Abstract:
:Genistein is often used as an inhibitor of tyrosine kinases. A less studied side effect of genistein is an inhibition of cyclic AMP-phosphodiesterase (cAMP-PDE) activity resulting in increased cAMP accumulation. The effect of genistein on intracellular cAMP-levels, basal and forskolin-induced, was studied in A549 human airway epithelial cells and compared with the unspecific PDE inhibitor, isobutylmethylxanthine (IBMX). It was shown that genistein (50 microM) increased basal cAMP and potentiated forskolin-induced cAMP accumulation to the same extent as IBMX (100 microM). Thus, the use of genistein in studies on signaling transductions may result in erroneous conclusions since increased cAMP may cause or contribute to the observed effects.
journal_name
Mol Cell Biochemjournal_title
Molecular and cellular biochemistryauthors
Burvall KM,Palmberg L,Larsson Kdoi
10.1023/a:1020655724911keywords:
subject
Has Abstractpub_date
2002-11-01 00:00:00pages
131-3issue
1-2eissn
0300-8177issn
1573-4919journal_volume
240pub_type
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