Inhibition of mitophagy decreases survival of Caenorhabditis elegans by increasing protein aggregation.

Abstract:

:Autophagy of mitochondria, i.e., mitophagy, plays a crucial role in coping with stressors in the aging process, metabolic disturbances, and neurological disorders. Impairments of the process might consequently lead to enhanced accumulation of aged and aggregated proteins and reduced cellular integrity in response to stress. In the present study, we used the stress-sensitive mutant mev-1 of Caenorhabditis elegans to assess the effects of the knockdown of mitophagy relevant genes on survival under heat stress, the amount of autophagosomes, and on protein aggregation. RNA interference for dct-1, drp-1, eat-3, fis-1, fzo1, glb-1, pink-1, and pgam-5 all resulted in a significant reduction of survival time at 37 °C. These effects were associated with a decrease in autophagosomal flux of proteins, as indicated by increased accumulation of GFP-tagged SQST-1, and a reduced amount of lysosomes demonstrating that autophagy was hampered. Moreover, the gene knockdowns led to increased levels of reactive oxygen species in mitochondria and an enhanced protein aggregation. In conclusion, our studies show that mitophagy is of central importance to keep mitochondria functional in order to prevent production of excess reactive oxygen species and protein aggregation and finally a reduction of survival under heat stress.

journal_name

Mol Cell Biochem

authors

Civelek M,Mehrkens JF,Carstens NM,Fitzenberger E,Wenzel U

doi

10.1007/s11010-018-3418-5

subject

Has Abstract

pub_date

2019-02-01 00:00:00

pages

123-131

issue

1-2

eissn

0300-8177

issn

1573-4919

pii

10.1007/s11010-018-3418-5

journal_volume

452

pub_type

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