, making it the standard constituent in most cultivation media used for the assessment of chondrogenesis associated with various stem cell types on carrier matr...

    journal_title:BMC biotechnology

    pub_type: 杂志文章

    doi:10.1186/s12896-020-00641-y

    authors: Huang Y,Seitz D,Chevalier Y,Müller PE,Jansson V,Klar RM

    更新日期:2020-08-27 00:00:00

  • Design and characterization of genetically engineered zebrafish aquaporin-3 mutants highly permeable to the cryoprotectant ethylene glycol.

    abstract:BACKGROUND:Increasing cell membrane permeability to water and cryoprotectants is critical for the successful cryopreservation of cells with large volumes. Artificial expression of water-selective aquaporins or aquaglyceroporins (GLPs), such as mammalian aquaporin-3 (AQP3), enhances cell permeability to water and cryopr...

    journal_title:BMC biotechnology

    pub_type: 杂志文章

    doi:10.1186/1472-6750-11-34

    authors: Chauvigné F,Lubzens E,Cerdà J

    更新日期:2011-04-08 00:00:00

  • Improved CRISPR/Cas9 gene editing by fluorescence activated cell sorting of green fluorescence protein tagged protoplasts.

    abstract:BACKGROUND:CRISPR/Cas9 is widely used for precise genetic editing in various organisms. CRISPR/Cas9 editing may in many plants be hampered by the presence of complex and high ploidy genomes and inefficient or poorly controlled delivery of the CRISPR/Cas9 components to gamete cells or cells with regenerative potential. ...

    journal_title:BMC biotechnology

    pub_type: 杂志文章

    doi:10.1186/s12896-019-0530-x

    authors: Petersen BL,Möller SR,Mravec J,Jørgensen B,Christensen M,Liu Y,Wandall HH,Bennett EP,Yang Z

    更新日期:2019-06-17 00:00:00

  • Identification of residues important for the activity of aldehyde-deformylating oxygenase through investigation into the structure-activity relationship.

    abstract:BACKGROUND:Aldehyde-deformylating oxygenase (ADO) is a key enzyme involved in the biosynthetic pathway of fatty alk(a/e)nes in cyanobacteria. However, cADO (cyanobacterial ADO) showed extreme low activity with the k cat value below 1 min-1, which would limit its application in biofuel production. To identify the activi...

    journal_title:BMC biotechnology

    pub_type: 杂志文章

    doi:10.1186/s12896-017-0351-8

    authors: Wang Q,Bao L,Jia C,Li M,Li JJ,Lu X

    更新日期:2017-03-16 00:00:00

  • Suitability of nutrients removal from brewery wastewater using a hydroponic technology with Typha latifolia.

    abstract:BACKGROUND:This study aims to assess suitability of hydroponic technology for treatment of brewery wastewater in a hydroponic bioreactor using Typha latifolia. Triplicated hydroponic bioreactor treatment units were designed, constructed and operated at a hydraulic retention time of 5 days with different surface loading...

    journal_title:BMC biotechnology

    pub_type: 杂志文章

    doi:10.1186/s12896-018-0484-4

    authors: Gebeyehu A,Shebeshe N,Kloos H,Belay S

    更新日期:2018-11-22 00:00:00

  • Biosynthesis of methylated resveratrol analogs through the construction of an artificial biosynthetic pathway in E. coli.

    abstract:BACKGROUND:Methylated resveratrol analogs show similar biological activities that are comparable with those of the resveratrol. However, the methylated resveratrol analogs exhibit better bioavailability as they are more easily transported into the cell and more resistant to degradation. Although these compounds are wid...

    journal_title:BMC biotechnology

    pub_type: 杂志文章

    doi:10.1186/1472-6750-14-67

    authors: Kang SY,Lee JK,Choi O,Kim CY,Jang JH,Hwang BY,Hong YS

    更新日期:2014-07-17 00:00:00

  • Recombinant nucleases CEL I from celery and SP I from spinach for mutation detection.

    abstract:BACKGROUND:The detection of unknown mutations is important in research and medicine. For this purpose, a mismatch-specific endonuclease CEL I from celery has been established as a useful tool in high throughput projects. Previously, CEL I-like activities were described only in a variety of plants and could not be expre...

    journal_title:BMC biotechnology

    pub_type: 杂志文章

    doi:10.1186/1472-6750-7-29

    authors: Pimkin M,Caretti E,Canutescu A,Yeung JB,Cohn H,Chen Y,Oleykowski C,Bellacosa A,Yeung AT

    更新日期:2007-06-01 00:00:00

  • A new chitinase-like xylanase inhibitor protein (XIP) from coffee (Coffea arabica) affects Soybean Asian rust (Phakopsora pachyrhizi) spore germination.

    abstract:BACKGROUND:Asian rust (Phakopsora pachyrhizi) is a common disease in Brazilian soybean fields and it is difficult to control. To identify a biochemical candidate with potential to combat this disease, a new chitinase-like xylanase inhibitor protein (XIP) from coffee (Coffea arabica) (CaclXIP) leaves was cloned into the...

    journal_title:BMC biotechnology

    pub_type: 杂志文章

    doi:10.1186/1472-6750-11-14

    authors: Vasconcelos EA,Santana CG,Godoy CV,Seixas CD,Silva MS,Moreira LR,Oliveira-Neto OB,Price D,Fitches E,Filho EX,Mehta A,Gatehouse JA,Grossi-De-Sa MF

    更新日期:2011-02-07 00:00:00

  • Ultrasound and microbubble induced release from intracellular compartments.

    abstract:BACKGROUND:Ultrasound and microbubbles (USMB) have been shown to enhance the intracellular uptake of molecules, generally thought to occur as a result of sonoporation. The underlying mechanism associated with USMB-enhanced intracellular uptake such as membrane disruption and endocytosis may also be associated with USMB...

    journal_title:BMC biotechnology

    pub_type: 杂志文章

    doi:10.1186/s12896-017-0364-3

    authors: Hussein F,Antonescu C,Karshafian R

    更新日期:2017-05-18 00:00:00

  • Determination of some significant batch culture conditions affecting acetyl-xylan esterase production by Penicillium notatum NRRL-1249.

    abstract:BACKGROUND:Acetyl-xylan esterase (AXE, EC 3.1.1.72) hydrolyses acetate group from the linear chain of xylopyranose residues bound by β-1,4-linkage. The enzyme finds commercial applications in bio-bleaching of wood pulp, treating animal feed to increase digestibility, processing food to increase clarification and conver...

    journal_title:BMC biotechnology

    pub_type: 杂志文章

    doi:10.1186/1472-6750-11-52

    authors: Atta S,Ali S,Akhtar MN,Haq I

    更新日期:2011-05-16 00:00:00

  • The trade-off of availability and growth inhibition through copper for the production of copper-dependent enzymes by Pichia pastoris.

    abstract:BACKGROUND:Copper is an essential chemical element for life as it is a part of prosthetic groups of enzymes including super oxide dismutase and cytochrome c oxidase; however, it is also toxic at high concentrations. Here, we present the trade-off of copper availability and growth inhibition of a common host used for co...

    journal_title:BMC biotechnology

    pub_type: 杂志文章

    doi:10.1186/s12896-016-0251-3

    authors: Balakumaran PA,Förster J,Zimmermann M,Charumathi J,Schmitz A,Czarnotta E,Lehnen M,Sudarsan S,Ebert BE,Blank LM,Meenakshisundaram S

    更新日期:2016-02-20 00:00:00

  • Keratinocyte cytoskeletal roles in cell sheet engineering.

    abstract:BACKGROUND:There is an increasing need to understand cell-cell interactions for cell and tissue engineering purposes, such as optimizing cell sheet constructs, as well as for examining adhesion defect diseases. For cell-sheet engineering, one major obstacle to sheet function is that cell sheets in suspension are fragil...

    journal_title:BMC biotechnology

    pub_type: 杂志文章

    doi:10.1186/1472-6750-13-17

    authors: Wei Q,Reidler D,Shen MY,Huang H

    更新日期:2013-02-26 00:00:00

  • Vaccination with recombinant Boophilus annulatus Bm86 ortholog protein, Ba86, protects cattle against B. annulatus and B. microplus infestations.

    abstract:BACKGROUND:The cattle ticks, Boophilus spp., affect cattle production in tropical and subtropical regions of the world. Tick vaccines constitute a cost-effective and environmentally friendly alternative to tick control. The recombinant B. microplus Bm86 protective antigen has been shown to protect cattle against tick i...

    journal_title:BMC biotechnology

    pub_type: 杂志文章

    doi:10.1186/1472-6750-9-29

    authors: Canales M,Almazán C,Naranjo V,Jongejan F,de la Fuente J

    更新日期:2009-03-31 00:00:00

  • Microporation is a valuable transfection method for efficient gene delivery into human umbilical cord blood-derived mesenchymal stem cells.

    abstract:BACKGROUND:Mesenchymal stem cells (MSCs) are an attractive source of adult stem cells for therapeutic application in clinical study. Genetic modification of MSCs with beneficial genes makes them more effective for therapeutic use. However, it is difficult to transduce genes into MSCs by common transfection methods, esp...

    journal_title:BMC biotechnology

    pub_type: 杂志文章

    doi:10.1186/1472-6750-10-38

    authors: Lim JY,Park SH,Jeong CH,Oh JH,Kim SM,Ryu CH,Park SA,Ahn JG,Oh W,Jeun SS,Chang JW

    更新日期:2010-05-13 00:00:00

  • A new single-step protocol for rapid baculovirus-driven protein production in insect cells.

    abstract:BACKGROUND:In the last three decades, the Baculovirus expression vector system (BEV) has evolved to one of the most widely used eukaryotic systems for heterologous protein expression including approved vaccines and therapies. Despite the significant improvements introduced during the past years, the BEV system still ha...

    journal_title:BMC biotechnology

    pub_type: 杂志文章

    doi:10.1186/s12896-017-0400-3

    authors: Scholz J,Suppmann S

    更新日期:2017-11-16 00:00:00

  • Total RNA extraction from tissues for microRNA and target gene expression analysis: not all kits are created equal.

    abstract:BACKGROUND:microRNAs (miRNAs) are short non-coding RNAs that fine-tune gene expression. The aberrant expression of miRNAs is associated with many diseases and they have both therapeutic and biomarker potential. However, our understanding of their usefulness is dependent on the tools we have to study them. Previous stud...

    journal_title:BMC biotechnology

    pub_type: 杂志文章

    doi:10.1186/s12896-018-0421-6

    authors: Brown RAM,Epis MR,Horsham JL,Kabir TD,Richardson KL,Leedman PJ

    更新日期:2018-03-16 00:00:00

  • Improved elongation factor-1 alpha-based vectors for stable high-level expression of heterologous proteins in Chinese hamster ovary cells.

    abstract:BACKGROUND:Establishing highly productive clonal cell lines with constant productivity over 2-3 months of continuous culture remains a tedious task requiring the screening of tens of thousands of clonal colonies. In addition, long-term cultivation of many candidate lines derived in the absence of drug selection pressur...

    journal_title:BMC biotechnology

    pub_type: 杂志文章

    doi:10.1186/1472-6750-14-56

    authors: Orlova NA,Kovnir SV,Hodak JA,Vorobiev II,Gabibov AG,Skryabin KG

    更新日期:2014-06-14 00:00:00

  • Different genome-wide transcriptome responses of Nocardioides simplex VKM Ac-2033D to phytosterol and cortisone 21-acetate.

    abstract:BACKGROUND:Bacterial degradation/transformation of steroids is widely investigated to create biotechnologically relevant strains for industrial application. The strain of Nocardioides simplex VKM Ac-2033D is well known mainly for its superior 3-ketosteroid Δ1-dehydrogenase activity towards various 3-oxosteroids and oth...

    journal_title:BMC biotechnology

    pub_type: 杂志文章

    doi:10.1186/s12896-021-00668-9

    authors: Shtratnikova VY,Sсhelkunov MI,Fokina VV,Bragin EY,Shutov AA,Donova MV

    更新日期:2021-01-13 00:00:00

  • Comparison of estimation capabilities of response surface methodology (RSM) with artificial neural network (ANN) in lipase-catalyzed synthesis of palm-based wax ester.

    abstract:BACKGROUND:Wax esters are important ingredients in cosmetics, pharmaceuticals, lubricants and other chemical industries due to their excellent wetting property. Since the naturally occurring wax esters are expensive and scarce, these esters can be produced by enzymatic alcoholysis of vegetable oils. In an enzymatic rea...

    journal_title:BMC biotechnology

    pub_type: 杂志文章

    doi:10.1186/1472-6750-7-53

    authors: Basri M,Rahman RN,Ebrahimpour A,Salleh AB,Gunawan ER,Rahman MB

    更新日期:2007-08-30 00:00:00