Bioassay-guided isolation of antibacterial constituents from Diospyros lotus roots.

Abstract:

:The aim of this study was to explore the extract/fractions and compounds of Diospyros lotus against various Gram-positive and Gram-negative bacteria strain. The results showed marked susceptibility of extract and its fractions against test pathogens. Among them, chloroform fraction was most dominant and effective against all tested bacteria. The chloroform fraction was subjected to column chromatography which led to the isolation of lupeol (1), 7-methyljuglone (2), β-sitosterol (3), stigmasterol (4), betulinic acid (5), diospyrin (6) and 8-hydroxyisodiospyrin (7). Among the isolated compounds, betulinic acid (5) showed significant activity against most of the tested pathogen. In conclusion, our study validated the traditional uses of the plant in the treatment of infectious diseases which was also strongly supported by the isolated compound, betulinic acid (5).

journal_name

Nat Prod Res

journal_title

Natural product research

authors

Rauf A,Uddin G,Khan H,Arfan M,Siddiqui BS

doi

10.1080/14786419.2015.1013957

subject

Has Abstract

pub_date

2016-01-01 00:00:00

pages

426-8

issue

4

eissn

1478-6419

issn

1478-6427

journal_volume

30

pub_type

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