Abstract:
:A full understanding of the key regulators controlling periodontal development and homeostasis is necessary for the design of improved periodontal regenerative therapies. Small leucine-rich proteoglycans (SLRPs) are extracellular matrix molecules suggested to regulate collagen organization and cell signaling. Mice with double-deficiency of 2 SLRPs, fibromodulin and biglycan (dKO), acquire skeletal abnormalities, but their roles in regulating the periodontium remain undefined and were the focus of our studies. Transmission electron microscopy studies showed abnormal collagen fibrils in the periodontal ligament (PDL) and altered remodeling of alveolar bone in dKO mice. Immunohistochemistry (IHC) revealed increased staining of SLRPs (asporin, lumican, and decorin) and dentin matrix protein-1 (DMP1, a mechanosensory/osteocyte marker), while osteoblast markers, bone sialoprotein and osteopontin, remained unchanged. Disruption of homeostasis was further evidenced by increased expression of receptor-activator of nuclear factor-κB ligand (RANKL) and elevated numbers of osteoclasts, especially noted around the alveolar bone of molars (buccal side) and incisors. Polymerase chain reaction (PCR) array revealed hyperactive transforming growth factors beta/bone morphogenetic protein (TGFβ/BMP) signaling in dKO PDL tissues, which was further confirmed by elevated expression of phosphorylated Smad5 (p-Smad5) by IHC in dKO PDL. These studies highlight the importance of SLRPs in maintaining periodontal homeostasis through regulation of TGFβ/BMP signaling, matrix turnover, and collagen organization.
journal_name
J Dent Resjournal_title
Journal of dental researchauthors
Wang L,Foster BL,Kram V,Nociti FH Jr,Zerfas PM,Tran AB,Young MF,Somerman MJdoi
10.1177/0022034514541126subject
Has Abstractpub_date
2014-08-01 00:00:00pages
780-7issue
8eissn
0022-0345issn
1544-0591pii
0022034514541126journal_volume
93pub_type
杂志文章abstract::Twenty-two adults, considered to have high caries activity, rinsed for one yr with either SnF2 or acidulated NaF mouthrinses. Total colony-forming units and numbers of S. mutans and lactobacilli were estimated from saliva samples. Large reductions were found in the number of recoverable S. mutans in subjects rinsing w...
journal_title:Journal of dental research
pub_type: 杂志文章
doi:10.1177/00220345830620081201
更新日期:1983-08-01 00:00:00
abstract::The amount of free water in 50 samples of air-dried enamel from permanent and deciduous teeth was measured by the Karl Fischer method. Samples included both contemporary and burial specimens. The mean values obtained showed that free water constituted about 1% of enamel mass. However, the range of individual values va...
journal_title:Journal of dental research
pub_type: 历史文章,杂志文章
doi:10.1177/00220345880670051701
更新日期:1988-05-01 00:00:00
abstract::The distribution of immunoglobulin-containing cells in human tonsils, salivary glands, and inflamed gingiva is described. The cellular localization of J chain indicates that this peptide is a basic gene product of B cells that is expressed only in the early phase of clonal differentiation. Gland-associated immunocytes...
journal_title:Journal of dental research
pub_type: 杂志文章
doi:10.1177/002203457605500301011
更新日期:1976-04-01 00:00:00
abstract::A double-blind randomized clinical trial was performed in 6- to 7-yr-old schoolchildren to evaluate, in a 30-mo period, whether the caries increment on the distal surface of the second primary molars adjacent to permanent first molars sealed with fluoride release compounds would be lower with respect to those adjacent...
journal_title:Journal of dental research
pub_type: 杂志文章,随机对照试验
doi:10.1177/0022034514535808
更新日期:2014-07-01 00:00:00
abstract::Evidence that dental status affects diet is equivocal. The hypothesis of this study was that diet was affected by dental status. The objective was to assess the relationship between numbers of teeth and diet and nutritional status in US adult civilians without prostheses. We examined 6985 NHANES (1988-1994) participan...
journal_title:Journal of dental research
pub_type: 杂志文章
doi:10.1177/154405910708601206
更新日期:2007-12-01 00:00:00
abstract::Cementum forms the interface between root dentin and periodontal ligament through which periodontal connective tissue is attached to root surfaces. We have examined how cementum components influence the biological activities of gingival fibroblasts. Cementum was harvested from freshly extracted human teeth and extract...
journal_title:Journal of dental research
pub_type: 杂志文章
doi:10.1177/00220345870660082301
更新日期:1987-08-01 00:00:00
abstract::Four disclosant dyes were tested for antimicrobial activity against 27 oral reference strains and five non-oral human pathogens. Erythrosine and fluorescein inhibited most gram-positive and gram-negative organisms associated with dental plaque, and were bactericidal to selected strains. Fast green and brilliant blue d...
journal_title:Journal of dental research
pub_type: 杂志文章
doi:10.1177/00220345830620071601
更新日期:1983-07-01 00:00:00
abstract::Sensory, sympathetic and parasympathetic ganglia located in the head and neck of rhesus monkeys were histologically examined after injection of H3-HRP into the right mandibular premolars and molars. The results showed positive labeling of ganglionic cell bodies located in the ipsilateral trigeminal, superior cervical,...
journal_title:Journal of dental research
pub_type: 杂志文章
doi:10.1177/00220345800590041601
更新日期:1980-04-01 00:00:00
abstract::Although it has been shown that patients are more satisfied with prostheses supported by implants than with conventional dentures, there have been few direct comparisons of the various designs of implant-supported prostheses. This within-subject crossover clinical trial was designed to compare two forms of removable p...
journal_title:Journal of dental research
pub_type: 临床试验,杂志文章
doi:10.1177/00220345970760100901
更新日期:1997-10-01 00:00:00
abstract::Ten cats were immunized with subcutaneous injections of keyhole limpet hemocyanin (KLH). After it was determined that the experimental animals had developed circulating antibodies to KLH, challenge doses of KLH were administered via the root canal system. The radiographic and histologic findings suggest that antigen-a...
journal_title:Journal of dental research
pub_type: 杂志文章
doi:10.1177/00220345800590011601
更新日期:1980-01-01 00:00:00
abstract::The effects of substituting gluconate for extracellular Cl, and of treatment with various ion transport blockers, on cytosol pH (pHi) and secretion by the acetylcholine stimulated rat mandibular gland were studied in vitro. Gluconate replacement increased pHi from 7.12 +/- 0.02 to 7.27 +/- 0.04, caused secretory rate ...
journal_title:Journal of dental research
pub_type: 杂志文章
doi:10.1177/00220345870660022401
更新日期:1987-02-01 00:00:00
abstract::The effect of enamel matrix proteins on the seeded growth of enamel apatite crystals was studied in stable supersaturated solutions at pH 7.4 and 37 degrees C. Of the two major protein classes in the enamel matrix, the enamelins were considerably more effective than the amelogenins in retarding seeded growth. However,...
journal_title:Journal of dental research
pub_type: 杂志文章
doi:10.1177/00220345840630021801
更新日期:1984-02-01 00:00:00
abstract::Diabetes mellitus is associated with depression of natural defenses against infection and increases the risk of periodontal disease. However, the effects of diabetes on periradicular tissue, which differs structurally from periodontal tissue, are not known. In this study, we evaluated the effects of type 2 diabetes on...
journal_title:Journal of dental research
pub_type: 杂志文章
doi:10.1177/154405910308200416
更新日期:2003-04-01 00:00:00
abstract::Occlusal alterations may result in changes in the functional performance of masticatory muscles. In this study, we set up an experimental model in rats to examine whether masticatory muscle abnormalities occur after a malocclusion is induced. Rats underwent unilateral amputation of the molar cusps to simulate an occlu...
journal_title:Journal of dental research
pub_type: 杂志文章
doi:10.1177/00220345990780111101
更新日期:1999-11-01 00:00:00
abstract::Mandibular prognathism is a common dentofacial phenotype with a substantial genetic component; however, few susceptibility loci have been mapped. Ethnicity is a risk factor for mandibular prognathism, and a relatively high prevalence is observed in Asian populations. The hypothesis of this study suggested that a speci...
journal_title:Journal of dental research
pub_type: 杂志文章
doi:10.1177/0022034510382546
更新日期:2011-01-01 00:00:00
abstract::Setting reactions and compressive strengths of a self-hardening calcium phosphate cement (CPC) were investigated. The CPC consists of tetracalcium phosphate (TTCP) and anhydrous dicalcium phosphate (DCPA). The cement specimens were prepared by mixing 0.7 g of the powder (TTCP 72.9 wt% + DCPA 27.1 wt%) with 0.175 mL of...
journal_title:Journal of dental research
pub_type: 杂志文章
doi:10.1177/00220345900690121201
更新日期:1990-12-01 00:00:00
abstract::Samples of dental plaque obtained from the interproximal tooth surfaces of seven young men showed considerable variation in the prevalence and anatomic site distribution of the potentially cariogenic microorganism S mutans. Multiple application of 10% SnF2 topical solution and saline solution delivered to interproxima...
journal_title:Journal of dental research
pub_type: 杂志文章
doi:10.1177/00220345770560010301
更新日期:1977-01-01 00:00:00
abstract::The objectives of this study were 1) to provide an estimate of the value of the intraclass correlation coefficient (ICC) for dental caries data at tooth and surface level, 2) to provide an estimate of the design effect (DE) to be used in the determination of sample size estimates for future dental surveys, and 3) to e...
journal_title:Journal of dental research
pub_type: 杂志文章
doi:10.1177/0022034514559408
更新日期:2015-02-01 00:00:00
abstract::Based on the existing evidence supporting a state of chronic inflammation in chronic kidney disease (CKD), we hypothesized that periodontal infection may affect the systemic inflammatory status of a nationally representative CKD population as measured by serum C-reactive protein (CRP). We examined this hypothesis usin...
journal_title:Journal of dental research
pub_type: 杂志文章
doi:10.1177/0022034511423394
更新日期:2011-12-01 00:00:00
abstract::The remodeling of gingival tissue was measured by determining the incorporation of tritiated proline and the disappearance of tritium activity from proline and hydroxyproline for 18 days after administration of label. There was a rapid decrease in the tritium activity from gingiva which was paralleled by a decrease in...
journal_title:Journal of dental research
pub_type: 杂志文章
doi:10.1177/00220345780570023101
更新日期:1978-02-01 00:00:00
abstract::The benefits of early orthodontic treatment are continuously discussed, but studies are few. We examined whether definite need for orthodontic treatment could be eliminated in public health care by systematically focusing on early intervention. One age cohort living in a rural Finnish municipality (N = 85) was regular...
journal_title:Journal of dental research
pub_type: 杂志文章
doi:10.1177/154405910808700604
更新日期:2008-06-01 00:00:00
abstract::Catecholamines are present in saliva, but their influence on oral epithelium is not understood. Because psychological stress increases salivary catecholamines and impairs oral mucosal wound healing, we sought to determine if epithelial adrenergic signaling could link these two findings. We found that cultured human or...
journal_title:Journal of dental research
pub_type: 杂志文章
doi:10.1177/0022034510388034
更新日期:2011-02-01 00:00:00
abstract::Dental composites develop residual stresses during polymerization due to shrinkage. These stresses may change with time because of relaxation and water sorption in the oral environment. This phenomenon is likely dependent on the composition of the materials, specifically their hydrophilic characteristics, and could re...
journal_title:Journal of dental research
pub_type: 杂志文章
doi:10.1177/0022034513513905
更新日期:2014-02-01 00:00:00
abstract::Eugenol, in the 100 percent concentration in commercial use, was applied to a circumscribed area, 3 mm in diameter, of rat labial mucosa for 1 minute. Reaction periods of 15 minutes, 1, 2, 4 and 6 hours were then permitted. Using routine histological procedures for processing the experimental tissues, it was observed ...
journal_title:Journal of dental research
pub_type: 杂志文章
doi:10.1177/00220345780570110901
更新日期:1978-11-01 00:00:00
abstract::The diagnosis of Severe Acute Respiratory Syndrome Coronavirus 2 (SARS-CoV-2) infection relies on the detection of viral RNA by real-time reverse transcription polymerase chain reaction (rRT-PCR) performed with respiratory specimens, especially nasopharyngeal swabs. However, this procedure requires specialized medical...
journal_title:Journal of dental research
pub_type: 杂志文章
doi:10.1177/0022034520969670
更新日期:2021-02-01 00:00:00
abstract::Enamel fluorosis can occur following either an acute or chronic exposure to fluoride during tooth formation. Fluorosed enamel is characterized by a retention of amelogenins in the early-maturation stage, and by the formation of a more porous enamel with a subsurface hypomineralization. The mechanisms by which fluoride...
journal_title:Journal of dental research
pub_type: 杂志文章,评审
doi:10.1177/00220345920710051701
更新日期:1992-05-01 00:00:00
abstract::Antigenic alterations to the dentin organic matrix may be detected by an immunohistochemical approach. We hypothesized that alterations in the antigenicity of type I collagen and proteoglycans occur in sclerotic dentin under caries lesions. Transverse sections were prepared from carious teeth in the sclerotic zone and...
journal_title:Journal of dental research
pub_type: 杂志文章
doi:10.1177/154405910608500204
更新日期:2006-02-01 00:00:00
abstract::Inflammatory conditions as they occur during periodontal disease often result in decreased alveolar bone levels and a loss of connective tissue homeostasis. Here we have focused on the effect of microRNA-138 (miR-138) as a potential regulator of periodontal stem cells as they affect homeostasis during inflammatory con...
journal_title:Journal of dental research
pub_type: 杂志文章
doi:10.1177/0022034515613043
更新日期:2016-02-01 00:00:00
abstract::The relationship between the serum IgG antibody titer against seven species of Gram-negative periodontopathic bacteria and clinical parameters (including plaque index, gingival index, periodontal pocket depth, and alveolar bone loss) was studied in 38 subjects. IgG antibody titer against the sonicated antigens was det...
journal_title:Journal of dental research
pub_type: 杂志文章
doi:10.1177/00220345850640111101
更新日期:1985-11-01 00:00:00
abstract::The ability of adhesive resins to restore the physical properties of demineralized dentin has not been well-documented. The unfilled resins that are used for adhesion have relatively low moduli of elasticity and limited ability to increase dentin stiffness, although they may increase the ultimate tensile strength of d...
journal_title:Journal of dental research
pub_type: 杂志文章
doi:10.1177/00220345950740041001
更新日期:1995-04-01 00:00:00