IL-17A/IL-17RA promotes invasion and activates MMP-2 and MMP-9 expression via p38 MAPK signaling pathway in non-small cell lung cancer.


:The present study is to investigate the effect and mechanism of action of interleukin (IL)-17A and its receptor IL-17RA on non-small cell lung cancer (NSCLC). A total of 139 NSCLC patients were included in the study. NSCLC tissues and tumor-adjacent tissues were collected from the patients. Human NSCLC cell lines H157, H1975, and A549 were used for in vitro studies. MTT assay was performed to determine cell proliferation. Wound healing assay was used to determine cell motility. Transwell assay was carried out to detect migration and invasion. Quantitative real-time polymerase chain reaction was conducted to measure mRNA expression, while Western blotting was used for determine protein expression. Immunohistochemistry was employed to evaluate IL-17RA expression in 139 primary human NSCLC tissues. Levels of IL-17RA in NSCLC tissues were higher than tumor-adjacent normal tissues, and associated with clinical outcomes. Kaplan-Meier survival analysis indicated that NSCLC patients with positive IL-17RA expression had a poor survival. In addition, IL-17A/IL-17RA affected NSCLC cell migration and invasion in vitro. Treatment with IL-17A/IL-17RA increased the expression of MMP-2 and MMP-9 in NSCLC cells. Furthermore, phosphorylation of p38 was enhanced in IL-17RA-overexpressing NSCLC cells. P38 MAPK-specific inhibitor SB203580 suppressed the migration and invasion of NSCLC cells. MMP-2 and MMP-9 were downstream effectors of IL-17RA and p38 signaling pathways. The present study demonstrates that P38 MAPK activity is crucial for IL-17A/IL-17RA to promote NSCLC metastasis. In addition, IL-17A/IL-17RA signaling may be a novel and promising cancer therapeutic target for the treatment of NSCLC.


Mol Cell Biochem


Wu Z,He D,Zhao S,Wang H




Has Abstract


2019-05-01 00:00:00














  • Ganglioside GM3 promotes HGF-stimulated motility of murine hepatoma cell through enhanced phosphorylation of cMet at specific tyrosine sites and PI3K/Akt-mediated migration signaling.

    abstract::Ganglioside GM3 plays a well-documented and important role in the regulation of tumor cell proliferation, invasion, and metastasis by modulating tyrosine kinase growth factor receptors. However, the effect of GM3 on the hepatocyte growth factor receptor (HGFR, cMet) has not been fully delineated. In the current study,...

    journal_title:Molecular and cellular biochemistry

    pub_type: 杂志文章


    authors: Li Y,Huang X,Zhong W,Zhang J,Ma K

    更新日期:2013-10-01 00:00:00

  • Genetic polymorphism of CYP3A5 in Indian chronic myeloid leukemia patients.

    abstract::CYP3A5 is an important genetic contributor to inter-individual differences in CYP3A-dependent clinically important drugs of metabolism and also of various endogenous compounds and environmental contaminants. The CYP3A5*3 allele results in a truncated protein with loss of CYP3A5 expression and CYP3A5*6 is associated wi...

    journal_title:Molecular and cellular biochemistry

    pub_type: 杂志文章


    authors: Bajpai P,Tripathi AK,Agrawal D

    更新日期:2010-03-01 00:00:00

  • Proteolysis activated protein kinase in Dictyostelium discoideum.

    abstract::In the search for MBP phosphorylating activities in Dictyostelium discoideum, we have found a proteolysis-activated protein kinase. This activity which is distributed between the soluble and the particulate fractions of the cell, uses MBP and histone as substrate and has a molecular mass of 140 kDa as detected in an '...

    journal_title:Molecular and cellular biochemistry

    pub_type: 杂志文章


    authors: Nuñez A,Fernández-Renart M

    更新日期:1997-10-01 00:00:00

  • Insulin sensitivity of liver glycogen synthase b into a conversion.

    abstract::Liver glycogen synthase b phosphatase, chromatographically separable from phosphorylase a phosphatase, is decreased in 48-hour alloxan diabetic rats. The phosphatase activities are measured in an in vitro system using exogenous isolated phospho-enzyme as substrates with added phosphatases. Synthase and phosphorylase p...

    journal_title:Molecular and cellular biochemistry

    pub_type: 杂志文章


    authors: Gold AH,Dickemper D,Haverstick DM

    更新日期:1979-05-06 00:00:00

  • Ionizing radiation induced signaling of DNA damage response molecules in RAW 264.7 and CD4⁺ T cells.

    abstract::Ionizing radiation (IR) treatment results in activation of several DNA damage response molecules, such as ataxia telangiectasia, mutated (ATM), and DNA-dependent protein kinase (DNAPK) in mammals that are increasingly recognized for their potential roles in the sensing of DNA damage and initiating the subsequent prote...

    journal_title:Molecular and cellular biochemistry

    pub_type: 杂志文章


    authors: Dhariwala FA,Narang H,Krishna M

    更新日期:2012-04-01 00:00:00

  • Mitochondrial biogenesis: inhibitors of mitochondrial protein synthesis.

    abstract::The effects of erythromycin, chloramphenicol, cycloheximide, pyrimethamine, chromate, cadmium, lead, nickel, 4-nitro-quinoline-1-oxide and thioacetamide on yeast and human cells were studied. Inhibition of the synthesis of mitochondrial proteins resulted in the loss of cytochromes as well as in morphological changes i...

    journal_title:Molecular and cellular biochemistry

    pub_type: 杂志文章


    authors: Wilkie D

    更新日期:1977-02-04 00:00:00

  • HMGb1 promotes scratch wound closure of HaCaT keratinocytes via ERK1/2 activation.

    abstract::HMGb1 is a DNA-binding protein whose role as an extracellular cytokine in inflammation and tissue regeneration has also been reported. Given the importance of keratinocytes in wound healing, we have studied the mechanism of action of HMGb1 on HaCaT keratinocytes during in vitro scratch wound repair. Western blot and c...

    journal_title:Molecular and cellular biochemistry

    pub_type: 杂志文章


    authors: Ranzato E,Patrone M,Pedrazzi M,Burlando B

    更新日期:2009-12-01 00:00:00

  • Effects of streptozotocin-induced diabetes on connexin43 mRNA and protein expression in ventricular muscle.

    abstract::Abnormal QT prolongation with the associated arrhythmias is a significant predictor of mortality in diabetic patients. Gap junctional intercellular communication allows electrical coupling between heart muscle cells. The effects of streptozotocin (STZ)-induced diabetes mellitus on the expression and distribution of co...

    journal_title:Molecular and cellular biochemistry

    pub_type: 杂志文章


    authors: Howarth FC,Chandler NJ,Kharche S,Tellez JO,Greener ID,Yamanushi TT,Billeter R,Boyett MR,Zhang H,Dobrzynski H

    更新日期:2008-12-01 00:00:00

  • Glycogen synthase kinase 3 phosphorylation of different residues in the presence of different factors: analysis on tau protein.

    abstract::Several peptides derived from microtubule-associated tau protein, have been tested as substrates for glycogen synthase kinase 3 (GSK 3). In the absence of cofactors, GSK 3 can modify serines or threonines followed by prolines. In other cases, a phosphorylation in position +4 is required for the phosphorylation of thre...

    journal_title:Molecular and cellular biochemistry

    pub_type: 杂志文章


    authors: Moreno FJ,Muñoz-Montaño JR,Avila J

    更新日期:1996-12-06 00:00:00

  • Proteomics analysis of plasma membrane from liver sinusoidal endothelial cells after partial hepatectomy by an improved two-dimensional electrophoresis.

    abstract::Liver regeneration is an angiogenesis-associated phenomenon. To identify key plasma membrane (PM) proteins of endothelial cells involved in the initiation of angiogenesis during liver regeneration, the PM of liver sinusoidal endothelial cells (LSEC) at 72 h after partial hepatectomy was enriched by an established in v...

    journal_title:Molecular and cellular biochemistry

    pub_type: 杂志文章


    authors: Li X,Xiong L,Xie C,Cao J,Deng H,Lin Y,Cao R,Li J,Chen P,Liang S

    更新日期:2010-11-01 00:00:00

  • The role of stratifin in fibroblast-keratinocyte interaction.

    abstract::Stratifin is a member of 14-3-3 protein family, a highly conserved group of proteins constituted by seven isoforms. They are involved in numerous crucial intracellular functions such as cell cycle and apoptosis, regulation of signal transduction pathways, cellular trafficking, cell proliferation and differentiation, c...

    journal_title:Molecular and cellular biochemistry

    pub_type: 杂志文章,评审


    authors: Medina A,Ghaffari A,Kilani RT,Ghahary A

    更新日期:2007-11-01 00:00:00

  • miR-202 suppresses proliferation and induces apoptosis of osteosarcoma cells by downregulating Gli2.

    abstract::Accumulating data have shown that microRNAs are involved in the pathogenesis of cancer. miR-202 has been confirmed to be downregulated in several types of human cancer. However, the expression and biological role of miR-202 in osteosarcoma (OS) carcinogenesis and progression remain unclear. In this study, we demonstra...

    journal_title:Molecular and cellular biochemistry

    pub_type: 杂志文章


    authors: Sun Z,Zhang T,Hong H,Liu Q,Zhang H

    更新日期:2014-12-01 00:00:00

  • Comparison of the immunological properties of mammalian (rodent), bird, fish, amphibian (toad), and invertebrate (crab) metallothioneins.

    abstract::Immunoassays such as rocket immunoelectrophoresis, western dot blot hybridization, and competitive ELISA analysis were used to estimate and compare homology among fishes (several species), crab, toad, chicken and rodent metallothioneins. The relative abundance of tissue-specific metallothionein in catfish was highest ...

    journal_title:Molecular and cellular biochemistry

    pub_type: 杂志文章


    authors: Chatterjee A,Maiti IB

    更新日期:1990-05-10 00:00:00

  • Down-regulated miR-28-5p in human hepatocellular carcinoma correlated with tumor proliferation and migration by targeting insulin-like growth factor-1 (IGF-1).

    abstract::Hepatocellular carcinoma (HCC) is a rapidly progressing, incurable cancer that frequently spreads to portal vein and lung. New insights are needed to identify therapeutic targets to prevent or retard HCC metastatic progression. Because microRNAs (miRNA) often act as tumor regulators, we investigated their role in prec...

    journal_title:Molecular and cellular biochemistry

    pub_type: 杂志文章


    authors: Shi X,Teng F

    更新日期:2015-10-01 00:00:00

  • Simulated colon fiber metabolome regulates genes involved in cell cycle, apoptosis, and energy metabolism in human colon cancer cells.

    abstract::High level of dietary fiber has been epidemiologically linked to protection against the risk for developing colon cancer. The mechanisms of this protection are not clear. Fermentation of dietary fiber in the colon results in production of for example butyrate that has drawn attention as a chemopreventive agent. Polyde...

    journal_title:Molecular and cellular biochemistry

    pub_type: 杂志文章


    authors: Putaala H,Mäkivuokko H,Tiihonen K,Rautonen N

    更新日期:2011-11-01 00:00:00

  • Inhibition of nitric oxide synthase enhances contractile response of ventricular myocytes from streptozotocin-diabetic rats.

    abstract::The contractile hyporesponsiveness of the streptozotocin diabetic rat heart in vitro to beta-adrenergic agonists is eliminated when the heart is perfused with N(G)-nitro-L-arginine methyl ester (L-NAME), a non-selective inhibitor of nitric oxide synthase (NOS). The following study evaluated the hypothesis that an incr...

    journal_title:Molecular and cellular biochemistry

    pub_type: 杂志文章


    authors: Smith JM,Sondgeroth KB,Wahler GM

    更新日期:2007-06-01 00:00:00

  • A role for guanidino compounds in the brain.

    abstract::Guanidino compounds of guanidinoethanesulfonic acid, guanidinoacetic acid, guanidinosuccinic acid, N-acetylarginine, beta-guanidinopropionic acid, creatinine, gamma-guanidinobutyric acid, arginine, guanidine, methylguanidine, homoarginine and alpha-guanidinoglutaric acid are present in the mammalian brain. These guani...

    journal_title:Molecular and cellular biochemistry

    pub_type: 杂志文章,评审


    authors: Hiramatsu M

    更新日期:2003-02-01 00:00:00

  • Glucocorticoid receptors and lymphocytolysis in normal and neoplastic lymphocytes.

    abstract::When cells of the thymus or mouse leukemias P288 and L1210 are exposed in vitro to the potent synthetic glucocorticoid, 3H-Triamcinolone acetonide, the steroid enters the cells passively and binds to macromolecules in the cytoplasm. At 37 degrees C this hormone-receptor complex enters the nucleus and associates with t...

    journal_title:Molecular and cellular biochemistry

    pub_type: 杂志文章


    authors: Turnell RW,Burton AF

    更新日期:1975-12-31 00:00:00

  • Multifunctional actions of vanadium compounds on insulin signaling pathways: evidence for preferential enhancement of metabolic versus mitogenic effects.

    abstract::The pathophysiologic importance of insulin resistance in diseases such as obesity and diabetes mellitus has led to great interest in defining the mechanism of insulin action as well as the means to overcome the biochemical defects responsible for the resistance. Vanadium compounds have been discovered to mimic many of...

    journal_title:Molecular and cellular biochemistry

    pub_type: 杂志文章,评审


    authors: Fantus IG,Tsiani E

    更新日期:1998-05-01 00:00:00

  • Protein kinase A phosphorylation of the multifunctional protein CAD antagonizes activation by the MAP kinase cascade.

    abstract::The flux through the de novo pyrimidine biosynthetic pathway is controlled by the multifunctional protein CAD, which catalyzes the first three steps. The cell cycle dependent regulation of pyrimidine biosynthesis is a consequence of sequential phosphorylation of CAD Thr456 and Ser1406 by the MAP kinase and PKA cascade...

    journal_title:Molecular and cellular biochemistry

    pub_type: 杂志文章


    authors: Kotsis DH,Masko EM,Sigoillot FD,Di Gregorio R,Guy-Evans HI,Evans DR

    更新日期:2007-07-01 00:00:00

  • Role of reactive oxygen species in cardiovascular aging.

    abstract::Biochemical and structural changes occurring in the myocardium with aging are mainly resulting from the association of a general tissue atrophy with the hypertrophy of the remaining myocytes. Whilst hypertrophy seems to be a compensatory process to the loss of cardiomyocytes and to a mild systolic hypertensive conditi...

    journal_title:Molecular and cellular biochemistry

    pub_type: 杂志文章,评审


    authors: Muscari C,Giaccari A,Giordano E,Clô C,Guarnieri C,Caldarera CM

    更新日期:1996-07-01 00:00:00

  • Vanadate induces the recruitment of GLUT-4 glucose transporter to the plasma membrane of rat adipocytes.

    abstract::In rat adipocytes, the insulin stimulation of the rate of glucose uptake is due, at least partially, to the recruitment of glucose transporter proteins from an intracellular compartment to the plasma membrane. Vanadate is a known insulin mimetic agent and causes an increase in the rate of glucose transport in rat adip...

    journal_title:Molecular and cellular biochemistry

    pub_type: 杂志文章


    authors: Päquet MR,Romanek RJ,Sargeant RJ

    更新日期:1992-02-12 00:00:00

  • Macromolecular crowding and volume perception in dog red cells.

    abstract::To differentiate whether the primary volume signal in dog red cells arises from a change in cell configuration or the concentration and dilution of cell contents, we prepared resealed ghosts that had the same surface area and hemoglobin concentration as intact cells but less than 1/3 their volume. Shrinkage of both in...

    journal_title:Molecular and cellular biochemistry

    pub_type: 杂志文章


    authors: Parker JC,Colclasure GC

    更新日期:1992-09-08 00:00:00

  • Effect of radiotherapy and chemoradiotherapy on circulating antioxidant system of human uterine cervical carcinoma.

    abstract::Circulating lipid peroxide, antioxidant components and the activities of defense enzymes were estimated in uterine cervical carcinoma patients (before and after radiotherapy and radiotherapy combined chemotherapy) and compared with controls. Some of the antioxidant components such as glutathione, vitamin E and seleniu...

    journal_title:Molecular and cellular biochemistry

    pub_type: 临床试验,杂志文章


    authors: Bhuvarahamurthy V,Balasubramanian N,Govindasamy S

    更新日期:1996-05-10 00:00:00

  • Expression of Sp7 in Satb2-induced osteogenic differentiation of mouse bone marrow stromal cells is regulated by microRNA-27a.

    abstract::Satb2 is a special AT-rich binding transcription factor essential for osteoblast differentiation and bone formation. Specific microRNAs (miRNAs) have been identified to regulate the complex process of osteogenic differentiation. It remains unclear how miRNA expressions is changed in the Satb2-induced osteogenic differ...

    journal_title:Molecular and cellular biochemistry

    pub_type: 杂志文章


    authors: Gong Y,Lu J,Yu X,Yu Y

    更新日期:2016-06-01 00:00:00

  • Selenium protects the immature rat heart against ischemia/reperfusion injury.

    abstract::The aim of the study was to find out whether administration of selenium (Se) will protect the immature heart against ischemia/reperfusion.The control pregnant rats were fed laboratory diet (0.237 mg Se/kg diet); experimental rats received 2 ppm Na(2)SeO(3) in the drinking water from the first day of pregnancy until da...

    journal_title:Molecular and cellular biochemistry

    pub_type: 杂志文章


    authors: Ostadalova I,Vobecky M,Chvojkova Z,Mikova D,Hampl V,Wilhelm J,Ostadal B

    更新日期:2007-06-01 00:00:00

  • Characterization of cholinesterase molecular forms in the mucosal cells along the intestine of the chicken.

    abstract::The presence of a butyrylcholinesterase (BuChE, EC in the musocal cells of the chicken intestine was demonstrated by histochemical and biochemical methods. The study of its distribution, along the intestine from duodenum to rectum, showed that the jejuno-ileum possesses the highest activity. Sucrose gradient ...

    journal_title:Molecular and cellular biochemistry

    pub_type: 杂志文章


    authors: Sine JP,Ferrand R,Colas B

    更新日期:1989-01-23 00:00:00

  • Effects of streptozotocin-induced long-term diabetes on parietal cell function and morphology in rats.

    abstract::Gastric pathology is a common complication in diabetes mellitus. The aim of the study was to evaluate the functions and morphological changes of the parietal cells of the rat stomach after streptozotocin-induced diabetes. Diabetes mellitus was induced in Wistar rats by a single intraperitoneal injection of streptozoto...

    journal_title:Molecular and cellular biochemistry

    pub_type: 杂志文章


    authors: Bastaki SM,Adeghate E,Chandranath IS,Amir N,Tariq S,Hameed RS,Adem A

    更新日期:2010-08-01 00:00:00

  • STAT1 mediates cellular senescence induced by angiotensin II and H₂O₂ in human glomerular mesangial cells.

    abstract::Human glomerular mesangial cells (HMCs) have a finite lifespan and eventually enter irreversible growth arrest known as cellular senescence which is thought to contribute to kidney aging and age-related kidney disorders, such as chronic kidney disease. The signal transducer and activator of transcription 1 (STAT1) is ...

    journal_title:Molecular and cellular biochemistry

    pub_type: 杂志文章


    authors: Jiao S,Meng F,Zhang J,Yang X,Zheng X,Wang L

    更新日期:2012-06-01 00:00:00

  • Effect of p58GTA on beta-1,4-galactosyltransferase 1 activity and cell-cycle in human hepatocarcinoma cells.

    abstract::Beta-1,4-galactosyltransferase 1 (beta1,4-GT 1) is the key enzyme transferring galactose to the terminal N-acetylglucosamine (GlcNAc) forming Galbeta3-->4GlcNAc structure in the Golgi apparatus. In addition, it also serves as a cell adhesion molecule by recognizing and binding to terminal GlcNAc of glycoconjugates on ...

    journal_title:Molecular and cellular biochemistry

    pub_type: 杂志文章


    authors: Zhang SW,Xu SL,Cai MM,Yan J,Zhu XY,Hu Y,Gu JX

    更新日期:2001-05-01 00:00:00