Abstract:
:The tumour microenvironment may contribute to tumorigenesis owing to mechanical forces such as fibrotic stiffness or mechanical pressure caused by the expansion of hyper-proliferative cells. Here we explore the contribution of the mechanical pressure exerted by tumour growth onto non-tumorous adjacent epithelium. In the early stage of mouse colon tumour development in the Notch(+)Apc(+/1638N) mouse model, we observed mechanistic pressure stress in the non-tumorous epithelial cells caused by hyper-proliferative adjacent crypts overexpressing active Notch, which is associated with increased Ret and β-catenin signalling. We thus developed a method that allows the delivery of a defined mechanical pressure in vivo, by subcutaneously inserting a magnet close to the mouse colon. The implanted magnet generated a magnetic force on ultra-magnetic liposomes, stabilized in the mesenchymal cells of the connective tissue surrounding colonic crypts after intravenous injection. The magnetically induced pressure quantitatively mimicked the endogenous early tumour growth stress in the order of 1,200 Pa, without affecting tissue stiffness, as monitored by ultrasound strain imaging and shear wave elastography. The exertion of pressure mimicking that of tumour growth led to rapid Ret activation and downstream phosphorylation of β-catenin on Tyr654, imparing its interaction with the E-cadherin in adherens junctions, and which was followed by β-catenin nuclear translocation after 15 days. As a consequence, increased expression of β-catenin-target genes was observed at 1 month, together with crypt enlargement accompanying the formation of early tumorous aberrant crypt foci. Mechanical activation of the tumorigenic β-catenin pathway suggests unexplored modes of tumour propagation based on mechanical signalling pathways in healthy epithelial cells surrounding the tumour, which may contribute to tumour heterogeneity.
journal_name
Naturejournal_title
Natureauthors
Fernández-Sánchez ME,Barbier S,Whitehead J,Béalle G,Michel A,Latorre-Ossa H,Rey C,Fouassier L,Claperon A,Brullé L,Girard E,Servant N,Rio-Frio T,Marie H,Lesieur S,Housset C,Gennisson JL,Tanter M,Ménager C,Fre S,Robdoi
10.1038/nature14329subject
Has Abstractpub_date
2015-07-02 00:00:00pages
92-5issue
7558eissn
0028-0836issn
1476-4687pii
nature14329journal_volume
523pub_type
杂志文章相关文献
NATURE文献大全abstract::The ability of cells to form cell contacts, adhere to the extracellular matrix, change morphology, and migrate is essential for development, wound healing, metastasis, cell survival and the immune response. These events depend on the binding of integrin to the extracellular matrix, and assembly of focal adhesions, whi...
journal_title:Nature
pub_type: 杂志文章
doi:10.1038/nature01082
更新日期:2002-11-07 00:00:00
abstract::B lymphocytes re-circulate between B-cell-rich compartments (follicles or B zones) in secondary lymphoid organs, surveying for antigen. After antigen binding, B cells move to the boundary of B and T zones to interact with T-helper cells. Despite the importance of B--T-cell interactions for the induction of antibody re...
journal_title:Nature
pub_type: 杂志文章
doi:10.1038/416094a
更新日期:2002-03-07 00:00:00
abstract::Piezoelectric materials, which convert mechanical to electrical energy (and vice versa), are crucial in medical imaging, telecommunication and ultrasonic devices. A new generation of single-crystal materials, such as Pb(Zn1/3Nb2/3)O3-PbTiO3 (PZN-PT) and Pb(Mg1/3Nb2/3)O3-PbTiO3 (PMN-PT), exhibit a piezoelectric effect ...
journal_title:Nature
pub_type: 杂志文章
doi:10.1038/35002022
更新日期:2000-01-20 00:00:00
abstract::An inductor, one of the most fundamental circuit elements in modern electronic devices, generates a voltage proportional to the time derivative of the input current1. Conventional inductors typically consist of a helical coil and induce a voltage as a counteraction to time-varying magnetic flux penetrating the coil, f...
journal_title:Nature
pub_type: 杂志文章
doi:10.1038/s41586-020-2775-x
更新日期:2020-10-01 00:00:00
abstract::Secretory-protein translocation into the endoplasmic reticulum (ER) is thought to be catalysed by integral membrane proteins. Genetic selections uncovered three Saccharomyces cerevisiae genes (SEC61, SEC62 and SEC63), mutations in which block import of precursor proteins into the ER lumen in vivo and in vitro. The DNA...
journal_title:Nature
pub_type: 杂志文章
doi:10.1038/349806a0
更新日期:1991-02-28 00:00:00
abstract::Megakaryocytopoiesis is the cellular developmental process that leads to platelet production. At least two humoral growth factors may be necessary for megakaryocyte proliferation and maturation. One is a megakaryocyte-colony stimulating factor (MK-CSF) which induces the proliferation and differentiation of megakaryocy...
journal_title:Nature
pub_type: 杂志文章
doi:10.1038/369571a0
更新日期:1994-06-16 00:00:00
abstract::What is it that defines an animal? The definition provided here, made on the basis of developmental biology, suggests methods for resolving phylogenetic problems. ...
journal_title:Nature
pub_type: 杂志文章
doi:10.1038/361490a0
更新日期:1993-02-11 00:00:00
abstract::The dry valleys of southern Victoria Land in Antarctica contain several closed basins in which perennially ice-covered lakes are found. One of the most unusual features of these lakes is the occurrence of high O2 concentrations in the water column; values ranging from slightly more than saturation to more than four t...
journal_title:Nature
pub_type: 杂志文章
doi:10.1038/325343a0
更新日期:1987-01-22 00:00:00
abstract::Apoptosis is a highly regulated process that is crucial for normal development and homeostasis of multicellular organisms. The p35 protein from baculoviruses effectively prevents apoptosis by its broad-spectrum caspase inhibition. Here we report the crystal structure of p35 in complex with human caspase-8 at 3.0 A res...
journal_title:Nature
pub_type: 杂志文章
doi:10.1038/35068604
更新日期:2001-03-22 00:00:00
abstract::During cell division, chromosomes are segregated to nascent daughter cells by attaching to the microtubules of the mitotic spindle through the kinetochore. Kinetochores are assembled on a specialized chromatin domain called the centromere, which is characterized by the replacement of nucleosomal histone H3 with the hi...
journal_title:Nature
pub_type: 杂志文章
doi:10.1038/nature10379
更新日期:2011-08-28 00:00:00
abstract::The spectral content of skin vibrations, produced by either displacing the finger across a surface texture1 or passively sensing external movements through the solid substrate2,3, provides fundamental information about our environment. Low-frequency flutter (below 50 Hz) applied locally to the primate fingertip evokes...
journal_title:Nature
pub_type: 杂志文章
doi:10.1038/s41586-019-1015-8
更新日期:2019-03-01 00:00:00
abstract::Optical laser fields have been widely used to achieve quantum control over the motional and internal degrees of freedom of atoms and ions, molecules and atomic gases. A route to controlling the quantum states of macroscopic mechanical oscillators in a similar fashion is to exploit the parametric coupling between optic...
journal_title:Nature
pub_type: 杂志文章
doi:10.1038/nature10787
更新日期:2012-02-01 00:00:00
abstract::A congressional investigation alleges that some researchers have failed to report all the drug-company money that they have received--and that universities may have been too slow to police them. ...
journal_title:Nature
pub_type: 社论
doi:10.1038/455835a
更新日期:2008-10-16 00:00:00
abstract::Globular star clusters are compact and massive stellar systems old enough to have witnessed the entire history of our Galaxy, the Milky Way. Although recent results suggest that their formation may have been more complex than previously thought, they still are the best approximation to a stellar population formed over...
journal_title:Nature
pub_type: 杂志文章
doi:10.1038/nature08581
更新日期:2009-11-26 00:00:00
abstract::It has been speculated that brain activities might directly control adaptive immune responses in lymphoid organs, although there is little evidence for this. Here we show that splenic denervation in mice specifically compromises the formation of plasma cells during a T cell-dependent but not T cell-independent immune ...
journal_title:Nature
pub_type: 杂志文章
doi:10.1038/s41586-020-2235-7
更新日期:2020-05-01 00:00:00
abstract::A long-standing goal of artificial intelligence is an algorithm that learns, tabula rasa, superhuman proficiency in challenging domains. Recently, AlphaGo became the first program to defeat a world champion in the game of Go. The tree search in AlphaGo evaluated positions and selected moves using deep neural networks....
journal_title:Nature
pub_type: 杂志文章
doi:10.1038/nature24270
更新日期:2017-10-18 00:00:00
abstract::Overexploitation of renewable resources today has a high cost on the welfare of future generations. Unlike in other public goods games, however, future generations cannot reciprocate actions made today. What mechanisms can maintain cooperation with the future? To answer this question, we devise a new experimental para...
journal_title:Nature
pub_type: 杂志文章
doi:10.1038/nature13530
更新日期:2014-07-10 00:00:00
abstract::We describe here a new view of primary visual cortex (V1) based on measurements of neural responses in V1 to patterns called 'illusory contours' (Fig. 1a, b). Detection of an object's boundary contours is a fundamental visual task. Boundary contours are defined by discontinuities not only in luminance and colour, but ...
journal_title:Nature
pub_type: 杂志文章
doi:10.1038/365550a0
更新日期:1993-10-07 00:00:00
abstract::The spider mite Tetranychus urticae is a cosmopolitan agricultural pest with an extensive host plant range and an extreme record of pesticide resistance. Here we present the completely sequenced and annotated spider mite genome, representing the first complete chelicerate genome. At 90 megabases T. urticae has the sma...
journal_title:Nature
pub_type: 杂志文章
doi:10.1038/nature10640
更新日期:2011-11-23 00:00:00
abstract::The use of viscid silk in aerial webs as a means to capture prey was a key innovation of araneoid spiders and has contributed largely to their ecological success. Here I describe a single silk thread from a spider's web that bears glue droplets and has been preserved in Lebanese amber from the Early Cretaceous period ...
journal_title:Nature
pub_type: 杂志文章
doi:10.1038/424636a
更新日期:2003-08-07 00:00:00
abstract::On the basis of their primary structure, the lysine-rich histones are a unified family of proteins. Each has an amino acid chain which falls into three distinct domains. Only the central domain (approximately 80 residues) is in a folded conformation. It is protected from trypsin digestion in chromatin and corresponds ...
journal_title:Nature
pub_type: 杂志文章
doi:10.1038/288675a0
更新日期:1980-12-25 00:00:00
abstract::The vertical structure of the recent Arctic warming contains information about the processes governing Arctic climate trends. Graversen et al. argue, on the basis of ERA-40 reanalysis data, that a distinct maximum in 1979-2001 warm-season (April-October) Arctic temperature trends appears around 3 km above ground. Here...
journal_title:Nature
pub_type: 评论,杂志文章
doi:10.1038/nature07257
更新日期:2008-09-11 00:00:00
abstract::The rat hippocampus receives a strong cholinergic innervation from the medial septum; information about the development and function of this pathway could help to elucidate the mechanisms of memory functions. Previous electrophysiological studies have shown that septal stimulation in vivo facilitates commissural and p...
journal_title:Nature
pub_type: 杂志文章
doi:10.1038/313577a0
更新日期:1985-02-14 00:00:00
abstract::Electrical current can be completely spin polarized in a class of materials known as half-metals, as a result of the coexistence of metallic nature for electrons with one spin orientation and insulating nature for electrons with the other. Such asymmetric electronic states for the different spins have been predicted f...
journal_title:Nature
pub_type: 杂志文章
doi:10.1038/nature05180
更新日期:2006-11-16 00:00:00
abstract::DNA methylation is an important epigenetic modification that is essential for various developmental processes through regulating gene expression, genomic imprinting, and epigenetic inheritance. Mammalian genomic DNA methylation is established during embryogenesis by de novo DNA methyltransferases, DNMT3A and DNMT3B, a...
journal_title:Nature
pub_type: 杂志文章
doi:10.1038/nature13899
更新日期:2015-01-29 00:00:00
abstract:: ...
journal_title:Nature
pub_type: 社论
doi:10.1038/d41586-018-07033-5
更新日期:2018-10-01 00:00:00
abstract::A thermal light-emitting source, such as a black body or the incandescent filament of a light bulb, is often presented as a typical example of an incoherent source and is in marked contrast to a laser. Whereas a laser is highly monochromatic and very directional, a thermal source has a broad spectrum and is usually qu...
journal_title:Nature
pub_type: 杂志文章
doi:10.1038/416061a
更新日期:2002-03-07 00:00:00
abstract::Many planktonic organisms produce 'resting' stages when the environmental conditions deteriorate. Like seeds, resting stages can survive unfavourable conditions. The crustacean Daphnia normally reproduces by means of parthenogenetically produced normal, not resting, eggs-but occasionally switches to bisexual reproduct...
journal_title:Nature
pub_type: 杂志文章
doi:10.1038/414899a
更新日期:2001-12-20 00:00:00
abstract::Because it is so tall, the giraffe, Giraffa camelopardalis, provides an important animal model for investigating adaptive mechanisms to orthostatic (gravitational) pressure changes. Previous physiological studies of the giraffe have concentrated on arterial blood pressures in the heart and neck. Briefly, these investi...
journal_title:Nature
pub_type: 杂志文章
doi:10.1038/329059a0
更新日期:1987-09-03 00:00:00
abstract::It is generally thought that interspecific (lateral) transfer of genes is so extensive among bacteria that it is difficult, and perhaps impossible, to determine their phylogenetic relationships. Ambler and coworkers reflect this in their suggestion that the relationships seen among cytochrome c sequences of the Rhodos...
journal_title:Nature
pub_type: 杂志文章
doi:10.1038/283212a0
更新日期:1980-01-10 00:00:00