Suppression of follicular cAMP and steroid response to gonadotrophins by pretreatment with gonadotrophin.

Abstract:

:This study examines the effect of preincubation of sheep ovarian follicles with hCG or FSH on the ability of isolated theca and granulosa to increase cAMP levels in response to a second incubation with hCG or FSH. Secondly, the effect of preincubation of small follicles with hCG on androstenedione and testosterone production is examined in intact follicles. Preincubation with hCG resulted in a decrease in the ability of theca from large (4--6 mm in diameter) and small (1--3 mm) follicles and granulosa from large follicles to increase cAMP levels in response to a second exposure to hCG. Similarly, preincubation with FSH suppressed the response of isolated granulosa from small and large follicles to a second exposure to FSH. A partial, but significant inhibition of the response to the other hormone was observed after preincubation with both FSH and hCG. Intact small follicles pretreated with hCG were unable to increase testosterone secretion in response to a second exposure to hCG.

journal_name

Mol Cell Endocrinol

authors

Weiss TJ,Armstrong DT

doi

10.1016/0303-7207(79)90083-2

subject

Has Abstract

pub_date

1979-03-01 00:00:00

pages

229-39

issue

3

eissn

0303-7207

issn

1872-8057

journal_volume

13

pub_type

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