Abstract:
BACKGROUND:Microarrays are powerful tools for DNA-based molecular diagnostics and identification of pathogens. Most target a limited range of organisms and are based on only one or a very few genes for specific identification. Such microarrays are limited to organisms for which specific probes are available, and often have difficulty discriminating closely related taxa. We have developed an alternative broad-spectrum microarray that employs hybridisation fingerprints generated by high-density anonymous markers distributed over the entire genome for identification based on comparison to a reference database. RESULTS:A high-density microarray carrying 95,000 unique 13-mer probes was designed. Optimized methods were developed to deliver reproducible hybridisation patterns that enabled confident discrimination of bacteria at the species, subspecies, and strain levels. High correlation coefficients were achieved between replicates. A sub-selection of 12,071 probes, determined by ANOVA and class prediction analysis, enabled the discrimination of all samples in our panel. Mismatch probe hybridisation was observed but was found to have no effect on the discriminatory capacity of our system. CONCLUSIONS:These results indicate the potential of our genome chip for reliable identification of a wide range of bacterial taxa at the subspecies level without laborious prior sequencing and probe design. With its high resolution capacity, our proof-of-principle chip demonstrates great potential as a tool for molecular diagnostics of broad taxonomic groups.
journal_name
BMC Biotechnoljournal_title
BMC biotechnologyauthors
Pasquer F,Pelludat C,Duffy B,Frey JEdoi
10.1186/1472-6750-10-13subject
Has Abstractpub_date
2010-02-17 00:00:00pages
13issn
1472-6750pii
1472-6750-10-13journal_volume
10pub_type
杂志文章abstract:BACKGROUND:Eleven new human polyomaviruses (HPyVs) have been identified in the last decade. Serological studies show that these novel HPyVs sub-clinically infect humans at an early age. The routes of infection, entry pathways, and cell tropism of new HPyVs remain unknown. VP1 proteins of polyomaviruses can assembly int...
journal_title:BMC biotechnology
pub_type: 杂志文章
doi:10.1186/s12896-015-0187-z
更新日期:2015-08-04 00:00:00
abstract:BACKGROUND:There is an increasing need to understand cell-cell interactions for cell and tissue engineering purposes, such as optimizing cell sheet constructs, as well as for examining adhesion defect diseases. For cell-sheet engineering, one major obstacle to sheet function is that cell sheets in suspension are fragil...
journal_title:BMC biotechnology
pub_type: 杂志文章
doi:10.1186/1472-6750-13-17
更新日期:2013-02-26 00:00:00
abstract:BACKGROUND:Methylated resveratrol analogs show similar biological activities that are comparable with those of the resveratrol. However, the methylated resveratrol analogs exhibit better bioavailability as they are more easily transported into the cell and more resistant to degradation. Although these compounds are wid...
journal_title:BMC biotechnology
pub_type: 杂志文章
doi:10.1186/1472-6750-14-67
更新日期:2014-07-17 00:00:00
abstract:BACKGROUND:β-Fructofuranosidases (or invertases) catalyse the commercially-important biotransformation of sucrose into short-chain fructooligosaccharides with wide-scale application as a prebiotic in the functional foods and pharmaceutical industries. RESULTS:We identified a β-fructofuranosidase gene (CmINV) from a Ce...
journal_title:BMC biotechnology
pub_type: 杂志文章
doi:10.1186/1472-6750-13-100
更新日期:2013-11-14 00:00:00
abstract:BACKGROUND:The ability of cytosine deaminase (CD) to convert the antifungal agent 5-fluorocytosine (5-FC) into one of the most potent and largely used anticancer compound such as 5-fluorouracil (5-FU) raised considerable interest in this enzyme to model gene or antibody - directed enzyme-prodrug therapy (GDEPT/ADEPT) a...
journal_title:BMC biotechnology
pub_type: 杂志文章
doi:10.1186/1472-6750-8-68
更新日期:2008-09-10 00:00:00
abstract:BACKGROUND:Recombinant factor VIII (FVIII), used for haemophilia A therapy, is one of the most challenging among the therapeutic proteins produced in heterologous expression systems. Deletion variant of FVIII, in which the entire domain B is replaced by a short linker peptide, was approved for medical use. Efficacy and...
journal_title:BMC biotechnology
pub_type: 杂志文章
doi:10.1186/s12896-017-0353-6
更新日期:2017-03-24 00:00:00
abstract:BACKGROUND:Interest in using plants for production of recombinant proteins such as monoclonal antibodies is growing, but proteolytic degradation, leading to a loss of functionality and complications in downstream purification, is still a serious problem. RESULTS:In this study, we investigated the dynamics of the assem...
journal_title:BMC biotechnology
pub_type: 杂志文章
doi:10.1186/1472-6750-11-128
更新日期:2011-12-30 00:00:00
abstract:BACKGROUND:The identification of known mutations in a cell population is important for clinical applications and basic cancer research. In this work an immobilized form of the polymerase chain reaction, referred to as polony technology, was used to detect mutations as well as gene deletions, resulting in loss of hetero...
journal_title:BMC biotechnology
pub_type: 杂志文章
doi:10.1186/1472-6750-3-11
更新日期:2003-07-23 00:00:00
abstract:BACKGROUND:Human TGF-β3 has been used in many studies to induce genes coding for typical cartilage matrix components and accelerate chondrogenic differentiation, making it the standard constituent in most cultivation media used for the assessment of chondrogenesis associated with various stem cell types on carrier matr...
journal_title:BMC biotechnology
pub_type: 杂志文章
doi:10.1186/s12896-020-00641-y
更新日期:2020-08-27 00:00:00
abstract:BACKGROUND:Nitrilases attract increasing attention due to their utility in the mild hydrolysis of nitriles. According to activity and gene screening, filamentous fungi are a rich source of nitrilases distinct in evolution from their widely examined bacterial counterparts. However, fungal nitrilases have been less explo...
journal_title:BMC biotechnology
pub_type: 杂志文章,收录出版
doi:10.1186/1472-6750-11-2
更新日期:2011-01-06 00:00:00
abstract:BACKGROUND:Vascular endothelial growth factor (VEGF) is a key angiogenic factors. It plays an important role in both physiologic and pathologic angiogenesis and increases permeability across the vessels. Using antibody phage display technology, we obtained a novel anti-VEGFA IgG, named as FD006. In this study, the phar...
journal_title:BMC biotechnology
pub_type: 杂志文章
doi:10.1186/1472-6750-14-17
更新日期:2014-02-27 00:00:00
abstract:BACKGROUND:Taxol is an effective antineoplastic agent, originally extracted from the bark of Taxus brevifolia with a low yield. Many attempts have been made to produce Taxol by chemical synthesis, semi-synthesis and plant tissue cultures. However, to date, the availability of this compound is not sufficient to satisfy ...
journal_title:BMC biotechnology
pub_type: 杂志文章
doi:10.1186/1472-6750-6-45
更新日期:2006-12-06 00:00:00
abstract:BACKGROUND:Polysialic acid (polySia) is a carbohydrate modification of the neural cell adhesion molecule (NCAM), which is implicated in neural differentiation and plays an important role in tumor development and metastasis. Polysialylation of NCAM is mediated by two Golgi-resident polysialyltransferases (polyST) ST8Sia...
journal_title:BMC biotechnology
pub_type: 杂志文章
doi:10.1186/s12896-017-0360-7
更新日期:2017-05-12 00:00:00
abstract:BACKGROUND:Construction of high quality cDNA libraries from the usually low amounts of eukaryotic mRNA extracted from environmental samples is essential in functional metatranscriptomics for the selection of functional, full-length genes encoding proteins of interest. Many of the inserts in libraries constructed by sta...
journal_title:BMC biotechnology
pub_type: 杂志文章
doi:10.1186/1472-6750-14-80
更新日期:2014-09-03 00:00:00
abstract:BACKGROUND:Waste animal fat is a promising feedstock to replace vegetable oil that widely used in commercial biodiesel process, however the high content of free fatty acid in waste fat makes it unfeasible to be processed with commercial base-catalytic process. Enzymatic process is preferable to convert waste fat into b...
journal_title:BMC biotechnology
pub_type: 杂志文章
doi:10.1186/s12896-017-0390-1
更新日期:2017-09-09 00:00:00
abstract:BACKGROUND:Advances in optical imaging modalities and the continued evolution of genetically-encoded fluorescent proteins are coming together to facilitate the study of cell behavior at high resolution in living organisms. As a result, imaging using autofluorescent protein reporters is gaining popularity in mouse trans...
journal_title:BMC biotechnology
pub_type: 杂志文章
doi:10.1186/1472-6750-4-33
更新日期:2004-12-24 00:00:00
abstract:BACKGROUND:Thermally stable α-L-rhamnosidase with cleaving terminal α-L-rhamnose activity has great potential in industrial application. Therefore, it is necessary to find a proper method to improve the thermal stability of α-L-rhamnosidase. RESULTS:In this study, addition of sorbitol has been found to increase the th...
journal_title:BMC biotechnology
pub_type: 杂志文章
doi:10.1186/s12896-017-0342-9
更新日期:2017-02-27 00:00:00
abstract:BACKGROUND:Insect resistance in crops represents a main challenge for agriculture. Transgenic approaches based on proteins displaying insect resistance properties are widely used as efficient breeding strategies. To extend the spectrum of targeted pathogens and overtake the development of resistance, molecular evolutio...
journal_title:BMC biotechnology
pub_type: 杂志文章
doi:10.1186/s12896-019-0507-9
更新日期:2019-03-08 00:00:00
abstract:BACKGROUND:L-ornithine is effective in the treatment of liver diseases and helps strengthen the heart. The commercial applications mean that efficient biotechnological production of L-ornithine has become increasingly necessary. Adaptive evolution strategies have been proven a feasible and efficient technique to achiev...
journal_title:BMC biotechnology
pub_type: 杂志文章
doi:10.1186/1472-6750-13-47
更新日期:2013-06-01 00:00:00
abstract:BACKGROUND:Baculovirus, which has a width of 40 nm and a length of 250-300 nm, can display functional peptides, receptors and antigens on its surface by their fusion with a baculovirus envelop protein, GP64. In addition, some transmembrane proteins can be displayed without GP64 fusion, using the native transmembrane do...
journal_title:BMC biotechnology
pub_type: 杂志文章
doi:10.1186/1472-6750-11-60
更新日期:2011-06-02 00:00:00
abstract:BACKGROUND:Ultrasound and microbubbles (USMB) have been shown to enhance the intracellular uptake of molecules, generally thought to occur as a result of sonoporation. The underlying mechanism associated with USMB-enhanced intracellular uptake such as membrane disruption and endocytosis may also be associated with USMB...
journal_title:BMC biotechnology
pub_type: 杂志文章
doi:10.1186/s12896-017-0364-3
更新日期:2017-05-18 00:00:00
abstract:BACKGROUND:Rapid, robust and reversible induction of transgene expression would significantly facilitate cancer gene therapy as well as allow the in vivo functional study of newly discovered genes in tumor formation and progression. The popularity of the ecdysone inducible gene switch system has led us to investigate w...
journal_title:BMC biotechnology
pub_type: 杂志文章
doi:10.1186/1472-6750-1-11
更新日期:2001-01-01 00:00:00
abstract:BACKGROUND:Increasing cell membrane permeability to water and cryoprotectants is critical for the successful cryopreservation of cells with large volumes. Artificial expression of water-selective aquaporins or aquaglyceroporins (GLPs), such as mammalian aquaporin-3 (AQP3), enhances cell permeability to water and cryopr...
journal_title:BMC biotechnology
pub_type: 杂志文章
doi:10.1186/1472-6750-11-34
更新日期:2011-04-08 00:00:00
abstract:BACKGROUND:The CRISPR-Cas9 system is a powerful and versatile tool for crop genome editing. However, achieving highly efficient and specific editing in polyploid species can be a challenge. The efficiency and specificity of the CRISPR-Cas9 system depends critically on the gRNA used. Here, we assessed the activities and...
journal_title:BMC biotechnology
pub_type: 杂志文章
doi:10.1186/s12896-019-0565-z
更新日期:2019-10-30 00:00:00
abstract:: ...
journal_title:BMC biotechnology
pub_type: 杂志文章
doi:10.1186/s12896-019-0504-z
更新日期:2019-02-18 00:00:00
abstract:BACKGROUND:In view of compliance with increasingly stringent environmental legislation, an eco-friendly treatment technology of industrial dyes and effluents is a major environmental challenge in the color industry. In present study, a promising and eco-friendly entrapment approach was adopted to immobilize purified ma...
journal_title:BMC biotechnology
pub_type: 杂志文章
doi:10.1186/s12896-015-0227-8
更新日期:2015-12-10 00:00:00
abstract:BACKGROUND:Nanoparticles appear to be promising devices for application in the agriculture and food industries, but information regarding the response of plants to contact with nano-devices is scarce. Toxic effects may be imposed depending on the type and concentration of nanoparticle as well as time of exposure. A num...
journal_title:BMC biotechnology
pub_type: 杂志文章
doi:10.1186/1472-6750-13-111
更新日期:2013-12-20 00:00:00
abstract:BACKGROUND:Carboxyl-terminal protease (CtpA) plays essential functions in posttranslational protein processing in prokaryotic and eukaryotic cells. To date, only a few bacterial ctpA genes have been characterized. Here we cloned and characterized a novel CtpA. The encoding gene, ctpAp (ctpA of Paenibacillus lautus), wa...
journal_title:BMC biotechnology
pub_type: 杂志文章
doi:10.1186/1472-6750-13-89
更新日期:2013-10-25 00:00:00
abstract:BACKGROUND:MicroRNAs are important regulators of gene expression at the post-transcriptional level and play an important role in many biological processes. Due to the important biological role it is of great interest to quantitatively determine their expression level in different biological settings. RESULTS:We descri...
journal_title:BMC biotechnology
pub_type: 杂志文章
doi:10.1186/1472-6750-11-70
更新日期:2011-06-25 00:00:00
abstract:BACKGROUND:There are significant differences in the culture conditions between small-scale screenings and large-scale fermentation processes. Production processes are usually conducted in fed-batch cultivation mode with active pH-monitoring and control. In contrast, screening experiments in shake flasks are usually con...
journal_title:BMC biotechnology
pub_type: 杂志文章
doi:10.1186/1472-6750-11-25
更新日期:2011-03-23 00:00:00