Abstract：
:The effect of Aclacinomycin B (ACM-B), an anthracycline antitumor antibiotic, on the DNA-dependent RNA synthesis using single- and double-stranded DNAs of known base content and sequence is studied. The data show that ACM-B effectively inhibits the double-stranded DNA-directed RNA synthesis with a preference of poly[d(A-T)] greater than poly[d(G-C)] greater than poly[d(I-C)]. In contrast, it has no inhibitory effect on the template function of single-stranded DNA (e.g. poly dA, poly dT, and poly dC). These results suggest that the mechanism of ACM-B inhibition, like other anthracycline antibiotics, is by intercalation. In addition to the base specificity, there are also dramatic differences in inhibition depending on the base sequence in the DNA template. Thus, ACM-B preferentially inhibits the alternating double-stranded copolymers over the double-stranded homopolymers; e.g. poly[d(A-T)] is inhibited to a greater extent than poly dA.poly dT and poly [d(G-C)] is inhibited more than poly dG.poly dC. Since the inhibition by ACM-B can be totally abolished when assayed in excess amount of DNA, this result suggests that ACM-B inhibition of RNA synthesis is solely on the DNA template (which is in support of the intercalation model), and has ruled out the possibility that ACM-B may also exert an inhibitory effect on the activity of RNA polymerase per se.
journal_name
Mol Cell Biochemjournal_title
Molecular and cellular biochemistryauthors
Li LH,Yu FLdoi
10.1007/BF00225224subject
Has Abstractpub_date
1989-10-05 00:00:00pages
91-7issue
1eissn
0300-8177issn
1573-4919journal_volume
90pub_type
杂志文章abstract：:MicroRNAs (miRNAs) are small noncoding RNAs that have important roles in cancer. The altered expressions of miRNAs and their target genes are frequently detected in various tumors. In this study, downregulation of miR-15a-16 in nonsmall cell lung cancer (NSCLC) was found to be inversely correlated with Cripto. Results...
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doi：10.1007/BF01837058
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更新日期：1998-03-01 00:00:00
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更新日期：2016-06-01 00:00:00
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更新日期：2020-06-01 00:00:00
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