Abstract：
:Thyroid dysgenesis is the most frequent cause of congenital hypothyroidism, but its molecular pathophysiology is largely unknown. Our hypothesis that some genes downstream to thyroid transcription factor-2 (TTF-2) might be responsible for development of the thyroid prompted us to identify genes whose expression is stimulated by TTF-2. PCR products of cDNA clones obtained by a subtraction PCR method in TTF-2 expressing cell lines were screened with labeled cDNA by microarray analysis. We isolated 17 genes up-regulated by TTF-2, which were subsequently confirmed by quantitative reverse transcription-polymerase chain reaction (RT-PCR). One of them is a novel gene designated T1560 that showed a highly thyroid-specific expression pattern. Luciferase reporter assays showed that expression of all of the 14 genes tested was stimulated by both TTF-2 and TTF-1, another thyroid-specific transcription factor. Our results have important implications for understanding normal thyroid development as well as the molecular defects underlying thyroid dysgenesis.
journal_name
Mol Cell Endocrinoljournal_title
Molecular and cellular endocrinologyauthors
Hishinuma A,Ohmika N,Namatame T,Ieiri Tdoi
10.1016/j.mce.2004.04.003keywords:
subject
Has Abstractpub_date
2004-06-30 00:00:00pages
33-46issue
1-2eissn
0303-7207issn
1872-8057pii
S0303720704001649journal_volume
221pub_type
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journal_title：Molecular and cellular endocrinology
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pub_type： 杂志文章
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journal_title：Molecular and cellular endocrinology
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