Abstract:
:Recent evidence using GT1-7 cells indicates that GnRH pulsatility depends on exocytotic-release and gene transcription events. To determine whether calcium or DREAM may play a role in linking these processes, we used an L-type Ca(2+)-blocker (nimodipine) and found that not only GnRH gene expression (GnRH-GE) pulse activity was abolished but also that binding of proteins to OCT1BS-a (essential site for GnRH-GE pulses) was reduced. We further found that only EF-hand forms of DREAM were expressed in GT1-7 and that DREAM was part of the complex binding to OCT1BS-a. Finally, microinjection of DREAM antibody into cells abolished GnRH-GE pulses demonstrating its importance in pulsatility. These results reveal that calcium and DREAM may bridge cytoplasmic and nuclear events enabling temporal coordination of intermittent activity. Expression of DREAM in various cell types coupled with the universal role of calcium raise the possibility that these factors may play similar role in other secretory cells.
journal_name
Mol Cell Endocrinoljournal_title
Molecular and cellular endocrinologyauthors
Leclerc GM,Boockfor FRdoi
10.1016/j.mce.2006.12.040subject
Has Abstractpub_date
2007-03-15 00:00:00pages
70-9issue
1-2eissn
0303-7207issn
1872-8057pii
S0303-7207(06)00596-Xjournal_volume
267pub_type
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