Abstract:
:In contrast to mechanisms mediating uptake of intracellular bacterial pathogens, bacterial egress and cell-to-cell transmission are poorly understood. Previously, we showed that the transmission of pathogenic mycobacteria between phagocytic cells also depends on nonlytic ejection through an F-actin based structure, called the ejectosome. How the host cell maintains integrity of its plasma membrane during the ejection process was unknown. Here, we reveal an unexpected function for the autophagic machinery in nonlytic spreading of bacteria. We show that ejecting mycobacteria are escorted by a distinct polar autophagocytic vacuole. If autophagy is impaired, cell-to-cell transmission is inhibited, the host plasma membrane becomes compromised and the host cells die. These findings highlight a previously unidentified, highly ordered interaction between bacteria and the autophagic pathway and might represent the ancient way to ensure nonlytic egress of bacteria.
journal_name
Proc Natl Acad Sci U S Aauthors
Gerstenmaier L,Pilla R,Herrmann L,Herrmann H,Prado M,Villafano GJ,Kolonko M,Reimer R,Soldati T,King JS,Hagedorn Mdoi
10.1073/pnas.1423318112subject
Has Abstractpub_date
2015-02-17 00:00:00pages
E687-92issue
7eissn
0027-8424issn
1091-6490pii
1423318112journal_volume
112pub_type
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journal_title:Proceedings of the National Academy of Sciences of the United States of America
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