当前位置： SCI文献检索 > MOLECULAR AND CELLULAR ENDOCRINOLOGY期刊下所有文献 > The anti-diabetic drug metformin inhibits vascular endothelial growth factor expression via the mammalian target of rapamycin complex 1/hypoxia-inducible factor-1α signaling pathway in ELT-3 cells.

The anti-diabetic drug metformin inhibits vascular endothelial growth factor expression via the mammalian target of rapamycin complex 1/hypoxia-inducible factor-1α signaling pathway in ELT-3 cells.

Abstract：

:The aim of this study was to elucidate whether metformin can regulate the expression of vascular endothelial growth factor (VEGF) in rat-derived uterine leiomyoma cells (ELT-3 cells). In vitro studies were conducted using ELT-3 cells. Under normoxic conditions, metformin suppressed VEGF protein levels in the supernatant and cells in a dose-dependent manner. In hypoxia-mimicking conditions, VEGF and hypoxia-inducible factor-1α (HIF-1α) proteins were both highly expressed and were suppressed by the metformin treatment. Metformin did not affect HIF-1α mRNA levels, which indicated that its effects occurred at the post-translational level. Metformin inhibited mammalian target of rapamycin complex 1 (mTORC1) activity by phosphorylating the mTORC1 component raptor. This study revealed the anti-angiogenic activity of metformin in ELT-3 cells by suppressing the expression of VEGF via the mTORC1/HIF-1α pathway. These results indicate that metformin may represent an effective alternative in the future treatment of uterine leiomyomas.

journal_name

Mol Cell Endocrinol

journal_title

Molecular and cellular endocrinology

authors

Tadakawa M,Takeda T,Li B,Tsuiji K,Yaegashi N

doi

10.1016/j.mce.2014.08.012

subject

Has Abstract

pub_date

2015-01-05 00:00:00

pages

1-8

eissn

0303-7207

issn

1872-8057

pii

S0303-7207(14)00269-X

journal_volume

399

pub_type

杂志文章

相关文献

MOLECULAR AND CELLULAR ENDOCRINOLOGY文献大全
  • Lactocrine programming of female reproductive tract development: environmental connections to the reproductive continuum.

    abstract：:For eutherian mammals a continuum of maternal support insures that development of progeny follows an optimal program. Beginning in utero, such support extends into the early neonatal period when bioactive factors are communicated from mother to offspring in colostrum/milk. Defined as lactocrine signaling, communicatio...

    journal_title：Molecular and cellular endocrinology

    pub_type： 杂志文章,评审

    doi：10.1016/j.mce.2011.10.008

    authors： Bartol FF,Bagnell CA

    更新日期：2012-05-06 00:00:00

  • Effect of thyroid status on the development of the different molecular forms of Na+,K+-ATPase in rat brain.

    abstract：:The effect of thyroid status on the postnatal development of the two molecular forms of Na+,K+-ATPase, distinguished kinetically on the basis of their ouabain sensitivity, was examined in rat brain. Hypothyroidism induced by PTU from day 1 postnatally significantly reduced the Na+,K+-ATPase activity in cerebellum (22-...

    journal_title：Molecular and cellular endocrinology

    pub_type： 杂志文章

    doi：10.1016/0303-7207(85)90170-4

    authors： Atterwill CK,Reid J,Athayde CM

    更新日期：1985-05-01 00:00:00

  • Oestrogen receptor in mammary gland cytosol of virgin, pregnant and lactating mice.

    abstract：:A macromolecular component binding 3H-labelled 17 beta-oestradiol in a specific manner and sedimenting in the 8-10-S region on sucrose gradient has been detected in the mammary gland cytosol of ovariectomized adult virgin mice. The dissociation constant of the macromolecule-oestradiol complex is 4.2 times 10(-10)M at ...

    journal_title：Molecular and cellular endocrinology

    pub_type： 杂志文章

    doi：10.1016/0303-7207(76)90007-1

    authors： Auricchio F,Rotondi A,Bresciani F

    更新日期：1975-12-01 00:00:00

  • Regulation of the biosynthesis of cytochromes P-450 involved in steroid hormone synthesis.

    abstract：:The actions of ACTH to regulate the synthesis of the various enzymes involved in steroid hormone biosynthesis have been studied using bovine adrenocortical cells in monolayer culture. ACTH causes an increase in the synthesis of both the mitochondrial and the microsomal forms of cytochrome P-450 involved in steroid hor...

    journal_title：Molecular and cellular endocrinology

    pub_type： 杂志文章,评审

    doi：10.1016/0303-7207(85)90123-6

    authors： Waterman MR,Simpson ER

    更新日期：1985-02-01 00:00:00

  • Cyclic nucleotide phosphodiesterase in developing rat testis. Identification of somatic and germ-cell forms.

    abstract：:Several forms of phosphodiesterase are present in the male gonad, and their relative concentrations vary during testicular development. On the basis of kinetic analysis and chromatography on DEAE cellulose, 3 forms were separated: (a) a high-affinity cGMP phosphodiesterase, regulated by Ca2+ and calmodulin, similar to...

    journal_title：Molecular and cellular endocrinology

    pub_type： 杂志文章

    doi：10.1016/0303-7207(82)90039-9

    authors： Geremia R,Rossi P,Pezzotti R,Conti M

    更新日期：1982-09-01 00:00:00

  • Regulation of corepressor alternative mRNA splicing by hormonal and metabolic signaling.

    abstract：:Alternative mRNA splicing diversifies the products encoded by the NCoR and SMRT corepressor loci. There is a programmed alteration in NCoR mRNA splicing during adipocyte differentiation from an NCoRω isoform, which contains three nuclear receptor interaction domains, to an NCoRδ isoform that contains two nuclear recep...

    journal_title：Molecular and cellular endocrinology

    pub_type： 杂志文章

    doi：10.1016/j.mce.2015.06.036

    authors： Snyder CA,Goodson ML,Schroeder AC,Privalsky ML

    更新日期：2015-09-15 00:00:00

  • Administration of growth hormone modulates the gene expression of basic fibroblast growth factor in rat costal cartilage, both in vivo and in vitro.

    abstract：:We examined the effect of growth hormone on local growth factor mRNA expression in male Sprague-Dawley rats. Repetitive systemic administration of growth hormone (0.4 IU every 4 h) increased the expression of IGF-I mRNA up to 2.8-fold in costal cartilage tissue compared with controls. Basic FGF (bFGF) mRNA expression ...

    journal_title：Molecular and cellular endocrinology

    pub_type： 杂志文章

    doi：10.1016/0303-7207(95)03591-t

    authors： Izumi T,Shida J,Jingushi S,Hotokebuchi T,Sugioka Y

    更新日期：1995-07-01 00:00:00

  • The spatial and temporal regulation of the hormonal signal. Role of mitochondria in the formation of a protein complex required for the activation of cholesterol transport and steroids synthesis.

    abstract：:The mitochondria are critical for steroidogenesis since the ability of cholesterol to move into mitochondria to be available for cytochrome P450, CYP11A1, determines the efficacy of steroid production. Several proteins kinases, such as PKA, MEK and ERK which are essential to complete steroidogenesis, form a mitochondr...

    journal_title：Molecular and cellular endocrinology

    pub_type： 杂志文章,评审

    doi：10.1016/j.mce.2012.12.024

    authors： Poderoso C,Duarte A,Cooke M,Orlando U,Gottifredi V,Solano AR,Lemos JR,Podestá EJ

    更新日期：2013-05-22 00:00:00

  • Estradiol-independent modulation of breast cancer transcript profile by 17beta-hydroxysteroid dehydrogenase type 1.

    abstract：:17beta-hydroxysteroid dehydrogenase type 1 (17β-HSD1) is a steroidal enzyme which, in breast cancer cells, mainly synthesizes 17-beta-estradiol (E2), an estrogenic hormone that stimulates breast cancer cell growth. We previously showed that the enzyme increased breast cancer cell proliferation via a dual effect on E2 ...

    journal_title：Molecular and cellular endocrinology

    pub_type： 杂志文章

    doi：10.1016/j.mce.2016.08.026

    authors： Aka JA,Calvo EL,Lin SX

    更新日期：2017-01-05 00:00:00

  • Effect of estetrol, a selective nuclear estrogen receptor modulator, in mouse models of arterial and venous thrombosis.

    abstract：:Estetrol (E4) is a natural estrogen synthesized exclusively during pregnancy by the human fetal liver, and the physiological role of this hormone is unknown. Interestingly, E4 was recently evaluated in preclinical and phase II-III clinical studies in combination with a progestin, with the advantage to not increase the...

    journal_title：Molecular and cellular endocrinology

    pub_type： 杂志文章

    doi：10.1016/j.mce.2018.06.010

    authors： Valéra MC,Noirrit-Esclassan E,Dupuis M,Fontaine C,Lenfant F,Briaux A,Cabou C,Garcia C,Lairez O,Foidart JM,Payrastre B,Arnal JF

    更新日期：2018-12-05 00:00:00

  • Valproic acid enhances Oct4 promoter activity through PI3K/Akt/mTOR pathway activated nuclear receptors.

    abstract：:Valproic acid (VPA) has been shown to increase the reprogramming efficiency of induced pluripotent stem cells (iPSC) from somatic cells, but the mechanism by which VPA enhances iPSC induction has not been defined. Here we demonstrated that VPA directly activated Oct4 promoter activity through activation of the PI3K/Ak...

    journal_title：Molecular and cellular endocrinology

    pub_type： 杂志文章

    doi：10.1016/j.mce.2013.12.008

    authors： Teng HF,Li PN,Hou DR,Liu SW,Lin CT,Loo MR,Kao CH,Lin KH,Chen SL

    更新日期：2014-03-05 00:00:00

  • Aromatase expression of human osteoblast-like cells.

    abstract：:Estrogen plays a major role in bone mineral homeostasis, maintaining a balance between bone formation and bone resorption not only in women but also in men. Extraglandular aromatization of circulating androgen is the major source of estrogen in post-menopausal women and men. In order to assess the capacity of bone cel...

    journal_title：Molecular and cellular endocrinology

    pub_type： 杂志文章

    doi：10.1016/s0303-7207(98)00069-0

    authors： Shozu M,Simpson ER

    更新日期：1998-04-30 00:00:00

  • Immunocytochemical localization of prolactin receptors in rat liver cells: I. Dependence on sex and sex steroids.

    abstract：:The peculiarities of cellular and tissue distribution of prolactin receptors (PRLR) in the liver of female and male rats with different sex steroid status were investigated in paraplast-embedded tissue with the indirect immunoperoxidase technique. Two clones of antibodies directed outside the PRL-binding site (U6) or ...

    journal_title：Molecular and cellular endocrinology

    pub_type： 杂志文章

    doi：10.1016/0303-7207(94)90037-x

    authors： Smirnova OV,Petraschuk OM,Kelly PA

    更新日期：1994-10-01 00:00:00

  • Aromatase, estrone sulfatase, and 17β-hydroxysteroid dehydrogenase: structure-function studies and inhibitor development.

    abstract：:Aromatase, estrone sulfatase, and 17β-hydroxysteroid dehydrogenase type 1 are involved in the key steps of 17β-estradiol biosynthesis. Structure-function studies of aromatase, estrone sulfatase and 17β-hydroxysteroid dehydrogenase type 1 are important to evaluate the molecular basis of the interaction between these en...

    journal_title：Molecular and cellular endocrinology

    pub_type： 杂志文章,评审

    doi：10.1016/j.mce.2010.09.012

    authors： Hong Y,Chen S

    更新日期：2011-07-04 00:00:00

  • Insulin effects on human red blood cells.

    abstract：:The effect of insulin on human red blood cells was investigated, both on intact cells and on isolated plasma membranes, testing the responsiveness of membrane-bound enzymes--such as (Na+-K+)-ATPase and 5'-nucleotidase--as well as the ouabain binding and ionic fluxes. It appears that insulin stimulates Na-pumping mecha...

    journal_title：Molecular and cellular endocrinology

    pub_type： 杂志文章

    doi：10.1016/0303-7207(86)90087-0

    authors： Baldini P,Incerpi S,Pascale E,Rinaldi C,Verna R,Luly P

    更新日期：1986-07-01 00:00:00

  • Point mutations in follitropin receptor result in ER retention.

    abstract：:Accumulating evidence suggests that the human follitropin receptor is unusually sensitive to mutation. Previous results (Mol. Cell. Endo. 166 (2000) 101) determined that scanning mutations in a.a. 12-14 and 22-30 neither bound follitropin nor were present on the cell surface, suggesting that these regions are involved...

    journal_title：Molecular and cellular endocrinology

    pub_type： 杂志文章

    doi：10.1016/s0303-7207(02)00424-0

    authors： Nechamen CA,Dias JA

    更新日期：2003-03-28 00:00:00

  • The Rac2 GTPase contributes to cathepsin H-mediated protection against cytokine-induced apoptosis in insulin-secreting cells.

    abstract：:The type 1 diabetes (T1D) risk locus on chromosome 15q25.1 harbors the candidate gene CTSH (cathepsin H). We previously demonstrated that CTSH regulates β-cell function in vitro and in vivo. CTSH overexpression protected insulin-secreting INS-1 cells against cytokine-induced apoptosis. The purpose of the present study...

    journal_title：Molecular and cellular endocrinology

    pub_type： 杂志文章

    doi：10.1016/j.mce.2020.110993

    authors： Fløyel T,Mirza AH,Kaur S,Frørup C,Yarani R,Størling J,Pociot F

    更新日期：2020-12-01 00:00:00

  • Effects of activin A on anterior pituitary cells fractionated by centrifugal elutriation.

    abstract：:We have shown previously that activin A increases the number of immunoreactive follicle-stimulating hormone (FSH) cells. To further investigate the action of activin A, we examined its effects on anterior pituitary cells fractionated by centrifugal elutriation. Before activin A treatment, FSH cells were widely distrib...

    journal_title：Molecular and cellular endocrinology

    pub_type： 杂志文章

    doi：10.1016/0303-7207(91)90071-y

    authors： Katayama T,Shiota K,Takahashi M

    更新日期：1991-05-01 00:00:00

  • Epitope-tagged gonadotropin-releasing hormone receptors heterologously-expressed in mammalian (COS-1) and insect (Sf9) cells.

    abstract：:The molecular cloning and nucleotide sequencing of the gonadotropin-releasing hormone (GnRH) receptor represented an enhanced step in the experimental effort to understand this key molecule in the reproductive process at a cell and molecular level. A subsequent step in this broad effort is heterologous expression of t...

    journal_title：Molecular and cellular endocrinology

    pub_type： 杂志文章

    doi：10.1016/s0303-7207(96)04003-8

    authors： Neill JD,Sellers JC,Musgrove LC,Duck LW

    更新日期：1997-03-28 00:00:00

  • 1,25-Dihydroxyvitamin D3 selectively translocates PKCalpha to nuclei in ROS 17/2.8 cells.

    abstract：:We have investigated protein kinase C (PKC) regulation by 1,25-(OH)2D3 in the rat osteosarcoma cell line ROS 17/2.8 since previous reports have implicated PKC in the 1,25-(OH)2D3-mediated regulation of osteocalcin gene expression (J. Biol. Chem. 267 (1992) 12562; Endocrinology 136 (1995) 5685). Here we report that 1,2...

    journal_title：Molecular and cellular endocrinology

    pub_type： 杂志文章

    doi：10.1016/s0303-7207(01)00670-0

    authors： Rivera-Bermúdez MA,Bertics PJ,Albrecht RM,Mosavin R,Mellon WS

    更新日期：2002-02-25 00:00:00

  • Differential involvement of phospholipase A2 in phorbol ester-induced luteinizing hormone and growth hormone release from rat anterior pituitary tissue.

    abstract：:The protein kinase C (PKC) activator, phorbol 12,13-dibutyrate (PDBu) induced the release of both luteinizing hormone (LH) and growth hormone (GH) from proestrous rat anterior pituitary pieces in vitro. Phorbol 12,13-dibutyrate-induced LH, but not GH release was readily inhibited by the phospholipase A2 (PLA2) inhibit...

    journal_title：Molecular and cellular endocrinology

    pub_type： 杂志文章

    doi：10.1016/0303-7207(93)90031-e

    authors： Thomson FJ,Mitchell R

    更新日期：1993-09-01 00:00:00

  • The insulin-induced down-regulation of IRS-1 in 3T3-L1 adipocytes is mediated by a calcium-dependent thiol protease.

    abstract：:Insulin receptor substrate-1 (IRS-1) is a protein expressed in 3T3-L1 adipocytes that is involved in most, if not all of the biological responses to insulin. Chronic exposure of these cells to insulin down-regulates IRS-1 by stimulating its degradation (Rice, K.M., Turnbow, M.A. and Garner, C.W. (1993) Biochem. Biophy...

    journal_title：Molecular and cellular endocrinology

    pub_type： 杂志文章

    doi：10.1016/0303-7207(96)03875-0

    authors： Smith LK,Rice KM,Garner CW

    更新日期：1996-08-30 00:00:00

  • Designing a new generation of anti-hCG vaccines for cancer therapy.

    abstract：:The heterodimeric 'pregnancy-specific' hormone human chorionic gonadotropin (hCG) has been used as the basis for a contraceptive vaccine. More recently, the observation that hCG, particularly in the form of the beta-chain expressed in the absence of alpha-chain, is aberrantly expressed in a number of different tumors ...

    journal_title：Molecular and cellular endocrinology

    pub_type： 杂志文章

    doi：10.1016/j.mce.2006.02.018

    authors： Delves PJ,Iles RK,Roitt IM,Lund T

    更新日期：2007-01-02 00:00:00

  • Calcium influx and DREAM protein are required for GnRH gene expression pulse activity.

    abstract：:Recent evidence using GT1-7 cells indicates that GnRH pulsatility depends on exocytotic-release and gene transcription events. To determine whether calcium or DREAM may play a role in linking these processes, we used an L-type Ca(2+)-blocker (nimodipine) and found that not only GnRH gene expression (GnRH-GE) pulse act...

    journal_title：Molecular and cellular endocrinology

    pub_type： 杂志文章

    doi：10.1016/j.mce.2006.12.040

    authors： Leclerc GM,Boockfor FR

    更新日期：2007-03-15 00:00:00

  • Suppression of follicular cAMP and steroid response to gonadotrophins by pretreatment with gonadotrophin.

    abstract：:This study examines the effect of preincubation of sheep ovarian follicles with hCG or FSH on the ability of isolated theca and granulosa to increase cAMP levels in response to a second incubation with hCG or FSH. Secondly, the effect of preincubation of small follicles with hCG on androstenedione and testosterone pro...

    journal_title：Molecular and cellular endocrinology

    pub_type： 杂志文章

    doi：10.1016/0303-7207(79)90083-2

    authors： Weiss TJ,Armstrong DT

    更新日期：1979-03-01 00:00:00

  • Role of hormone cofactors in the human papillomavirus-induced carcinogenesis of the uterine cervix.

    abstract：:If human papillomavirus (HPV) is necessary for the development of (pre)neoplastic lesions of the uterine cervix, it is not sufficient. Among the cofactors involved in the malignant transformation of cells infected by HPV, sex hormones may facilitate the cervical carcinogenesis by different mechanisms, including the in...

    journal_title：Molecular and cellular endocrinology

    pub_type： 杂志文章,评审

    doi：10.1016/j.mce.2006.10.014

    authors： Delvenne P,Herman L,Kholod N,Caberg JH,Herfs M,Boniver J,Jacobs N,Hubert P

    更新日期：2007-01-29 00:00:00

  • Mice with an RGS-insensitive Gαi2 protein show growth hormone axis dysfunction.

    abstract：:Mice carrying an RGS-insensitive Gαi2 mutation display growth retardation early after birth. Although the growth hormone (GH)-axis is a key endocrine modulator of postnatal growth, its functional state in these mice has not been characterized. The present study was undertaken to address this issue. Results revealed th...

    journal_title：Molecular and cellular endocrinology

    pub_type： 杂志文章

    doi：10.1016/j.mce.2020.111098

    authors： Omouessi ST,Leipprandt JR,Akoume MY,Charbeneau R,Wade S,Neubig RR

    更新日期：2021-02-05 00:00:00

  • Adrenal cholesterol utilization.

    abstract：:There are multiple systems for cellular cholesterol delivery for steroidogenesis, including uptake of lipoprotein-derived cholesterol via LDL receptor mediated endocytic pathways and SR-BI mediated "selective" pathways, as well as from endogenous cholesterol synthesis and the mobilization of stored cholesteryl esters....

    journal_title：Molecular and cellular endocrinology

    pub_type： 杂志文章,评审

    doi：10.1016/j.mce.2006.12.001

    authors： Kraemer FB

    更新日期：2007-02-01 00:00:00

  • Expression of an estrogen receptor alpha variant protein in cell lines and tumors.

    abstract：:Human estrogen receptor alpha (ER) mRNA is a mixture of wild type and alternatively spliced variants. Many studies have examined the potential of ER mRNA profiles to serve as diagnostic/prognostic cancer biomarkers, but only a few have attempted to correlate ER mRNA profiles with protein expression. Representative ER ...

    journal_title：Molecular and cellular endocrinology

    pub_type： 杂志文章

    doi：10.1016/s0303-7207(00)00194-5

    authors： Fasco MJ,Keyomarsi K,Arcaro KF,Gierthy JF

    更新日期：2000-04-25 00:00:00

  • Expression of 17beta- and 3beta-hydroxysteroid dehydrogenases and steroidogenic acute regulatory protein in non-luteinizing bovine granulosa cells in vitro.

    abstract：:Granulosa cells of small follicles differentiate in vitro in serum-free medium, resulting in increased estradiol secretion and abundance of mRNA encoding cytochrome P450aromatase (P450arom). We tested the hypothesis that differentiation in vitro also involves increased expression of 3beta- and 17beta-hydroxysteroid de...

    journal_title：Molecular and cellular endocrinology

    pub_type： 杂志文章

    doi：10.1016/j.mce.2004.05.010

    authors： Sahmi M,Nicola ES,Silva JM,Price CA

    更新日期：2004-08-31 00:00:00