Abstract:
:Selected volatile organic compounds (VOCs) in breath may be considered biomarkers if they are indicative of distinct diseases or disease states. Given the inherent molecular selectivity of vibrational spectroscopy, infrared sensing technologies appear ideally suitable for the determination of endogenous VOCs in breath. The aim of this study was to determine that mid-infrared (MIR; 3-20 µm) gas phase sensing is capable of determining isoprene in exhaled breath as an exemplary medically relevant VOC by hyphenating novel substrate-integrated hollow waveguides (iHWG) with a likewise miniaturized preconcentration system. A compact preconcentrator column for sampling isoprene from exhaled breath was coupled to an iHWG serving simultaneously as highly miniaturized gas cell and light conduit in combination with a compact Fourier transform infrared spectrometer. A gas mixing system enabled extensive system calibration using isoprene standards. After system optimization, a calibration function obtaining a limit of quantification of 106 ppb was achieved. According to the literature, the obtained sensitivity is sufficient for quantifying middle to high isoprene concentrations occurring in exhaled breath. Finally, a volunteer breath sample was analysed proving comparable values of isoprene in a real-world scenario. Despite its fundamental utility, the proposed methodology contains some limitations in terms of sensitivity and temporal resolution in comparison with the readily available measurement techniques that should be addressed during future optimization of the system. Nonetheless, this study presents the first determination of endogenous VOCs in breath via advanced hollow waveguide MIR sensor technology, clearly demonstrating its potential for the analysis of volatile biomarkers in exhaled breath.
journal_name
J Breath Resjournal_title
Journal of breath researchauthors
Perez-Guaita D,Kokoric V,Wilk A,Garrigues S,Mizaikoff Bdoi
10.1088/1752-7155/8/2/026003subject
Has Abstractpub_date
2014-06-01 00:00:00pages
026003issue
2eissn
1752-7155issn
1752-7163journal_volume
8pub_type
杂志文章abstract::Fractional exhaled NO (FeNO) is universally considered an indirect marker of eosinophilic airways inflammation, playing an important role in the physiopathology of childhood asthma. Advances in technology and standardization have allowed a wider use of FeNO in clinical practice in children from the age of four years. ...
journal_title:Journal of breath research
pub_type: 杂志文章,评审
doi:10.1088/1752-7155/6/2/027103
更新日期:2012-06-01 00:00:00
abstract::Despite growing interest and considerable progress in breath research over the last decade, standardized practices for the sampling and analysis of breath gas volatiles remain elusive. The primary reasons for this are (a) the rich chemical diversity of exhaled breath that covers an extensive range of volatile organic ...
journal_title:Journal of breath research
pub_type: 杂志文章
doi:10.1088/1752-7155/8/3/037101
更新日期:2014-09-01 00:00:00
abstract::Based on the adsorption of analytes in the sampling loop, a time-resolved dynamic dilution introduction method was developed for negative ion mobility spectrometry to continuously monitor end-tidal propofol without other sample pre-separation. The dynamic dilution characteristics of propofol and moisture in the Teflon...
journal_title:Journal of breath research
pub_type: 杂志文章
doi:10.1088/1752-7155/9/1/016002
更新日期:2015-01-05 00:00:00
abstract::The haloamines, including the chloramines (H(2)NCl, HNCl(2)) and bromamine (H(2)NBr), are diffusible gases that are likely to be produced during inflammation and so may be present as markers of lung inflammation on breath. Although haloamines are quite reactive, it is possible to measure these compounds in humid sampl...
journal_title:Journal of breath research
pub_type: 杂志文章
doi:10.1088/1752-7155/5/3/037105
更新日期:2011-09-01 00:00:00
abstract::There are many respiratory infections such as influenza that cause epidemics. These respiratory infection epidemics can be effectively prevented by determining the presence or absence of infections in patients using frequent tests. We think that self-diagnosis may be possible using a system that can collect and detect...
journal_title:Journal of breath research
pub_type: 杂志文章
doi:10.1088/1752-7155/10/3/036009
更新日期:2016-07-22 00:00:00
abstract::Lung cancer is a leading cause of cancer-related deaths worldwide, and is considered one of the most aggressive human cancers, with a 5 year overall survival of 10-15%. Early diagnosis of lung cancer is ideal; however, it is still uncertain as to what technique will prove successful in the systematic screening of high...
journal_title:Journal of breath research
pub_type: 杂志文章,评审
doi:10.1088/1752-7155/10/3/034001
更新日期:2016-07-05 00:00:00
abstract::Microbial cultures and/or microbial associated diseases often have a characteristic smell. Volatile organic compounds (VOCs) are produced by all microorganisms as part of their normal metabolism. The types and classes of VOC produced is wide, including fatty acids and their derivatives (e.g. hydrocarbons, aliphatic al...
journal_title:Journal of breath research
pub_type: 杂志文章,评审
doi:10.1088/1752-7155/6/2/024001
更新日期:2012-06-01 00:00:00
abstract::Blood ammonia is routinely used in clinical settings to assess systemic ammonia in hepatic encephalopathy and urea cycle disorders. Despite its drawbacks, blood measurement is often used as a comparator in breath studies because it is a standard clinical test. We sought to evaluate sources of measurement error and pot...
journal_title:Journal of breath research
pub_type: 杂志文章
doi:10.1088/1752-7155/9/4/047109
更新日期:2015-12-14 00:00:00
abstract:BACKGROUND:Asthma control does not yet meet the goals of asthma management guidelines. Non-invasive monitoring of airway inflammation may help to improve the level of asthma control in children. OBJECTIVES:(1) To identify a set of exhaled volatile organic compounds (VOCs) that is most predictive for an asthma exacerba...
journal_title:Journal of breath research
pub_type: 杂志文章
doi:10.1088/1752-7163/aa5a8b
更新日期:2017-03-01 00:00:00
abstract::Biogenic amines are interesting compounds which may be of use for medical diagnosis or therapeutic monitoring. The present paper deals with the problems that occur with concentration determination of dimethylamine (DMA) and trimethylamine (TMA). These occur in the breath of people suffering from renal disease. The mea...
journal_title:Journal of breath research
pub_type: 杂志文章
doi:10.1088/1752-7155/4/2/026002
更新日期:2010-06-01 00:00:00
abstract::Poor compliance to prescribed medication regimens in clinical trials continues to be a major obstacle to drug development success and cost reduction, and there is no gold standard technology that is cheap and effective to reliably measure drug compliance. This paper presents a new drug compliance scheme based on the d...
journal_title:Journal of breath research
pub_type: 杂志文章
doi:10.1088/1752-7155/1/2/026006
更新日期:2007-12-01 00:00:00
abstract::Exhaled breath collection is used to identify and monitor inflammatory or oxidative components in breath. Exhaled breath sample collection is noninvasive and would greatly benefit human pollutant exposure research. We demonstrate the efficacy of exhaled breath collection and analysis in two human exposure studies to o...
journal_title:Journal of breath research
pub_type: 杂志文章
doi:10.1088/1752-7155/2/3/037019
更新日期:2008-09-01 00:00:00
abstract::Staphylococcus aureus (S. aureus) is a common bacterium infecting children with cystic fibrosis (CF). Since current detection methods are difficult to perform in children, there is need for an alternative. This proof of concept study investigates whether breath profiles can discriminate between S. aureus infected and ...
journal_title:Journal of breath research
pub_type: 杂志文章
doi:10.1088/1752-7155/10/4/046014
更新日期:2016-11-30 00:00:00
abstract::The data obtained for the concentration distributions of the most abundant volatile metabolites in exhaled breath determined in two independent studies are reviewed, the first limited study involving five healthy volunteers providing daily breath samples over a month, and the subsequent study involving 30 healthy volu...
journal_title:Journal of breath research
pub_type: 杂志文章
doi:10.1088/1752-7155/1/1/014004
更新日期:2007-09-01 00:00:00
abstract::The analysis of exhaled breath is drawing a high degree of interest in the diagnostics of various diseases, including lung cancer. Electronic nose (E-nose) technology is one of the perspective approaches in the field due to its relative simplicity and cost efficiency. The use of an E-nose together with pattern recogni...
journal_title:Journal of breath research
pub_type: 杂志文章
doi:10.1088/1752-7163/ab433d
更新日期:2019-10-23 00:00:00
abstract::Due to its high sensitivity, compact size and low cost ion mobility spectrometry (IMS) has the potential to become a point-of-care breath analyzer. Therefore, we developed a prototype of a compact, closed gas loop IMS with gas chromatographic (GC) pre-separation and high resolving power of R = 90. In this study, we ...
journal_title:Journal of breath research
pub_type: 杂志文章
doi:10.1088/1752-7155/10/2/026004
更新日期:2016-04-08 00:00:00
abstract::In this article, a database of blood:air and fat:air partition coefficients (λ b:a and λ f:a) is reported for estimating 1678 volatile organic compounds recently reported to appear in the volatilome of the healthy human. For this purpose, a quantitative structure-property relationship (QSPR) approach was applied and a...
journal_title:Journal of breath research
pub_type: 杂志文章
doi:10.1088/1752-7155/10/1/017103
更新日期:2016-01-27 00:00:00
abstract::Reactive oxygen species are associated with tissue inflammation and injury. Our laboratory has demonstrated that ethane, a stable product of lipid peroxidation, in exhaled breath can be used to measure total body oxidative stress. Herein patients were studied who underwent outpatient surgery, laproscopic bilateral tub...
journal_title:Journal of breath research
pub_type: 杂志文章
doi:10.1088/1752-7155/3/1/016002
更新日期:2009-03-01 00:00:00
abstract:BACKGROUND:Recent studies have highlighted the possible involvement of leptin in inflammation. The leptin receptor is also expressed by alveolar macrophages, T lymphocytes and bronchial epitelial cells, suggesting a possible role in the cascade of airway inflammation. OBJECTIVES:The aim of the study was to evaluate th...
journal_title:Journal of breath research
pub_type: 杂志文章
doi:10.1088/1752-7163/aa61c5
更新日期:2017-09-27 00:00:00
abstract::The measurement of the peak exhaled breath temperature (EBT) during multiple tidal breaths offers an easy, non-invasive tool for monitoring airway inflammation. Chronic obstructive pulmonary disease (COPD) is linked to airway inflammation, which is further aggravated by exacerbations of the disease. However, the peak ...
journal_title:Journal of breath research
pub_type: 杂志文章
doi:10.1088/1752-7155/8/4/046002
更新日期:2014-09-18 00:00:00
abstract::Robust methods for breath sampling and analysis are required for potential clinical applications. We have evaluated an improved sampling and experimental design, assessing instrumental and biological variability within and across breath measurements. Calibration curves for selected relevant volatile organic compounds...
journal_title:Journal of breath research
pub_type: 杂志文章
doi:10.1088/1752-7155/6/2/026002
更新日期:2012-06-01 00:00:00
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journal_title:Journal of breath research
pub_type: 杂志文章,评审
doi:10.1088/1752-7155/9/2/027001
更新日期:2015-05-06 00:00:00
abstract::Tongue coating (TC) mainly consists of protein mostly from exfoliated epithelial cells. Until now, to reduce TC accumulation, only mechanical measures have been available, and the procedure involves unpleasant side effects, such as gagging reflex or carcinogenesis related to mechanical stimulation. We expected that pr...
journal_title:Journal of breath research
pub_type: 杂志文章,随机对照试验
doi:10.1088/1752-7155/6/1/017107
更新日期:2012-03-01 00:00:00
abstract::Profiling the body chemistry by means of volatile organic compounds (VOCs) in the breath opens exciting new avenues in medical diagnostics. Gas sensors could provide ideal platforms for realizing portable, hand-held breath testing devices in the near future. This review summarizes the latest developments and applicati...
journal_title:Journal of breath research
pub_type: 杂志文章,评审
doi:10.1088/1752-7155/8/2/027103
更新日期:2014-06-01 00:00:00
abstract::Oral malodour is a common condition which affects a large proportion of the population, resulting in social, emotional and psychological stress. Certain oral bacteria form a coating called a biofilm on the tongue dorsum and degrade organic compounds releasing volatile sulfur compounds that are malodourous. Current che...
journal_title:Journal of breath research
pub_type: 杂志文章
doi:10.1088/1752-7155/10/4/046009
更新日期:2016-10-18 00:00:00
abstract::Exhaled breath is coming to the forefront of non-invasive biomarker discovery efforts. Concentration of exhaled breath volatile organic compounds (VOCs) on thermal desorption (TD) tubes with subsequent analysis by gas chromatography-mass spectrometry (GC-MS) has dominated this field. As discovery experimentation incre...
journal_title:Journal of breath research
pub_type: 杂志文章
doi:10.1088/1752-7155/10/4/046008
更新日期:2016-10-12 00:00:00
abstract::The discovery of nitric oxide (NO) as a signalling and regulatory molecule and its subsequent detection in the exhaled breath has not only yielded new mechanistic insights but also diagnostic opportunities and therapeutic targets in several important medical conditions. In diseases involving chronic pulmonary inflamma...
journal_title:Journal of breath research
pub_type: 杂志文章,评审
doi:10.1088/1752-7155/7/1/017118
更新日期:2013-03-01 00:00:00
abstract::Nitrative stress is thought to be involved in the inflammatory process in COPD airways, and the alveolar nitric oxide concentration (CAlv) has been reported to be increased. However, the CAlv levels are also regulated by gas diffusion at alveolar sites. The aim of the study was to assess the relationship between the C...
journal_title:Journal of breath research
pub_type: 杂志文章
doi:10.1088/1752-7155/7/4/046002
更新日期:2013-12-01 00:00:00
abstract::In the current pilot study we aimed to determine whether breath analysis could be used to help recognise intra-abdominal infection, using acute appendicitis as an exemplar condition. Our study included 53 patients (aged 18-88 years) divided into three groups: appendix group, 26 (13 male) patients suffering from acute ...
journal_title:Journal of breath research
pub_type: 杂志文章
doi:10.1088/1752-7163/abba88
更新日期:2020-10-22 00:00:00
abstract::The lung just like all other organs is affected by age. The lung matures by the age of 20 and age-related changes start around middle age, at 40-50 years. Exhaled nitric oxide (FENO) has been shown to be age, height and gender dependent. We hypothesize that the nitric oxide (NO) parameters alveolar NO (CANO), airway f...
journal_title:Journal of breath research
pub_type: 杂志文章
doi:10.1088/1752-7163/aa7957
更新日期:2017-09-13 00:00:00