Abstract:
:Lysosomes are a cell organelle type processing antimicrobial activity. Here, we investigate the lysosomal activity in a primary cell line, bovine aortic endothelial cells (BAECs), during cellular aging, based on the antimicrobial activity of lysosomes isolated from BAECs at cell passages 4, 6, 8, and 10. Cytochemical analysis of lysosomes with LysoTracker reagent revealed the number of lysosome-like organelles surrounding the nucleus initially increased drastically in the BAECs and continued increasing gradually until passage 10. The lysosomes isolated from each successive passage of BAECs exhibited increased antimicrobial activity against Escherichia coli, and, in addition, an age-dependent increase in lysosome intensity coincided with increased lysosomal antimicrobial activity.
journal_name
Mol Cell Biochemjournal_title
Molecular and cellular biochemistryauthors
Yoon J,Kim KJ,Choi YW,Shin HS,Kim YH,Min Jdoi
10.1007/s11010-010-0415-8subject
Has Abstractpub_date
2010-07-01 00:00:00pages
175-8issue
1-2eissn
0300-8177issn
1573-4919journal_volume
340pub_type
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