Co-localization of tyrosine hydroxylase and GABA immunoreactivities in human cortical neurons.

Abstract:

:Samples of human cerebral cortex were stained immunocytochemically for tyrosine hydroxylase (TH) and gamma-aminobutyric acid (GABA). TH-positive neurons were in small number and predominated in the deep infragranular layers V-VI contrasting with numerous GABA-positive neurons scattered in all layers. Co-localization of TH- and GABA-like immunoreactivities in a single cell was studied by the double immunolabeling technique with the elution-restaining procedure. Only 50% of the TH-positive neurons also expressed GABA-like immunoreactivities. The two markers were detectable in the somata and not in the processes of the cells. The double-labeled cells were mainly fusiform and medium-sized and were observed in layer VI. These observations suggest that the TH-positive cells form a mixed neuronal population, only a part of which corresponds to the GABAergic class of intrinsic interneurons.

journal_name

Neurosci Lett

journal_title

Neuroscience letters

authors

Trottier S,Geffard M,Evrard B

doi

10.1016/0304-3940(89)90205-x

subject

Has Abstract

pub_date

1989-11-20 00:00:00

pages

76-82

issue

1-2

eissn

0304-3940

issn

1872-7972

pii

0304-3940(89)90205-X

journal_volume

106

pub_type

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